Structure of PDB 6gfw Chain M Binding Site BS01

Receptor Information

>6gfw Chain M (length=366) Species: 573 (Klebsiella pneumoniae)

YQDDELPVYQGETTQTLQDYLMWQVELTPFTDTDRAIATSIVDAVDDTGY
LTIQIEDIVDSIGDDEIGLEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQL
SQFAKETPWLEEARLIISDHLDLLANHDFRTLMRVTRLKEEVLKEAVNLI
QSLDAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAASIPRLKINQQYAA
MGNSARNDADGQFIRSNLQEARWLIKSLESANDTLLRVSRCIVEQQQAFF
EQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIAAAAAAAAA
AAAAAAGASSTAIRALVKKLIAAENPAKPLSDSKLTSMLSEQGIMVARRT
VAKYRESLSIPPSNQR

Ligand information

>6gfw Chain F (length=50)

tgcgcaacagcatgcgcgcccagggctgatcgtgcaaaagtcgtgccagc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6gfw Structures of Bacterial RNA Polymerase Complexes Reveal the Mechanism of DNA Loading and Transcription Initiation.
• Resolution: 3.7 Å
• Binding residue (original residue number in PDB): Y104 Q105 L109 V111 A336 T339 L340 H377 T380 T457
• Binding residue (residue number reindexed from 1): Y1 Q2 L6 V8 A231 T234 L235 H272 T275 T350

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Gene Ontology

Molecular Function

• GO:0001216 DNA-binding transcription activator activity
• GO:0003677 DNA binding
• GO:0016779 nucleotidyltransferase activity
• GO:0016987 sigma factor activity

Biological Process

• GO:0006352 DNA-templated transcription initiation
• GO:0006355 regulation of DNA-templated transcription
• GO:0009399 nitrogen fixation
• GO:0045893 positive regulation of DNA-templated transcription
• GO:2000142 regulation of DNA-templated transcription initiation

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex

External links

• PDB: RCSB:6gfw, PDBe:6gfw, PDBj:6gfw
• PDBsum: 6gfw
• PubMed: 29932903
• UniProt: P06223|RP54_KLEOX RNA polymerase sigma-54 factor (Gene Name=rpoN)