Structure of PDB 6vwn Chain M Binding Site BS01

Receptor Information

>6vwn Chain M (length=114) Species: 562 (Escherichia coli)

KSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNGSEVLVAAST
VEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGRV
QALADAAREAGLQF

Ligand information

>6vwn Chain 3 (length=120)

ugccuggcggccguagcgcgguggucccaccugaccccaugccgaacuca
gaagugaaacgccguagcgccgaugguaguguggggucuccccaugcgag
aguagggaacugccaggcaa

<<<<<<<<<......<<<<<<<<....<<.<<<...............>>
>...>>...>>>>>>.>>.<<.......<<<<<<<<...>>>>>>>>...
....>>....>>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6vwn mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): R15 R25 H29 R30 T31 P32 R33 H34 Y36 Q38 I40 S45 V47 V54 E55 Y64 N67 K68 H100 G101 R102
• Binding residue (residue number reindexed from 1): R12 R22 H26 R27 T28 P29 R30 H31 Y33 Q35 I37 S42 V44 V51 E52 Y61 N64 K65 H97 G98 R99

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6vwn, PDBe:6vwn, PDBj:6vwn
• PDBsum: 6vwn
• PubMed: 32427100
• UniProt: P0C018|RL18_ECOLI Large ribosomal subunit protein uL18 (Gene Name=rplR)