Structure of PDB 3j81 Chain O Binding Site BS01

Receptor Information

>3j81 Chain O (length=127) Species: 28985 (Kluyveromyces lactis)

SQVFGVARIFASFNDTFVHVTDLSGRETIARVTGGMKVKADRDESSPYAA
MLAAQDVAAKCKEVGITAVHIKIRATGGTRSKTPGPGGQAALRALARSGL
RIGRIEDVTPVPSDSTRKKGGRRGRRL

Ligand information

>3j81 Chain 2 (length=1780)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacauggauaucug
ugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaagag
auguauuuauuagauaaaaaaucaaugucuucggacuccuugaugauuca
uaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaaa
uuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuuc
aacggguaacggggaauaaggguucgauuccggagagggagccugagaaa
cggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccua
auucagggagguagugacaauaaauaacgauacagggcccauucgggucu
uguaauuggaaugaguacaauguaaauaccuuaacgaggaacaacuggag
ggcaagucuggugccagcagccgcgguaauuccagcuccaguagcguaua
uuaaaguuguugcaguuaaaaagcucguaguugaacuuugggucugguug
uccggucgguuuuucaaccggaucuuuccuucuggcuaaccuguacuccu
ugugggugcaggcgaaccaggacuuuuacuuugaaaaaauuagaguguuc
aaagcaggcgaaagcucgaauauauuagcauggaauaauggaauaggacg
uuugguucuauuuuguugguuucuaggaccaucguaaugauuaauaggga
cggucgggggcaucaguauucaauugucagaggugaaauucuuggauuua
uugaagacuaacuacugcgaaagcauuugccaaggacguuuucauuaauc
aagaacgaaaguuaggggaucgaagaugaucagauaccgucguagucuua
accauaaacuaugccgacuagggaucgggugguguuuuucuuaugaccca
cucggcaccuuacgagaaaucaaagucuuuggguucuggggggaguaugg
ucgcaaggcugaaacuuaaaggaauugacggaagggcaccaccaggagug
gagccugcggcuuaauuugacucaacacggggaaacucaccagguccaga
cacaauaaggauugacagauugagagcucuuucuugauuuuguggguggu
ggugcauggccguucuuaguugguggagugauuugucugcuuaauugcga
uaacgaacgagaccuuaaccuacuaaauaggguugcuggcacuugccggu
ugacucuucuuagagggacuaucgguuucaagccgauggaaguuugaggc
aauaacaggucugugaugcccuuagacguucugggccgcacgcgcgcuac
acugacggagccagcgaguacaaccuuggccgagaggucuggguaaucuu
gugaaacuccgucgugcuggggauagagcauuguaauuauugcucuucaa
cgaggaauuccuaguaagcgcaagucaucagcuugcguugauuacguccc
ugcccuuuguacacaccgcccgucgcuaguaccgauugaauggcuuagug
aggccucaggauuugcuuagagaagggggcaacuccaucucagagcgaag
aaucuggucaaacuuggucauuuagaggaacuaaaagucguaacaagguu
uccguaggugaaccugcggaaggaucauua

...<<<<<...[[[[>>>>>[[[..((((((................[[[
.[[[..<<....<<....<<..........>>...>>.>>......{{..
......[[[..{{..{{....[[[..................<.<..<<.
<<.......>>.>>.....>>.........<<<<<..<<....>>..>>>
>>[...((((((......<<<<<<<<<<<....>>>..............
)))))).....]...<<<<..<<<.....>>>.>>>>....]]]...}}}
}..]]].<<<....<<<....<<<<<<<<.......>>>>>>>>>>>...
...>>>...<<<.<<<<....>>>>....>>>.}}.<<.<<<........
..>>>.>>.<.<<....>>..>..]]]]]].........<<<....<<<.
....>>>..>>>...............<<.<<<<<<............>>
>>>>..>.>.....<<.................>>.........<<<<<(
(......<<<<.....<<..))>>.......>>>>.>>>>>..)))))).
]]].........[[[{{.......{{...[[[.[[.....<<<<<<<<<<
.....<<>>.....>>>>>>>>>.>....<<<<<<....<.<.<..<...
....>..>.>.>....>>>>>>...<<<<<.<<.......<<...<....
...>..<<.......>>...>>......>>.>>..>>>.........[[.
..((((((((....>>>>>>>>.))))))))..]].....]]....<<<<
<<.<<...<<<<..<<..<<<<<<.<...<<<......>>>......>.>
>>>>>..>>.......<<....>>...>>>>...>>>>>.>>>...]]].
..}}......<<<<<<<...<...<<<<.<.....>.>>>>...>>>>>>
>>..........<<<.<<.<<<..<<<<<<<<.<<.<.......>>>>>>
>>>>>..>>>...<<..}}>>...>>.....>>>.]]].<<<......<<
<<....>>>>....>>>..]]]]..<<<<<.<<<<<<<..<<.<<<<<<.
.<<<.<<<<<<......<<........>>..........<<<<<.<....
<<<<<.....<..<<..<<........>.>.>>.>....>>>>>...<<.
<<<..<<.<<<<<<....<<<.<<<<......>>...<<<......>>>.
..>>.>>>....<<<<<.<<..<<<<..<<<<<<<<<<<<....>>>>>>
.>>>>>>..>>>>.>>....<<<<<<.....>>>>>>.......>>>>>.
...>>>.>>>.....>>>>>>>.....>.>>>>>...>>.>>>>.>>>..
...<<<<<<<......<<.....<<<.<<<<....>>>.>>>>....>>.
......>>>>>>>......<....<<<<<<..........>>>>>>....
>.....>>>>>>....<<<<<<<<.......>>>>>>>>......>>...
>>>>>>>>>>.>>....<..<<.<..<<<<.<<....<<.<<<.<.<<<.
.<<<<..<...<<<<<....<..................>....>>>>>.
..>..>>>>..>>>.>.>>>.>>...>>.>>>>...>.>>...>.....<
<<<<<<<<<..>>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3j81 Structural changes enable start codon recognition by the eukaryotic translation initiation complex.
• Resolution: 4.0 Å
• Binding residue (original residue number in PDB): R18 N24 D25 F27 H29 G35 R36 E37 R41 T43 G45 M46 D51 R52 E54 R84 G88 R90 V121 P122 S123 D124 T126 R127 K129 G131 R132 R133 R135 R136
• Binding residue (residue number reindexed from 1): R8 N14 D15 F17 H19 G25 R26 E27 R31 T33 G35 M36 D41 R42 E44 R74 G78 R80 V111 P112 S113 D114 T116 R117 K119 G121 R122 R123 R125 R126

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:3j81, PDBe:3j81, PDBj:3j81
• PDBsum: 3j81
• PubMed: 25417110
• UniProt: P27069|RS14_KLULA Small ribosomal subunit protein uS11 (Gene Name=RPS14)