Structure of PDB 6dzi Chain M Binding Site BS01

Receptor Information

>6dzi Chain M (length=145) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

VIKLHDLKPAPGEKKAKTRVGRGEGSKGKTAGRGTKGTKARKNVPVMFEG
GQMPIHMRLPKLKGFKNRFRTEYQVVNVGDINKAFPQGGTVGVDELVAKG
LVRKNSLVKVLGDGKLTVKVDVTANKFSGSAREAITAAGGSATEL

Ligand information

>6dzi Chain A (length=3119)

aaguguuuaagggcgcaugguggaugccuuggcacugggagccgaugaag
gacguaggaggcugcgauaagccucggggagcugucaaccgagcguugau
ccgaggauguccgaauggggaaacccggcacgagugaugucgugucacca
ggcgcugaauauauaggcgucuggggggaacgcggggaagugaaacaucu
caguacccguaggaagagaaaacaaaaugugauuccgugaguaguggcga
gcgaaagcggaggauggcuaaaccguaugcaugugauaccggguaggggu
ugugugugcgggguugugggaccuaucuuuccggcucuaccuggcuggag
ggcagugagaaaauguugugguuagcggaaauggcuugggauggccugcc
guagacggugagagcccgguacgugaaaacccgacgucugucuugauggu
guucccgaguagcagcgggcccguggaaucugcugugaaucugccgggac
cacccgguaagccugaauacuucccagugaccgauagcggauuaguaccg
ugagggaauggugaaaaguaccccgggaggggagugaaagaguaccugaa
accgugcgcuuacaauccgucagagcccucgacgugucguggggugaugg
cgugccuuuugaagaaugagccugcgagucagggacaugucgcgagguua
acccgggugggguagccgcagcgaaagcgagucugaauagggcguaucca
cacaagagugugugguguagugguguguucuggacccgaagcggagugau
cuacccauggccagggugaagcgcggguaagaccgcguggaggcccgaac
ccacuuagguugaagacugaggggaugagcuguggguaggggugaaaggc
caaucaaacuccgugauagcugguucuccccgaaaugcauuuaggugcag
cgucgcauguuucuugccggagguagagcuacuggauggccgaugggccc
cacaggguuacugacgucagccaaacuccgaaugccgguaaguccaagag
ugcggcagugagacggcgggggauaagcuccgugcgucgagagggaaaca
gcccagaucgccggcuaaggccccuaagcgugugcuaaguggaaaaggau
gugcagucgcgaagacaaccaggagguuggcuuagaagcagccacccuug
aaagagugcguaauagcucacuggucaagugauugugcgccgauaaugua
gcggggcucaagcacaccgccgaagccgcggcagccaacguguuggcugg
guaggggagcguccugcauccggugaagccgccgagugaucgaguggugg
agggugugggagugagaaugcaggcaugaguagcgauuaggcaagugaga
accuugcccgccgaaagaccaaggguuccugggccaggccaguccgccca
gggugagucgggaccuaaggcgaggccgacaggcguagucgauggacaac
ggguugauauucccguacccguguaugugcguccaugaugaaucagcggu
acuaaccauccaaaaccaccgugaccgcaccuuucgggguguggcguugg
uggggcugcaugggaccuucguugguaguagucaagcgauggggugacgc
aggaagguagccguaccggucagugguaauaccgggguaagccuguaggg
agucagauagguaaauccgucuggcauauauccugagaggugaugcauag
ccgagugaggcgaauucggugauccuaugcugccgagaaaagccucuagc
gaggacauacacggcccguaccccaaaccaacacagguggucagguagag
aauacuaaggcguacgagugaacuaugguuaaggaacucggcaaaaugcc
cccguaacuucgggagaagggggacccacauggcguguaagccuuuacgg
cccaagcgugaguggguggcacaaaccagugagaagcgacuguuuacuaa
aaacacagguccgugcgaagucgcaagacgauguauacggacugacgccu
gcccggugcuggaagguuaagaggacccguuaacucccuuugggggugaa
gcggagaauuuaagccccaguaaacggcggugguaacuauaaccauccua
agguagcgaaauuccuugucggguaaguuccgaccugcacgaauggcgua
acgacuucucaacugucucaaccauagacucggcgaaauugcacuacgag
uaaagaugcucguuacgcgcggcaggacgaaaagaccccgggaccuucac
uacaacuugguauuggugcucgauacgguuuguguaggauaggugggaga
cugugaagcucacacgccaguguggguggagucguuguugaaauaccacu
cugaucguauugggccucuaaccucggaccguauauccgguucagggaca
gugccugguggguaguuuaacuggggcgguugccuccuaaaauguaacgg
aggcgcccaaagguucccucaaccuggacggcaaucagguguugagugua
agugcacaagggagcuugacugcgagacggacaugucgagcagggacgaa
agucgggacuagugauccggcaccucugaguggaaggggugucgcucaac
ggauaaaagguaccccggggauaacaggcugaucuuccccaagaguccau
aucgacgggaugguuuggcaccucgaugucggcucgucgcauccuggggc
uggagcaggucccaaggguugggcuguucgcccauuaaagcggcacgcga
gcuggguuuagaacgucgugagacaguucggucucuauccgccgcgcgcg
ucagaagcuugaggaaaccugucccuaguacgagaggaccgggacggacg
aaccucugguauaccaguugucccaccaggggcacggcuggauagccacg
uucggacaggauaaccgcugaaagcaucuaagcgggaaaccucuuccaag
accaggcuucucacccucuaggagggauaaggccccccgcagaccacggg
auugauagaccagaccuggaagccuaguaauaggugcagggaacuggcac
uaaccggccgaaaacuuac

<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<<......<
<<<<<.((..((.>>>.....<<<<<<<..<<<))...)).>>>.....>
>>>>>>...>>>....<<<....>>>.<<<<<<......>>>>>>..<<<
<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<......>.
.....>>>>>.>.........<<.....>>..<<<<<<.....<<.....
>>....>>>>>>...<<<....<<<<<<<<<<.........<<<<.....
.>>>>>>>>>>......<<<<..[.[...<<<<<<..>>>>..>>>>>><
<<<<<........<<<<<.<<<<.<<<.....<<<<......>>>>.((.
.....<<<.....))>>>...>>>...>>>>>>>>>.>>>>>>..]..].
..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..(
(((>>>>>..>>>......>>.>>>>>>>.>>...............<<.
...>>....>>.........<<<<....>>>>..................
>>>>>>>>>>>.......<<<<..<<<<<<<<<..>>>>.>>>>>..>>>
>...<.<........>.>.<<<<<<....<<<.<<<<<<<<<<..<<<..
.<<<....>>>...>>>...<<....>>..<<<<.....>>>>....<<<
<<<<....>>>>>>>....>>>>>>>>>>>>>..<<.<..<<<<<<<...
..<<<<<<<<<..<<<....<<<<<<......>>>>>>.....>>>...<
<<.<<<<<........>>>>>>>>....>>>>>>>>>...<<<.....>>
>......>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>><
<<<<<<<<....<<<<<<<<.....<<<....<<<.<<<<.<....<<<<
....>>>>......>>>>>.>>>..>>.>.....>>>>>>>>.......>
>>>>>>.>>..<<<<<<<<<<......>>>>>>.>>>>....<<<.....
.>>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..
.<<<<<<<<........<<<<<.<<...<<<<.......>>>>...>>..
.......<<......>>...>>>>>....>>>>>>>>..>>.........
..)))......>>>>>>.>>>...>>>>>>><<<<<<<<..>>>>>>>>.
...>>>>>><.<<<<<<<<<<......<<<<<<<<....>>>.>>>>>..
.>>>>>>>>>>..>.....>>>>>><......<<.....<<<<<<.....
..>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>
>>....((<<<<.<<.<.<<<...<<<....>>>...>>>..>>>...<<
<<<........>>>>>))<<<<<<<<<<.......<<<<...<<<<<<..
.....<<<<.<....<<<<<....<<<<<<<<....>>>>>>>>....>>
>>>....>.>>>>......>>>>>>.....>>>>.<<...<<<<<...<<
<<..<<<..<<<<..<<<<<....[[[...>>>>>.<<..<<<<...<<<
<<<<<<<<.<<.....>>>>>>>>>.....>>>>...>>>>...>>...<
<<......>>>.....>>>>...>>>...>>>>........>>>>.>.>>
....>>>>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<<...
..>>>>..>>>>..<<<<<...<<<<<<<<.<<.........<.....<<
<<<.....<..>......>>>>><<<<<<...<<<<....<<<.....>>
>....>>>>..>>>>>>............<<<<<<<<<..<<<.......
..>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.
<<<..<<<<<<<..<<<...<<....<<<<<.<<<<<<...>>>>>>..>
>>>>....>>...>>>>>>>>..>>>>><<<<<<.......>>>>>>...
.....<...........<<<<<.......>>>>>.....>....>>>>>.
.>>.>>>>>>>..>..>>.>>>>>>>>>>>>>>>.....>.((..<<<<<
......))>>>>>..<<<<<<<<.<<<.<<......<<<<<.<<<<..<<
<<<.<<<.<<<<<.<<.<<<<<<<<<<<..............<<<<....
.......<<<<<<<<....>>>>>>>>..................>>>.>
....>>>>>>>>>>>.>>...<<<.<<<<<<......>>>>>>>>>....
>>>>>.>>>..>>>>>..<<<<.....>>>><<<<<<...........>>
>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.
(((>>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<<...
.>>>.>>)))]......<<<<<<<.<<..))))..>>>>>>>>>......
.......>>>>...>>>>>.......<<<<...<.<<<<...<<......
.>>...>>>>.>....>>>.>.......<<<<<.<<<<<<..<<<<<<<<
<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>...
>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>>>>>>>>>..
..<<<<<<<...<<<<..<<<<<<<...............>>>>>>>.<<
<<<...<<<<...<<<<<<<<<<.]]]..>>>>>..>>>>>...>>>>.>
>>>>...<<<....<<<<<...........>>>>>....>>>.>>>>...
....>>>>>>>..<<<<<...>>>>>....<<<<.<<<<.......>>>>
.....<<<.<<<<.<<<....<<<<<....>>>>>...>>>..>>>>..>
>>...>>>>....>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6dzi Zinc depletion induces ribosome hibernation in mycobacteria.
• Resolution: 3.46 Å
• Binding residue (original residue number in PDB): H7 K10 E15 K16 T20 R21 V22 G23 R24 K29 K31 T32 A33 G34 R35 T37 K38 G39 T40 K41 R43 K44 F50 G52 G53 Q54 M55 R60 K63 K65 G66 F67 K68 N69 R70 F71 R72 V77 N79 R105 K106 K111 L113 S132
• Binding residue (residue number reindexed from 1): H5 K8 E13 K14 T18 R19 V20 G21 R22 K27 K29 T30 A31 G32 R33 T35 K36 G37 T38 K39 R41 K42 F48 G50 G51 Q52 M53 R58 K61 K63 G64 F65 K66 N67 R68 F69 R70 V75 N77 R103 K104 K109 L111 S130

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6dzi, PDBe:6dzi, PDBj:6dzi
• PDBsum: 6dzi
• PubMed: 30038002
• UniProt: I7G436