Structure of PDB 7oht Chain M Binding Site BS01

Receptor Information

>7oht Chain M (length=137) Species: 559292 (Saccharomyces cerevisiae S288C)

STDSIVKASNWRLVEVGRVVLIKKGQSAGKLAAIVEIIDQKKVLIDGPKA
GVPRQAINLGQVVLTPLTFALPRGARTATVSKKWAAAAVCEKWAASSWAK
KIAQRERRAALTDFERFQVMVLRKQKRYTVKKALAKA

Ligand information

>7oht Chain 1 (length=1485)

cuauuggaacaggacgucauagagggugagaaucccgugugggaccgaua
guaagaacugagagugaaaaaguacgugaaauuguugacaggccagcauc
aguuuugguggcaggauaaauccauaggaauguagcuugccugguaagua
uuauagccugugggaauacugccagcugggacugaggacugcgacguaag
ucaaggaugcuggcauugcgaguguuuggguguaaacccauacgcgaacg
uaggguccugggauaagagcauaguuccugccgaaguuucccucaggaua
gcagaagcucguaucaguuuuaugagguaaagcgaacuuuaaauauguaa
gaaguccuuguuugaacguggacauuugaaugaagagcuuuuagugggcc
auuuuugguaagcagaacuggcgaugcgggaugaaccgaacguagaguua
aggugccggaauacacgcucaucagacacacaaaagguguuaguucaucu
agacagccggacgguggccauggaagucggaauccgcuaaggagugugua
acaacucaccggccgaaugaacuagcccugaaaaggauggcgcucaagcg
uguuaccuauacucuaccgucaggguuaugcccugacgaguaggcaggcg
uggaggucagugagaagccugacaaggucgggucgaacggccucuaguga
aucagcgggggaagacccuguugagcuugacucuagguggggaguuuggg
ggcggcacaucuguuaaacgauaagcagauguccuaaggggggcucaugg
agaacagaaaucuccaguagaacaaaaggaaaagcccccuugauuuugau
uuucagugugaauacaaaccaugaaaguguggccuaucgauccuuuauag
aggugccagaaaaguuaccacagggauaacuggcuuguggcagucaagcg
uuagcgacauugcuuuugauucuucgaugucggcucuuccuaucauaccg
aagcagaauucgguaagcguuggauuguucacccacuaauagggagucug
gguuuagaccgucgugagacagguuaguuccuacugaugaauguuaccgc
aauaguaauugaacuuaguacgagaggaacaguucauucggauaauuggu
uuuugcggcugucugaucaggcaugccgcgaagcuaccauccgcuggauu
auggcugaacgccucuaagucagaauccaugcuagaacgcggugauuucu
ugcuccacacaauauagauggauacgaauaaggcguccuuguggcgucgc
ugaaccauagcaggcuagcaacggugcacuuggcggaaaggccuugggug
cuugcuggcgaauugcaaugucauuuugcguggggauaaaucauuuguau
acgacuuagauguacaacgggguauuguaagcaguagaguagccguuacg
aucugcugagauuaagccuuuguugucugauuugu

<..............((......<<<.....))>>>....>.........
.......<...>.............................<<<<<<<.<
<<<<<<<<<<<<<<.....<<<<<<<<<......<<<<<<...>>>>>>.
......>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>
>>.....>>>>>>>..<<<........<<<<....>>>>...........
.....<<<..>>>.....>>>.....<<<<<<....<.<<<....>>>.>
<<.<<.<......<<<.<<<<<<.......................>>>>
...<<<<..<<<..>>>..>>>>.....>>>>>.....>.>>.>>..<<<
<.<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<..
<<<<(((.....<<<<<<.....<<.........<<<.<<<<<<<<<<..
.....<<<<<.<<....<<.........>>....>>.........<<...
...>>...>>>>>...>>>>>>>>>>>>>.........))).>>...>>>
>>>.>>>>..>>>>>>><<<<<<<<<...>>>>>>>>>....>>>>>>..
.<<<<<<..<<.....<<<<<<.....>>>>>>...>>.>>>>>>.....
<<<<<..<<......<<<<<.<<.<<<..<<<<<......>>>>>.....
.<.<..<<.<<<<<..........>>>>>.>>.....<<<<<<<...<<<
.<.........>.>>>.................>>>>>>>...<<<..<<
<<.......>>>>..>>>.<<<....>>>...>.>........<.....>
............>>>...>>>>>>>..............<<<<<...<<.
...>>...>>>>>.................<<<...<<<<<..<.<<<<<
<<......>>>>>>>...>.<<<.........>>>.....>>>>>..>>>
......<<<<<<<....>>>.>>>>.....>>.>>>>>....<<<<<<<<
..<<<<.<.<<<<<<...............>>>>>>.><<<<<...<<<<
...<<<<<<<<<<<....>>>>>.>>>>>>..>>>>..>>>>>.<<<<..
.<<<<<...........>>>>>...>>>>.>>>>....>>>>>>>>....
..<<<<<<.<<<.<<....<<..........<<<<<<.....>>>>>>..
....>>.......<<<<<<...<<<<<.<..<<<......>>>..>.>>>
>>.>>>>>>.............>>.>>>.>>>>>>..<<<<<.<......
.......>.....<<<<<<<<<.<<<....<<<<.<..<<<<<.>>>>>.
.>>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7oht Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 4.7 Å
• Binding residue (original residue number in PDB): I6 W12 K42 N59 V64 P73 R74 G75 R77 T78 A79 T80 K83 K84 S98 W99 R106 R109 F118 M121 R124 K125 Q126 R128 Y129 K132 K133 K137
• Binding residue (residue number reindexed from 1): I5 W11 K41 N58 V63 P72 R73 G74 R76 T77 A78 T79 K82 K83 S97 W98 R105 R108 F117 M120 R123 K124 Q125 R127 Y128 K131 K132 K136

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0000470 maturation of LSU-rRNA
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0016236 macroautophagy
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0032991 protein-containing complex
• GO:0043232 intracellular non-membrane-bounded organelle
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7oht, PDBe:7oht, PDBj:7oht
• PDBsum: 7oht
• PubMed: 34813592
• UniProt: P36105|RL14A_YEAST Large ribosomal subunit protein eL14A (Gene Name=RPL14A)