Structure of PDB 2qa4 Chain O Binding Site BS01

Receptor Information

>2qa4 Chain O (length=115) Species: 2238 (Haloarcula marismortui)

SKTNPRLSSLIADLKSAARSSGGAVWGDVAERLEKPRRTHAEVNLGRIER
YAQEDETVVVPGKVLGSGVLQKDVTVAAVDFSGTAETKIDQVGEAVSLEQ
AIENNPEGSHVRVIR

Ligand information

>2qa4 Chain 0 (length=2753)

uaugccagcugguggauugcucggcucaggcgcugaugaaggacgugcca
agcugcgauaagccauggggagccgcacggaggcgaagaaccauggauuu
ccgaaugagaaucucuaacaauugcuucgcgcaaugaggaaccccgagaa
cugaaacaucucaguaucgggaggaacagaaaacgcaaugugaugucguu
aguaaccgcgagugaacgcgauacagcccaaaccgaagcccucacgggca
auguggugucagggcuaccucucaucagccgaccgucucgacgaagucuc
uuggaacagagcgugauacagggugacaaccccguacucgagaccaguac
gacgugcgguagugccagaguagcggggguuggauaucccucgcgaauaa
cgcaggcaucgacugcgaaggcuaaacacaaccugagaccgauagugaac
aaguagugugaacgaacgcugcaaaguacccucagaagggaggcgaaaua
gagcaugaaaucaguuggcgaucgagcgacagggcauacaaggucccucg
acgaaugaccgacgcgcgagcguccaguaagacucacgggaagccgaugu
ucugucguacguuuugaaaacgagccagggagugugucugcauggcaagu
cuaaccggaguauccggggaggcacagggaaaccgacauggccgcagggc
uugcccgagggccgccgucuucaagggcggggagccauguggacacgacc
cgaauccggacgaucuacgcauggacaagaugaagcgugccgaaaggcac
guggaagucuguuagaguugguguccuacaauacccucucgugaucuaug
uguaggggugaaaggcccaucgaguccggcaacagcugguuccaaucgaa
acaugucgaagcaugaccuccgccgagguagucugugagguagagcgacc
gauugguccugucaaacuccaaacuuacagacgccguuugacgcggggau
uccggugcgcgggguaagccuguguaccaggaggggaacaacccagagau
agguuaagguccccaaguguggauuaaguguaauccucugaagguggucu
cgagcccuagacagccgggaggugagcuuagaagcagcuacccucuaaga
aaagcguaacagcuuaccggccgagguuugaggcgcccaaaaugaucggg
acucaaauccaccaccgagaccuguccguaccacucauacugguaaucga
guagauuggcgcucuaauuggauggaaguaggggugaaaacuccuaugga
ccgauuagugacgaaaauccuggccauaguagcagcgauagucgggugag
aaccccgacggccuaauggauaaggguuccucagcacugcugaucagcug
aggguuagccgguccuaagucauaccgcaacucgacuaugacgaaauggg
aaacggguuaauauucccgugccacuaugcagugaaaguugacgcccugg
ggucgaucacgcugggcaucgcccagucgaaccguccaacuccguggaag
ccguaauggcaggaagcggacgaacggcggcauagggaaacgugauucaa
ccuggggcccaugaaaagacgagcauaguguccguaccgagaaccgacac
agguguccauggcggcgaaagccaaggccugucgggagcaaccaacguua
gggaauucggcaaguuagucccguaccuucggaagaagggaugccugcuc
cggaacggagcaggucgcagugacucggaagcucggacugucuaguaaca
acauaggugaccgcaaauccgcaaggacucguacggucacugaauccugc
ccagugcagguaucugaacaccucguacaagaggacgaaggaccugucaa
cggcgggggucuuaagguagcguaguaccuugccgcaucaguagcggcuu
gcaugaauggauuaaccagagcuucacugucccaacguugggcccgguga
acuguacauuccagugcggagucuggagacacccagggggaagcgaagac
ccuauggagcuuuacugcaggcugucgcugaggacucucacuccgggagg
aggacaccgauagccgggcaguuugacuggggcgguacgcgcucgaaaag
auaucgagcgcgcccuauggcuaucucagccggggacccggcgaagagug
caagagcaaaagauagcuugacaguguucuucccaacgaggaacgcugac
gcgaaagcguggucuagcgaaccaauuagccugcuugaugcgggcaauug
augacagaaaagcuacccuagggauaacagagucgucacucgcaagagca
cauaucgaccgaguggcuugcuaccucgaugucgguucccuccauccugc
ccgugcagaagcgggcaagggugagguuguucgccuauuaaaggaggucg
ugagcuggguuuagaccgucgugagacaggucggcugcuaucuacugggu
guguaggugucugacaagaacgaccguauaguacgagaggaacuacgguu
gguggccacugguguaccgguuguucgagagagcacgugccggguagcca
cgccacacgggguaagagcugaacgcaucuaagcucgaaacccacuugga
aaagagacaccgccgaggucccgcguacaagacgcggucgauagacucgg
ggugugcgcgucgagguaacgagacguuaagcccacgagcacuaacagac
caa

..<<<<<<<<<<<.....((..((<<<<<<.[<<......<<<<<<.((.
.((.>>>.....<<<<<<...<<<))..)).>>>......>>>>>>...>
>>....<<<...>>>.....<<<<<.....>>>>>.>>...<<<<<<...
..<<....>>......>>>>>.>.........<<.....>>...<<<<<.
....<<.....>>....>>>>>...<<<...<<<...<<<<....>>>>.
....>>>....<<<<<<<<......<..<.....<<<<<<<......<<<
<......>>>>.((......<<<.....))>>>....>>>>>>>......
>..>...>>>>>>.>>......<<<<<<<........>>>>>>>......
<<<<<.<<<<<<>>>>>..>>>......]..>>>>>>.))..........
.....<<....>>....))..........<<<.....>>>..........
.........>>>>>>>>>>>.......<<<<<<<<<<....<.<<<<..<
<.........<<<<<....>>>>>.........>>..>>>>...>.>>>>
>>>>>>...<<<.......>>>.<<<<<<....<<<<<<<<<<<<<..<<
<...<<<<<...>>>>>...>>>...<<....>>....<<<<<...<<<<
..>>>>...>>>>><<<<<<....>>>>>>...>>>>>>>>>>>>>..<<
.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>
>>.....>>>....<<<..<<.<<........>>>>.>>>....>>>>>>
>>>...<<<.....>>>......>>>>>>......>.>>..<<<<<<...
.<<<<<....>>>>><.<<<<<<...<<<.<<<<<<<<<...<<<.....
<<..<<.>>..>>...>>.>...>>>>>>>>>>>>.......>>>>>>..
>..<<<<<<.<<<.....>>>.>>>>>>....<<<......>>>...<<<
<<<<...<<<(((.....<<<<<<.....<<...........<<<.<<<<
<<<<<<.......<<<<<.<<...<<<<.......>>>>...>>......
.<<<<......>>>>.>>>>>...>>>>>>>>>>>>>..........)))
>>....>>>>>>.>>>...>>>>>>><<<<<<<.......>>>>>..>>.
...>>>>>><...<<<<<<<<......<<<<<<<<....>>>>>>>>...
>>>>>>>>....>.....>>>>>><<.....<........<<<<<<....
...>>>>>><<<<<...<<<<..<<...<<<<<<...<<.....>>.>>>
>>>....((<<<<<<...<<<<<<.<<.....>>..>>>>>>.....>>>
..<<<<<........>>>>>))<<<<<<<<.......<<<...<<<<<<<
<...<<<<<<<<<<<<<.[[>>>>>>><...<<<<<....<<<<<....<
<<<...>>>>.....>>>>>.....>>>>>....>......>>>>>>...
>>>>>>>>.........>>>..>>>>>>>>.>>>..>>.....<<.....
.>>.>>>>..<<<.......>>>.>>>>>...<<<<.....<<<<<<<<.
<<.........<.....<<<<<...............>>>>><<<<<<<<
<.....>>>>>>>>>............<<<<<<<<<..<<<.........
>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<<
<..<<<<<<<..<<<.....<<<<......>>>>.....>>>>>>>>..>
>>>><<...>>........<...........<<<<<.......>>>>>..
...>....>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>>.>..
..>>.((.<<<<<<......))>>>>>>.<<<<<<<..(((.[[......
<<<<.[[<<<<..<<<<<.<<<<<<<<..<<....>>...<<<<....>>
>>.....>>>>>>>>..>>>>>...<<<.....>>>..<<<<<<......
.....>>>>>>..<<...<<<<<<<...<<<<<<..>>>>>>.....<<.
..(((>>....>>>>>>>...<<<<<<<<<.........>>>>>>>>>.<
<<....>>>.>>))).......<<<<<.<<<<<.>>>>>>>>>>>>>>>>
..........>>>>..]]>>>>.......<<<<.<.<<<<<<...<<...
....>>...>>>>>>...>>>>.>.......<<<<<..<<<<<..<<<<<
<<<<......>>>>>>>..>>..<<<<.....>>>>......>>>>>...
...>>>.>>....<<<<<<<....>>>.>>>>....]]..))).>>>>>>
>....<<<<<<...<<<<..<<<<<<<<...............>>>>>>>
><<<<<...<<<<...<<<<<<<<<<]]..>>>>>...>>>>>...>>>>
.>>>>>..<<<.<..<<<<<...........>>>>>...>>>>.>>>>..
.....>>>>>>.....<<<<<<<<<.......>>>>>....<<<.<<<<<
<<<....<<<<<..........>>>>>...>>>>.>>>>..>>>...>>>
>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2qa4 Structure of the base of the L7/L12 stalk of the Haloarcula marismortui large ribosomal subunit: Analysis of L11 movements
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): S1 K2 T3 N4 A12 K15 R19 A24 V25 D28 K35 P36 R37 R38 E42 N44 R47 R50 Y51 K63 L65 G66 S67 G68 S82 T84 S109 H110 V111 R112 V113 I114 R115
• Binding residue (residue number reindexed from 1): S1 K2 T3 N4 A12 K15 R19 A24 V25 D28 K35 P36 R37 R38 E42 N44 R47 R50 Y51 K63 L65 G66 S67 G68 S82 T84 S109 H110 V111 R112 V113 I114 R115

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0043232 intracellular non-membrane-bounded organelle
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:2qa4, PDBe:2qa4, PDBj:2qa4
• PDBsum: 2qa4
• PubMed: 17599351
• UniProt: P12733|RL18E_HALMA Large ribosomal subunit protein eL18 (Gene Name=rpl18e)