Structure of PDB 7qvp Chain Mi Binding Site BS01

Receptor Information

>7qvp Chain Mi (length=101) Species: 9606 (Homo sapiens)

ALRYPMAVGLNKGHKVTKNVSKPRHSRRRGRLTKHTKFVRDMIREVCGFA
PYERRAMELLKVSKDKRALKFIKKRVGTHIRAKRKREELSNVLAAMRKAA
A

Ligand information

>7qvp Chain L6 (length=3572)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggacau
guggcguacggaagacccgcuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcccgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccggaggauucaacccggcggcggguccggccgugucggcgg
cccggcggaucuuucccgcgcgggggaccgucccccgaccggcgaccggc
cgccgccgggcgcauuuccaccgcggcggugcgccgcgaccggcuccggg
acggcugggaaggcccggcggggaagguggcucgggggcccccgaguguu
acagcccccccggcagcagcacucgccgaaucccggggccgagggagcga
gacccgucgccgcgcucuccccccucccgggggggccgggccaccccucc
cacggcgcgaccgcucggggcggacuguccccagugcgccccgggcgggu
cgcgccgucgggcccgggggaggccacgcgcgcgucccccgagggggacg
gcggagcgagcgcacggggucggcggcgacgucggcuacccacccgaccc
gucuugaaacacggaccaaggagucuaacacgugcgcgagucgggggcuc
gcacgaaagccgccguggcgcaaugaaggugaaggccggcgcgcucgccg
gccgaggugggaucccgaggccucuccaguccgccgagggcgcaccaccg
gcccgucucgcccgccgcgccggggagguggagcacgagcgcacguguua
ggacccgaaagauggugaacuaugccugggcagggcgaagccagaggaaa
cucugguggagguccguagcgguccugacgugcaaaucggucguccgacc
uggguauaggggcgaaagacuaaucgaaccaucuaguagcugguucccuc
cgaaguuucccucaggauagcuggcgcucucgcagacccgaccgccacgc
aguuuuauccgguaaagcgaaugauuagaggucuuggggcucucaaccua
uucucaaacuuuaaauggguaagaagcccggcucgcuggcguggagccgg
gcguggaaugcgagugccuagugggccacuuuugguaagcagaacuggcg
cugcgggaugaaccgaacgccggguuaaggcgcccgaugccgacgcucau
cagaccccagaaaagguguugguugauauagacagcaggacccgcuaagc
accugccgaaucaacuagcccugaaaauggauggcgcuggagcgucgggc
ccauacccggccgucgccggcagucgagaguggacgggagcgcggcgcgc
gccccgcggacgcuacgccgcgacgaguaggagggccgcugcggugagcc
uugaagccuagggcgcgggcccggguggagccgccgcaggugcagaucuu
ggugguaguagcaaauauucaaacgagaacuuugaaggccgaaguggaga
aggguuccaugugaacagcaguugaacaugggucagucgguccugagaga
ugggcgagcgccguuccgaagggacgggcgaccguugcccucggccgauc
gaaagggagucggguucagauccccgaauccggaguggcggagaugggcg
ccgcgaggcguccagugcgguaacgcgaccgaucccggagaagccggcgg
gagccccggggagaguucucuuuucuuugugaagggcagggcgcccugga
auggguucgccccgagagaggggcccgugccuuggaaagcgucgcgguuc
cggcggcguccggugagcucucgcuggcccuugaaaauccgggggagagg
guguaaaucucgcgccgggccguacccauauccgcagcaggucuccaagg
ugaacagccucuggcauguuggaacaauguagguaagggaagucggcaag
ccggauccguaacuucgggauaaggauuggcucuaagggcuggguccggc
cggcgccuagcagccgacuuagaacuggugcggaccaggggaauccgacu
guuuaauuaaaacaaagcaucgcgaaggcccgcggcggguguugacgcga
ugugauuucugcccagugcucugaaugucaaagugaagaaauucaaugaa
gcgcggguaaacggcgggaguaacuaugacucucuuaagguagccaaaug
ccucgucaucuaauuagugacgcgcaugaauggaugaacgagauucccac
ugucccuaccuacuauccagcgaaaccacagccaagggaacgggcuuggc
ggaaucagcggggaaagaagacccuguugagcuugacucuagucuggcac
ggugaagaguuuucacugacccggugaggcgggggggcgagccgggcucu
cgcuucuggcgccaagcgcccggccgcgcgccggccgggcgcgacccgcu
ccggggacagugccagguggggaguuugacuggggcgguacaccugucaa
acgguaacgcagguguccuaaggcgagcucagggaggacagaaaccuccc
guggagcagaagggcaaaagcucgcuugaucuugauuuucaguacgaaua
cagaccgugaaagcggggccucacgauccuucugaccuuuuggguuuuaa
gcaggaggugucagaaaaguuaccacagggauaacuggcuuguggcggcc
aagcguucauagcgacgucgcuuuuugauccuucgaugucggcucuuccu
aucauugugaagcagaauucaccaagcguuggauuguucacccacuaaua
gggaacgugagcuggguuuagaccgucgugagacagguuaguuuuacccu
acugaugauguguuguugccaugguaauccugcucaguacgagaggaacc
gcagguucagacauuugguguaugugcuuggcugaggagccaauggggcg
aagcuaccaucugugggauuaugacugaacgccucuaagucagaaucccg
cccaggcggaacgauacggcagcgccgcggagccucgguuggccucggau
agccggucccccgccgggguccggugcggagugcccuucguccugggaaa
cggggcgcggccggagaggcggccgcccccucgcccgucacgcaccgcac
guucguggggaaccuggcgcuaaaccauucguagacgaccugcuucuggg
ucgggguuucguacguagcagagcagcuucgcugcgaucuauugaaaguc
agcccucgacacaaggguuugu

.........................<<<<<<.....<......>......
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>>>>>>..[[[...<<<<...<<<<<<<<<<.....>>>>>>.>>>>...
..>>>>...<<<<<<<<<<...<<<<<<<<........<<<<<<<<....
<<<<......>>>>.....((......<<<.....))>>>...>.>>>>>
>>...>>>>>>>>..>>>>>>>>>>.......<<<<<<<........>>>
>>>>.....(((((..<<<<<<)))))..]]]..................
................<<....>>...............<<<<....>>>
>..................................<<<<<<<<..<<<.<
<...<<<<<<<<.<<<.....<<<<<<<<<<<..<<<<<<<.......<.
....<<<<.<<<..<<......>>>>>>>>>.>......>>>>...>>>>
>>>>>>>>>>......>>>.>>>.>>>>>...<<<<<<<.<<<<<<<<<<
.<<<<...<<..<<<....<<<<......<<<<<<<....>>>>>>>...
....>>>>...>>>........>>>>>>...>>>>>>>>>>.........
...........<<<<<..<<<<<<<...>>>>>>>.<<<<<<.<<<<.<<
<<<<<<<<..<<<<<.<<<<..<...........>...>>>>.>>>>>..
.>>>>>>>.>>>...>>>.>.>>>>.>>..<<<<<<<<<<..>>>>>>>>
.>>.....>>>>>......>>.>>>>>...>>>>>...>>>>>>>>..<<
<<........>>>><<<<<<....<<<<<<<<<<<<<..<<<...<<<<.
.......>>>>.....>>>...<<....>>...<<<<<<<......>>>>
>>>..<<<<<...<<<..<<<..[[[..((((>>>..>>>....>>>>>.
<<<<<<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>>>>
>..<<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....
>>>>>>>.....>>>...<<<..<<.<<........>>>>.>>>....>>
>>>>>>>...<<.......>>......>>>>>>...>..>.>>..<<<<<
<....<.<<<....>>>.><<.<<<<<<<...................<<
<.<<<<<<<<<...<<<....<<<..<.<<<<....<.....>..>>>>.
>..>>>..>.>>...>>>>>>>...<<<<<<<<<.........>>>>>>>
>>....>>>>>>>>>>>>..>>..<<<<.<<<<<.....>>>>>.>>>>.
...<<<......>>>...<<<<<<<...<<<(((.......<<<<<....
.<<..........<<<.<<<<<<<<<<.......<<<<............
..>>>>...>>>>>>>>>>>>>..........))).>>...>>>>>..>>
>...>>>>>>><<<<<<.<<<..........))))..]]]..........
................>>>>>>>>>....>>>>>>.<<<<<<<<<<.<<.
.....<<<<.<<<<....>>>>>>>>...>>>>>>>>>>.>>.....>>>
>>><<<....<<.....<<<<<<.......>>>>>>.<<<.....<<<<<
.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<..
..<<<....>>>..........<<.<<<<.......>>>>>>......>>
....>>>..<<<<<........>>>>>))<<<<....<<<<<..<<<<<<
<<....>>>>>>>>..<<<....>>>...<..<<<<<<<...<<<<<<<<
<<<<.<<<<<<<<<<..{{{..>>>>>>....<<<<<.<<<<.<<<<...
..<<<.....>>>.....>>>>>>>>..>>>>>.....<<<<<<<.....
..>>>>>>>>>>>...>>>>>>>>>>>>.........>>>>>>>..>...
.......>>.>>>.>>>>.>>>..>>.....<<......>>>>>>>.<<<
<.....>>>>..>>>....<<<<.....<<<<<<<.<<.........<..
...<<<<<....<<..>>.....>>>>><<.<<<<<<<<<<<........
..........>>>>>..>>>>>>.>>............<<<<<<<<<..<
<<.........>>>..<<<<<<<....<<<<.....>>>>......>>>>
>>>...<<..<<<..<<<<<<....<<<.....<<<<....>>>>.....
>>>.>>>>..>>>>><<<<<<.......>>>>>>........<.......
....<<<<<.......>>>>>.....>.....>>....>>.>>>>>>>..
>..>>.>>>>>>>..>>>>>>...>>>....<<<<<........>>>>>.
...[[[[[..{{{.{{......[[[[.{{[[[[..<<<<<.<<<<<<<<<
.<<<..<...>..>>>...<<<<<.....<<<<..<.............>
...............<<<<<<<<<<.......>>>>>>>>>>..>>>>..
>>>>>....>>>>>>>>>..>>>>>...<<<.....>>>..<<<<<<...
........>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>
.....<<.[.(((>>....>>>>>>>...<<<..<<<<.......>>>>.
.>>><<<<....>>>>>>)))]......((((((..(((..>>>>>>.))
)))))))..........]]]]..}}]]]].......<<.<...<<<<<<.
..<<.......>>...>>>>>>....>.>.>.......<<<<<.<<<<<<
..<.<.<<<<<......>>>>>...>.>.<<<.........>>>.....>
>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....}}..}}}
.]]]]].....<<<<<<.<..<<<..<.<<<<<.................
>>>>>.><<<<....<<<<....<<.<<<<<<<}}}.>>>>>..>>.>>.
..>>>>...>>>><<<<<...<<<<<...........>>>>>...>>>>>
.>>>...>.>>>>>>......<<<<<<.<<..<<<<....<<<.......
.<<<<<.<<<<....>>>>.>>>>>.......>>>...<<<..<<<<...
.<<<<.<<<<<<<......>>>>>>>>>>>...>>>>..>>>........
......>>>>..>>>>>>>>.<<<<<....................<<.<
<<<<<<.<<.....<<<<....<<<<<...>>>>>...>>>>....>>..
..>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7qvp A distinct mammalian disome collision interface harbors K63-linked polyubiquitination of uS10 to trigger hRQT-mediated subunit dissociation.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): R4 K13 H15 K23 R25 H26 S27 R28 R30 G31 R32 L33 T34 R41 Y53 K67 R68 K71 K74 R76 G78 T79 H80 R82 R85 K86
• Binding residue (residue number reindexed from 1): R3 K12 H14 K22 R24 H25 S26 R27 R29 G30 R31 L32 T33 R40 Y52 K66 R67 K70 K73 R75 G77 T78 H79 R81 R84 K85

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0016020 membrane
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0045202 synapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7qvp, PDBe:7qvp, PDBj:7qvp
• PDBsum: 7qvp
• PubMed: 36302773
• UniProt: Q9Y3U8|RL36_HUMAN Large ribosomal subunit protein eL36 (Gene Name=RPL36)