Structure of PDB 6i7o Chain Nb Binding Site BS01

Receptor Information

>6i7o Chain Nb (length=73) Species: 4932 (Saccharomyces cerevisiae)

RKKKVYTTPKKIKHKHKAVKLAVLSYYKVDAEGKVTKLRRECSNPTCGAG
VFLANHKDRLYCGKCHSVYKVNA

Ligand information

>6i7o Chain 2b (length=1758)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacaugguauaacu
gugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaaga
gauguauuuauuagauaaaaaaucaaugucuucggacucuuugaugauuc
auaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaa
auuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuu
caacggguaacggggaauaaggguucgauuccggagagggagccugagaa
acggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccu
aauucagggagguagugacaauaaauaacgauacagggcccauucggguc
uuguaauuggaaugaguacaauguaaauaccuuaacgaggaacaauugga
gggcaagucuggugccagcagccgcgguaauuccagcuccaauagcguau
auuaaaguuguugcaguuaaaaagcucguaguugaacuuugggcccgguu
ggccggucggauuuccaacggggccuuuccuucuggcuaaccuugagucc
uuguggcucuuggcgaaccaggacuuuuacuuugaaaaaauuagaguguu
caaagcaggcguauugcucgaauauauuagcauggaauaauagaauagga
cguuugguucuauuuuguugguuucuaggaccaucguaaugauuaauagg
gacggucgggggcaucaguauucaauugucagaggugaaauucuuggauu
uauugaagacuaacuacugcgaaagcauuugccaaggacguuuucauuaa
ucaagaacgaaaguuaggggaucgaagaugaucagauaccgucguagucu
uaaccauaaacuaugccgacuagggaucgggugguguuuuuuuaaugacc
cacucggcaccuuacgagaaaucaaagucuuuggguucuggggggaguau
ggucgcaaggcugaaacuuaaaggaauugacggaagggcaccaccaggag
uggagccugcggcuuaauuugacucaacacggggaaacucaccaggucca
gacacaauaaggauugacagauugagagcucuuucuugauuuugugggug
guggugcauggccguucuuaguugguggagugauuugucugcuuaauugc
gauaacgaacgagaccuuaaccuacuaaauaguggugcuagcauuugcug
guuauccacuucuuagagggacuaucgguuucaagccgauggaaguuuga
ggcaauaacaggucugugaugcccuuagacguucugggccgcacgcgcgc
uacacugacggagccagcgagucuaaccuuggccgagaggucuugguaau
cuugugaaacuccgucgugcuggggauagagcauuguaauuauugcucuu
caacgaggaauuccuaguaagcgcaagucaucagcuugcguugauuacgu
cccugcccuuuguacacaccgcccgucgcuaguaccgauugaauggcuua
gugaggccucaggaucugcagagcggagaauuuggacaaacuuggucauu
uagaggaacuaaaagucguaacaagguuuccguaggugaaccugcggaag
gaucauua

...<<<<<...[[[[>>>>>[[[[.((((((................[[[
.[[[..<<....<<....<<..........>>...>>.>>......{{..
......[[[..{{..{{....[[[..................<.....<<
.<<<.....>>>.>>......>.........<<<<<..<<....>>..>>
>>>[...((((((......<<<<<<<<<<<....>>>.............
.)))))).....]...<<<<..<<<.....>>>.>>>>....]]]...}}
}}..]]].<<<....<<<....<<<<<<<<.......>>>>>>>>>>>..
....>>>...<<<.<<<<....>>>>....>>>.}}.<<.<<<.......
...>>>.>>.<.<<....>>.>...]]]]]].........<<<....<<<
.....>>>..>>>...............<<<<<<<<<<..........>>
>>>>>.>>>......<<..<...........>..>>.........<<<<<
((......<<<<.....<<..))>>.......>>>>.>>>>>..))))))
]]]].........[[[{{.......{{...[[[.{{.....<<<<<.<<<
<<....<..>....>>>>>.>>>>>.....<<<<<<...........<..
.....>...........>>>>>>...<<<<<.<<.......<<...<...
....>..<<<.....>>>....>>......>>.>>..>>>.........[
[[..(((((((.....>>>>>>>>..))))))).]]].....}}....<<
<<<<.<<...<<<<..<<..<<<<<<.<...<<<......>>>......>
.>>>>>>..>>.......<<....>>...>>>>...>>>>>.>>>...]]
]...}}......<<<<<<<...<...<<<<.<.....>.>>>>...>>>>
>>>>..........<<<.<<.<<<..<<<<<<<<.<<<........>>>>
>>>>>>>..>>>...<<..}}>>...>>.....>>>.]]].<<<......
<<<<....>>>>....>>>..]]]]..<<<<<.<<<<<<<..<<.<<<<<
<..<<<.<<<<<.......<<........>>..........<<<<<.<..
..<<<<<........<<.<<<........>>>.>>......>>>>>...<
<.<<<..<<<<<<<<<....<<<.<<<<<....>>>...<<<......>>
>...>>.>>>....<<<<<.<<..<<<<..<<<<<.<<<<<<....>>>>
>>...>>>>>..>>>>.>>....<<<<<<.....>>>>>>.......>>>
>>....>>>.>>>...>.>>>>>>>.....>.>>>>>....>.>>>>.>>
>.....<<<<<<<......<<.....<<..<<<<....>>>>..>>....
>>.......>>>>>>>......<....<<<<<<..........>>>>>>.
...>.....>>>>>>....<<<<<<<<.......>>>>>>>>......>>
...>>>>>>>>>>.>>....<..<<.<..<<<<.<<....<<<<<<<<.<
<<..<.<<..<...<<<<<...>>>>>...>..>>.>..>>>.>>>>>>>
>...>>.>>>>...>.>>...>.....<<<<<<<<<....>>>>>>>>>.
........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6i7o Collided ribosomes form a unique structural interface to induce Hel2-driven quality control pathways.
• Resolution: 5.3 Å
• Binding residue (original residue number in PDB): R80 Y85 T87 P88 K89 I91 K92 H93 K94 H95 A97 L100 V102 R118 F131 L132 A133 N134 H135 R138 Y140 G142 K143 H145 S146 K149
• Binding residue (residue number reindexed from 1): R1 Y6 T8 P9 K10 I12 K13 H14 K15 H16 A18 L21 V23 R39 F52 L53 A54 N55 H56 R59 Y61 G63 K64 H66 S67 K70

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome

External links

• PDB: RCSB:6i7o, PDBe:6i7o, PDBj:6i7o
• PDBsum: 6i7o
• PubMed: 30609991
• UniProt: P05759|RS31_YEAST Ubiquitin-ribosomal protein eS31 fusion protein (Gene Name=RPS31)