Structure of PDB 8oj0 Chain Li Binding Site BS01

Receptor Information

>8oj0 Chain Li (length=102) Species: 9606 (Homo sapiens)

ALRYPMAVGLNKGHKVTKNVSKPRHSRRRGRLTKHTKFVRDMIREVCGFA
PYERRAMELLKVSKDKRALKFIKKRVGTHIRAKRKREELSNVLAAMRKAA
AK

Ligand information

>8oj0 Chain 5 (length=3474)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggacau
guggcguacggaagacccgcuccccggcggggggcccaaguccuucugau
cgaggcccagcccguggacggugugaggccgguagcggcccccggcgcgc
cgggcccgggucuucccggagucggguugcuugggaaugcagcccaaagc
gggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauaguca
acaaguaccguaagggaaaguugaaaagaacuuugaagagagaguucaag
agggcgugaaaccguuaagagguaaacgggugggguccgcgcaguccgcc
cggaggauucaacccggcggcggguccggccgugccggcgaccggccgcc
gccgggcgcauuuccaccgcggcggugcgccgcgaccggcuccgggacgg
cugggaaggcccggcggggaagguggcucgggggcccccgaguguuacag
cccccccggcagcagcacucgccgaaucccggggccgagggagcgagacc
cgucgccgcgcucuccccccggggggccgggccaccccucccacggcgcg
accgcucggggcggacuguccccagugcgccccgggcgggucgcgccguc
gggcccggggggccacgcgcgcgucccccgggggacggcggagcgagcgc
acggggucggcggcgacgucggcuacccacccgacccgucuugaaacacg
gaccaaggagucuaacacgugcgcgagucgggggcucgcacgaaagccgc
cguggcgcaaugaaggugaaggccggcgccggccgaggugggaucccgag
gccucuccaguccgccgagggcgcaccaccggcccgucucgcgggaggug
gagcacgagcgcacguguuaggacccgaaagauggugaacuaugccuggg
cagggcgaagccagaggaaacucugguggagguccguagcgguccugacg
ugcaaaucggucguccgaccuggguauaggggcgaaagacuaaucgaacc
aucuaguagcugguucccuccgaaguuucccucaggauagcuggcgcucu
cgcagacccgacgcaguuuuauccgguaaagcgaaugauuagaggucuug
gggccgaaacgaucucaaccuauucucaaacuuuaaauggguaagaagcc
cggcucgcuggcguggagccgggcguggaaugcgagugccuagugggcca
cuuuugguaagcagaacuggcgcugcgggaugaaccgaacgccggguuaa
ggcgcccgaugccgacgcucaucagaccccagaaaagguguugguugaua
uagacagcgccgaaucaacuagcccugaaaauggauggcgcuggagcguc
gggcccauacccggccgucgccggcagucgagaguggacgggagccccgc
ggacgcuacgccgcgacgaguaggagggccgcugcggugagccuugaagc
cuagggcgcgggcccggguggagccgccgcaggugcagaucuugguggua
guagcaaauauucaaacgagaacuuugaaggccgaaguggagaaggguuc
caugugaacagcaguugaacaugggucagucgguccugagagaugggcga
gcgccguuccgaagggacgggcgcccucggccgaucgaaagggagucggg
uucagauccccgaauccggagcggagaugggcgccgcgaggcguccagug
cgguaacgcgaccgaucccggagaagccggcgggagccccggggagaguu
cucuuuucuuugugaagggcagggcgcccuggaauggguucgccccgaga
gaggggcccgugccuuggaaagcgucgcgguuccggcggcguccggugag
cucucgcuggcccuugaaaauccgggggagaggguguaaaucucgcgccg
ggccguacccauauccgcagcaggucuccaaggugaacagccucuggcau
guuggaacaauguagguaagggaagucggcaagccggauccguaacuucg
ggauaaggauuggcucuaagggcugguagcagccgacuuagaacuggugc
ggaccaggggaauccgacuguuuaauuaaaacaaagcaucgcgaaggccc
gcggcggguguugacgcgaugugauuucugcccagugcucugaaugucaa
agugaagaaauucaaugaagcgcggguaaacggcgggaguacucucuuaa
gguagccaaaugccucgucaucuaauuagugacgcgcaugaauggaugaa
cgagauucccacugucccuaccuacuauccagcgaaaccacagccaaggg
aacgggcuuggcggaaucagcggggaaagaagacccuguugagcuugacu
cuagucuggcacggugaagagacaugagagguguagaauaagugggaggc
ccgccggugaaauaccacuacucugauguuuuuucacugacccggugagg
cgggggggcgaucucgcuucuggcgccaagcgcccggccgcggccgggcg
cgacccgcuccggggacagugccagguggggaguuugacuggggcgguac
accugucaaacgguaacgcagguguccuaaggcgagcucagggaggacag
aaaccucccguggagcagaagggcaaaagcucgcuugaucuugauuuuca
guacgaauacagaccgugaaagcggggccucacgauccuucugaccuuuu
ggguuuuaagcaggaggugucagaaaaguuaccacagggauaacuggcuu
guggcggccaagcguucauagcgacgucgcuuuuugauccuucgaugucg
gcucuuccuaucauugugaagcagaauucaccaagcguuggauuguucac
ccacuaauagggaacgugagcuggguuuagaccgucgugagacagguuag
uuuuacccuacugaugauguguuguugccaugguaauccugcucaguacg
agaggaaccgcagguucagacauuugguguaugugcuuggcugaggagcc
aauggggcgaagcuaccaucugugggauuaugacugaacgccucuaaguc
agaaucccgcccaggcggaacgauacggcagcgccgcggagccucgguug
gccucggauagccgguccccgggguccggugcggagugcccuucguccug
ggaaacggggcgcggcuggaaaggcggccgcccccucgcccgucacgcac
cgcacguucguggggaaccuggcgcuaaaccauucguagacgaccugcuu
cugggucgggguuucguacguagcagagcagcgcugcgaucuauugaaag
ucagcccucgacacaaggguuugu

.........................<<<<<<.....<......>......
>>>>>.>.........<<....>>..<<<<<<.....<<.....>>....
>>>>>>..<<<..<<<<<...<<<<<<<<<<.....>>>>>>.>>>>...
..>>>>>..<<<<<<<<<<...<<<<<<.<<<<<<<<....<<<<.....
.>>>>.....((......<<<.....))>>>...>.>>>>>>>.....>>
>>>>..>>>>>>>>>>.......<<<<<<<........>>>>>>>.....
<<<<<..((((((>>>>>..>>>...........................
.......<<....>>...............<<<<....>>>>........
..........................<<<<<<<<..<<<.<<...<<<<<
<<<.<<<.....<<<<<<<<<<<..<<<<<<<...>>>>...>>>>>>>>
>>>>>>......>>>.>>>.>>>>>...<<<<<<<<<<<<<<<<<<<<<<
<...<<..<<<..<.<<<<......<<<<<<<<..>>>>>>>>.......
>>>>.>.>>>........>>>>>>..>>>>>>>>>>>.............
.......<<<<<<.<<<<<..>>>>>.<<<<<<.<<<<.<<<<<<<<<<<
<<<<<<<<<<<<<.<<<<....>>>>.>>>>>>>>>>>.>>>>>>>>>>.
>>>...>>>>>>>>.>>..<<<<<<<<<..>>>>>>>.>>....>>>>>>
.....>>>.>>>>>...>>>>>...>>>>>>>>..<<<<........>>>
><<<<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>..
...>>>...<<....>>...<<<<<<..>>>>>>..<<<<<...<<<..<
<<..((((.((((>>>..>>>....>>>>>.<<<<<<<<<....>>>>>>
>.>>....>>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<
..<<<....<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<..
......>>>>.>>>....>>>>>>>>>...<<.......>>......>>>
>>>...>..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<.
............<<<.<<<<<<<<<...<<<....<<<..<.<<<..<<<
<<<.<<...>>.>>>>>>>>>.>..>>>..>.>>...>>>>>>>...<<<
<<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>>.>>>..<<<<
.<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<...
<<<(((.......<<<<<.....<<..........<<<.<<<<<<<<<<.
......<..>...>>>>>>>>>>>>>..........))).>>...>>>>>
..>>>...>>>>>>><<<<<<.<<<..........)))).))))......
.........>>>>>>>>>....>>>>>>...<<<<<<<<.<<......<<
<<.<<<<....>>>>>>>>...>>>>>>>>>>........>>>>>><<..
...<<.....<<<<<<.......>>>>>>.<<<.....<<<<<.<<...<
<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<....<<<..
..>>>..........<<.<<<..>>>>>......>>....>>>..<<<<<
........>>>>>))<<<<.<<<<<..<<<<<<<<....>>>>>>>>..<
<<....>>>...<..<<<<<<<...<<<<<<<<<<<<.<<<<<<<<<<..
[[[..>>>>>>....<<<<<.<<<<.<<<<.....<<<.....>>>....
.>>>>>>>>..>>>>>.....<<<<<<<.......>>>>>>>>>>>...>
>>>>>>>>>>>.........>>>>>>>..>..........>>.>>>.>>>
>.>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>..
..<<<<.....<<<<<<<.<<...............<<<<<....<<..>
>.....>>>>><<.<<<<<<<<<<<....>>>>>..>>>>>>.>>.....
.......<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<
.....>>>>......>>>>>>>...<<..<<<..<<<<<<....<<<...
..<<<<....>>>>.....>>>.>>>>..>>>>><<<<<..>>>>>....
....<...........<<<<<.......>>>>>.....>.....>>....
>>.>>>>>>>.....>>.>>>>>>>..>>>>>>....>>....<<<<<..
......>>>>>....<<<<<..<<<.<<......<<<<.<<<<<<..<<<
<<.<<<<<<<<<.<<<<<<<<<<<<.<<<<...........<..<<....
..............>>..>.>>>>.>>>>>>>>>>>>...<<<<<.....
<<<<..<<.<..>>>..............<<<<<<<<<<..>>>>>>>>>
>..>>>>..>>>>>....>>>>>>>>>..>>>>>...<<<.....>>>..
<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<....
...>>>>>>.....<<.[.(((>>....>>>>>>>...<<<..<<<<...
....>>>>..>>>.<<<....>>>.>>)))].....<<<<<<<..<<<..
)))))).>>>>>>>>>>.........>>>>..>>>>>>.......<<.<.
<.<<<<<<...<<.......>>...>>>>>>...>>.>.>.......<<<
<<.<<<<<<..<.<.<<<<<......>>>>>...>.>.<<<.........
>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>..
..>>..>>>.>>>>>.....<<<<<<.<..<<<..<.<<<<<........
.........>>>>>.><<<<....<<<<....<<.<<<<<<.]]]..>>>
>..>>.>>...>>>>...>>>><<<<....<<<<<...........>>>>
>....>>>>.>>>...>.>>>>>>.....<<<<<<<.<<..<<<<....<
<<........<<<<<.<<<<>>>>.>>>>>.......>>>...<<<..<<
<<....<<<<.<<<<<<<......>>>>>>>>>>>...>>>>..>>>...
...........>>>>..>>>>>>>>><<<<<.<.............>...
.<<.<<<<<<<.<<.....<<<<.<..<<<<..>>>>..>>>>>....>>
....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8oj0 UFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): H15 V17 R25 H26 S27 R28 R29 R30 G31 R32 L33 T34 H36 R41 Y53 E59 R68 K75 R76 V77 G78 T79 H80 R82 K84 R85
• Binding residue (residue number reindexed from 1): H14 V16 R24 H25 S26 R27 R28 R29 G30 R31 L32 T33 H35 R40 Y52 E58 R67 K74 R75 V76 G77 T78 H79 R81 K83 R84

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0016020 membrane
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0045202 synapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8oj0, PDBe:8oj0, PDBj:8oj0
• PDBsum: 8oj0
• PubMed: 38383785
• UniProt: Q9Y3U8|RL36_HUMAN Large ribosomal subunit protein eL36 (Gene Name=RPL36)