Structure of PDB 8fku Chain NH Binding Site BS01

Receptor Information

>8fku Chain NH (length=180) Species: 9606 (Homo sapiens)

MRPLTEEETRVMFEKIAKYIGENLQLLVDRPDGTYCFRLHNDRVYYVSEK
IMKLAANISGDKLVSLGTCFGKFTKTHKFRLHVTALDYLAPYAKYKVWIK
PGAEQSFLYGNHVLKSGLGRITENTSQYQGVVVYSMADIPLGFGVAAKST
QDCRKVDPMAIVVFHQADIGEYVRHEETLT

Ligand information

>8fku Chain L3 (length=2185)

gcgaccucagaucagacguggcgacccgcugaaagcauauuagucagcgg
aggagaagaaacuaaccaggauucccucaguaacggcgagugaacaggga
agagcccagcgccgaauccccgccccgggggcgcgggacauguggcguac
ggaagacccgcuccccggcgccgcucguggggggcccaaguccuucugau
cgaggcccagcccguggacggugugaggccgguagcggcccccggcgcgc
cgggcccgggucuucccggagucggguugcuugggaaugcagcccaaagc
gggugguccaucuaaggcuaaauaccggcacgagaccgauagucaacaag
uaccguaagggaaaguugaaaagaacuuugaagagagaguucaagagggc
gugaaaccguuaagaggaacgggugggguccgcgcaguccgcccggagga
uucaacccggcggcggccgccgccgggcgcauuuccaccgcggcggugcg
ccgcgaccggcuccgggacggcugggaaggcccggcggggaagguggcuc
gggcccgaguguuacagcccccccggcagcagcacucgccgaaucccggg
gccgagggagcgccgcgcucuccggccgggccaggccacggagcgcacgg
ggucggcggcgacgucggcuacccacccgacccgucuugaaacacggacc
aaggagucuaacacgugcgcgagucgggggcucgcacgaaagccgccgug
gcgcaaggugggaucccgaggcccgccgagggcgcaccaccggcccgucu
cgcccggggagguggagcacgagcgcacguguuaggacccgaaagauggu
gaaaccaucuaguagcugguucccuccgaaguuucccggauagcuggcgc
ucuccgcaguuuuauccgguggguaagaagcccggcucgagccgggcgug
gaaugcgagugccuagugggccacuuuugguaagcagaacuggcgcugcg
ggaugaaccgaacgccggguuaaggcgcccgaugccgacgcucaucagac
cccagaaaagguguugguugauauagacagcaggacgguggccauggaag
ucggaauccgcuaaggaguguguaacaacucaccugccgaaucaacuagc
ccugaaaauggauggcgcuggagcgucgggcccauacccggccgucgccg
gcagucacgcuacgccgcgacgaguaggagggccgcugcggugagccuug
aagccuagggcgcgggcccggguggagccgccgcaggugcagaucuuggu
gguaguagcaggggcgauggccuccggcccucgcggagaugggcgccgcg
aggcguccagugaaucucgcauguagguaagggaaguacugucccuaccu
acuauccagcgaaaccacagccaagggaacgggcuuggcggaaucagcgg
ggagaagacccuguugagcuugacucuagucuggcacggugaagagacau
gagagguguagaauaagugggaggcggccgccggugaaauaccacuacuc
ugaucguuuuuuccagugccagguggggaguuauccuucugaccuuuugg
guuuuaagcaggaggugucagaaaaguuaccacagggauaacuggcuugu
ggcggccaagcgcgacgucgcuuuuugaugaugucggcucuuccuaucau
ugugaagcagaauucaccaagcguuggauuguucacccacuaauagggaa
cgugagcuggggucgugagaccccuacugaugguuguugccaugguaauc
cugcucaguacgagaggaaccgcagguucagacauuugguggugcuuggc
ugaggagccaauggggcgaagcuaccaucugugggauuaugacugaacgc
cucuaagucagaaucccgcccaggcggaacgauaagcgccgcggagccuc
gguuggccucggauagccgguccccgggguccggugcggagugcccuucg
uccugggaaacggggcgcggccggagaggcggccgcccccucgcccguca
cgcaccgcacguucguggggaaccuggcgcuaaaccauucguagacgacc
ugcuucugggucgggguuucguacguagcagagcagcucccucgcugcga
ucuauugaaagucagcccucgacacaaggguuugu

........................<<<<<<...............>>>>>
.>.........<<....>>..<<<<<<.....<<.....>>....>>>>>
>..<<<..<<<<<...<<<<<<<<<<.>>>>>>.>>>>.....>>>>>..
<<<<<<<<<<...<<<<<<<<.<<.....<<<<<<<<....<<<<.....
.>>>>.....((......<<<.....))>>>...>.>>>>>>>.>>>>>>
>>>>..>>>>>>>>>>.......<<<<<<<........>>>>>>>.....
<<<<<<.>>>>>>..>>>................................
..<<....>>...............<<<<....>>>>.............
...................<<<<<<<<..<<<.<<...<<<<<<<<.<<<
.....<<<<<<<<<<..>>>>>>>>>>......>>>.>>>.>>>>>...<
<<<<<<.<<<<<<<<<<<<<<<...<<..<<<.<<.<<<<......<<<<
<<..>>>>>>.......>>>>>>.>>>........>>>>>>..>>>>>>>
>>>>..........<<<<<<.<..>.<<<<<<.>>>>.>>>>>>>>....
..>>.>>>>>...>>>>>...>>>>>>>>..<<<<.<....>.>>>><<<
<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>.....>
>>....<<<<<...<<<..<<<..>>>..>>>....>>>>>.<<<<<<<<
<.<<..>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.<...<<<<<<
...>>>>>>......>.>>..<<<<<<....<.<<<.>>>.><<.<<<<<
<<...<<<.<<<<<<<<...>>>>>>...<<<<<<<<..>>>>>>>>...
.>>>>>>>>>>>>..>>..<<<<.<<<<<.....>>>>>.>>>>....<<
<......>>>...<<<<<<<...<<<(((.......<<<<<.....<<..
........<<<.<<<<<<<<<<.....<.<<<<<.<<....<<.......
..>>....>>.........<<......>>...>>>>>.>.>>>>>>>>>>
>>>..........))).>>...>>>>>..>>>...>>>>>>><<<<<<.<
<<...........>>>>>>>>>....>>>>>>.<.<<<<<<<<.<<....
..<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>>>>
<<<....<...<<<<...........>>>>..<<<<<...<<<<<<<...
.>>>>>>>......>>.>>>..<<<<<<.<<<<<<<..>>.>>>>>>>>>
>>.......>...>>>...<<<<<<........>>>>>>....<<<<.<<
<<......<<<<<<.<<<<<<.<<<<<.<<<<<<<<<...<<<<<<<<<<
.<<<<..........<<<<<..<<<.>>>............>>>>>.>>>
>.>>.>>>>>>>>..>>>>>>>>>..>>>>>...<<<<<<<..<<<....
.....>>>>>>>>>>........>>>>>.>.>>>>>>.............
<<<<<<...<..>...>>>>>>.......((...<<<<<<<<<<<..<.<
.<<<<<......>>>>>...>.>.<<<.....))..>>>.....>>>>>.
...>>>>>>..<<<....>>>>>>>.>>>>..<<<<<<.<..<<<..<.<
<<<<.................>>>>>.><<<<....<<<<.<<.<<<<<<
<....>>>>>..>>.>>...>>>>...>>>><<<<<...<<<<<......
.....>>>>>...>>>>>.>>>...>.>>>>>>.<<<<<<<.<<..<<<<
....<<<........<<<<<.<<<<>>>>.>>>>>.......>>>...<<
<..<<<<....<<<<.<<<<<<<......>>>>>>>>>>>...>>>>..>
>>..............>>>>..>>>>>>>>><<<<<.<............
.>....<<.<<<<<<<.<<.....<<<<....<<<<<......>>>>>..
.>>>>....>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fku Principles of human pre-60 S biogenesis.
• Resolution: 2.82 Å
• Binding residue (original residue number in PDB): M1 K15 H40 N41 A56 N57 K72 T74 R80 L81 Y128 F164 Q166
• Binding residue (residue number reindexed from 1): M1 K15 H40 N41 A56 N57 K72 T74 R80 L81 Y128 F164 Q166

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0005515 protein binding

Biological Process

• GO:0042254 ribosome biogenesis
• GO:0042255 ribosome assembly
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005829 cytosol
• GO:0030687 preribosome, large subunit precursor

External links

• PDB: RCSB:8fku, PDBe:8fku, PDBj:8fku
• PDBsum: 8fku
• PubMed: 37410842
• UniProt: Q9Y221|NIP7_HUMAN 60S ribosome subunit biogenesis protein NIP7 homolog (Gene Name=NIP7)