Structure of PDB 7wu0 Chain M Binding Site BS01

Receptor Information

>7wu0 Chain M (length=123) Species: 9606 (Homo sapiens)

GVMDVNTALQEVLKTALIHDGLARGIREAAKALDKRQAHLCVLASNCDEP
MYVKLVEALCAEHQINLIKVDDNKKLGEWVGLCKIDREGKPRKVVGCSCV
VVKDYGKESQAKDVIEEYFKCKK

Ligand information

>7wu0 Chain 2 (length=1624)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucguacuuggauaacugugguaa
uucuagagcuaauacaugccgacgggcgcugacccccuucgcggggggga
ugcgugcauuuaucaguggugacucuagauaaccucgggccgaucgcacg
ccggcggcgacgacccauucgaacgucugcccuaucaacuuucgauggua
gucgccgugccuaccauggugaccacgggugacggggaaucaggguucga
uuccggagagggagccugagaaacggcuaccacauccaaggaaggcagca
ggcgcgcaaauuacccacucccgacccggggagguagugacgaaaaauaa
caauacaggacucuuucgaggcccuguaauuggaaugaguccacuuuaaa
uccuuuaacgaggauccauuggagggcaagucuggugccagcagccgcgg
uaauuccagcuccaauagcguauauuaaaguugcugcaguuaaaaagcuc
guaguuggaucucucucggccgaagcguuuacuuugaaaaaauuagagug
uucaaagcaggccgccuggauaccgcagcuaggaauaauggaauaggacc
gcgguucuauuuuguugguuuucggaacugaggccaugauuaagagggac
ggccgggggcauucguauugcgccgcuagaggugaaauucuuggaccggc
gcaagacggaccagagcgaaagcauuugccaagaauguuuucauuaauca
agaacgaaagucggagguucgaagacgaucagauaccgucguaguuccga
ccauaaacgaugccgaccggcgaugcggcggcguuauucccaugacccgc
cgggcagcuuccgggaaaccaaagucuuuggguuccggggggaguauggu
ugcaaagcugaaacuuaaaggaauugacggaagggcaccaccaggagugg
agccugcggcuuaauuugacucaacacgggaaaccucacccggcccggac
acggacaggauugacagauugauagcucuuucucgauuccguggguggug
gugcauggccguuguugguggagcgauuugucugguuaauuccgauaacg
aacgacucuggcaugcuauucuuagagggacaaguggcguucagccaccc
gagauugagcuaacaggucugugaugcccuuagauguccggggcugcacg
cgcgcuacacugacuggcucagcguguccuacgccggcaggcgcggguaa
cccguugaaccccauucgugauggggaucggggauugcaauuauucccca
ugaacgaggaauucccaguaagugcgggucauaagcuugcguugauuaag
ucccugcccuuuguacaccgcgucgcuacuaccgauuggaugguuuagug
aggcccucggaucggccccgccggggugcccuggcggagcgcugagaaga
cggucgaacuugacuaucuagaggaaguaaaagucggguuuccguaggug
aaccugcggaaggaucauuaacgg

...<<<<<.[.((((>>>>><<<.<<<<<<...<.<<..<......<<<.
<<<..<<....<<....<<..........>>...>>.>>......<<...
.....<<<.<....<<....<<<<<.........<<.....<<.<<<...
..>>>.>>......>>.........<<<<...<<<<<<....>>>>>>..
.>>>><<..<<<<<...<......>..>>>>>......>>...<<<<.<<
<..>>>>>>>.>>.>>>...>>..>.>>>.<<<....<<<....<<<<<<
<.........>>>>>>>>>>......>>>...<<<.<<<<....>>>>..
..>>>.>>.<<.<<<..........>>>.>>.<.<<<..>>>.>...>>>
>>>.........<<<....<<<<...>>>>..>>>..>...>>.>.....
<<<<<<.<<.<<<....>>>.>>..>>>.>>>......<<..<.......
....>..>>.........<<<<<((......<<<<.....<<..))>>..
.....>>>>.>>>>>..>>>>>>.>>>.........<.<((.....<.<<
...<<<.<....<<.<<<..>>>>>...<<<<<<.<.......<<...<.
......>.<<<<.>>>>...>>......>.>>>..>>>........<.<<
.<<<<<<<...............>>>>>>>.>>.>.....>....<<<<<
<..<...<<<<..<<<.<<<<<<<<...<<<......>>>......>>>>
>>>>.>>>.......<<....>>...>>>>..>..>>>.>>>...>>>..
.>>.>....<<<<<<<...<...<<<<.........>>>>...>>>>>>>
>..........<<<.<<.<<<..<.<<<<<<.<<<<......>>>>>>>>
>>.>..>>>...<<..))>>...>>.....>>>.>.>.<<<......<<<
<....>>>>....>>>..)))).]..<<<.<<<<<<<..<<..<<<<<..
<<<.<<<<<<............................<<<<<.<....<
<<<<<.......<<.<<<........>>>.>>.....>>>>>>......<
<<<<<<<<<<<<.<<<.<<<<<....>>>...<<<......>>>...>>.
>>>..<<<...<...<<<....>>>..>.....<<<<<.....>>>>>..
......>>>...>>.>>>>.>>>.>>>>.......>.>>>>>...>>.>>
>>.>>>.....<<.<<<........<.<<<.<<<<....>>>>.>>>...
.>.........>>>.>>......<.....<<<<<..........>>>>>.
....>.....>>>>>.....<<<<<<<<.......>>>>>>>>......>
>...>>>>>>>>>>..<<<.<<.<..<<<<.<<....<<<<<<<<.<<<.
.<<<<.<<...<<<<<<<<<<<<<<<..>>>>>>>>>.>.>>>>>...>>
.>>>>..>>>.>>>>>>>>...>>.>>>>...>.>>>>><<<<<<<<<..
..>>>>>>>>>.............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7wu0 The nucleoplasmic phase of pre-40S formation prior to nuclear export.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): R33 G34 I35 R36 E37 K40 D57 L91 K102 V104 G105 C106 S107
• Binding residue (residue number reindexed from 1): R24 G25 I26 R27 E28 K31 D48 L82 K93 V95 G96 C97 S98

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042274 ribosomal small subunit biogenesis
• GO:0090263 positive regulation of canonical Wnt signaling pathway
• GO:1990145 maintenance of translational fidelity

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005794 Golgi apparatus
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0016020 membrane
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0032040 small-subunit processome
• GO:0043231 intracellular membrane-bounded organelle
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7wu0, PDBe:7wu0, PDBj:7wu0
• PDBsum: 7wu0
• PubMed: 36321656
• UniProt: P25398|RS12_HUMAN Small ribosomal subunit protein eS12 (Gene Name=RPS12)