Structure of PDB 7p7u Chain M Binding Site BS01

Receptor Information

>7p7u Chain M (length=147) Species: 1351 (Enterococcus faecalis)

MRTTYMAKAGEVERKWYVVDATDVPLGRLSAVVASVLRGKNKPTFTPHVD
TGDFVIVINADKVKLTGKKATDKIYYRHSMYPGGLKSVTAGELRDKNSRR
LIETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI

Ligand information

>7p7u Chain A (length=2824)

uuaagugaauaagggcgcacgguggaugccuuggcacuaggagccgauga
aggacgggacuaacaccgauaugcuuuggggagcuguaaguaagcuauga
uccagagauuuccgaaugggggaacccaauaucuuuuauaggauauuacu
uuucagugaauacauagcugauuagagguagacgcagagaacugaaacau
cuuaguaccugcaggaagagaaagaaaauucgauucccugaguagcggcg
agcgaaacgggaagagcccaaaccaacaagcuuguugggguuguaggacu
ccgauaugguagucuguuaguauaguugaaggauuuggaaaauuccgcua
aagagggugaaagccccguagacgaaaugcugacaacaccuaggaggauc
cugaguacggcggaacacgagaaauuccgucggaauccgcggggaccauc
ccgcaaggcuaaauacucccuagugaccgauagugaaccaguaccgugag
ggaaaggugaaaagcaccccggaaggggagugaaauagauccugaaaccg
ugugccuacaacaagucaaagcucguuaaugagugauggcgugccuuuug
uagaaugaaccggcgaguuacgauugcaugcgagguuaagucgaagagac
ggagccgcagcgaaagcgagucugaauagggcgaaugaguauguagucgu
agacccgaaaccaugugaucuacccauguccagguugaaggugcgguaaa
acgcacuggaggaccgaacccacguacguugaaaagugcggggaugaggu
guggguagcggagaaauuccaaacgaacuuggagauagcugguucucucc
gaaauagcuuuagggcuagccucggaauugagaaugauggagguagagca
cuguuuggacuaggggcccaucucggguuaccgaauucagauaaacuccg
aaugccauucauuuauauccgggagucagacugcgagugauaagauccgu
agucgaaagggaaacagcccagaccaccagcuaaggucccaaaauauaug
uuaaguggaaaaggaugugggguugcacagacaacuaggauguuggcuua
gaagcagccaccauuuaaagagugcguaauagcucacuagucgagugacc
cugcgccgaaaauguaccggggcuaaacauauuaccgaagcuguggacua
caccauuagguguagugguaggagagcguucuaagggcguugaaggucga
ucgugaggacggcuggagcgcuuagaagugagaaugccgguaugaguagc
gaaagacaggugagaauccuguccaccguaugacuaagguuuccugggga
aggcucguccgcccaggguuagucgggaccuaagccgaggccgauaggcg
uaggcgauggacaacagguugauauuccuguaccaguuguuuuuguuuga
gcaauggagggacgcaguaggcuaaggaaugcaugcgauuggaagugcau
guccaagcaaugagucuugaguagaguuaaaugcuuuacucuuuaaggac
aaguugugacggggagcgaaauaguagcgaaguuccugacgucacacugc
caagaaaagcuucuagugagaaaacaacugcccguaccguaaaccgacac
agguagucgaggagaguauccuaaggugagcgagcgaacucucguuaagg
aacucggcaaaaugaccccguaacuucgggagaaggggugcugacuucgg
ucagccgcagugaauaggcccaagcgacuguuuaucaaaaacacaggucu
cugcaaaaucguaagaugaaguauaggggcugacgccugcccggugcugg
aagguuaagaggauggguuagcuucggcgaagcucagaauugaagcccca
guaaacggcggccguaacuauaacgguccuaagguagcgaaauuccuugu
cggguaaguuccgacccgcacgaaaggcguaacgauuugggcacugucuc
aacgagagacucggugaaauuuuaguaccugugaagaugcagguuacccg
cgacaggacggaaagaccccauggagcuuuacuguaguuugauauugagu
guuuguaccuguuaugaacccucuaacccgcaccacuaaucgugguggga
gacagugucagaugggcaguuugacuggggcggucgccuccuaaaaggua
acggaggcgcccaaagguucccucagaaugguuggaaaucauucgaagag
uguaaaggcagaagggagcuugacugcgagaccuacaagucgagcaggga
cgaaagucgggcuuagugauccggugguuccgcauggaagggccaucgcu
caacggauaaaagcuacccuggggauaacaggcuuaucucccccaagagu
ccacaucgacggggagguuuggcaccucgaugucggcucgucgcauccug
gggcuguagucggucccaaggguugggcuguucgcccauuaaagcggcac
gcgagcuggguucagaacgucgugagacaguucggucccuauccgucgcg
ggcguuggaaauuugagaggagcuguccuuaguacgagaggaccgggaug
gacuuaccgcugguguaccaguuguucugccaagggcauugcuggguagc
uauguagggaagggauaaacgcugaaagcaucuaagugugaagcccaccu
caagaugagauuucccauuucuuuaagaaaguaagaccccugagagauga
ucagguagauagguuggaaguggaaggcuagugauaguuggagcggacca
auacuaaucggucgaggacuuaac

<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
..<<<<<.........>>>>>..>>.>>....<<<<<......<<....>
>.......>>>>.>.........<<....>>..<<<<<......<<....
.>>.....>>>>>..(((....<<<<<<<..>>>>>>>......<<<<<<
<<.....<<<.<<.<<<<<<<<<.<<<...<<<.<......>.>>>((..
....<<<.....))>>>...>>>...>>>>>>>>>>>>>>..>>>>..>>
>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<<<<>
>>>>..)))......]].>>>>>>>.]]...............<<....>
>....]].........<<<<....>>>>..................>>>>
>>>>>>>.......<<<<..<<<<......>>>>..>>>>...<.<....
....>.>.<<<<<<....<<<<<<<<<<<<<..<<<...<<<.....>>>
...>>>...<<....>>..<<<<.....>>>>......>>>>>>>>>>>>
>..<<.<..<<<<.<<.....<<<<<<<<<..<<<....<<<<<<.....
.>>>>>>.....>>>...<<<.<<<<<........>>>>>>>>....>>>
>>>>>>...<<<.....>>>......>>.>>>...>..>.>>..<<<<<<
....<<<<<....>>>>><<<<<<<<.<...<<<<<<<<<.....<<<..
.<<<<<<<<...<<<<<<<.....>>>>>.>>...>>>>>>>>..>>.>.
....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<.<<......>>.>>>
>>>>....<<<......>>>...<<<<.<<<...<<<(((.....<<<<<
<...........<<..<<<<<<<<<........<<<<<.<<...<<<<..
.....>>>>...>>.........<<......>>...>>>>>....>>>>>
>>>>.>>...........))).....>>>>>>.>>>...>>>>>>><<<<
<<<<....>>>>>>>>....>>>>>>..<<<<<<<.<<......<<<<<.
<<....>>.>>>>>...>>.>>>>>>>........>>>>>><......<<
....<<<<<<.......>>>>>><<<<....<<<<<.<<<..<<<<<<..
.<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<....>>>.
..>>>...>>...<<<<<........>>>>>))<<<<<<<<<<<......
<<...<<<<....<<<<..<<...<<<<<.<<<<<<...(((....>>>>
>><<..<<<<<..<<<<.<<<<<<<<<.....>>>>>>>>>.....>>>>
..>>>>>...>>...<<.........>>....>>>>>....>>...>>>>
.........>>>>..>>..>>>>>>>>>>>>>>>.>>>.....<<.....
.>>>>>>>.<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<<.
..............<<<<<...............>>>>><<<<<<....>
>>>>>............<<<<<<<<<..<<<.........>>>..<<<<<
<<.....<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<
..<<<...<<...<<<<<<.<<....>>..>>>>>>...>>...>>>>>>
>>..>>>>><<<<<<.......>>>>>>........<...........<<
<<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>.....>>.
>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<
<<<<.(((.[[......<<<<.[[<<<<..<<<<<.<<<<<<<<<.<<<.
<<<<<<<.....>>>>>>>.>>>....<<.<<<<<<.....>>>>>>>>.
....>>>>>>>>>..>>>>>..<<<<.....>>>><<<<<<.........
..>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<
<.[.(((>>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<
<....>>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>..
...........>>>>..]]>>>>.......<<<<...<<<<<<...<<..
.....>>...>>>>>>....>>>.>.......<<<<<.<<<<<<..<<<<
<<<<<......>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..
>...>>>.>>....<<<<<<<....>>>.>>>>....]]..)))>>>>>>
>>....<<<<<<<...<<<<..<<<<<<<...............>>>>>>
>.<<<<<...<<<<...<<<<<<<<<<<.)))>>>>>>..>>>>>...>>
>>.>>>>>...<<<....<<<<<...........>>>>>....>>>.>>>
>.......>>>>>>>..<<<<.....>>>>....<<<<<<<<<.......
>>>>>....<<<<<<<<..<<.....<<<<....>>>>....>>...>>>
>>.>>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7p7u Structural basis for PoxtA-mediated resistance to phenicol and oxazolidinone antibiotics.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): R2 T4 K8 W16 P25 L26 G27 R28 A31 R38 K40 H48 T66 G67 K68 K69 K73 Y75 Y76 R77 H78 S79 Y81 G83 K86 R100 E103 S105 G108 M109 P111 N113 T114 L115 R117 K118 H133 A135 Q136 Q137 L146 I147
• Binding residue (residue number reindexed from 1): R2 T4 K8 W16 P25 L26 G27 R28 A31 R38 K40 H48 T66 G67 K68 K69 K73 Y75 Y76 R77 H78 S79 Y81 G83 K86 R100 E103 S105 G108 M109 P111 N113 T114 L115 R117 K118 H133 A135 Q136 Q137 L146 I147

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation
• GO:0017148 negative regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7p7u, PDBe:7p7u, PDBj:7p7u
• PDBsum: 7p7u
• PubMed: 35387982
• UniProt: A0A1B4XSB2