Structure of PDB 8jch Chain M Binding Site BS01

Receptor Information
>8jch Chain M (length=721) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
VPSFFRWLSRKYPKIISPVLEEQPQIVDGVILPLDYSASNPNGELDNLYL
DMNGIVHPCSHPENKPPPETEDEMLLAVFEYTNRVLNMARPRKVLVMAVD
GVAPRAKMNQQRARRFRSARDAQIENEAREEIMRQRDAVRNKKTWDSNAI
TPGTPFMDKLAAALRYWTAFKLATDPGWKNLQVIISDATVPGEGEHKIMN
FIRSQRADPEYNPNTTHCIYGLDADLIFLGLATHEPHFKILREDVFAQDN
RKREEEKQFLQKQNSEQPFLWLHINVLREYLSAELWVPGLPFTFDLERAI
DDWVFMCFFCGNDFLPHLPCLDVRENSIDILLDIWKVVLPKLKTYMTCDG
VLNLPSVETLLQHLGSREGDIFKTRHIQEARKKEEFVKLFEPGYHERYYT
AKFHVTPQDIEQLRKDMVKCYIEGVAWVLMYYYQGCASWNWFYPYHYAPL
ATDFHGFSHLEIKFEEGTPFLPYEQLMSVLPAASGHALPKIFRSLMSEPD
SEIIDFYPEEFPIDMNGKKMSWQGIALLPFIDQDRLLTAVRAQYPLLSDA
ERARNIRGEPVLLISNKNANYERFSKKLYSKENNNNNVVVKFQHFKSGLS
GIVSKDVEGFELNGKIVCPIQGGSLPNLSTTLILKMSYRLIPLPSRNKSI
ILNGFIPSEPVLTAYDLDSIMYKYNNQNYSRRWNFGNDLKQNIVPVGPKG
ITQYKPRTGGYRAFFYFAELS
Ligand information
>8jch Chain P (length=23) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
acagaucguguccaucgagaggu
.......................
Receptor-Ligand Complex Structure
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PDB8jch Structural basis of exoribonuclease-mediated mRNA transcription termination
Resolution2.7 Å
Binding residue
(original residue number in PDB)		V3 P4 S5 R8 R12 N55 H59 Q113 R116 R117 S157 L232 D233 K402 K729 W732 N886 Q887 S890 R891 W893
Binding residue
(residue number reindexed from 1)		V1 P2 S3 R6 R10 N53 H57 Q111 R114 R115 S147 L222 D223 K382 K519 W522 N676 Q677 S680 R681 W683
Enzymatic activity
Enzyme Commision number 3.1.13.-
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0003729 mRNA binding
GO:0004527 exonuclease activity
GO:0004534 5'-3' RNA exonuclease activity
GO:0005515 protein binding
GO:0019843 rRNA binding
Biological Process
GO:0000398 mRNA splicing, via spliceosome
GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0000956 nuclear-transcribed mRNA catabolic process
GO:0006139 nucleobase-containing compound metabolic process
GO:0006353 DNA-templated transcription termination
GO:0006364 rRNA processing
GO:0006397 mRNA processing
GO:0030846 termination of RNA polymerase II transcription, poly(A)-coupled
GO:0030847 termination of RNA polymerase II transcription, exosome-dependent
GO:0034244 negative regulation of transcription elongation by RNA polymerase II
GO:0043144 sno(s)RNA processing
GO:0071028 nuclear mRNA surveillance
GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process
GO:0110155 NAD-cap decapping
GO:1904595 positive regulation of termination of RNA polymerase II transcription
Cellular Component
GO:0005634 nucleus
GO:0005739 mitochondrion
GO:0090730 Las1 complex
GO:0110103 RNA polymerase II termination complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8jch, PDBe:8jch, PDBj:8jch
PDBsum8jch
PubMed38538796
UniProtQ02792|XRN2_YEAST 5'-3' exoribonuclease 2 (Gene Name=RAT1)

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