Structure of PDB 8uu9 Chain N Binding Site BS01

Receptor Information

>8uu9 Chain N (length=146) Species: 1642 (Listeria innocua)

MKLHELKPSEGSRKERNRVGRGTGSGNGKTSGRGHKGQKARSGGGVRLGF
EGGQLPLFRRIPKRGFTNINRKEFAIVNLDVLNRFEDGTEVTPELLIETG
IIRNEKSGIKILSDGNIEKKLTVKANKFSAAAKEAIEAAGGKTEVI

Ligand information

>8uu9 Chain A (length=2899)

uuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgaaga
aggacgggacuaacaccgauaugcuuuggggagcuguacguaagcguuga
uccagagauuuccgaaugggggaacccacuaucuuuagucggauaguauc
cuuacgugaauacauagcgugaggaaggcagacccagggaacugaaacau
cuaaguaccuggaggaagagaaagaaaaaucgauuuccugaguagcggcg
agcgaaacggaaagagcccaaaccaagaagcuugcuucuugggguuguag
gacacucuauacggaguuacaaaagaaaguuauaaaugaagcggucugga
aaggcccgccaaagacgguaacagcccgguaguugaaauggcuuucccuc
cagaguggauccugaguacgacggaacacgugaaauuccgucggaauccg
ggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaacc
aguaccgugagggaaaggugaaaagcaccccggaaggggagugaaacagu
uccugaaaccgugugccuacaaguaguuagagcccguuaaugggugauag
cgugccuuuuguagaaugaaccggcgaguuacgauuuguugcaagguuaa
gcggaaaaagcggagccguagcgaaagcgagucugaauagggcgcauaag
uaacaggucguagacccgaaaccaggugaucuacccauguccaggaugaa
gguaagguaauacuuacuggagguccgaacccacgcacguugaaaagugc
ggggaugagguguggguagcggagaaauuccaaucgaacuuggagauagc
ugguucucuccgaaauagcuuuagggcuagccucgagguaaagagucaug
gagguagagcacuguuuggacuaggggaccgaauucagauaaacuccgaa
ugccauguacuuauacucgggagucagacugcgagugauaagauccguag
ucgaaagggaaacagcccagaccaccaguuaagguccccaaauauauguu
aaguggaaaaggaugugggguugcuuagacaaccaggauguuggcuuaga
agcagccaccauugaaagagugcguaauagcucacuggucgagugacccc
gcgccgaaaauguaccggggcuaaacauauuaccgaaacuguggaugaac
cuagguucgugguaggagagcguucuaagggcggugaagucagaccggaa
ggacugguggagcgcuuagaagugagaaugccgguaugaguagcgaaaga
agggugagaaucccuuccaccgaauaucuaagguuuccugaggaaggcuc
guccgcucaggguuagucgggaccuaagccgaggccgauaggcguaggcg
auggacaacagguagagauuccuguaccagugcuaauuguuuaaccgaug
gggugacacagaaggauagggaaucgcacgaauggaaaugugcguccaag
cagugagugugagaaguaggcaaauccgcuucuugcgaagcaugagcugu
gauggggaagacggaaguuccugauuucacgcugucaagaaaagccucua
ggaagaguaguacugcccguaccgcaaaccgacacagguagaugaggaga
gaauccuaaggugagcgagagaacucucguuaaggaacucggcaaaauga
ccccguaacuucgggagaaggggugcucuauuagggugcaagcccgagag
agccgcagugaauaggcccaggcgacuguuuagcaaaaacacaggucucu
gcaaaaccguaaggugacguauaggggcugacgccugcccggugcuggaa
gguuaagaggagugcuuagcuucggcgaagguacgaauugaagccccagu
aaacggcggccguaacuauaacgguccuaagguagcgaaauuccuugucg
gguaaguuccgacccgcacgaaaggcgcaacgaucugggcacugucucaa
cgagagacucggugaaauuauaguaccugugaagaugcagguuacccgcg
acaggacggaaagaccccguggagcuuuacugcaaccugauauggaaugu
uuguaccgcuuguacaggauagguaggagccgaagagacgugugcgcuag
cauacgaggaggcaauggugggauacuacccuggcuguaugaccauucua
acccgccacgcuaagcgcguggggagacagugucaggugggcaguuugac
uggggcggucgccuccuaaagaguaacggaggcgcccaaagguucccuca
gaauggauggaaaucauucgcagaguguaaaggcacaagggagcuugacu
gcgagacugacaagucgagcagggacgaaagucgggcuuagugauccggu
gguuccgcauggaagggccaucgcucaacggauaaaagcuaccccgggga
uaacaggcuuaucucccccaagaguccacaucgacggggagguuuggcac
cucgaugucggcucgucgcauccuggggcuguagucggucccaaggguug
ggcuguucgcccauuaaagcggcacgcgagcuggguucagaacgucguga
gacaguucggucccuauccgucgcgggcgcaggaaauuugagaggagcug
uccuuaguacgagaggaccgggauggacacaccgcugguguaccaguugu
uccgccaggagcaucgcuggguagcuauguguggcagggauaaacgcuga
aagcaucuaagcgugaagccccccucaagaugagauuucccauuucuucg
gaaaguaagaucccugaaagaugaucagguagauagguuuggaguggaag
uguagcgauacauggagcggacaaauacuaaucgaucgaggacuuaacc

<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>>>.>>....<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<......<<....
.>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<<
<<<<<<<<....<<<<........<<<<<<<<<.<<<.....<<<<....
..>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>.>>>
>>>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<
<<<<..<<<<>>>>>..)))......]].>>>>>>>.]]...........
....<<....>>....]].........<<<<....>>>>...........
..}....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>
>...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...
<<.......>>...>>>...<<....>>..<<<<.....>>>>......>
>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<....
<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>>
>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>..>
.>>..<<<<<<....<<<<<....>>>>><<<<<<<<.....<<<<<<<<
<.....<<<...<<<<<<<<...<<...>>...>>>>>>>>..>>.>...
..>>>>>.>>>>...>>>>>>.>>..<<<<<<<.<<......>>.>>>>>
>>....<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<.
..........<<..<<<<<<<<<<.......<<<<<.<<...<<<<....
...>>>>...>>.........<<......>>...>>>>>...>>>>>>>>
>>.>>...........))).....>>>>>>.>>>...>>>>>>><<<<<<
<..>>>>>>>....>>>>>><.<<<<<<<.<<......<<<<<.<<....
>>.>>>>>...>>.>>>>>>>..>.....>>>>>><......<<....<<
<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<...<<...
..>>.>>>>>>....((<<<<.<<...<<<...<<<....>>>...>>>.
..>>...<<<<<........>>>>>))<<<<<<<<.<<......<<...<
<<<<...<<<<..<<<..<<<<<.<<<<<...(((....>>>>>.<<..<
<<<<..<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>..>>>>>
...>>............>>>>>...>>>...>>>>........>>>>>..
>>..>>.>>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<<..
...>>>>..>>>>..<<<<....<<<<<<<<.<<.........<.....<
<<<<....<<..>>.....>>>>><<<<<....<<<<....>>>>...>>
>>>............<<<<<<<<<..<<<.........>>>..<<<<<<<
.....<<<....>>>.......>>>>>>>..<<<<.<<<..<<<<<<<..
<<<<..<<...<<<<<<.<<<..>>>..>>>>>>...>>..>>>>>>>>>
..>>>>><<<<<<.......>>>>>>........<...........<<<<
<<.....>>>>>>.....>....>>>>...>>.>>>>>>>..>..>>.>>
>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<
<<.(((.[[......<<<<.[[<<<<..<<<<<.<<<<<<<<<.<<<<<.
<<<<<<.<<<.<.........<<<<<..<<<.......<<<<<<<....>
>>>>>>....>>>............>>>>>>.>>>.>>>>>>.>>>>>..
..<<.<<<<<<.....>>>>>>>>.....>>>>>>>>>..>>>>>..<<<
<.....>>>><<<<<<...........>>>>>>..<<...<<<<<<<...
<<<<<.<......>>>>>>.....<<.[.(((>>....>>>>>>>...<<
<<..<<<<....>>>>..>>>>.<<<....>>>.>>)))]......<<<<
<<<.((..>>>>..))>>>>>>>.............>>>>..]]>>>>..
.....<<<<.<.<<<<<<...<<.......>>...>>>>>>...>>>>.>
.......<<<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<
<<<.....>>>>>.....>>>>>..>...>>>.>>....<<<<<<<....
>>>.>>>>....]]..)))>>>>>>>>....<<<<<<<...<<<<..<<<
<<<<...............>>>>>>>.<<<<<...<<<<...<<<<<<<<
<<<.)))>>>>>>..>>>>>...>>>>.>>>>>...<<<.<..<<<<<..
.........>>>>>...>>>>.>>>>.......>>>>>>>..<<<<<..>
>>>>....<<<<<<<<<.......>>>>>....<<<<<<<<..<<....<
<<<<....>>>>>...>>...>>>>>.>>>...>>>>....>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8uu9 Mechanistic insights into the alternative ribosome recycling by HflXr.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): H4 L6 S9 G11 S12 R13 K14 R16 N17 R18 G20 R21 G22 G24 G28 K29 T30 S31 G32 R33 G34 H35 K36 G37 Q38 K39 R41 S42 G43 R47 F50 G52 G53 Q54 L55 R59 R60 P62 K63 R64 G65 F66 N68 I69 N70 R71 I76 R103 K110 L112 S113 S129
• Binding residue (residue number reindexed from 1): H4 L6 S9 G11 S12 R13 K14 R16 N17 R18 G20 R21 G22 G24 G28 K29 T30 S31 G32 R33 G34 H35 K36 G37 Q38 K39 R41 S42 G43 R47 F50 G52 G53 Q54 L55 R59 R60 P62 K63 R64 G65 F66 N68 I69 N70 R71 I76 R103 K110 L112 S113 S129

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8uu9, PDBe:8uu9, PDBj:8uu9
• PDBsum: 8uu9
• PubMed: 38407413
• UniProt: A0A0U5A6S7