Structure of PDB 8cas Chain N Binding Site BS01

Receptor Information

>8cas Chain N (length=53) Species: 580240 (Saccharomyces cerevisiae W303)

ENVWFSHPRRYGKGSRQCRVCSSHTGLIRKYGLNICRQCFREKANDIGFN
KFR

Ligand information

>8cas Chain 2 (length=1763)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuacaugguauaacugugguaau
ucuagagcuaauacaugcuuaaaaucucgacccuuuggaagagauguauu
uauuagauaaaaaaucaaugucuucggacucuuugaugauucauaauaac
uuuucgaaucgcauggccuugugcuggcgaugguucauucaaauuucugc
ccuaucaacuuucgaugguaggauaguggccuaccaugguuucaacgggu
aacggggaauaaggguucgauuccggagagggagccugagaaacggcuac
cacauccaaggaaggcagcaggcgcgcaaauuacccaauccuaauucagg
gagguagugacaauaaauaacgauacagggcccauucgggucuuguaauu
ggaaugaguacaauguaaauaccuuaacgaggaacaauuggagggcaagu
cuggugccagcagccgcgguaauuccagcuccaauagcguauauuaaagu
uguugcaguuaaaaagcucguaguugaacuuugggcccgguuggccgguc
ggauuuccaacggggccuuuccuucuggcuaaccuugagcuuggcgaacc
aggacuuuuacuuugaaaaaauuagaguguucaaagcaggcguauugcuc
gaauauauuagcauggaauaauagaauaggacguuugguucuauuuuguu
gguuucuaggaccaucguaaugauuaauagggacggucgggggcaucagu
auucaauugucagaggugaaauucuuggauuuauugaagacuaacuacug
cgaaagcauuugccaaggacguuuucauuaaucaagaacgaaaguuaggg
gaucgaagaugaucagauaccgucguagucuuaaccauaaacuaugccga
cuagggaucgggugguguuuuuuuaaugacccacucggcaccuuacgaga
aaucaaagucuuuggguucuggggggaguauggucgcaaggcugaaacuu
aaaggaauugacggaagggcaccaccaggaguggagccugcggcuuaauu
ugacucaacacggggaaacucaccagguccagacacaauaaggauugaca
gauugagagcucuuucuugauuuugugggugguggugcauggccguucuu
aguugguggagugauuugucugcuuaauugcgauaacgaacgagaccuua
accuacuaaauaguggugcuagcauuugcugguuauccacuucuuagagg
gacuaucgguuucaagccgauggaaguuugaggcaauaacaggucuguga
ugcccuuagacguucugggccgcacgcgcgcuacacugacggagccagcg
agucuaaccuuggccgagaggucuugguaaucuugugaaacuccgucgug
cuggggauagagcauuguaauuauugcucuucaacgaggaauuccuagua
agcgcaagucaucagcuugcguugauuacgucccugcccuuuguacacac
cgcccgucgcuaguaccgauugaauggcuuagugaggccucaggaucugc
uuagagaagggggcaacuccaucucagagcggagaauuuggacaaacuug
gucauuuagaggaacuaaaagucguaacaagguuuccguaggugaaccug
cggaaggaucauu

...<<<<<.{.[[[[>>>>><<<<.<<<<<<...<.<...<......<<<
.<<<..<<....<<....<<..........>>...>>.>>......<<..
......<<<..<<..<<....<<<..........<.....<<.<<<....
.>>>.>>......>.........<<<<<..<<....>>..>>>>><.<.<
<<<<<......(((((((.<<<....>>>..............>>>>>>.
>...>...<<<<..<<<.....>>>.>>>>....>>>...>>>>..>>>.
<<<....<<<....<<<<<<<<.......>>>>>>>>>>>......>>>.
..<<<.<<<<....>>>>....>>>.>>.<<.<<<..........>>>.>
>.<.<<<..>>>.>...>>>>>>.........<<<....<<<.....>>>
..>>>..>....>.>.....<<<<<<<.<.<<<....>>>.>.>>>>.>>
>......<<..<...........>..>>.........<<<<<((....<.
<<<<.....<<..))>>.......>>>>>>>>>>..>>>>>>>>>>....
.....<<<[[.....<.<<...<<<.<<....<<<<<<.<<<<.......
.......>>>>.>>>>>>....<<<<<<....................>>
>>>>...<<<<<.<<.......<<...<.......>..<<<<...>>>>.
...>>......>>.>>..>>>.........<<<..<<<<<<<<.....))
))))).>>>>>>>>.>>>.....>>....<<<<<<.<<...<<<<..<<.
.<<<<<<.<...<<<......>>>......>.>>>>>>..>>.......<
<....>>...>>>>...>>>>>.>>>...>>>...>>.>....<<<<<<<
...<...<<<<.<.....>.>>>>...>>>>>>>>..........<<<.<
<.<<<..<<<<<<<<.<<<........>>>>>>>>>>>..>>>...<<..
]]>>...>>.....>>>.>>>.<<<......<<<<....>>>>....>>>
..]]]].}<<<<<.<<<<<<<..<<.<<<<<<..<<<.<<<<<<......
<<........>>..........<<<<<.<....<<<<<........<<.<
<<........>>>.>>......>>>>>...<<.<<<..<<.<<<<<<...
.<<<.<<<<<....>>>...<<<......>>>...>>.>>>....<<<<<
.<<..<<<<..<<<<<.<<<<<<....>>>>>>...>>>>>..>>>>.>>
....<<<<<<.....>>>>>>.......>>>>>....>>>.>>>.....>
>>>>>>.....>.>>>>>...>>.>>>>.>>>.....<<<<<<<......
<<.....<<..<<<<....>>>>..>>....>>.......>>>>>>>...
...<....<<<<<<..........>>>>>>....>.....>>>>>>....
<<<<<<<<.......>>>>>>>>......>>...>>>>>>>>>>.>>...
.<..<<.<..<<<<.<<....<<<<<<<<.<<<..<.<<..<...<<<<<
<<.<<<<.<<<<....>>>>.>>>>.>>>>>>>...>..>>.>..>>>.>
>>>>>>>...>>.>>>>...>.>>...>.....<<<<<<<<<<..>>>>>
>>>>>........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8cas Molecular basis for recognition and deubiquitination of 40S ribosomes by Otu2.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): V6 F8 S9 H10 K16 G17 G29 L30 R32 Q41 R56
• Binding residue (residue number reindexed from 1): V3 F5 S6 H7 K13 G14 G26 L27 R29 Q38 R53

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8cas, PDBe:8cas, PDBj:8cas
• PDBsum: 8cas
• PubMed: 37169754
• UniProt: P41057|RS29A_YEAST Small ribosomal subunit protein uS14A (Gene Name=RPS29A)