Structure of PDB 7pwo Chain M2 Binding Site BS01

Receptor Information

>7pwo Chain M2 (length=128) Species: 184922 (Giardia lamblia ATCC 50803)

SYHRFVEAGRLVYLGDGPDKGKLAVIVDILSTAAVLIDGPTTGVKRQVVS
LRWAMLTDQVINISEKPTTEQLKKEIEASPAIDTFMNSVWGKKIQQQRLR
AKATFEDMGKIAKLRRERKMAMKRIAMS

Ligand information

>7pwo Chain 1 (length=2448)

cggcccgaggcggcgggggcgacgggcggaacuuaagcauaucaguacgc
cccggaggagaaaccaaccgggauuccccguagcggcgagcgacgcggga
ggagcccgccccgaaggcgcgcugugggcgccgcgagggggcccgagggc
cccgcccgagagggugcaagccccguacggcgggcgaguagcgcugcuug
agcgugcagcgcgaagggaggcgcggcccuuccaaggcuaaauacgcccc
gggaccgauagcggaccaaguagcgcgagcgaacggugaaaaggacgccc
ggccgcucaaaagaccugaacccggccggcgccggcccgccggccccguc
ucgaaacacggaccgaggagccacgcgccgcggcgagcccgagggccccg
cggcggagcgagcgcgagacgccccgggcccgccgcgccccugcgggcgu
gcgcgggccgagccgcggcgcgugggcccgaaaggcggugaucuaugccc
ggcgagggcgaggccgggcgaaagccugguggaggcccgccgcggugcug
acgcgcagaucgcucgucggagccgggcaugggggcgaaagacucaucga
accgccugguagcugguugccuccgaaaugucucccaggacagccgccgc
ccgcaguugcggcccguagagcgcuggccggcggccgccccccaaacucc
gaagggccgcgccgccccgcccuggccugggcggggcgggcgaaugcggg
cggcgcgugggccccuccugguaagcaggacgggcgaggcgggacgaucc
ggacgccgggccagggugcgccgccggggccggaacggcgucggccgcgg
ccggcccggaaaauggagcgcgccggagccccggacccgcgcccggccgc
cgcgcgcggcggguaggaggccgcagaggccccgggggcgaaggcggcgc
gcaggccccgccggaccggccucuggugcagaucucggcagcaguagcgc
uacuccgcccccggaggacugagggggagacggguuccgcggcgccugca
ucuggccgcgggugacucgggccuaagcggcgggugaagaccgggaaggg
gcgugcccgcccgucgaacggggagccggcggagacuccggcaggcgcgg
gcggagacgcgccccccggcgacgcgcacggggaccgcggcgggcggcgc
cccggcccgcgaacgccccgcagcccccggacgccuugcgcggagagggg
ggcccgggggcggaccgcgcguccccggccgccccugaaaagccgggggg
cccgcgcgccguaccgaccgcacaggacuccggggucagcagccucuagc
gcgggagcgaacgcggcucagggaagucggcaagccggcuccguaaccuc
gggaaaaggaguggcucugacggggucagaacuggaacggacgcggggau
cccgacuguuuacuagaaacacagcgucgcgagggccgcacccggcgcug
gcgcgacgugauuucugcccagugccagaccgucaccgugaagcgauccg
ccgaagcccugguaaacggcgggaguaaugacucucuuaagguagccaaa
ugccucgucgggcaauuuccgacggcaugaauggaccaacgaggauccca
cugucccgagccgcgccuccgcgagccuccagccucgggaacgggcgagg
gccggccagcggggcaagaagacccuuuugagcuugacuccagcccgggc
cuguggggcggggccgccccgcucacccggucgcgcggggacccgcccgg
gcggggaguucggcuggggcggcgcgccugcuacaccggaccgcaggcgu
cccacggcgggcucagcgaggacccucccgcggagcagaagggcacaagc
ccgcccgacccgcgccccccgugccggcgcgggccgcgaaagcggggccu
accgauccuucgccgccccggccgcgggcgcggagguggcagaaaaguua
ccacagggauaacuggcuuguggccgccgagcgcccgcagcgacgcggcu
uuuugauccuucgaugucggcucuuccuaccguccgcgcgcaccggcgcg
gaagcgucggauuguucacccguucaagggaucgugagcuggguuuagac
cgucgugagacgguuaguuuuacccuacuggccccggggccagagcacgg
cgggccaguacggaggaacgcccgccgcgggcgcccagccccgcgguugc
ccgccggggcagaccgcgcgcccgggcccgggggccuggcgcugccgccu
cuaaagcgccaccccccccuccggccccgccgcccgcgccccagccccgu
gcccccugcccgaggcggcccccgcccgggaggaccacccggcgcggcgc
cccuguacggcgagggcgcguucgcccggggggcgcgccgcaggcgcg

......................<<<<<<.....<......>......>>>
>>.>.........<<....>>..<<<<<....<<.....>>.....>>>>
>..[[[...<<<<...........>>>><<<<..<..<<<<<......>>
>>>((......<<<.....))>>>...>>>>>........<<<<<<<...
.....>>>>>>>...(((((..<<<<<<)))))..]]]............
......................<<....>>................<<<.
>>>...........................<<<<<<..>>>>>>.<<<<.
.......>>>>.<<<<<....<<<<<<<<<<<<<..<<<...<<<.>>>.
.>>>...<<....>>............<<<<<<<<<<<<<....>>>>>>
>.>>>>>>...>>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<
<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<..<<.<
<........>>>>.>>>....>>>>>>>>>...<<.......>>......
>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<
<<<<<.<<<<<<<<<...<<<...<<<..<<<<..>>>>..>>>..>>.>
...>>>>>>>.<<<<<<<<<<<......>>>>>>>>>>>...>>>>>>>>
>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>
>...<<<<<<<..<<<<(((...<<<<<<<.....<<<.<<<<<<<..>>
>>>>>>>>..........)))......>>>>>>>>>>>..>>>>>>><<<
<<<..>>>>>>....>>>>>><.<<<<<<<<...<<......<<<<<.<<
....>>.>>>>>...>>>>>>>>>>..>.....>>>>>.<<.....<<..
..<<<<<....>>>>>..<<.....<<<<..<<...<<<<<<...<....
...>.>>>>>>....((<<<.<<...<<<...<<<....>>>......<<
<<..>>>>...>>.>....>>...<<<<<........>>>>>))<<<<<<
<<<....>>><<<<<<<<<<...<<<..<<<<<<<<<<<<<<<<<.....
....>>>>>....<<<<<<...<<<<<<....<<.....>>......>>>
>>>...>>>>>>..>>>>>.>>>>>>>..>>>.........>>>>>>>>>
>>>>>>>.>>>..>>..<<.....>>.>>>>.<<<<.....>>>>..>>.
...<<<<....<<<<<<<<.<<.........<.....<<<<<....<<..
>>.....>>>>><<.<<<<<<...>>>>>>.>>............<<<<<
<<<<..<<<.........>>>..<<<<<<<....<<<<....>>>>....
..>>>>>>>...<<..<<<..<<<<<<<...<<....<<<.<....>.>>
>....>>.>>>>>..>>>>><<<<<<....>>>>>>........<.....
......<<<<<<.....>>>>>>....>.....>>....>>.>>>>>>>.
.>..>>.>>>>>>>>.>>>>>>....>>....<<<<<........>>>>>
...((((((..{{{.........{{{..{{[[[[..<<<<<.<<<<<<<<
..<<<<<<<<<..>>>>>>>>>...<<<<......>>>>.....>>>>>>
>>..>>>>>...<<<.....>>>..<<<<<<...........>>>>>>..
<<...<<<<<<<...<.<<<<..>>>>.>.....<<...(((>>....>>
>>>>>...<<<<<<<<.........>>>>>>>>.<<<....>>>.>>)))
.......((((((.((((.>>>>>>))))))))))..........]]]].
.}}.}}}.......<<.<.<.<<<<<<...<<.......>>...>>>>>>
...>>.>.>.......<<<<<.<<<<<<..<<<<<<<<<......>>>>>
>..>>>.<<<.........>>>....>>>>>..>...>>>.>>....<<<
<<<<....>>>>>>>........}}}.))))))..<<<<<<.<<<..<<<
<<<<<..............>>>>>>>><<<<<...<.<....<<<<<<<<
<<....>>>>>.>>>>>....>.>.>>>>>.<<<...<<<<<<.......
....>>>>>>...>>>..>>>.>>>>>>...<.<<<..............
...............>>>.>...<<.<<<<.......>>>>..<<<<<<<
<<<<....<<<<<<.<..>.>>>>>>...>>>>.>>>>>>>....>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7pwo Cryo-EM structure of the ancient eukaryotic ribosome from the human parasite Giardia lamblia.
• Resolution: 2.75 Å
• Binding residue (original residue number in PDB): S2 Y3 H4 S51 R53 W54 W91 K94 R116 R117 K120
• Binding residue (residue number reindexed from 1): S1 Y2 H3 S50 R52 W53 W90 K93 R115 R116 K119

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7pwo, PDBe:7pwo, PDBj:7pwo
• PDBsum: 7pwo
• PubMed: 35100413
• UniProt: A8B4F8