Structure of PDB 8uu8 Chain N Binding Site BS01

Receptor Information

>8uu8 Chain N (length=146) Species: 1642 (Listeria innocua)

MKLHELKPSEGSRKERNRVGRGTGSGNGKTSGRGHKGQKARSGGGVRLGF
EGGQLPLFRRIPKRGFTNINRKEFAIVNLDVLNRFEDGTEVTPELLIETG
IIRNEKSGIKILSDGNIEKKLTVKANKFSAAAKEAIEAAGGKTEVI

Ligand information

>8uu8 Chain A (length=2901)

guuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgaag
aaggacgggacuaacaccgauaugcuuuggggagcuguacguaagcguug
auccagagauuuccgaaugggggaacccacuaucuuuagucggauaguau
ccuuacgugaauacauagcgugaggaaggcagacccagggaacugaaaca
ucuaaguaccuggaggaagagaaagaaaaaucgauuuccugaguagcggc
gagcgaaacggaaagagcccaaaccaagaagcuugcuucuugggguugua
ggacacucuauacggaguuacaaaagaaaguuauaaaugaagcggucugg
aaaggcccgccaaagacgguaacagcccgguaguugaaauggcuuucccu
ccagaguggauccugaguacgacggaacacgugaaauuccgucggaaucc
gggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaac
caguaccgugagggaaaggugaaaagcaccccggaaggggagugaaacag
uuccugaaaccgugugccuacaaguaguuagagcccguuaaugggugaua
gcgugccuuuuguagaaugaaccggcgaguuacgauuuguugcaagguua
agcggaaaaagcggagccguagcgaaagcgagucugaauagggcgcauaa
guaacaggucguagacccgaaaccaggugaucuacccauguccaggauga
agguaagguaauacuuacuggagguccgaacccacgcacguugaaaagug
cggggaugagguguggguagcggagaaauuccaaucgaacuuggagauag
cugguucucuccgaaauagcuuuagggcuagccucgagguaaagagucau
ggagguagagcacuguuuggacuaggguaccgaauucagauaaacuccga
augccauguacuuauacucgggagucagacugcgagugauaagauccgua
gucgaaagggaaacagcccagaccaccaguuaagguccccaaauauaugu
uaaguggaaaaggaugugggguugcuuagacaaccaggauguuggcuuag
aagcagccaccauugaaagagugcguaauagcucacuggucgagugaccc
cgcgccgaaaauguaccggggcuaaacauauuaccgaaacuguggaugaa
ccuagguucgugguaggagagcguucuaagggcggugaagucagaccgga
aggacugguggagcgcuuagaagugagaaugccgguaugaguagcgaaag
aagggugagaaucccuuccaccgaauaucuaagguuuccugaggaaggcu
cguccgcucaggguuagucgggaccuaagccgaggccgauaggcguaggc
gauggacaacagguagagauuccuguaccagugcuaauuguuuaaccgau
ggggugacacagaaggauagggaaucgcacgaauggaaaugugcguccaa
gcagugagugugagaaguaggcaaauccgcuucuugcgaagcaugagcug
ugauggggaaguacggaaguuccugauuucacgcugucaagaaaagccuc
uaggaagaguaguacugcccguaccgcaaaccgacacagguagaugagga
gagaauccuaaggugagcgagagaacucucguuaaggaacucggcaaaau
gaccccguaacuucgggagaaggggugcucuauuagggugcaagcccgag
agagccgcagugaauaggcccaggcgacuguuuagcaaaaacacaggucu
cugcaaaaccguaaggugacguauaggggcugacgccugcccggugcugg
aagguuaagaggagugcuuagcuucggcgaagguacgaauugaagcccca
guaaacggcggccguaacuauaacgguccuaagguagcgaaauuccuugu
cggguaaguuccgacccgcacgaaaggcgcaacgaucugggcacugucuc
aacgagagacucggugaaauuauaguaccugugaagaugcagguuacccg
cgacaggacggaaagaccccguggagcuuuacugcaaccugauauggaau
guuuguaccgcuuguacaggauagguaggagccgaagagacgugugcgcu
agcauacgaggaggcaauggugggauacuacccuggcuguaugaccauuc
uaacccgccacgcuaagcgcguggggagacagugucaggugggcaguuug
acuggggcggucgccuccuaaagaguaacggaggcgcccaaagguucccu
cagaauggauggaaaucauucgcagaguguaaaggcacaagggagcuuga
cugcgagacugacaagucgagcagggacgaaagucgggcuuagugauccg
gugguuccgcauggaagggccaucgcucaacggauaaaagcuaccccggg
gauaacaggcuuaucucccccaagaguccacaucgacggggagguuuggc
accucgaugucggcucgucgcauccuggggcuguagucggucccaagggu
ugggcuguucgcccauuaaagcggcacgcgagcuggguucagaacgucgu
gagacaguucggucccuauccgucgcgggcgcaggaaauuugagaggagc
uguccuuaguacgagaggaccgggauggacacaccgcugguguaccaguu
guuccgccaggagcaucgcuggguagcuauguguggcagggauaaacgcu
gaaagcaucuaagcgugaagccccccucaagaugagauuucccauuucuu
cggaaaguaagaucccugaaagaugaucagguagauagguuuggagugga
aguguagcgauacauggagcggacaaauacuaaucgaucgaggacuuaac
c

<<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<....
..<<<<<<.[...((.>>>.....<<<<<<<..<<<))...].>>>....
.>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>....<<<<<<.....<<....
>>......>>>>>.>.........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<
<<<<<<<<<....<<<<........<<<<<<<<<.<<<.....<<<<...
...>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>.>>
>>>>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......
<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]]..........
.....<<....>>....]].........<<<<....>>>>..........
...}....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>
>>...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<<<..
.<<.......>>...>>>...<<....>>..<<<<.....>>>>......
>>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<...
.<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>
>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>..
>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<.....<<<<<<<
<<.....<<<...<<<<<<<<...<<...>>...>>>>>>>>..>>.>..
...>>>>>.>>>>...>>>>>>.>>..<<<<<<<.<<......>>.>>>>
>>>....<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<
...........<<..<<<<<<<<<<<......<<<<<.<<<..<<<<...
....>>>>..>>>.........<<......>>...>>>>>..>>>>>>>>
>>>.>>...........))).....>>>>>>.>>>...>>>>>>><<<<<
<<..>>>>>>>....>>>>>><.<<<<<<<.<<......<<<<<.<<...
.>>.>>>>>...>>.>>>>>>>..>.....>>>>>><......<<....<
<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<...<<..
...>>.>>>>>>....((<<<<.<<...<<<...<<<....>>>...>>>
...>>...<<<<<........>>>>>))<<<<<<<<.<<......<<...
<<<<<...<<<<..<<<..<<<<<.<<<<<...(((....>>>>>.<<..
<<<<<..<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>..>>>>
>...>>.............>>>>>...>>>...>>>>........>>>>>
..>>..>>.>>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<<
.....>>>>..>>>>..<<<<....<<<<<<<<.<<.........<....
.<<<<<.....<..>......>>>>><<<<<....<<<<....>>>>...
>>>>>............<<<<<<<<<..<<<.........>>>..<<<<<
<<.....<<<....>>>.......>>>>>>>..<<<<.<<<..<<<<<<<
..<<<<..<<...<<<<<<.<<....>>..>>>>>>...>>..>>>>>>>
>>..>>>>><<<<<<.......>>>>>>........<...........<<
<<<.......>>>>>.....>....>>>>...>>.>>>>>>>..>..>>.
>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<
<<<<.(((.[[......<<<<.[[<<<<..<<<<<.<<<<<<<<<.<<<<
<.<<<<<<.<<<...........<<<<<..<<<.......<<<<<<<...
.>>>>>>>....>>>............>>>>>..>>>.>>>>>>.>>>>>
....<<.<<<<<<.....>>>>>>>>.....>>>>>>>>>..>>>>>..<
<<<.....>>>><<<<<<...........>>>>>>..<<...<<<<<<<.
..<<<<<.<......>>>>>>.....<<.[.(((>>....>>>>>>>...
<<<<..<<<<....>>>>..>>>>.<<<....>>>.>>)))]......<<
<<<<<.((..>>>>..))>>>>>>>.............>>>>..]]>>>>
.......<<<<.<.<<<<<<...<<.......>>...>>>>>>...>>>>
.>.......<<<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.
<<<<<.....>>>>>.....>>>>>..>...>>>.>>....<<<<<<<..
..>>>.>>>>....]]..)))>>>>>>>>....<<<<<<<...<<<<..<
<<<<<<...............>>>>>>>.<<<<<...<<<<...<<<<<<
<<<<<.)))>>>>>>..>>>>>...>>>>.>>>>>...<<<.<..<<<<<
...........>>>>>...>>>>.>>>>.......>>>>>>>..<<<<..
..>>>>....<<<<<<<<<.......>>>>>....<<<<<<<<..<<...
.<<<<<....>>>>>...>>...>>>>>.>>>...>>>>....>>>>>>>
.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8uu8 Mechanistic insights into the alternative ribosome recycling by HflXr.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): H4 L6 K7 S9 G11 S12 R13 K14 R18 G20 R21 G22 G24 G28 K29 T30 S31 G32 R33 H35 K36 Q38 K39 R41 S42 G43 R47 F50 E51 G52 G53 Q54 L55 R59 R60 P62 K63 R64 G65 F66 N68 I69 N70 R71 I76 R103 K110 L112 S113 D114 S129 A130
• Binding residue (residue number reindexed from 1): H4 L6 K7 S9 G11 S12 R13 K14 R18 G20 R21 G22 G24 G28 K29 T30 S31 G32 R33 H35 K36 Q38 K39 R41 S42 G43 R47 F50 E51 G52 G53 Q54 L55 R59 R60 P62 K63 R64 G65 F66 N68 I69 N70 R71 I76 R103 K110 L112 S113 D114 S129 A130

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8uu8, PDBe:8uu8, PDBj:8uu8
• PDBsum: 8uu8
• PubMed: 38407413
• UniProt: A0A0U5A6S7