Structure of PDB 7bt6 Chain O Binding Site BS01

Receptor Information

>7bt6 Chain O (length=197) Species: 559292 (Saccharomyces cerevisiae S288C)

VEPVVVIDGKGHLVGRLASVVAKQLLNGQKIVVVRAEELNISGEFFRNKL
KYHDFLRKATAFNKTRGPFHFRAPSRIFYKALRGMVSHKTARGKAALERL
KVFEGIPPPYDKKKRVVVPQALRVLRLKPGRKYTTLGKLSTSVGWKYEDV
VAKLEAKRKVSSAEYYAKKRAFTKKVASANATAAESDVAKQLAALGY

Ligand information

>7bt6 Chain 1 (length=2962)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaac
aaguacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaag
uacgugaaauuguugaaagggaagggcauuugaucagacaggccagcauc
aguuuugguggcaggauaaauccauaggaauguagcuugccucgguaagu
auuauagccugugggaauacugccagcugggacugaggacugcgacguaa
gucaaggaugcuggcauaaugguuauaugccgcccgucuugaaacacgga
ccaaggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcg
uaaugaaagugaacguagguuggggccucgcaagaggugcacaaucgacc
gauccugaugucuucggauggauuugaguaagagcauagcuguugggacc
cgaaagauggugaacuaugccugaauagggugaagccagaggaaacucug
guggaggcucguagcgguucugacgugcaaaucgaucgucgaauuugggu
auaggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaag
uuucccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaa
aacuuuaaauauguaagaaguccuuguuugaacguggacauuugaaugaa
gagcuuuuagugggccauuuuugguaagcagaacuggcgaugcgggauga
accgaacguagaguuaaggugccggaauacacgcucaucagacaccacaa
aagguguuaguucaucuagacagccggacgguggccauggaagucggaau
ccgcuaaggaguguguaacaacucaccggccgaaugaacuagcccugaaa
auggauggcgcucaagcguguuaccuauacucuaccgucaggguugauau
gaugcccugacgaguaggcaggcguggaggucagugacgaagccuagacc
guaaggucgggucgaacggccucuagugcagaucuuggugguaguagcaa
auauucaaaugagaacuuugaagacugaaguggggaaagguuccacguca
acagcaguuggacguggguuagucgauccuaagagauggggaagcuccgu
uuccaucgaaagggaauccgguuaagauuccggaaccuggauauggauuc
uucacgguaacguaacugaauguggagacgucggcgcgagcccugggagg
aguuaucuuuucuucuuaacagcuuaucaccccggaauugguuuauccgg
agauggggucuuauggcuggaagaggccagcaccuuugcuggcuccggug
cgcuugugacggcccgugaaaauccacaggaaggaauaguuuucaugcca
ggucguacugauaaccgcagcaggucuccaaggugaacagccucuaguug
auagaauaauguagauaagggaagucggcaaaauagauccguaacuucgg
gauaaggauuggcucuaagggucggacgaucaacuuagaacugguacgga
caaggggaaucugacugucuaauuaaaacauagcauugcgauggucagaa
agugauguugacgcaaugugauuucugcccagugcucugaaugucaaagu
gaagaaauucaaccaagcgcggguaaacggcgggaguaacuaugacucuc
uuaagguagccaaauggauuaacgagauucccacugucccuaucuacuau
cuagcgaaaccacagccaagggggcuuggcagaaucagcggggaaagaag
acccuguugagcuugacucuaguuugacauugugaagagacauaguuucu
uuacuuauucaagacauugucagguggggaguuugggggcggcacaucug
uuaaacgauaacgcagauguccuaaggggggcucauggagaacagaaauc
uccaguagaacaaaaggguaaaagcccccuugauuuugauuuucagugug
aauacaaaccaugaaaguguggccuaucgauccuuuagucccucggaauu
ugaggcuagaggugccagaaaaguuaccacagggauaacuggcuuguggc
agucaagcguucauagcgacauugcuuuuugauucuucgaugucggcucu
uccuaucauaccgaagcagaauucgguaagcguuggauuguucacccacu
aauagggagugagcuggguuuagaccgucgugagacagguuaguuuuacc
cuacugaugaauguuaccgcaauaguaauugaacuuaguacgagaggaac
aguucauucggauaauugguuuuugcggcugucugaucaggcauugccgc
gaagcuaccauccgcuggauuauggcugaacgccucuaagucagaaucca
ugcuagaacgcggugauuucuuugcuccacacaauauagauggauacgaa
uaaggcguccuuguggcgucgcugaaccauagcaggcuagcaacggugca
cuuggcggaaaggccuugggugcuugcuggcgaauugcaaugucauuuug
cguggggauaaaucauuuguauacgacuuagauguacaacgggguauugu
aagcaguagaguagccuuguuguuacgaucugcugagauuaagccuuugu
ugucugauuugu

..........................<<<<<<.....<<....>>.....
.>>>>>.>.........<<....>>..<<<<<......<<.....>>...
..>>>>>..[[[..<<.......<<.<<<<<<...>>>>>>.>>......
.>>....<<<<<..<<<<<..<<<<<<<<<<<<<<<....<<<<......
>>>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>....
..>>>>>.>>>>>........<<<<<<<.<....>.>>>>>>>.....((
(((..<<<<<<)))))..]]].............................
.....<<....>>...............<<<<....>>>>..........
........................<<<<<.<<<<<<.....<<<<<<<.<
<<<<<<<<<<<<<<.....<<<<<<<<<......<<<<<<....>>>>>>
.......>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....
>>>.....>>>>>>>....>>>>>>.>>>>>..<<<<........>>>><
<<<<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>.
..<<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<
<<<<<....<....>....>>>>.>>.>>....>>>>>>.>>>>>>..<<
.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>
>>.....>>>...<<<<.<<.<<........>>>>>>>>....>>>>>>>
>>...<<.......>>......>>>>>>......>.>>..<<<<<<....
<.<<<....>>>.><<.<<.<......<<<.<<<<<<<............
...........>>>>>...<<<<..<<....>>..>>>>.....>>>>>.
....>.>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<.....
.>>>...<<<<<<<..<<<<(((.....<<<<<<.....<<.........
.<<<.<<<<<<<<<<.......<<<<<.<<....<<.........>>...
.>>.........<<......>>...>>>>>...>>>>>>>>>>>>>....
......))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<......
...>>>>>>>>>....>>>>>><.<<<<<<<..<<......<<<<.<<<<
....>>>>>>>>...>>.>>>>>>>..>.....>>>>>><<<....<<..
...<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<<<<<..
.<<.....>>.>>>>>>....((<<<<<<...<<....<<<....>>>..
.....>>....>>...<<<<<........>>>>>))<<<<.<<<<<<<..
...<<<....>>>.......<<<<<<...<<<<.<<<<<<<.<<<<<<<<
<<..{{{..>>>>>><...<<<<<....<<<<<<....<<<.....>>>.
...>>>>>>.....>>>>>....><<<<<<<......>>>>>>>>>>>..
.>>>>>>>.>>>>.........>>>>>>..........>>>..>>>>>>>
>>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>.
.<<<<.....<<<<<<<.<<.........<.....<<<<<....<<..>>
.....>>>>><<.<<<<<<<<<<<..>>>>>..>>>>>>.>>........
....<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<...
..>>>>......>>>>>>>...............<<.............<
<<<....>>>>..........>>...........<<.........>>...
..........<<..>>......>>.>>>>>>>..>..>>.>>>>>>>..>
>>>>>...>>>....<<<<<....>>>>>....[[[[[..{{{.......
.((((((..[[[[..<<<<<..<<.<.<<..<<<<<.............>
>>>>...<...>.....>>.>.>>...>>>>>......<.<..<<<<<<<
<...........>>>>>>>>.....<<<<<<<...<<<<<<.......>>
>>>>.....<<......>>....>>>>>>>...<<<.<<<<<.......>
>>>.>>>>.<<<....>>>...>.>...[...(((((...((((.>>>>>
>)))).)))))....].....]]]]...))))))..............<<
<<<...<<.......>>...>>>>>.................<<<<<...
<<<<..<.<<<<<<<......>>>>>>>...>.<<<.........>>>..
...>>>>.....>>>>>.....<<<<<<<....>>>.>>>>........}
}}.]]]]]....<<<<<<<<..<<<<.<.<<<<<<...............
>>>>>>.><<<<<...<<<<....<<<<<<<<<.}}}..>>>>..>>>>>
...>>>>..>>>>>.<<<<...<<<<<...........>>>>>...>>>>
.>>>>....>>>>>>>>.......<<<<<<.<<<.<.....<<.......
...<<<<<<.....>>>>>>......>>.......<<<<<<...<<<<<.
<..<<<......>>>..>.>>>>>.>>>>>>..............>.>>>
.>>>>>>..<<<<<<<.............>.....<<<<<<<<<.<<<..
..<<<<.<..<<<<<......>>>>>..>>>>>...>>>....>>>>.>>
>>>.>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7bt6 Structural insights into assembly of the ribosomal nascent polypeptide exit tunnel.
• Resolution: 3.12 Å
• Binding residue (original residue number in PDB): G17 R18 S21 K25 R37 S44 F48 R49 L52 R59 K60 A61 T62 A63 N65 R68 P70 F71 H72 F73 R74 A83 G86 M87 K91 R94 K96 R101 F105 K114 K115 Q122 V126 L129 K130 R133 Y149 L156 R160 S164 Y167 Y168 K171
• Binding residue (residue number reindexed from 1): G15 R16 S19 K23 R35 S42 F46 R47 L50 R57 K58 A59 T60 A61 N63 R66 P68 F69 H70 F71 R72 A81 G84 M85 K89 R92 K94 R99 F103 K112 K113 Q120 V124 L127 K128 R131 Y147 L154 R158 S162 Y165 Y166 K169

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000470 maturation of LSU-rRNA
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0017148 negative regulation of translation

Cellular Component

• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7bt6, PDBe:7bt6, PDBj:7bt6
• PDBsum: 7bt6
• PubMed: 33037216
• UniProt: P26784|RL16A_YEAST Large ribosomal subunit protein uL13A (Gene Name=RPL16A)