Structure of PDB 5wlc Chain NG Binding Site BS01

Receptor Information

>5wlc Chain NG (length=111) Species: 1247190 (Saccharomyces cerevisiae BY4741)

NSQVFGVARIYASFNDTFVHVTDLSGKETIARVTGGMKVKADRDESSPYA
AMLAAQDVAAKCKEVGITAVHVKIRATGGTRTKTPGPGGQAALRALARSG
LRIGRIEDVTP

Ligand information

>5wlc Chain L1 (length=1025)

agauaguuaucugguugauccugccaguagucauaugcuugucucagcca
ugcagcucauuaaaucaguuaucguuuauuugauagucaugguauaacug
ugguaauucuagagcuaaaaucucgacccuuuggaagagauguauuuauu
agaucaaugucuucggacucuuugaugaauaacuuuucgaaucgcauggc
cuugugcuggcgaugguucauucaaauuucugcccuaucaacuuucgaug
guaggauaguggccuaccaugguuucaacggguaacggggaauaaggguu
cgauuccggagagggagccugagaaacggcuaccacauccaaggaagggc
aggcuuacccaauccuaauucagggagguagugacaauaaauaacgauac
agggcccauucgggucuuguaauuggaaugaguacaauguaaauaccuua
acgaggaacaauuggagggcaagucuggugccagcagccgcgguaauucc
agcuccaauagcguauauuagcucguaguugaacuauuaauagggacggu
cgggggcaucaguauucaauugucagaggugaaauucuuggauuuauuga
agacuaacuacugcgaaagcauuugccaaggacguuuucauuaaucaaga
acgaaaguuaggggaucgaagaugaucagauaccgucguagucuuaagga
guauggucgcaaggcugaaacuuaaaggaauugacggaagggcaccacca
ggaguggagccugcggcucaacacggggaaacucaccagguccagacaca
auauuugugggugguggugcaugugaugcccuuguucuggcgcgcgcuac
acugacggagccagcgagucuaaccuuggccgagaggucuugguaaaaac
uccgucggggaacgaggaauuccuaguaagcgcaagucaucagcuugcgu
ugauuacgucccugcccuuuguacacaccgcccgucgcuaguaccggaac
uaauuccguaggugaaccugcggaa

.............................<<.<<<<<<........<<<.
<<<..<<<.......<<<..<<..<<....<<<..............<<.
<<<.....>>>.>>.....<<<<<..<<....>>..>>>>>....<<<<.
...<<<<.<<<....>>>...>>>>...>>>>.........<<<<..<<<
.....>>>.>>>>....>>>...>>>>..>>>.<<<....<<.....<<<
<<<<<.......>>>>>>>>.>>......>>>....<<<...<.......
.>..>>>.>>>.<<.<<<..........>>>.>>.<.<<....>>.>.>>
>>>>..<<<....<<<.....>>>..>>>...............<<<<<<
<.<<<<<....>>>>>.>>>.>>>>......<<..<..<....>...>..
>>.........<<<<<<.......<<<....>>>................
..>>>>>>..>>>>>>>>..<.<<...<<<.<<...>>....<<<<<<.<
<...<<<<..<<<.<<<<<<.<...<<<......>>>......>.>>>>>
>.>>>......<<<....>>>..>>>>..>>.>>>.>>>...>>>...>>
.>.....<<<<<<...<...<<<<.........>>>>...>>>>>>>...
..............<<.........>>.....<<<<<<<<<<<<..<<.<
<<<<<..<<<.<<<<....................<<<<<......<<<<
<...>>>>>......<<<<<<..>>.>>>>.....>>>>>>>>>.>>>..
...<<<<<<<............<<<..<<<<....>>>>..>>>.....>
>>>>>>............>>>>>>....<<<<<<<.........>>>>>>
>......>>...>>>>>>>............>>>>>..<<<<.<<>>.>>
>>.<<<<<<<<<....>>>>>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5wlc The complete structure of the small-subunit processome.
• Resolution: 3.8 Å
• Binding residue (original residue number in PDB): N24 D25 H29 G35 G45 A50 D51 G88 P120
• Binding residue (residue number reindexed from 1): N15 D16 H20 G26 G36 A41 D42 G79 P111

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0030686 90S preribosome
• GO:0032040 small-subunit processome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5wlc, PDBe:5wlc, PDBj:5wlc
• PDBsum: 5wlc
• PubMed: 28945246
• UniProt: P06367|RS14A_YEAST Small ribosomal subunit protein uS11A (Gene Name=RPS14A)