Structure of PDB 5wlc Chain NA Binding Site BS01

Receptor Information

>5wlc Chain NA (length=207) Species: 1247190 (Saccharomyces cerevisiae BY4741)

KNLSSFEKQQIEIRKQIEQLENEAVAEKKWSLKGEVKAKDRPEDALLTEE
LEFDRTAKPVPVITSEVTESLEDMIRRRIQDSNFDDLQRRTLALSEELQK
AHSEISELYANLVYKLDVLSSVHFVPKPASTSLEIRVETPTISMEDAQPL
YMSNASSLAPQEIYNVGKAEKDGEIRLKNGVAMSKEELTREDKNRLRRAL
KRKRSKA

Ligand information

>5wlc Chain L1 (length=1025)

agauaguuaucugguugauccugccaguagucauaugcuugucucagcca
ugcagcucauuaaaucaguuaucguuuauuugauagucaugguauaacug
ugguaauucuagagcuaaaaucucgacccuuuggaagagauguauuuauu
agaucaaugucuucggacucuuugaugaauaacuuuucgaaucgcauggc
cuugugcuggcgaugguucauucaaauuucugcccuaucaacuuucgaug
guaggauaguggccuaccaugguuucaacggguaacggggaauaaggguu
cgauuccggagagggagccugagaaacggcuaccacauccaaggaagggc
aggcuuacccaauccuaauucagggagguagugacaauaaauaacgauac
agggcccauucgggucuuguaauuggaaugaguacaauguaaauaccuua
acgaggaacaauuggagggcaagucuggugccagcagccgcgguaauucc
agcuccaauagcguauauuagcucguaguugaacuauuaauagggacggu
cgggggcaucaguauucaauugucagaggugaaauucuuggauuuauuga
agacuaacuacugcgaaagcauuugccaaggacguuuucauuaaucaaga
acgaaaguuaggggaucgaagaugaucagauaccgucguagucuuaagga
guauggucgcaaggcugaaacuuaaaggaauugacggaagggcaccacca
ggaguggagccugcggcucaacacggggaaacucaccagguccagacaca
auauuugugggugguggugcaugugaugcccuuguucuggcgcgcgcuac
acugacggagccagcgagucuaaccuuggccgagaggucuugguaaaaac
uccgucggggaacgaggaauuccuaguaagcgcaagucaucagcuugcgu
ugauuacgucccugcccuuuguacacaccgcccgucgcuaguaccggaac
uaauuccguaggugaaccugcggaa

.............................<<.<<<<<<........<<<.
<<<..<<<.......<<<..<<..<<....<<<..............<<.
<<<.....>>>.>>.....<<<<<..<<....>>..>>>>>....<<<<.
...<<<<.<<<....>>>...>>>>...>>>>.........<<<<..<<<
.....>>>.>>>>....>>>...>>>>..>>>.<<<....<<.....<<<
<<<<<.......>>>>>>>>.>>......>>>....<<<...<.......
.>..>>>.>>>.<<.<<<..........>>>.>>.<.<<....>>.>.>>
>>>>..<<<....<<<.....>>>..>>>...............<<<<<<
<.<<<<<....>>>>>.>>>.>>>>......<<..<..<....>...>..
>>.........<<<<<<.......<<<....>>>................
..>>>>>>..>>>>>>>>..<.<<...<<<.<<...>>....<<<<<<.<
<...<<<<..<<<.<<<<<<.<...<<<......>>>......>.>>>>>
>.>>>......<<<....>>>..>>>>..>>.>>>.>>>...>>>...>>
.>.....<<<<<<...<...<<<<.........>>>>...>>>>>>>...
..............<<.........>>.....<<<<<<<<<<<<..<<.<
<<<<<..<<<.<<<<....................<<<<<......<<<<
<...>>>>>......<<<<<<..>>.>>>>.....>>>>>>>>>.>>>..
...<<<<<<<............<<<..<<<<....>>>>..>>>.....>
>>>>>>............>>>>>>....<<<<<<<.........>>>>>>
>......>>...>>>>>>>............>>>>>..<<<<.<<>>.>>
>>.<<<<<<<<<....>>>>>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5wlc The complete structure of the small-subunit processome.
• Resolution: 3.8 Å
• Binding residue (original residue number in PDB): K322 A491 L509 K510 N511 R522 K525 R527 R529 R530 R534 S537 K538
• Binding residue (residue number reindexed from 1): K28 A159 L177 K178 N179 R190 K193 R195 R197 R198 R202 S205 K206

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0005515 protein binding
• GO:0042802 identical protein binding

Biological Process

• GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005732 sno(s)RNA-containing ribonucleoprotein complex
• GO:0030686 90S preribosome
• GO:0032040 small-subunit processome
• GO:0034457 Mpp10 complex
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5wlc, PDBe:5wlc, PDBj:5wlc
• PDBsum: 5wlc
• PubMed: 28945246
• UniProt: P47083|MPP10_YEAST U3 small nucleolar RNA-associated protein MPP10 (Gene Name=MPP10)