Structure of PDB 8v9l Chain N Binding Site BS01

Receptor Information

>8v9l Chain N (length=145) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

VIKLHDLKPAPGEKKAKTRVGRGEGSKGKTAGRGTKGTKARKNVPVMFEG
GQMPIHMRLPKLKGFKNRFRTEYQVVNVGDINKAFPQGGTVGVDELVAKG
LVRKNSLVKVLGDGKLTVKVDVTANKFSGSAREAITAAGGSATEL

Ligand information

>8v9l Chain A (length=3073)

aaguguuuaagggcgcaugguggaugccuuggcacugggagccgaugaag
gacguaggaggcugcgauaagccucggggagcugucaaccgagcguugau
ccgaggauguccgaauggggaaacccggcacgagugaugucgugucacca
ggcgcugaauauauaggcgucuggggggaacgcggggaagugaaacaucu
caguacccguaggaagagaaaacaaaaugugauuccgugaguaguggcga
gcgaaagcggaggauggcuaaaccguaugcaugugauaccggguaggggu
ugugugugcgggguugugggaccuaucuuuccggcucuaccuggcuggag
ggcagugagaaaauguugugguuagcggaaauggcuugggauggccugcc
guagacggugagagcccgguacgugaaaacccgacgucugucuugauggu
guucccgaguagcagcgggcccguggaaucugcugugaaucugccgggac
cacccgguaagccugaauacuucccagugaccgauagcggauuaguaccg
ugagggaauggugaaaaguaccccgggaggggagugaaagaguaccugaa
accgugcgcuuacaauccgucagagcccucgacgugucguggggugaugg
cgugccuuuugaagaaugagccugcgagucagggacaugucgcgagguua
acccgggugggguagccgcagcgaaagcgagucugaauagggcguaucca
cacaagagugugugguguagugguguguucuggacccgaagcggagugau
cuacccauggccagggugaagcgcggguaagaccgcguggaggcccgaac
ccacuuagguugaagacugaggggaugagcuguggguaggggugaaaggc
caaucaaacuccgugauagcugguucuccccgaaaugcauuuaggugcag
cgucgcauguuucuugccggagguagagcuacuggauggccgaugggccc
cacaggguuacugacgucagccaaacuccgaaugccgguaaguccaagag
ugcggcagugggacggcgggggauaagcuccgugcgucgagagggaaaca
gcccagaucgccggcuaaggccccuaagcgugugcuaaguggaaaaggau
gugcagucgcgaagacaaccaggagguuggcuuagaagcagccacccuug
aaagagugcguaauagcucacuggucaagugauugugcgccgauaaugua
gcggggcucaagcacaccgccgaagccgcggcagccaacguguuggcugg
guaggggagcguccugcauccggugaagccgccgagugaucgaguggugg
agggugugggagugagaaugcaggcaugaguagcgauuaggcaagugaga
accuugcccgccgaaagaccaaggguuccugggccaggccaguccgccca
gggugagucgggaccuaaggcgaggccgacaggcguagucgauggacaac
ggguugauauucccguacccguguaugugcguccaugaugaaucagcggu
acuaaccauccgcaugggaccuucguugguaguagucaagcgauggggug
acgcaggaagguagccguaccggucagugguaauaccgggguaagccugu
agggagucagauagguaaauccgucuggcauauauccugagaggugaugc
auagccgagugaggcgaauucggugauccuaugcugccgagaaaagccuc
uagcgaggacauacacggcccguaccccaaaccaacacagguggucaggu
agagaauacuaaggcguacgagugaacuaugguuaaggaacucggcaaaa
ugcccccguaacuucgggagaagggggacccacauggcguguaagccuuu
acggcccaagcgugaguggguggcacaaaccagugagaagcgacuguuua
cuaaaaacacagguccgugcgaagucgcaagacgauguauacggacugac
gccugcccggugcuggaagguuaagaggacccguuaacucccuuuggggg
ugaagcggagaauuuaagccccaguaaacggcggugguaacuauaaccau
ccuaagguagcgaaauuccuugucggguaaguuccgaccugcacgaaugg
cguaacgacuucucaacugucucaaccauagacucggcgaaauugcacua
cgaguaaagaugcucguuacgcgcggcaggacgaaaagaccccgggaccu
ucacuacaacuugguauuggugcucgauacgguuuguguaggauaggugg
gagacugugaagcucacacgccaguguggguggagucguuguugaaauac
cacucugaucguauugggccucuaaccucggaccguauauccgguucagg
gacagugccugguggguaguuuaacuggggcgguugccuccuaaaaugua
acggaggcgcccaaagguucccucaaccuggacggcaaucagguguugag
uguaagugcacaagggagcuugacugcgagacggacaugucgagcaggga
cgaaagucgggacuagugauccggcaccucugaguggaaggggugucgcu
caacggauaaaagguaccccggggauaacaggcugaucuuccccaagagu
ccauaucgacgggaugguuuggcaccucgaugucggcucgucgcauccug
gggcuggagcaggucccaaggguugggcuguucgcccauuaaagcggcac
gcgagcuggguuuagaacgucgugagacaguucggucucuauccgccgcg
cgcgucagaagcuugaggaaaccugucccuaguacgagaggaccgggacg
gacgaaccucugguauaccaguugucccaccaggggcacggcuggauagc
cacguucggacaggauaaccgcugaaagcaucuaagcgggaaaccucuuc
caagaccaggcuucucacccucuaggagggauaaggccccccgcagacca
cgggauugauagaccagaccuggaagccuaguaauaggugcagggaacug
gcacuaaccggccgaaaacuuac

<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<<......<
<<<<<.[[.(((.>>>.....<<<<<<<..<<<)))..]].>>>.....>
>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<<
<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>>.
.....>>>>>.>.........<<.....>>..<<<<<<.....<<.....
>>....>>>>>>...<<<....<<<<<<<<<<......<..<<<<<.>..
.>>>>>>>>>>......<<<<..[.....<<<<<<<>>>>>.>>>>>>><
<<<<<.......<<<<<<.<<<<.<<<.....<<<<......>>>>.((.
.....<<<.....))>>>...>>>...>>>>>>>>>>>>>>>>.....].
..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<
<<<>>>>>..>>>......>>.>>>>>>>.>>...............<<.
...>>....>>.........<<<<....>>>>..................
>>>>>>>>>>>.......<<<<..<<<<<<<<<..>>>>.>>>>>..>>>
>...<.<........>.>.<<<<<<....<<<.<<<<<<<<<<..<<<..
.<<<....>>>...>>>...<<....>>..<<<<.....>>>>....<<<
<<<<....>>>>>>>....>>>>>>>>>>>>>..<<.<..<<<<<<<...
..<<<<<<<<<..<<<....<<<<<<......>>>>>>.....>>>...<
<<.<<<<<........>>>>>>>>....>>>>>>>>>...<<<.....>>
>......>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>><
<<<<<<<<....<<<<<<<<.....<<<....<<<.<<<<....<<<<<.
.....>>>>.>....>>>>.>>>..>>.>.....>>>>>>>>.......>
>>>>>>.>>..<<<<<<<<<<......>>>>>>.>>>>....<<<.....
.>>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..
<<<<<<<<<........<<<<<.<<<..<<<<.......>>>>..>>>..
.......<<......>>...>>>>>....>>>>>>>>>.>>.........
..)))......>>>>>>.>>>...>>>>>>><<<<<<<<..>>>>>>>>.
...>>>>>><.<<<<<<<<<<......<<<<<<<<....>>>.>>>>>..
.>>>>>>>>>>..>.....>>>>>><......<<.....<<<<<<.....
..>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>
>>....((<<<<.<<...<<<...<<<....>>>...>>>...>>...<<
<<<........>>>>>))<<<<<<<<<<.......<<<<..<<<<<<<<.
..<..<<<<....>>>>.>...>>>>>>>>....>>>>.<<...<<<<<.
..<<<<..<<<..<<<<..<<<<<...((((...>>>>>.<<..<<<<..
.<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>...>>>>...>>
...<<<......>>>.....>>>>...>>>...>>>>........>>>>>
..>>....>>>>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<
<.....>>>>..>>>>..<<<<<...<<<<<<<<.<<.........<...
..<<<<<....<<..>>.....>>>>><<<<<<...<<<<....<<<...
..>>>....>>>>..>>>>>>............<<<<<<<<<..<<<...
......>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<
<<<.<<<..<<<<<<<..<<<<.<<<....<<<<<.<<<<<<...>>>>>
>..>>>>>....>>>.>>>>>>>>>..>>>>><<<<<<.......>>>>>
>........<...........<<<<<<.....>>>>>>.....>....>>
>>>..>>.>>>>>>>..>..>>.>>>>>>>>>>>>>>>.....>.((..<
<<<<......))>>>>>..<<<<<<<<.<<<.<<......<<<<<<<<<<
..<<<<<.<<<.<<<<<.<<.<<<<<<<<<<<<<...........<<<<<
...........<<<<<<<<....>>>>>>>>..................>
>>>>..>>>>>>>>>>>>>.>>...<<<.<<<<<<......>>>>>>>>>
....>>>>>.>>>..>>>>>..<<<<.....>>>><<<<<<.........
..>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<
<.[.(((>>....>>>>>>>...<<<<..<<<<....>>>>..>>>>.<<
<....>>>.>>)))]......<<<<<<<.<<..>>>>..>>>>>>>>>..
...........>>>>..>>>>>>.......<<<<.<.<.<<<<...<<..
.....>>...>>>>.>...>>>>.>.......<<<<<.<<<<<<..<<<<
<<<<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..
>...>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>>>>>>>
>>....<<<<<<<...<<<<..<<<<<<<...............>>>>>>
>.<<<<<...<<<<...<<<<<<<<<<<))))>>>>>>..>>>>>...>>
>>.>>>>>...<<<.<..<<<<<...........>>>>>...>>>>.>>>
>.......>>>>>>>..<<<<<...>>>>>....<<<<.<<<<.......
>>>>.....<<<.<<<<.<<<....<<<<<....>>>>>...>>>..>>>
>..>>>...>>>>....>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8v9l A new family of bacterial ribosome hibernation factors
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): L6 H7 A12 G14 E15 K16 K17 T20 R21 G23 R24 G25 G27 K29 K31 T32 A33 G34 R35 G36 T37 K38 T40 R43 K44 N45 F50 E51 G52 G53 Q54 M55 M59 R60 K63 K65 G66 F67 K68 N69 R70 F71 R72 V77 N79 R105 K111 L113 D115 S130 S132
• Binding residue (residue number reindexed from 1): L4 H5 A10 G12 E13 K14 K15 T18 R19 G21 R22 G23 G25 K27 K29 T30 A31 G32 R33 G34 T35 K36 T38 R41 K42 N43 F48 E49 G50 G51 Q52 M53 M57 R58 K61 K63 G64 F65 K66 N67 R68 F69 R70 V75 N77 R103 K109 L111 D113 S128 S130

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8v9l, PDBe:8v9l, PDBj:8v9l
• PDBsum: 8v9l
• PubMed: 38355796
• UniProt: A0QSG8