Structure of PDB 3jai Chain MM Binding Site BS01

Receptor Information

>3jai Chain MM (length=124) Species: 9986 (Oryctolagus cuniculus)

GGVMDVNTALQEVLKTALIHDGLARGIREAAKALDKRQAHLCVLASNCDE
PMYVKLVEALCAEHQINLIKVDDNKKLGEWVGLCKIDREGKPRKVVGCSC
VVVKDYGKESQAKDVIEEYFKCKK

Ligand information

>3jai Chain 9 (length=1719)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgccacuuggauaacugugguaauuc
uagagcuaauacaugccgacgggcgcugacccccuucgcgggggggaugc
gugcauuuaucaguggugacucuagauaaccucgggccgaucgcacgccc
cccguggcggcgacgaccauucgaacgucugcccuaucaacuuucgaugg
uagucgccgugccuaccauggugaccacgggugacggggaaucaggguuc
gauuccggagagggagccugagaaacggcuaccacauccaaggaaggcag
caggcgcgcaaauuacccacucccgacccggggagguagugacgaaaaau
aacaauacaggacucuuucgaggcccuguaauuggaaugaguccacuuua
aauccuuuaacgaggauccauuggagggcaagucuggugccagcagccgc
gguaauuccagcuccaauagcguauauuaaaguugcugcaguuaaaaagc
ucguaguuggaucuugggagcgggcguccccgccgccucucggccggggc
ccgaagcguuuacuuugaaaaaauuagaguguucaaagcaggcccgagcc
gccuggauaccgcagcuaggaauaauggaauaggaccgcgguucuauuuu
guugguuuucggaacugaggccaugauuaagagggacggccgggggcauu
cguauugcgccgcuagaggugaaauucuuggaccggcgcaagacggacca
gagcgaaagcauuugccaagaauguuuucauuaaucaagaacgaaagucg
gagguucgaagacgaucagauaccgucguaguuccgaccauaaacgaugc
cgaccggcgaugcggcggcguuauucccaugacccgccgggcagcuuccg
ggaaaccaaagucuuuggguuccggggggaguaugguugcaaagcugaaa
cuuaaaggaauugacggaagggcaccaccaggaguggagccugcggcuua
auuugacucaacacgggaaaccucacccggcccggacacggacaggauug
acagauugauagcucuuucucgauuccgugggugguggugcauggccguu
cuuaguugguggagcgauuugucugguuaauuccgauaacgaacgagacu
cuggcaugcuaacuaguuacgcgacccccgagcggucggcgucccccaac
uucuuagagggacaaguggcguucagccacccgagauugagcaauaacag
gucugugaugcccuuagauguccggggcugcacgcgcgcuacacugacug
gcucagcgugugccuacccuacgccggcaggcgcggguaacccguugaac
cccauucgugauggggaucggggauugcaauuauuccccaugaacgagga
auucccaguaagugcgggucauaagcuugcguugauuaagucccugcccu
uuguacacaccgcccgucgcuacuaccgauuggaugguuuagugaggccc
ucggaucggccccgccggggugcccuggcggagcgcugagaagacggucg
aacuugacuaucuagaggaaguaaaagucguaacaagguuuccguaggug
aaccugcggaaggaucauu

...<<<<<.[.((((>>>>><<<.<<<<<<...<.<<.........<<<.
<<<..<<....<<...<<<..........>>>..>>.>>......<<...
.....<<<.<....<<....<<<<.......<<.....<<.<<<.....>
>>.>>......>>.........<<<<...<<<<<<....>>>>>>...>>
>>....<<<<<...<......>..>>>>>...........<<<<.<<<<.
.....>>>>>>>>..>>>>...>>..>.>>>.<<<....<<<....<<<<
<<<.........>>>>>>>>>>......>>>...<<<.<<<<....>>>>
....>>>.>>.<<.<<<..........>>>.>>.<.<<....>>.>...>
>>>>>.........<<<....<<<<...>>>>..>>>......>>.>...
..<<.<<<<<<.<<<....>>>..>>>>>>..>>......<<..<.....
......>..>>.........<<<<<((......<<<<.....<<..))>>
.......>>>>.>>>>>..>>>>>>.>>>.........<.<((.....<.
<<...<<<.<<.....<<.<.<<....>>.>.>>.<.<..<<........
>.>>.>...<<<<<<.<.......<<...<.......>.<<<<.......
>>>>...>>......>.>>>..>>>.......<<....<<<<<<<.....
..........>>>>>>>...>>....>>....<<<<<<..<...<<<<..
<<<.<<<<<<<<...<<<......>>>......>>>>>>>>.>>>.....
..<<....>>...>>>>..>..>>>.>>>...>>>...>>.>....<<<<
<<<...<...<<<<.<.....>.>>>>...>>>>>>>>..........<<
<.<<.<<<..<.<<<<<<.<<<........>>>>>>>>>.>..>>>...<
<.)).>>...>>.....>>>.>.>.<<<......<<<<....>>>>....
>>>..)))).]<<<<<.<<<<<<<..<<..<<<<<..<<<.<<<<.<...
...<<........>>..........<<<<<.<....<<<<<........<
<.<<<........>>>>>.......>>>>>...<<.<<<..<<.<<<<<<
....<<<.<<<<<....>>>...<<<......>>>...>>.>>>....<<
<...<...<<<<..<<<.....<<<...<<<....>>>..>>>.....>>
>..>>>>..>.....<<<<<.....>>>>>........>>>....>>>.>
>>.....>>>>>>>.....>.>>>>>...>..>>>>.>>>.....<<.<<
<........<.......<<<.<<<<....>>>>.>>>....>........
.>>>.>>......<.....<<<<<..........>>>>>.....>.....
>>>>>.....<<<<<<<<.......>>>>>>>>......>>...>>>>>>
>>>>.>>....<..<<.<..<<<<.<<....<<<<<<<<.<<<..<<<<.
<<...<<<<<.<<<<<<<<<..>>>>>>>>>...>>>>>...>>.>>>>.
.>>>.>>>>>>>>...>>.>>>>...>.>>...>.....<<<<<<<<<..
..>>>>>>>>>........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3jai Structural basis for stop codon recognition in eukaryotes.
• Resolution: 3.65 Å
• Binding residue (original residue number in PDB): R36 D57 V104 G105
• Binding residue (residue number reindexed from 1): R28 D49 V96 G97

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation
• GO:0042274 ribosomal small subunit biogenesis
• GO:0090263 positive regulation of canonical Wnt signaling pathway

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005794 Golgi apparatus
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0032040 small-subunit processome
• GO:0043231 intracellular membrane-bounded organelle
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:3jai, PDBe:3jai, PDBj:3jai
• PDBsum: 3jai
• PubMed: 26245381
• UniProt: G1SFR8|RS12_RABIT Small ribosomal subunit protein eS12 (Gene Name=RPS12)