Structure of PDB 7uw1 Chain M Binding Site BS01

Receptor Information

>7uw1 Chain M (length=119) Species: 480119 (Acinetobacter baumannii AB0057)

MRHRNSGVKLGRTSSHRKAMFENLANSLFEHELIKTTLPKAKELRRVAEP
LITLAKNDTVANRRLAFARTRNAATVGKLFTVLGPRYKERNGGYLRVLKA
GFRAGDAAPMAYVELVDRE

Ligand information

>7uw1 Chain A (length=2726)

gucaaguaauuaagugcaugugguggaugccuuggcagucagaggcgaug
aaagacgugauagccugcgaaaagcuccggggaggcggcaaauauccuuu
gauccggagaugucugaaugggggaacccaccuacuuuaagguagguauu
gcaacaugaauacauaguguugcaaggcgaacgaggggaagugaaacauc
ucaguacccuuaggaaaagaaaucaauugagauucccucaguagcggcga
gcgaacggggaucagcccauuaaguuauguguguuuuaguggaacgcucu
gggaagugcgaacguagagggugauauucccguacacgaaagggcacaca
uaaugaugacgaguagggcgaggcacgugaaaccuugucugaauaugggg
ggaccauccuccaaggcuaaauacuccugacugaccgauagugaaccagu
accgugagggaaaggcgaaaagaaccccugugaggggagugaaauagauc
cugaaaccgcaugcauacaagcagugggagcaccuucguggugugacugc
guaccuuuuguauaaugggucagcgacuuauauucaguagcgagguuaac
cguauaggggagccguagagaaaucgagucuuaauagggcguuuaguugc
uggguauagacccgaaaccaggcgaucuauccaugagcagguugaagguu
ggguaacacuaacuggaggaccgaacccacugucguugaaaagccagggg
augacuuguggauaggggugaaaggcuaaucaagccuggugauagcuggu
ucuccccgaaagcuauuuagguagcgccucggacgaauaccauagggggu
agagcacuguuucggcaccgaugcaaacuccgaauaccuaugaguacuau
ccgggagacagacugcgggugcuaacguccguagucaagaggaaaacaau
ccagaccgccagcuaaggccccaaaaucauaguuaagugggaaacgaugu
gggaaggcauagacagcuauagucgagucggccugcgcggaagauguaac
ggggcuaaaacuaugugccgaagcugcggauguauacuuuguauacgugg
uaggggagcguucuguaagccgaugaagguguguugagaagcaugcugga
gguaucagaagugcgaaugcugacgugaguaacgacaaaacgggugaaaa
acccguucgccgaaagaccaaggguuccaguccaacguuaaucggggcug
ggugagucgaccccuaaggcgaggccgaaaggcguagucgaugggaaaau
gguuaauauuccauuacuucuguguaaugcgaugagaggacggagaaggc
uaaaucagccuggcguugguuguccaggugaaaggauguaggcauguauc
uuaggcaaauccgggguacucuaugcugagaucugauagcaagcuguacu
ugugcagcgaagugguugaugccaugcuuccaggaaaagucucuaagcuu
caguuacacaggaaucguacccgaaaccgacacagguggucaggucgagu
agaccaaggcgcuugagagaacucugcugaaggaacuaggcaaaauggua
ccguaacuucgggagaagguacgcuguuguuggugauggaacucgcuucc
ugagcugacgacagccgcagaaaccaggccgcugcaacuguuuauuaaaa
acauagcacucugcaaacacgaaaguggacguauagggugugaugccugc
ccggugcuggaagguuaauugaugggguuagcguaagcgaagcucuugau
cgaagccccaguaaacggcggccguaacuauaacgguccuaagguagcga
aauuccuugucggguaaguuccgaccugcacgaauggcauaaugauggcg
gcgcugucuccagcagaggcucagugaaaucgaaaucgcugugaagaugc
aguguacccgcggcuagacggaaagaccccgugaaccuuuacugcagcuu
gacacugaacuuugagguucuaacucugucccgugauccgggacgaggac
cgugucugguggguaguuugacuggggcggucuccuccuaaagaguaacg
gaggaguacgaaggugcgcucagcguggucggaaaucacgcguagaguau
aaaggcaaaagcgcgcuuaacugcgagacccacaagucgagcagguacga
aaguaggucuuagugauccggugguucuguauggaagggccaucgcucaa
cggauaaaagguacucuggggauaacaggcugauaccgcccaagaguuca
uaucgacggcgguguuuggcaccucgaugucggcucaucucauccugggg
cugaagcaggucccaaggguauggcuguucgccauuuaaagagguacgcg
agcuggguuuagaacgucgugagacaguucggucccuaucuaccgugggc
gcuggaaauuugagaggaucugcuccuaguacgagaggaccagaguggac
gaaccucugguguaccgguugugacgccagucgcaucgccggguagcuau
guucggaagggauaaccgcugaaagcaucuaagcgggaagccuaccucaa
gauaagauuucccuaggacuuuauguccucuaaagagccguucgagacua
ggacguugauagguuggauguggaagcauagugauaugugaagcugacca
auacuaauugcucgugaggcuugacu

<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<....
..<<<<<<.[..(((.>>>.....<<<<<<<..<<.)))...]..>>...
..>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<<
<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>>
......>>>>>.>.........<<<..>>>...<<<<<.....<<.....
>>....>>>>>...<<<......<<<<<<<<<<<<<<.<<<...<<<.<.
.....>.>>>((......<<<.....))>>>...>>>...>>>>>>>>>>
>>>>...........<<<<<<<<.........>>>>>>>>......<<<<
<..<<<<>>>>>..>>>......]].>>>>>>>.]]..............
.<<....>>....]].........<<<<.....>>>>.............
.....>>>>>>>>>>>.......<<<<..<<<<<.....>>>>>..>>>>
...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...<
<.....>>...>>>...<<....>>..<<<<.....>>>>.....>>>>>
>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<<...<<<<
<<......>>>>>>.>...>>>...<<<.<<<.<........>.>>>>>>
....>>>>>>>>>...<<<.....>>>......>>>>>>...>..>.>>.
.<<<<<<....<<<<<....>>>>>.<<<<<<<....<<<<<<<<<....
.<<<...<<<.<<<<..>>>>.>>>..>>.>.....>>>>>>.>>>...>
>>>>>.>...<<<<<<<<<<......>>>>>>>>>>....<<<......>
>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..<<
<<<..<<........<<<..>>>....>>..>>>>>.>>...........
)))......>>>>>>.>>>...>>>>>>><<<<<<<<...>>>>>>>>..
..>>>>>>..<<<<<...<<<......<<<<<<<<....>>>>>>>>...
>>>..>>>>>........>>>>>><......<<.....<<<<<<......
.>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>>
>....((<<<.<<<...<<<...<<<....>>>...>>>...>>>..<<<
<<........>>>>>))<<<<<<<<<<<<<...<<<<....<<<<..<<<
..<<<<<<<<<<<...((((...>>>>>>....<<<<...<<<<.<<<<<
<..<<.....>>.>>>>>>....>>>>...>>>>........<<<<<...
...>>>>>....>>>>>...>>>...>>>>.........>>>>...>>..
..>>>>>>>>>>>.>>>..>>.....<<......>>>>>>>.<<<<....
.>>>>..>>>>..<<<<....<<<<<<<<.<<...............<<<
<<.....<..>......>>>>><<<<<<<<<<<<...<<<<.....>>>>
...>>>>>>>>>>>>............<<<<<<<<<..<<<.........
>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<<
<..<<<<<<<..<<<<.......<<<<<..<<....>>...>>>>>....
...>>>>>>>>>..>>>>><<<<<<.......>>>>>>........<...
........<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>>>
>>.....>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>
>>>>..<<<<<<<.<<<<.[[......<<<<.[[<<<<..<<<<..<<<.
<<<<<.<<<<<<..>>>>>>...<<<.<<<<<<.....>>>>>>>>>...
.>>>>>.>>>...>>>>..<<<<.....>>>><<<<<<...........>
>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[
.(((>>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<<..
..>>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>.....
........>>>>..]]>>>>.......<<<<...<<<<<<...<<.....
..>>...>>>>>>....>>>.>.......<<<<<.<<<<<<..<<<<<<<
<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>..
.>>>.>>....<<<<<<<....>>>.>>>>....]]..>>>>>>>>>>>.
...<<<<<<<...<<<<..<<<<<<.................>>>>>>.<
<<<<...<<<<...<<<<<<<<<<<))))>>>>>>..>>>>>...>>>>.
>>>>>...<<<....<<<<<...........>>>>>....>>>.>>>>..
.....>>>>>>>..<<<<<.....>>>>><....<<<<<<<<<.......
>>>>>....<<<<<<<<..<<....<<<<<....>>>>>...>>...>>>
>>.>>>...>>>>..>..>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7uw1 Streptothricin F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosome.
• Resolution: 2.21 Å
• Binding residue (original residue number in PDB): M1 R2 H3 R4 N5 S6 G7 K9 L10 R12 H16 K18 N23 L24 H31 I34 K35 T36 T37 P39 K42 R46 E49 T53 R63 R64 F67 A68 R69 R71 A74 R90 N91 G92 G93 R96 K99 R103 G105 D106 A107
• Binding residue (residue number reindexed from 1): M1 R2 H3 R4 N5 S6 G7 K9 L10 R12 H16 K18 N23 L24 H31 I34 K35 T36 T37 P39 K42 R46 E49 T53 R63 R64 F67 A68 R69 R71 A74 R90 N91 G92 G93 R96 K99 R103 G105 D106 A107

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7uw1, PDBe:7uw1, PDBj:7uw1
• PDBsum: 7uw1
• PubMed: 37192172
• UniProt: B7IA13|RL17_ACIB5 Large ribosomal subunit protein bL17 (Gene Name=rplQ)