Structure of PDB 6wru Chain N Binding Site BS01

Receptor Information

>6wru Chain N (length=50) Species: 1280 (Staphylococcus aureus)

AVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCKNCGSYNG

Ligand information

>6wru Chain 1 (length=2665)

uaaguuauuaagggcgcacgguggaugccuuggcacuagaagccgaugaa
ggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuugau
ccagagauuuccgaauggggaaacccagcaugaguuaugucauguuaucg
auaugugaauacauagcauaucagaaggcacacccggagaacugaaacau
cuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggcg
agcgaaacgggaagagcccaaaccaacaagcuugcuuguugggguuguag
gacacucuaguuacaaaggacgacauuagacgaaucaucuggaaagauga
aucaaagaagguaauaauccuguagucgaaaauguugucucucuugagug
gauccugaguacgacggagcacgugaaauuccgucggaaucugggaggac
caucuccuaaggcuaaauacucucuagugaccgauagugaaccaguaccg
ugagggaaaggugaaaagcaccccggaaggggagugaaauagaaccugaa
accgugugcuuacaaguagucagagcccguuaaugggugauggcgugccu
uuuguagaaugaaccggcgaguuacgauuugaugcaagguuaagcaguaa
auguggagccguagcgaaagcgagucugaauagggcguuuaguauuuggu
cguagacccgaaaccaggugaucuacccuuggucagguugaaguucaggu
aacacugaauggaggaccgaaccgacuuacguugaaaagugagcggauga
acugaggguagcggagaaauuccaaucgaaccuggagauagcugguucuc
uccgaaauagcuuuagggcuagccucaagugaugauuauuggagguagag
cacuguuuggacucagacaaacuccgaaugccaauuaauuuaacuuggga
gucagaacaugggugauaagguccguguucgaaagggaaacagcccagac
caccagcuaaggucccaaaauauauguuaaguggaaaaggauguggcguu
gccgugacacugcgccgaaaauguaccggggcuaaacauauuaccgaagc
uguggauuguccuuuggacaaugguaggagagcguucuaagggcguugaa
gcaugaucguaaggacauguggagcgcuuagaagugagaaugccggugug
aguagcgaaagacgggugagaaucccguccaccgauugacuaagguuucc
agaggaaggcucguccgcucuggguuagucggguccuaagcugaggccga
caggcguaggcgauggauaacagguugauauuccuguaccaccuauaucg
auggggggacgcaguaggauaggcgaagcgugcgauuggauugcacgucu
aagcaguaaggauccgguacgcugagcugugauggggagaagacauugug
ucuucgagucguugauuucacacugccgagaaaagccucuagggugcccg
uaccgcaaaccgacacagguagucaagaugagaauucuaaggugagcgag
cgaacucucguuaaggaacucggcaaaaugaccccguaacuucgggagaa
ggggugcucuuuagggucccagaagagccgcagugaauaggcccaagcga
cuguuuaucaaaaacacaggucucugcuaaaccguaaggugauguauagg
ggcugacgccugcccagugcuggaagguuaagaggagugguuagcuucug
cgaagcuacgaaucgaagccccaguaaacggcggccguaacuauaacggu
ccuaagguagcgaaauuccuugucggguaaguuccgacccgcacgaaagg
cguaacgauuugggcacugucucaacgagagacucggugaaaucauagua
ccugugaagaugcagguuacccgcgacaggacggaaagaccccguggagc
uuuacuguagccugauauugaaaugcuuucuaacccgcaccacuuaucgu
ggugggagacagugucaggcgggcaguuugacuggggcggucgccuccua
aaagguaacggaggcgcucaaagguucccucagaaauucauagaguguaa
aggcauaagggagcuugacugcgagaccuacaagucgagcagggucgaaa
gacggacuuagugauccggugguuccgcauggaagggccaucgcucaacg
gauaaaagcuaccccggggauaacaggcuuaucucccccaagaguucaca
ucgacggggagguuuggcaccucgaugucggcucaucgcauccuggggcu
guagucggucccaaggguugggcuguucgcccauuaaagcgguacgcgag
cuggguucagaacgucgugagacaguucggucccuauccgucgugggcgu
aggaaauuugagaggagcuguccuuaguacgagaggaccgggauggacau
accucugguguaccaguugucgugccaacggcauagcuggguagcuaugu
guggacgggauaagugcugaaagcaucuaagcaugaagccccccucaaga
ugagauuucccaacuucgguuauaagaucccucaaagaugaugagguuaa
uagguucgagguggaagcauggugacauguggagcugacgaauacuaauc
gaucgaagacuuaau

<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<......
<<<<<<.[...((.>>>.....<<<<<<<..<<<))...].>>>.....>
>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<<
<<<<<<.........>>>>>>>.>>.>>....<<<<<......<......
>.......>>>>.>.........<<....>>..<<<<<......<<....
.>>.....>>>>>..(((....<<<<<<........>>>>>>......<<
<<<<<<<...........<.<<<<<<<.<<<...<<<<<......>>>>>
((......<<<.....))>>>...>>>...>>>>>>>..>.....>>>>>
..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<
<<<>>>>>..)))......]].>>>>>>>.]]...............<<.
...>>....]].........<<<<....>>>>.............}....
>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>>...<.<
........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...<<<....
.>>>...>>>...<<....>>..<<<<.....>>>>.....>>>>>.>>>
>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<..
....>>>>>>.....>>>...<<<.<<<<<........>>>>>>>>....
>>>>>>>>>...<<<.....>>>......>>>>>>...>..>.>>..<<<
<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.....<<<
...<<<<<<<..>>>>>>>..>>.>.....>>>>.>>>>>..>>>>>>>.
>>..<<<<<<<<<<......>>>>>>>>>>....<<<......>>>...<
<<<.<<<...<<<(((.....<<<<<<...........<<..<<<<.<<<
<...>>>>.>>>>.>>...........))).....>>>>>>.>>>...>>
>>>>><<<<<<.....>>>>>>....>>>>>><.<<<<<<<.<<......
<<<<<.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>>>><..
....<<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<
<<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<..
..>>>...>>>...>>...<<<<<........>>>>>))<<<<.......
..<<<<....<<<<...<<...<....<.<<<.....{...>>>.>.<..
..<<<<...<<..........>>...>>>>.....>.....<<.......
..>>........>....>>...>>>>.........>>>.>..>>>>>>>>
..>>.....<<......>>>>>>>.<<<.......>>>..>>>>..<<<<
....<<<<<<<<.<<.........<.....<<<<<....<....>.....
>>>>><<<<<<<.<<<.>>>.>>>>>>>............<<<<<<<<<.
.<<<.........>>>..<<<<<<<.....<<<....>>>.......>>>
>>>>.<<<<<.<<<..<<<<<<<..<<<<..<<...<<<<<<.<<....>
>..>>>>>>...>>..>>>>>>>>>..>>>>><<<<<<.......>>>>>
>........<...........<<<<<.......>>>>>.....>....>>
>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>.....<
<<<<........>>>>>..<<<<<<<<.(((.[[......<<<<.[[<<<
<..<<<<<.<<<<<<<<<.<<<<...>>>>....<<.<<<<<<.....>>
>>>>>>.....>>>>>>>>>..>>>>>..<<<<.....>>>><<<<<<..
.........>>>>>>..<<...<<<<<<<...<<<.>>>.....<<.[.(
((>>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....
>>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>.......
......>>>>..]]>>>>.......<<<<.<.<<<<<<...<<.......
>>...>>>>>>...>>>>.>.......<.<<<.<<<<<<..<<<<<<<<<
......>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>...>
>>..>....<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>...
.<<<<<<<...<<<<..<<<<<<<...............>>>>>>>.<<<
<<...<<<<...<<<<<<<<<<<.}..>>>>>>..>>>>>...>>>>.>>
>>>...<<<.<..<<<<<...........>>>>>...>>>>.>>>>....
...>>>>>>>.<<<....>>>....<<<<<<<<<.......>>>>>....
<<<<<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...
>>>>....>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6wru Characterization of the Core Ribosomal Binding Region for the Oxazolidone Family of Antibiotics Using Cryo-EM.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): A2 V3 P4 K5 R6 R7 T8 S9 T11 R12 K13 K15 R16 R17 T18 H19 K21 S39 H40 R41 Y49 G51
• Binding residue (residue number reindexed from 1): A1 V2 P3 K4 R5 R6 T7 S8 T10 R11 K12 K14 R15 R16 T17 H18 K20 S38 H39 R40 Y48 G50

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6wru, PDBe:6wru, PDBj:6wru
• PDBsum: 6wru
• PubMed: 32566908
• UniProt: Q2FZF1|RL32_STAA8 Large ribosomal subunit protein bL32 (Gene Name=rpmF)