Structure of PDB 7r4x Chain M Binding Site BS01

Receptor Information

>7r4x Chain M (length=104) Species: 9606 (Homo sapiens)

VNTALQEVLKTALIHDGLARGIREAAKALDKRQAHLCVLASNCDEPMYVK
LVEALCAEHQINLIKVDDNKKLGEWVGLCKVVGCSCVVVKDYGKESQAKD
VIEE

Ligand information

>7r4x Chain 2 (length=1646)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucgcacuuggauaacugugguaa
uucuagagcuaauacaugccgacgggcgcugaccccgggggaugcgugca
uuuaucaguggugacucuagauaaccucgggccgaucgcacgcccggcgg
cgacgacccauucgaacgucugcccuaucaacuuucgaugguagucgccg
ugccuaccauggugaccacgggugacggggaaucaggguucgauuccgga
gagggagccugagaaacggcuaccacauccaaggaaggcagcaggcgcgc
aaauuacccacucccgacccggggagguagugacgaaaaauaacaauaca
ggacucuuucgaggcccuguaauuggaaugaguccacuuuaaauccuucc
acgaggauccauuggagggcaagucuggugccagcagccgcgguaauucc
agcuccaauagcguauauuaaaguugcugcaguuaaaaagcucguaguug
gaucuugggagcgggcguccccgccuuacuuugaaaaaauuagaguguuc
aaagcaggccgccuggauaccgcagcuaggaauaauggaauaggaccggc
caugauuaagagggacggccgggggcauucguauugcgccgcuagaggug
aaauucuuggaccggcgcaagacggaccagagcgaaagcauuugccaaga
auguuuucauuaaucaagaacgaaagucggagguucgaagacgaucagau
accgucguaguuccgaccauaaacgaugccgacuggcgaugcggcggcgu
uauucccaugacccgccgggcagcuuccgggaaaccaaagucuuuggguu
ccggggggaguaugguugcaaagcugaaacuuaaaggaauugacggaagg
gcaccaccaggaguggagccugcggcuuaauuugacucaacacgggaaac
cucacccggcccggacacggacaggauugacagauugauagcucuuucuc
gauuccgugggugguggugcauggccguucuuaguugguggagcgauuug
ucugguuaauuccgauaacgaacgagacucuggcaugcuaacuaguuacg
cgaccccgcggucggcgucccccaacuucuuagagggacaaguggcguuc
agccacccgagauugagcaauaacaggucugugaugcccuuagauguccg
gggcugcacgcgcgcuacacugacuggcucagcgugugccuacccuacgc
cggcaggcgcggguaacccguugaaccccauucgugauggggaucgggga
uugcaauuauuccccaugaacgaggaauucccaguaagugcgggucauaa
gcuugcguugauuaagucccugcccuuuguacacaccgcccgucgcuacu
accgauuggaugguuuagugaggcccucggaucggccccgccggggugcc
cuggcggagcgcugagaagacggucgaacuugacuaucuagaggaaguaa
aagucguaacaagguuuccguaggugaaccugcggaaggaucauua

...<<<<<.[.((((>>>>><<<.<<<<<<...<.<<.........<<<.
<<<..<<....<<....<<..........>>...>>.>>......<<...
.....<<<.<....<<....<<<<<.........<<.....<<.<<<...
..>>>.>>......>>.........<<<<...<<<..>>>...>>>><<.
.<<<<<...<......>..>>>>>......>>...<<<<.<<<<.>>>>>
>>>.>>.>>>...>>..>.>>>.<<<....<<<....<<<<<<<......
...>>>>>>>>>>......>>>...<<<.<<<<....>>>>....>>>.>
>.<<.<<<..........>>>.>>.<.<<....>>.>...>>>>>>....
.....<<<....<<<<...>>>>..>>>......>>.>.....<<<<<<<
<..<<<....>>>...>>>>.>>>>......<<..<...........>..
>>.........<<<<<((......<<<<.....<<..))>>.......>>
>>.>>>>>..>>>>>>.>>>.........<.<((.....<.<<...<<<.
<<.......................<<<<<<.<.......<<...<....
...>.<<<<.>>>>...>>......>.>>>..>>>........<.<..>.
>....>>....<<<<<<..<...<<<<..<<..<<<<<<<<...<<<...
...>>>......>>>>>>>>..>>.......<<....>>...>>>>..>.
.>>>.>>>...>>>...>>.>....<<<<<<<...<...<<<<.<.....
>.>>>>...>>>>>>>>..........<<<.<<.<<<..<.<<<<<<.<<
<<<....>>>>>>>>>>>.>..>>>...<<..))>>...>>.....>>>.
>.>.<<<......<<<......>>>....>>>..)))).]<<<<<.<<<<
<<<..<<..<<<<<..<<<.<<<<........<<........>>......
....<<<<<.<....<<<<<<.......<<.<<<........>>>.>>..
...>>>>>>...<<.<<<..<<.<<<<<<....<<<.<<<<<....>>>.
..<<<......>>>...>>.>>>....<<<...<...<<<<..<<<<<<<
<<<<<....>>>>.>>>>.....>>>>..>>>>..>.....<<<<<....
.>>>>>........>>>....>>>.>>>.....>>>>>>>.....>.>>>
>>......>>>>.>>>.....<<.<<<........<.......<<<.<<<
<....>>>>.>>>....>.........>>>.>>............<<<<<
..........>>>>>...........>>>>>.....<<<<<<<<......
.>>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<..<<<<
.<<....<<<<<<<<.<<<..<<<<.<<...<<<<<...<<.........
...>>.....>>>>>...>>.>>>>..>>>.>>>>>>>>...>>.>>>>.
..>.>>...>.....<<<<<<<<<....>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7r4x Cryo-EM reconstruction of the human 40S ribosomal subunit at 2.15 angstrom resolution.
• Resolution: 2.15 Å
• Binding residue (original residue number in PDB): R33 G34 I35 R36 E37 K40 C56 D57 E58 Y61 V104 C106 S107
• Binding residue (residue number reindexed from 1): R20 G21 I22 R23 E24 K27 C43 D44 E45 Y48 V82 C84 S85

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042274 ribosomal small subunit biogenesis
• GO:0090263 positive regulation of canonical Wnt signaling pathway
• GO:1990145 maintenance of translational fidelity

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005794 Golgi apparatus
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0016020 membrane
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0032040 small-subunit processome
• GO:0043231 intracellular membrane-bounded organelle
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7r4x, PDBe:7r4x, PDBj:7r4x
• PDBsum: 7r4x
• PubMed: 36951107
• UniProt: P25398|RS12_HUMAN Small ribosomal subunit protein eS12 (Gene Name=RPS12)