Structure of PDB 8fn2 Chain N Binding Site BS01

Receptor Information

>8fn2 Chain N (length=145) Species: 224326 (Borreliella burgdorferi B31)

MFNLLKPKGASKRRKIVGRGPGSGLGKTSGRGQKGQKARNTSPRLGFEGG
QTPLYRRLPRKGFSNSDYKLEYAIVNLGDIDKKFKDGQVVNYDTLLENKL
IKKKNKKIKILSNGKLTKKVSFEVSKISKSAESLVIKIGCTIQLV

Ligand information

>8fn2 Chain A (length=2762)

uggucaaaguaauaagagucuauggugaaugccuaggagcuuuaaggcga
agaaggucgugguaagcugcgaaaagcuugggggagaagcaaacauuuau
ugaucccaagauuaccgaauggaguaauccagcuagcaagaugcuagcua
ucuauuauuuaaauaauagaggcgauaccaggggaagugaaccaucuaag
uacccugaggaaaagaaaucaaagagauucccuuaguaguggcgagcgaa
aagggaguagcccaaacuuuaaaugugucaagcugcagagcguugcauuu
augggguuguaggacguuuaggcuuagucuguaauaagcaaaaaaguuac
aaaauauuuauauagaagaauaaucuggaaaguuuaaccaaagaagguga
uaguccuguaauuuaaauguaaauaucuuuuuaaaauguuccugaguagg
acgaggcacgagaaaccuuguuugaagcuggggagaccacucuccaaggc
uaaauacuagaaagcuaccgauagagaagaguaccgugagggaaagguga
aaagaaccccgggaggggagugaaauagaacugaaaccguagacuuacaa
gcagucaaagccguaauuuauugcggugauggcgugccuuuugcauaaug
aaccugcgaguuaucaugucuagcaagauuaaagcauagaagugcuggag
ucgaagcgaaagcgagucuuaaaagggcgauuuaguuagaugugguagac
ccgaagccgagugaucuauuuauggccaggcugaagcuuggguaaaacca
aguggagggccgaacucuagucuguuuaaaaaggcagggaugagcuguga
auaggagugaaaggcuaaacaaacucggagauagcugguucuccccgaaa
uggauuuaaguucagccuuauuuuaguuuaauagagguagagcacuaauu
gagcuagggccugucaaaggguaccaaacucaguuaaacuccgaaugcua
uuaaaugaugaauaggagugagacuaugggcgauaagguucauagucgag
agggaaacaacccagaccaacagcuaaggucucaaaaauguguuaagugg
aaaaggagguuuagguacguaaacagccaggagguuggcuuagaagcagc
cauaccuuuaaagagugcguaauagcucacuggucgaguacuuaagcgcc
gauaauguaacggggcuaaacacauuaccgaagcuuuggaucuuaacgaa
aguuaagaugguaggggagcguucuguaagccagagaaguuaagcuggaa
aguuugauggagguaucagaagugagaaugcagguaugaguaacgaaaaa
augggugagauucccauucgccgaaaaccuaagguuuccuggguaaaggu
cgucuucccaggguuagucggccccuaaggcaaagcugaaaaguguaguc
gaugggaaacggguuaauauucccguaccucuuauaguuucgauggagug
acgcaugagguuaacuacugcuaggcgaugguuguccuaguuuaagcauu
aaggcgaugaucuuaauaggaaaauccguuaagagagcuaagaugugaug
augagugcuauuuagguagcaugaaauguagguagucaaggugccaagaa
auagcuucuaagguuaggcuauaagggaccguaccgcaaaccgacacagg
uaggugggaugaaaauucuaaggcgcgcgagagaauccacguuaaggaac
ucugcaaaauacguacguaacuucgggauaaguacgaccuaagcaauuag
guagcauaaaaaugguccaaacgacuguuuaccaaaaacacaggucucug
caaaucuguaaagagaaguauagggacugacaccugcccggugcuaguaa
acggcuaagguagccggguaaguuccgacgaaugguguaacgauuuggac
gcugucucaacguggagcucggugaaauugaaguaucggugaagaugccg
auuacuugugguuagacggaaagaccccgugaaccuuuacuauagcuugg
uauugagauuugauuaauuaauuagguuucuaacuuauagaaauaugagg
agagugccagguggguaguuugacuggggcggucgccuccuaaagaguaa
cggaggugcgcaaagguuaccuuagagugguuggaaaucacucuguaagu
guaaaggcauaagguagcuuaacuguaagacugacaagucgaacagauac
gaaaguaggucuuagugaucuggcgguggcaaguggaagcgccgucacuu
aacgaauaaaagguacuccggggauaacaggcuuauccuucccaagaguu
cacaucgacggaaggguuuggcaccucgaugucggcucaucgcauccuag
ggcuggagcagguccuaaggguauggcuguucgccauuuaaagcgguacg
cgagcuggguucagaacgucgugagacaguuuggucccuaucugccacaa
gcguuggauauuugagaggagcuaucuuuaguacgagaggaccgagaugg
acgaaccucuagugugccaguuauccugccaaggguaagugcuggguagc
uacguucggaaaggauaaccgcugaaagcaucuaagugggaagccuuccu
caagaugagauauccuuuaaggguccuggaagaauaccagguugauaggu
uagaaguguaaguauagcaauauauuaagcugacuaauacuaauuacccg
uaucuuuggcca

.<<<<<<<<......<<<<<<<<<<<.....<<..<<<<<<<<.<<<<..
....<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>..
...>>>>>>>...>>>....<<<....>>><<<<<<<.....>>>>>>>.
<<<<<<<.<..>.>>>>>>>.>>....<<<<<<.....<<....>>....
..>>>>>.>.........<<...>>..<<<<<<.....<<.....>>...
.>>>>>>..<<<....<<.<<<<<<<.......<<.<<<.....>>>>>>
>.>>......[<<<<.[....<<<<<.>>>.>>.>>>>>.<<<<......
..<.<<<<.<<<<.<<....<<<.<......>.>>>((......<<<...
..))>>>....>>...>>>>>>.>>>.>>>>...]..>>>>.].....<<
<<<<<<.........>>>>>>>>......<<<<<..((((>>>>>..>>>
......>>..>>>>>>.>>..............<<....>>....>>...
......<<<<....>>>>.................>>>>>>>>>>>....
...<<<<..<<<<<<<....>>>>>>>..>>>>...<.<........>.>
.<<<<<<....<<<<<<<<<<<<<..<<<...<<<<<....>>>>>...>
>>...<<....>>..<<<<.....>>>>......>>>>>>>>>>>>>..<
<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<......>>>
>>>.....>>>...<<<<.<<<<........>>>>>>>>....>>>>>>>
>>...<<<.....>>>......>>>>>>...>..>.>>..<<<<<<....
<<<<<....>>>>><<<<<<<<<..<<<<<<<<<.....<<<...<<<<<
<<<...<<<<<<......>>>>.>>...>>>>>>>>..>>.>.....>>>
>>>>>>...>>>>>>>.>>..<<<<<<<<<<......>>>>>>>>>>...
.<<<......>>>...<<<<.<<<...<<<((....<<<<<<<.......
....<<..<<<<<<<<<........<<<<<.<<...<<<.........>>
>....>>.........<<......>>...>>>>>....>>>>>>>>>.>>
............)).....>>>>>>>>>>...>>>>>>><<<<<<<<...
.>>>>>>>>....>>>>>>..<<<<<...<<<......<<<<<<<<....
>>>>>>>>...>>>..>>>>>........>>>>>><......<<.....<
<<<<<.......>>>>>><<<<....<<<<<.<<<..<<<<<<...<<..
...>>.>>>>>>....((<<<.<<<...<<<...<<<....>>>...>>>
...>>>..<<<<<........>>>>>)).<<<<<<<<<.<<...<<<<<.
..<<<<..<<<...<<<<.<<<<<<...[[[[...>>>>>><<..<<<<<
..<<<........<<<............>>>.....>>>..>>>>>...>
>...<<<<<<<....>>>>>>>.....>>>>...>>>...>>>>......
...>>>>>...>>....>>>>>>>>>..>>>.>>>.....<<......>>
>>>>>.<<<.......>>>..>>>>..<<<<....<<<<<<<<.<<....
.....<.....<<<<<...............>>>>><<<<<<....>>>>
>>............<<<<<<<<<..<<<.........>>>..<<<<<<<.
....<<<....>>>.......>>>>>>>.<<<<<..<<..<<<<..>>..
>>>>........<.<<<.......>>>.>....>>>>>..>>.>>>>>>>
..>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>
>>..<<<<<<<<.<<<.<<......<<<<.<<<<<<..<<<<<.<<<<<<
<<<.<<<<<<<<<<<<..>>>>>>>>>>>>...<<<<<<....>>>>>>.
...>>>>>>>>>..>>>>>..<<<<.....>>>><<<<<<..........
.>>>>>>..<<...<<<<<<<.<<<<<<<<.......>>>>>>>>...<<
.[.(((>>....>>>>>>>...<<<<..<<<<....>>>>..>>>>.<<<
....>>>.>>)))]......<<<<<<<.<<..))))..>>>>>>>>>...
..........>>>>..>>>>>>.......<<<<.<.<<<<<<...<<...
....>>...>>>>>>...>>>>.>.......<<<<<.<<<<<<..<<<<<
<<<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>
...>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>>>>>>>>
>....<<<<<<<...<<<<..<<<<<<<...............>>>>>>>
.<<<<<...<<<<...<<<<<<<<<<<]]]]>>>>>>...>>>>>...>>
>>.>>>>>..<<<<....<<<<<...........>>>>>....>>>>>>>
>.......>>>>>>>.....<<<<<<<<<.......>>>>>....<<<<<
<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>.
...>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fn2 The structure of a hibernating ribosome in a Lyme disease pathogen.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): N3 K6 G9 S11 K12 R13 R14 K15 V17 G18 R19 G20 P21 G22 S23 G26 K27 T28 S29 G30 R31 G32 Q33 K34 G35 Q36 K37 R39 N40 R44 F47 G49 G50 Q51 R56 R57 P59 R60 K61 N65 D67 Y68 I74 K102 K103 K104 K109 L111 S112 N113 K129 S130
• Binding residue (residue number reindexed from 1): N3 K6 G9 S11 K12 R13 R14 K15 V17 G18 R19 G20 P21 G22 S23 G26 K27 T28 S29 G30 R31 G32 Q33 K34 G35 Q36 K37 R39 N40 R44 F47 G49 G50 Q51 R56 R57 P59 R60 K61 N65 D67 Y68 I74 K102 K103 K104 K109 L111 S112 N113 K129 S130

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fn2, PDBe:8fn2, PDBj:8fn2
• PDBsum: 8fn2
• PubMed: 37907464
• UniProt: O51450|RL15_BORBU Large ribosomal subunit protein uL15 (Gene Name=rplO)