Structure of PDB 8fkt Chain NH Binding Site BS01

Receptor Information

>8fkt Chain NH (length=180) Species: 9606 (Homo sapiens)

MRPLTEEETRVMFEKIAKYIGENLQLLVDRPDGTYCFRLHNDRVYYVSEK
IMKLAANISGDKLVSLGTCFGKFTKTHKFRLHVTALDYLAPYAKYKVWIK
PGAEQSFLYGNHVLKSGLGRITENTSQYQGVVVYSMADIPLGFGVAAKST
QDCRKVDPMAIVVFHQADIGEYVRHEETLT

Ligand information

>8fkt Chain L3 (length=2125)

gcgaccucagaucagacguggcgacccgcugaaagcauauuagucagcgg
aggagaagaaacuaaccaggauucccucaguaacggcgagugaacaggga
agagcccagcgccgaauccccgccccgggggcgcgggacauguggcguac
ggaagacccgcuccccggcgccgcucguggggggcccaaguccuucugau
cgaggcccagcccguggacggugugaggccgguagcggcccccggcgcgc
cgggcccgggucuucccggagucggguugcuugggaaugcagcccaaagc
gggugguccaucuaaggcuaaauaccggcacgagaccgauagucaacaag
uaccguaagggaaaguugaaaagaacuuugaagagagaguucaagagggc
gugaaaccguuaagaggaacgggugggguccgcgcaguccgcccggagga
uucaacccggcggcgccgggcgcauuuccaccgcggcggugcgccgcgac
cggcuccgggacggcugggaaggcccggcggggaagguggcucgggcccg
aguguuacagcccccccggcagcagcacucgccgaaucccggggccgagg
ggucggcggcgacgucggcuacccacccgacccgucuugaaacacggacc
aaggagucuaacacgugcgcgagucgggggcucgcacgaaagccgccgug
gcgcaaggugggaucccgaggcccaguccgccgagggcgcaccaccggcc
gcacgagcgcacguguuaggacccgaaagauggugaaaccaucuaguagc
ugguucccuccgaaguuucccggauagcuggcgcucuccgcaguuuuauc
cgguggguaagaagccggcguggaaugcgagugccuagugggccacuuuu
gguaagcagaacuggcgcugcgggaugaaccgaacgccggguuaaggcgc
ccgaugccgacgcucaucagaccccagaaaagguguugguugauauagac
agcaggacgguggccauggaagucggaauccgcuaaggaguguguaacaa
cucaccugccgaaucaacuagcccugaaaauggauggcgcuggagcgucg
ggcccauacccggccgucgccggcagucgagaguggacggccgcggacgc
uacgccgcgacgaguaggagggccgcugcggugagccuugaagccuaggg
cgcgggcccggguggagccgccgcaggugcagaucuuggugguaguagca
ggggcgauggccuccggcccucgcggagaugggcgccgcgaggcguccag
ugaaucucgcauguagguaagggaaguacugucccuaccuacuauccagc
gaaaccacagccaagggaacgggcuuggcggaaucagcggggagaagacc
cuguugagcuugacucuagucuggcacggugaagagacaugagaggugua
gaauaagugggaggcggccgccggugaaauaccacuacucugaucguuuu
uuccagugccagguggggaguuauccuucugaccuuuuggguuuuaagca
ggaggugucagaaaaguuaccacagggauaacuggcuuguggcggccaag
cgcgacgucgcuuuuugaugaugucggcucuuccuaucauugugaagcag
aauucaccaagcguuggauuguucacccacuaauagggaacgugagcugg
ggucgugagaccccuacugaugguuguugccaugguaauccugcucagua
cgagaggaaccgcagguucagacauuugguggugcuuggcugaggagcca
auggggcgaagcuaccaucugugggauuaugacugaacgccucuaaguca
gaaucccgcccaggcggaacgauaagcgccgcggagccucgguuggccuc
ggauagccgguccccgggguccggugcggagugcccuucguccugggaaa
cggggcgcggccggagaggcggccgcccccucgcccgucacgcaccgcac
guucguggggaaccuggcgcuaaaccauucguagacgaccugcuucuggg
ucgggguuucguacguagcagagcagcucccucgcugcgaucuauugaaa
gucagcccucgacacaaggguuugu

........................<<<<<<...............>>>>>
.>.........<<....>>..<<<<<<.....<<.....>>....>>>>>
>..<<<..<.<<<...<<<<<<<<<<.>>>>>>.>>>>.....>>>.>..
<<<<<<<<<<...<<<<<<<<.<<.....<<<<<<<<....<<<<.....
.>>>>.....((......<<<.....))>>>...>.>>>>>>>.>>>>>>
>>>>..>>>>>>>>>>.......<<<<<<<........>>>>>>>.....
<<<<<<.>>>>>>..>>>................................
..<<....>>...............<<<<....>>>>.............
...................<<<<<<<<..<<<......<<<<<<<<.<<<
.....<<<<<<<.>>>>>>>......>>>.>>>.>>>>>..<<<<<<<<.
<<<<<<<<<<<<<<<...<<..<<<.<<.<<<<......<<<<<<..>>>
>>>.......>>>>>>.>>>........>>>>>>..>>>>>>>>>>>...
..>>.>>>>>>....>>>...>>>>>>>>..<<<<.<....>.>>>><<<
<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>.....>
>>....<<<<<...<<<..<<.........>>..>>>....>>>>>.<<.
>>....>>>>>>>>>>>>>..<<.<...<<<<<<...>>>>>>......>
.>>..<<<<<<....<.<<<.>>>.><<.<<<<<<<...<<<.<<<<<<<
<...>>>>>>...<<..>>....>>>>>>>>>>>>..>>..<<<<.<<<<
<.....>>>>>.>>>>....<<<......>>>...<<<<<<<...<<<((
(.......<<<<<.....<<..........<<<.<<<<<<<<<<.....<
.<<<<<.<<....<<.........>>....>>.........<<......>
>...>>>>>.>.>>>>>>>>>>>>>..........))).>>...>>>>>.
.>>>...>>>>>>><<<<<<.<<<.............<<..>>.......
...>>>>>>>>>....>>>>>>...<<<<<<<<.<<......<<<<.<<<
<....>>>>>>>>...>>>>>>>>>>........>>>>>><<.....<..
.<<<<...........>>>>..<<<<<...<<<<<<<....>>>>>>>..
....>>.>>>..<<<<<<.<<<<<<<..>>.>>>>>>>>>>>.......>
....>>...<<<<<<........>>>>>>....<<<<.<<<<......<<
<<<<.<<<<<<.<<<<<.<<<<<<<<<...<<<<<<<<<<.<<<<.....
.....<<<<<..<<<.>>>............>>>>>.>>>>.>>.>>>>>
>>>..>>>>>>>>>..>>>>>...<<<<<<<..<<<.........>>>>>
>>>>>........>>>>>.>.>>>>>>.............<<<<<<...<
..>...>>>>>>.......((...<<<<<<<<<<<..<.<.<<<<<....
..>>>>>...>.>.<<<.....))..>>>.....>>>>>....>>>>>>.
.<<<....>>>>>>>.>>>>..<<<<<<.<..<<<..<.<<<<<......
...........>>>>>.><<<<....<<<<.<<.<<<<<<<....>>>>>
..>>.>>...>>>>...>>>><<<<<...<<<<<...........>>>>>
...>>>>>.>>>...>.>>>>>>.<<<<<<<.<<..<<<<....<<<...
.....<<<<<.<<<<>>>>.>>>>>.......>>>...<<<..<<<<...
.<<<<.<<<<<<<......>>>>>>>>>>>...>>>>..>>>........
......>>>>..>>>>>>>>><<<<<.<.............>....<<.<
<<<<<<.<<.....<<<<....<<<<<......>>>>>...>>>>....>
>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fkt Principles of human pre-60 S biogenesis.
• Resolution: 2.81 Å
• Binding residue (original residue number in PDB): M1 K15 H40 N41 A56 N57 S59 K72 T74 R80 L81 Y128 F164 Q166
• Binding residue (residue number reindexed from 1): M1 K15 H40 N41 A56 N57 S59 K72 T74 R80 L81 Y128 F164 Q166

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0005515 protein binding

Biological Process

• GO:0042254 ribosome biogenesis
• GO:0042255 ribosome assembly
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005829 cytosol
• GO:0030687 preribosome, large subunit precursor

External links

• PDB: RCSB:8fkt, PDBe:8fkt, PDBj:8fkt
• PDBsum: 8fkt
• PubMed: 37410842
• UniProt: Q9Y221|NIP7_HUMAN 60S ribosome subunit biogenesis protein NIP7 homolog (Gene Name=NIP7)