Structure of PDB 8fl2 Chain NB Binding Site BS01

Receptor Information

>8fl2 Chain NB (length=81) Species: 9606 (Homo sapiens)

MKRPKLKKASKRMTCHKRYKIQKKVREHHRKLRKEAKKRGHKKPRKDPGV
PNSAPFKEALLREAELRKQRLEELKQQQKLD

Ligand information

>8fl2 Chain L3 (length=3495)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccggggcgcgggacauguggc
guacggaagacccgcuccccggcgccgcgggggcccaaguccuucugauc
gaggcccagcccguggacggugugaggccgguagcggcccccggcgcgcc
gggcccgggucuucccggagucggguugcuugggaaugcagcccaaagcg
ggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauagucaa
caaguaccguaagggaaaguugaaaagaacuuugaagagagaguucaaga
gggcgugaaaccguuaagagguaaacgggugggguccgcgcaguccgccc
ggaggauucaacccggcggcggccggccgugucggcggcccggcggaucu
gggaccgucccccgaccggcgaccggccgccgccgggcgcauuuccaccg
cggcggugcgccgcgaccggcuccgggacggcugggaaggcccggcgggg
aagguggcucgggcccgaguguuacagcccccccggcagcagcacucgcc
gaaucccggggccgagggagucgccgcgcucuccccccggggggccgggc
caccccucccacggcgcgcgccgucgggcccggggggccacgcgcgcguc
ccccgaagagggggacggcggagcgagcgcacggggucggcggcgacguc
ggcuacccacccgacccgucuugaaacacggaccaaggagucuaacacgu
gcgcgagucgggggcucgcacgaaagccgccguggcgcaaugaaggugaa
ggccgcggccgaggugggaucccgaggcccgccgagggcgcaccaccggc
ccgucucgcccgccgcgccggggagguggagcacgagcgcacguguuagg
acccgaaagauggugaacuaugccugggcagggcgaagccagaggaaacu
cugguggagguccguagcgguccugacgugcaaaucggucguccgaccug
gguauaggggcgaaagacuaaucgaaccaucuaguagcugguucccuccg
aaguuucccucaggauagcuggcgcucuccgcaguuuuauccgguaaagc
gaaugauuagaggucuuggggccggaucucaaccuauucucggguaagaa
gcccggcucgcuggcguggagccgggcguggaaugcgagugccuaguggg
ccacuuuugguaagcagaacuggcgcugcgggaugaaccgaacgccgggu
uaaggcgcccgaugccgacgcucaucagaccccagaaaagguguugguug
auauagacagcaggacgguggccauggaagucggaauccgcuaaggagug
uguaacaacucaccugccgaaucaacuagcccugaaaauggauggcgcug
gagcgucgggcccauacccggccgucgccggcagugacgcuacgccgcga
cgaguaggagggccgcugcggugagccuugaagccuagggcgcgggcccg
gguggagccgccgcaggugcagaucuuggugguaguagcaaauauucaaa
cgagaacuuugaaggccgaaguggagaaggguuccaugugaacagcaguu
gaacaugggucagucgguccugagagaugggcgagcgccguuccgaaggg
acgggcgauggccuccggcccucgaucgaaagggagucggguucagaucc
ccgaauccggaguggcggagaugggcgccgcgaggcguccagugcgguaa
cgcgaccgaucccggagaagccggcgggagccccggggagaguucucuuu
ucuuugugaagggcagggcgcccuggaauggguucgccccgagagagggg
cccgugccuuggaaagcgucgcgguuccggcggcguccggugagcucucg
cuggcccuugaaaauccgggggagaggguguaaaucucgcgccgggccgu
acccauauccgcagcaggucuccaaggugaacagccucuggcauguugga
acaauguagguaagggaagucggcaagccggauccguaacuucgggauaa
ggauuggcucuaagggcugggucggucgggcuggggcgcgaagcgggguc
ccguggaucgccccagccggccggcgccuagcagccgacuuagaacuggu
gcggaccaggggaauccgacuguuuaauuaaaacaaagcaucgcgaaggc
ccgggguguugacgcgaugugauuucugcccagugcucugaaugucaaag
ugaagaaauucaaugaagcgcggguaaacggcgggaguaacuaugacucu
cuuaagguagccaaaugccucgucaugugacgcgcaugaauggaugaacg
agauucccacugucccuaccuacuauccagcgaaaccacagccaagggaa
cgggcuuggcggaaucagcggggaaagaagacccuguugagcuugacucu
agucuggcacggugaagagacaugagagguguagaauaagugggaggcgg
ccgccggugaaauaccacuacucugaucguuuuuucacugacccggugag
gcggggggcuucuggcgccaagcgcccggccgcgcgccggccgggcgcga
cccgcuccggggacagugccagguggggaguuugguacaccugucaaacg
guaacgcagguguccuaaggcgagcucagggaggacagaaaccucccgug
gagcagaagggcaaaagcucgcuugaucuugauuuuguacgaauacagac
cgugaaagcggggccucacccuucugaccuuuuggguuuuaagcaggagg
aaguuaccacagggauaacuggcuuguggcggccaagcgagcgacgucgc
uuuuugauccuucgaugucggcucuuccuaucauugugaagcagaauuca
ccaagcguuggauuguucacccacuaauagggaacgugagcuggguuuag
accgucgugagacagguuaguuuuacccuacugaugauguguuguugcca
ugguaauccugcucaguacgagaggaaccgcagguucagacauuuggugu
augugcuuggcugaggagccaauggggcgaagcuaccaucugugggauua
ugacugaacgccucuaagucagaaucccgcccaggcggaacgauagcgcc
gcggagccucgguuggccucggauagccgguccccgggguccggugcgga
gugcccuucguccugggaaacggggcgcggccggagaggcggccgccccc
ucgcccgucacgcaccgcacguucguggggaaccuggcgcuaaaccauuc
guagacgaccugcuucugggucgggguuucguacguagcagagcagcucc
cucgcugcgaucuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<.....<<....>>......
>>>>>.>.........<<....>>..<<<<<<.....<<.....>>....
>>>>>>..<<<..<<<<<...<<<<<<<<<..>>>>>.>>>>.....>>>
>>..<<<<<<<<<<...<<<<<<<<.<<<<<<<<<<....<<<<......
>>>>.....((......<<<.....))>>>...>.>>>>>>>.>>>>>>>
>>>..>>>>>>>>>>.......<<<<<<<........>>>>>>>.....<
<<<<..((((((>>>>>..>>>............................
......<<....>>...............<<<......>>>.........
.........................<<<<<<<<..<<<.<<...<<<<<<
<<.<<<.....<<<<<<<<<<<<<<<<<<.<<<<<.<<...<<<<<.<<<
.>>>>>>>>>>>>>>>>>>>...>>>>>>>>>>>>>>......>>>.>>>
.>>>>>...<<<<<<<<<<<<<<<<<<<<<<<...<<..<<<.<<.<<<<
......<<<<<<..>>>>>>.......>>>>>>.>>>........>>>>>
>..>>>>>>>>>>>...........<<<<<<.<<<<<..>>>>>.<<<<<
<.<<<<.<<<<<<<<<..>>>>>>.>>>...>>>>>>>>.>>..<<<<<<
<<<.......>>>>>>>.>>....>>>>>>.....>>>.>>>>>...>>>
>>...>>>>>>>>..<<<<........>>>><<<<<<....<<<<<<<<<
<<<<..<<<...<<<<........>>>>.....>>>...<<....>>...
<<<<..>>>>..<<<<<...<<<..<<<..>>>..>>>....>>>>>.<<
<<<<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>.
.<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>
>>>>>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>
>>>>>...<<.......>>......>>>>>>......>.>>..<<<<<<.
.....<<<....>>>..<<<<<<<<<<...<<<.<<<<<<<<........
....<<....<<<..<<<<<<.<.>.>>>>>>>>>..>>..>>>>>>...
<<<<<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>>.>>>..<
<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<
...<<<(((.......<<<<<.....<<..........<<<.<<<<<<<<
<<.......<<<<<.<<<...<<.........>>...>>>.........<
<......>>...>>>>>...>>>>>>>>>>>>>..........))).>>.
..>>>>>..>>>...>>>>>>><<<<<<.<<<...........>>>>>>>
>>....>>>>>>.<.<<<<<<<<.<<......<<<<.<<<<....>>>>>
>>>...>>>>>>>>>>..>.....>>>>>><<.....<<.....<<<<<<
.......>>>>>>.<<<.....<<<<<.<<...<<<<<....<<.....>
>..>>>>>....((<<<<<<...<<....<<<....>>>...........
..<<<<...........>>>>....>>....>>>..<<<<<........>
>>>>))<<<<....<<<<<..<<<<<<<<....>>>>>>>>..<<<....
>>>...<..<<<<<<<...<<<<<<<<<<<<.<<<<<<<<<<..[[[..>
>>>>>....<<<<<.<<<<.<<<<.....<<<.....>>>.....>>>>>
>>>..>>>>>.....<<<<<<<.......>>>>>>>>>>>...>>>>>>>
>>>>>.........>>>>>>>..>..........>>.>>>.>>>>.>>>.
.>>.....<<......>>>>>>>.<<<<.....>>>>..>>>....<<<<
.....<<<<<<<.<<...............<<<<<.....<..>......
>>>>><<.<<<<<<<<<<<.<<<<<<<<.<<<<<<<<<....<<<<<..>
>>>>....>>>>>>>>>>>>>>>>>......>>>>>..>>>>>>.>>...
.........<<<<<<<<<..<<<.........>>>..<<<<<<<....<<
<<.>>>>......>>>>>>>.......<<<..<<<<<<....<<<.....
<<<<....>>>>.....>>>.>>>>..>>>>><<<<<<.......>>>>>
>.............<<<<..<<<<<<>>>>>>.>>>>...........>>
.>>>>>>>.....>>.>>>>>>>..>>>>>>....>>....<<<<<....
....>>>>>....<<<<<..<<<........<<<<<.<<<<<<..<<<<<
.<<<<<<<<<.<<<<<<<<<<<<.<<<<..........<<<<<..<<<.>
>>............>>>>>.>>>>.>>.>>>>>>>>>>...<<<<<....
.<<<<................<<<<<<<<<<.......>>>>>>>>>>..
>>>>..>>>>>....>>>>>>>>>..>>>>>..<<.<<<<<<<.......
......>>>>>>>>>...<<<<<<<...<<<<<<.......>>>>>>...
..<<...(((>>....>>>>>>>...<<<..<<<<.[[..>>>>..>>>.
<<<....>>>...))).]]<<<<<<<..<<<..)))))).>>>>>>>>>>
.>>>>..>>>>>>>.............<<<<<<...<<..>>...>>>>>
>.................<<<<.<<<<<<..<.<.<<<<<......>>>>
>.>...>.<<<.........>>>.....>>>>>..>...>>>>......<
<<<<<<....>>>.>>>>........>>>.>>>>>.....<<<<<<.<..
<<<..<.<<<<<.................>>>>>.><<<<....<<<<..
..<<.<<<<<<<]]].>>>>>..>>.>>...>>>>...>>>><<<<<...
<<<<<...........>>>>>...>>>>>.>>>...>.>>>>>>.<<<<<
<.<<..<<<<....<<<........<<<<<.<<<<>>>>.>>>>>.....
..>>>...<<<..<<<<....<<<<.<<<<<<<......>>>>>>>>>>>
...>>>>..>>>..............>>>>..>>>>>>>>.<<<<<.<..
...........>....<<.<<<<<<<.<<.....<<<<.<..<<<<<...
...>>>>>..>>>>>....>>....>>>.>>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fl2 Principles of human pre-60 S biogenesis.
• Resolution: 2.67 Å
• Binding residue (original residue number in PDB): M1 K2 R3 P4 K7 S10 K11 R12 M13 T14 C15 H16 K17 R18 Y19 I21 K24 V25 R26 H28 P51 N52
• Binding residue (residue number reindexed from 1): M1 K2 R3 P4 K7 S10 K11 R12 M13 T14 C15 H16 K17 R18 Y19 I21 K24 V25 R26 H28 P51 N52

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0005515 protein binding
• GO:0005525 GTP binding
• GO:0048027 mRNA 5'-UTR binding

Biological Process

• GO:0017145 stem cell division
• GO:0019827 stem cell population maintenance
• GO:0032206 positive regulation of telomere maintenance
• GO:0033235 positive regulation of protein sumoylation
• GO:0042127 regulation of cell population proliferation
• GO:1902895 positive regulation of miRNA transcription
• GO:1904816 positive regulation of protein localization to chromosome, telomeric region

Cellular Component

• GO:0005615 extracellular space
• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005694 chromosome
• GO:0005730 nucleolus
• GO:0016020 membrane
• GO:0016604 nuclear body
• GO:0030496 midbody

External links

• PDB: RCSB:8fl2, PDBe:8fl2, PDBj:8fl2
• PDBsum: 8fl2
• PubMed: 37410842
• UniProt: Q9BVP2|GNL3_HUMAN Guanine nucleotide-binding protein-like 3 (Gene Name=GNL3)