Structure of PDB 6zxh Chain N Binding Site BS01

Receptor Information

>6zxh Chain N (length=149) Species: 9606 (Homo sapiens)

GRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIG
VILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKH
LERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALV

Ligand information

>6zxh Chain 2 (length=1651)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggcuacagugaaacugcgaauggcucauuaa
aucaguuaugguuccuuuggucgcucguacuuggauaacugugguaauuc
uagagcuaauacaugccgacgggcgcugacccccuucgcgggggggaugc
gugcauuuaucaguggugacucuagauaaccucgggccgaucgcacgcgc
ggcgacgacccauucgaacgucugcccuaucaacuuucgaugguagucgc
cgugccuaccauggugaccacgggugacggggaaucaggguucgauuccg
gagagggagccugagaaacggcuaccacauccaaggaaggcagcaggcgc
gcaaauuacccacucccgacccggggagguagugacgaaaaauaacaaua
caggacucuuucgaggcccuguaauuggaaugaguccacuuuaaauccuu
uaacgaggauccauuggagggcaagucuggugccagcagccgcgguaauu
ccagcuccaauagcguauauuaaaguugcugcaguuaaaaagcucguagu
uggaucucucucggccgaagcguuuacuuugaaaaaauuagaguguucaa
agcaggccgccuggauaccgcagcuaggaauaauggaauaggaccgcggu
ucucggaacugaggccaugauuaagagggacggccgggggcauucguauu
gcgccgcuagaggugaaauucuuggaccggcgcaagacggaccagagcga
aagcauuugccaagaauguuuucauuaaucaagaacgaaagucggagguu
cgaagacgaucagauaccgucguaguuccgaccauaaacgaugccgaccg
gcgaugcggcggcguuauaugacccgccgggcagcuuccgggaaaccaaa
gucuuuggguuccggggggaguaugguugcaaagcugaaacuuaaaggaa
uugacggaagggcaccaccaggaguggagccugcggcuuaauuugacuca
acacgggaaaccucacccggcccggacacggacaggauugacagauugau
agcucuuucucgauuccgugggugguggugcauggccguucuuaguuggu
ggagcgauuugucugguuaauuccgauaacgaacgagacucuggcaugcu
aacuaguuacgcgacccccggucggcguaacuucuuagagggacaagugg
cguucagccacccgagauugagcaauaacaggucugugaugcccuuagau
guccggggcugcacgcgcgcuacacugacuggcucagcgugugccuaccc
uacgccggcaggcgcggguaacccguugaaccccauucgugauggggauc
ggggauugcaauuauuccccaugaacgaggaauucccaguaagugcgggu
cauaagcuugcguugauuaagucccugcccuuuguacacaccgcccgucg
cuacuaccgauuggaugguuuagugaggcccucggaucggccccgccggg
gugcccuggcggagcgcugagaagacggucgaacuugacuaucuagagga
aguaaaagucguaacaagguuuccguagguaaaccugcggaaggaucauu
a

...<<<<<.[.((((>>>>><<<.<<<<<<...<.<<..<......<<<.
<<<..<<....<<....<<.......>>...>>.>>......<<..<...
..<<<.<....<<....<<<<..........<<.....<<.<<<.....>
>>.>>......>>.........<<<<...<<<<<.....>>.>>>...>>
>><<..<<<<<...<......>..>>>>>......>>...<<<<.<<..>
>>>>>..>.>>>...>>..>.>>>.<<<....<<<....<<<<<<<....
.....>>>>>>>>>>......>>>...<<<.<<<<....>>>>....>>>
>>>.<<.<<<..........>>>.>>.<.<<....>>.>...>>>>>>..
.......<<<....<<<<...>>>>..>>>..>...>>.>.....<<<<<
<<<<.<<<....>>>..>>>>>>.>>>......<<..<...........>
..>>.........<<<<<((....<.<<<<.....<<..))>>.......
>>>>>>>>>>..>>>>>>.>>>.........<.<((.....<.<<...<<
<.<<...<<.<<<..>>>>>...<<<<<<.<.......<<...<......
.>.<<<<.>>>>...>>......>.>>>..>>>........<.<<.<<<<
<<...>>>>>>.>>.>....>>....<<<<<<..<...<<<<..<<..<<
<<<<<<...<<<......>>>......>>>>>>>>..>>.......<<..
..>>...>>>>..>..>>>.>>>...>>>...>>.>....<<<<<<<...
<...<<<<.<.....>.>>>>...>>>>>>>>..........<<<.<<.<
<<..<.<<<<<<.<<<<<>>>>>>>>>>>.>..>>>...<<..))>>...
>>.....>>>.>.>.<<<......<<<......>>>....>>>..)))).
]<<<<<.<<<<<<<..<<..<<<<<..<<<.<<<<<<......<<.....
...>>..........<<<<<......<<<<<<.......<<.<<<.....
...>>>.>>.....>>>>>>.<.<<.<<<..<<.<<<<<<....<<<.<<
<<<....>>>...<<<......>>>...>>.>>>....<<<...<...<<
<<..<<<<<<<<<<<<...>>>>.>>>>>>>>..>>>>..>.....<<<<
<.....>>>>>........>>>....>>>.>>>.....>>>>>>>>....
..>>>>>...>>.>>>>.>>>.....<<.<<<........<.......<<
<.<<<<....>>>>.>>>....>.........>>>.>>............
<<<<<..........>>>>>...........>>>>>.....<<<<<<<<.
......>>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<.
.<<<<.<<....<<<<<<<<.<<<..<<<<.<<...<<<<<<<<<<<.<<
<..>>>.>>>>>.>.>>>>>...>>.>>>>..>>>.>>>>>>>>...>>.
>>>>...>.>>...>.....<<<<<<<<<....>>>>>>>>>........
.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6zxh Structural basis for the final steps of human 40S ribosome maturation.
• Resolution: 2.7 Å
• Binding residue (original residue number in PDB): G2 R3 M4 H5 G8 K9 G10 L11 S12 Q13 S14 A15 P17 R20 P47 S48 G51 R55 A61 Q62 R64 K70 I71 L72 R73 D87 H90 K94 V98 H101 R104 N105 K107 D108 K109 K112 F113 R114 I116 L117 S120 R121 R124 R127 Y128
• Binding residue (residue number reindexed from 1): G1 R2 M3 H4 G7 K8 G9 L10 S11 Q12 S13 A14 P16 R19 P46 S47 G50 R54 A60 Q61 R63 K69 I70 L71 R72 D86 H89 K93 V97 H100 R103 N104 K106 D107 K108 K111 F112 R113 I115 L116 S119 R120 R123 R126 Y127

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0048027 mRNA 5'-UTR binding
• GO:0070181 small ribosomal subunit rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0033119 negative regulation of RNA splicing
• GO:0042274 ribosomal small subunit biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0005925 focal adhesion
• GO:0014069 postsynaptic density
• GO:0016020 membrane
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0032040 small-subunit processome
• GO:0070062 extracellular exosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6zxh, PDBe:6zxh, PDBj:6zxh
• PDBsum: 6zxh
• PubMed: 33208940
• UniProt: P62277|RS13_HUMAN Small ribosomal subunit protein uS15 (Gene Name=RPS13)