Structure of PDB 5uz4 Chain O Binding Site BS01

Receptor Information

>5uz4 Chain O (length=86) Species: 562 (Escherichia coli)

STEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFAEHKKDHHSR
RGLLRMVSQRRKLLDYLKRKDVARYTRLIERLGLRR

Ligand information

>5uz4 Chain A (length=1527)

gaagaguuugaucauggcucagauugaacgcuggcggcaggccuaacaca
ugcaagucgaacgguaacaggaagaagcuugcuucuuugcugacgagugg
cggacgggugaguaaugucugggaaacugccugauggagggggauaacua
cuggaaacgguagcuaauaccgcauaacgucgcaagaccaaagaggggga
ccuucgggccucuugccaucggaugugcccagaugggauuagcuaguagg
ugggguaacggcucaccuaggcgacgaucccuagcuggucugagaggaug
accagccacacuggaacugagacacgguccagacuccuacgggaggcagc
aguggggaauauugcacaaugggcgcaagccugaugcagccaugccgcgu
guaugaagaaggccuucggguuguaaaguacuuucagcggggaggaaggg
aguaaaguuaauaccuuugcucauugacguuacccgcagaagaagcaccg
gcuaacuccgugccagcagccgcgguaauacggagggugcaagcguuaau
cggaauuacugggcguaaagcgcacgcaggcgguuuguuaagucagaugu
gaaauccccgggcucaaccugggaacugcaucugauacuagcaagcuuga
gucucguagagggggguagaauuccagguguagcggugaaaugcguagag
aucuggaggaauaccgguggcgaaggcggcccccuggacgaagacugacg
cucaggugcgaaagcguggggagcaaacaggauuagauacccugguaguc
cacgccguaaacgaugucgacuuggagguugugcccuugaggcguggcuu
ccggagcuaacgcguuaagucgaccgccuggggaguacggccgcaagguu
aaaacucaaaugaauugacgggggcccgcacaagcgguggagcauguggu
uuaauucgaugcaacgcgaagaaccuuaccuggucuugacauccacggaa
guuuucagagaugagaaugugccuucgggaaccgugagacaggugcugca
uggcugucgucagcucguguugugaaauguuggguuaagucccgcaacga
gcgcaacccuuauccuuuguugccagcgguccggccgggaacucaaagga
gacugccagugauaaacuggaggaagguggggaugacgucaagucaucau
ggcccuuacgaccagggcuacacacgugcuacaauggcgcauacaaagag
aagcgaccucgcgagagcaagcggaccucauaaagugcgucguaguccgg
auuggagucugcaacucgacuccaugaagucggaaucgcuaguaaucgug
gaucagaaugccacggugaauacguucccgggccuuguacacaccgcccg
ucacaccaugggaguggguugcaaaagaaguagguagcuuaaccuucggg
agggcgcuuaccacuuugugauucaugacuggggugaagucguaacaagg
uaaccguaggggaaccugcgguuggau

...<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....<<<.
<<<..<<<...<.<...<<<<<<<<<<....>>>>>>>.>>>.>..>.>>
>......<<.......<<<<<<<..<<...<<<<<<<.<.<<.....<<<
<<......>>>>>......>>.>.....<<<....>>>....<<<<<<..
<<....>>>>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<...<<<
<<<.........>>>>>>.>>>......>>>..<<<<<<<<....>>>>.
..>>>>.>>.<<<<.<<.........>>>>>>.<<<<....>>>>...>>
>>>>.........<<<....<<<<....>>>>..>>>..>>.>>>>>>..
<<<<......<<<<....>>>>.....>>>>...<.<<<<<......<.<
<<<<<.<.<...>.>.>>>>>>.>........>>>>>....>..<<<<<(
((...<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>>>>>>
>..........<<<((.....<<<<...<<<..<<<<<<.<<<<<<<<<<
......<<<<<<.....>>>>>>....>>>>>>>>..>>>>>>>>....<
<<<<<<<...<<<<<<<....<<<<<<<<...<<<......>>>......
>>>>>>>>...........<<....>>.>>>>>>>..>>>>.>>>>...>
>>...>>>>....<..<<<...<<...<<<<.........>>>>...>>>
>>..>..........<<<<<<.<<<<<<<<<<<<<.....>>>>>>>>>>
>>>..<<..))>>.....>>>>>>.>>>.<<<......<<<<....>>>>
....>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<...<<<<<<<<..
......<<........>>..........<<<<<<<......<<<<<<<..
<<<<<<<....>>>>>>>...<<....>>..>>>>>.>>.<<<.<<<..<
<<<<<.......<<<<<<<<<....>>>..<<<<......>>>>..>>>>
>>.....<<<..<<<<<<....<...<<<.....>>>.>.....>>>>>>
.....<<<<<.....>>>>>.........>>>.........>>>...>>>
>>>>>>...>>>>>>>......>>>>>>>>.....<<<<<<<.....<<<
..<<...<<<....>>>...>>....>>>.....>>>>>>>......<..
..<<<<<<<........>>>>>>>....>......>>>>>.....<<<<<
<.........>>>>>>.......>>...>>>>>>>>>>.>>........<
<..<<<<.<....<<<<<<<..<<<....<<<<<..<<<...<<....>>
..>>>.>>>>>...>>>..>>>>>>>....>.>>>>..>.>.........
.<<<<<<<<<....>>>>>>>>>....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5uz4 The cryo-EM structure of YjeQ bound to the 30S subunit suggests a fidelity checkpoint function for this protein in ribosome assembly.
• Resolution: 5.8 Å
• Binding residue (original residue number in PDB): T7 N19 D20 T21 G22 Q27 H37 L38 H41 E44 H45 K47 D48 H50 S51 R53 R57 M58 Q61 R63 K64 Y68
• Binding residue (residue number reindexed from 1): T5 N17 D18 T19 G20 Q25 H35 L36 H39 E42 H43 K45 D46 H48 S49 R51 R55 M56 Q59 R61 K62 Y66

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding
• GO:0070181 small ribosomal subunit rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0006417 regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5uz4, PDBe:5uz4, PDBj:5uz4
• PDBsum: 5uz4
• PubMed: 28396444
• UniProt: P0ADZ4|RS15_ECOLI Small ribosomal subunit protein uS15 (Gene Name=rpsO)