Structure of PDB 8bsj Chain Lg Binding Site BS01

Receptor Information

>8bsj Chain Lg (length=98) Species: 5741 (Giardia intestinalis)

EEIKGKTFLPAIFVGYQRSRAKQRENVALVKVSGIYTRAETDKVLGRLIA
IIHSKSGKPIFGKICRPHGNSGMVRARFYTNLPGQLMGHKVRVFYDNI

Ligand information

>8bsj Chain Lt (length=2593)

ggcgggccugaggcggcgggggcgacgggcggaacuuaagcauaucagua
cgccccggaggagaaaccaaccgggauuccccgcuagcggcgagcgacgc
gggaggagcccgccccgaaggcgcgcuguggggccgcgagggggcccgag
ggccccgcccgagagggugcaagccccguacggcgaguagcgcugcuuga
gcgugcagcgcgaagggaggcgcggcccuuccaaggcuaaauacgccccg
ggaccgauagcggaccaaguagcgcgagcgaacggugaaaaggacgcccu
gcggccgcucaaaagaccugaacccggccggccgccggcccgccggcccc
gucucgaaacacggaccgaggagccacgcgccgcggcgagcccgagggag
cccccgcggcggagcgagcgcgagacgccccgggcccgccaugccccugc
gggcgugcgcgggccgagccgcggcgcgugggcccgaaaggcggugaucu
augcccggcgagggcgaggccgggcgaaagccugguggaggcccgccgcg
gugcugacgcgcagaucgcucgucggagccgggcaugggggcgaaagacu
caucgaaccgccugguagcugguugccuccgaaaugucucccaggacagc
cgccgccccgcaguugcggcccguagagcgcuggccggcgggagcggggg
gccugccccucgcccgccccccaaacuccgaagggccgcgccgccccgcc
gcuggccugggcggggcgggcgaaugcgggcggcgcgugggccccuccug
guaagcaggacgggcgaggcgggacgauccggacgccgggccagggugcg
ccgccggggcccgcggaacggcgucggccggucccgacagccgaaucggc
cggcccggaaaauggagcgcgccggagccccggacccgcgcccggccgcc
gcgcgcggcggguaggaggccgcagaggccccgggggcgaaggcggcgcg
caggccccgccggaccggccucuggugcagaucucggcagcaguagccgc
uacuccgcgccccggaggacugagggggagacggguuccgcggcgccugc
aucuggccgcgggugacucgggccuaagcggcgggugaagaccgggaagg
ggcgcccgcccgucgaacggggagccggcggagacuccggcaggcgcggc
ccccgcggagacgcccgccccccggcgacgcgcacggggaccgcggcggg
cggcgccccggcccgcgaacgccccgcagcccccggacgccuugcgcgga
gaggggggcccgggggcggaccgcgcguccccggccgccccugaaaagcc
ggggggcgccggccgcgcgccguaccgaccgcagcaggacuccgggguca
gcagccucuagcgcgggagcgaacgcggcucagggaagucggcaagccgg
cuccguaaccucgggaaaaggaguggcucugacggcgccgggucagaacu
ggaacggacgcggggaucccgacuguuuacuagaaacacagcgucgcgag
ggccgcacccggcgcuggcgcgacgugauuucugcccagugccacgaccg
ucaccgugaagcgauccgccgaagcccugguaaacggcgggaguaacuau
gacucucuuaagguagccaaaugccucgucgggcaauuuccgacgugcau
gaauggaccaacgaggaucccacugucccgagccgcgccuccgcgagccu
ccagccucgggaacgggcgagggccggccagcggggcaagaagacccuuu
ugagcuugacuccagcccgggccuguggggcggggcggccggcgcagcgc
acaggggaggccgcgccccugagacacccugacggccgccgccgccccgc
ucacccggucgcgcggggacccgcccgggcggggaguucggcuggggcgg
cgcgccugcuacaccggaccgcaggcgucccacggcgggcucagcgagga
cggagaccucccgcggagcagaagggcacaagcccgcccgacccgcgccc
cccgugccggcgcgggccgcgaaagcggggccuaccgauccuucgccgcc
ccggccgcgggcgcggagguggcagaaaaguuaccacagggauaacuggc
uuguggccgccgagcgcccgcagcgacgcggcuuuuugauccuucgaugu
cggcucuuccuaccguccgcgcgcaccggcgcggaagcgucggauuguuc
acccguucaagggaucgugagcuggguuuagaccgucgugagacagguua
guuuuacccuacuggccccggggccagagcacggcgggccaguacgagag
gaacgcccgccgcgggccgccagccccgcgguugcccggccgggcagcgc
cgcgccgccgcgcccgggggcccugcgcugaccgccucuaagcgcgcacc
ccgccucgcgccccgcccggccgcgcgccccagccccgugccccgucgcc
gagcggcccccgcccggggagaccacccggcgcggcgcuccuguacggcg
cagagcccugcgaucgccugagggacgcgccugcagagcgcgg

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.....................<<....>>................<<<..
..>>>............................<<<<<<..>>>>>>.<<
<<........>>>>.<<<<<....<<<<<<<<<<<<<..<<<...<<<..
..>>>..>>>...<<....>>............<<<<<<<<<<<<<....
>>>>>>>.>>>>>>...>>>>>>>>>>>>>..<<.<..<<<<<<<.....
<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<
..<<.<<........>>>>.>>>....>>>>>>>>>...<<.......>>
......>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>><<
<<<<<<<<.<<<.<<<<<<<<<...<<<...<<<..<<<<<..<<<<<..
........>>>>>>>>>>..>>>..>>.>...>>>>>>>.<<<<<<<<<<
.........>>>>>>>>>>...>>>>>>>>>>>>>.>>..<<<<.<<<<<
.....>>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(((
...<<<<<<<........<<<.<<<<<<<<<..............>>>>>
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<....>>>>>....>>>>>><<<<<<<<<<...<<......<<<<<.<<.
...>>.>>>>>...>>>>>>>>>>.>>.....>>>>>.<<.....<<...
..<<<<<.....>>>>>..<<<....<<<<..<<...<<<<<<...<...
....>.>>>>>>....((<<<.<<...<<<...<<<....>>>......<
<<..>>>...>>.>....>>...<<<<<........>>>>>))<<<<<<<
<...<<<....>>>..<<<<<<<<<<...<<<..<<<<<<<<<<<<<<<<
<...[[[...>>>>>....<<<<<<...<<<<<<....<<.....>>...
...>>>>>>...>>>>>>..>>>>>.>>>>>>>..>>>.........>>>
>>>>>>>...>>>>>>>>.>>>..>>..<<......>>.>>>>.<<<<..
...>>>>.>>>....<<<<....<<<<<<<<.<<.........<.....<
<<<<...............>>>>><<.<<<<<<<<..>>>..>>>>>.>>
............<<<<<<<<<..<<<.........>>>..<<<<<<<...
.<<<......>>>......>>>>>>>...<<..<<<..<<<<<<.<...<
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.>>>>>.........<...........<<<<<<.....>>>>>>.....>
.....>>....>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>....>>
.(..<<<<<......).>>>>>...<<<<<<..<<<.<<......<<<..
<<<<<<..<<<<<.<<<<<<<<..<<<<<<<<<.<<<...<.........
.<<.<......................>.>>.>...>>>..>>>>>>>>>
...<<<<......>>>>.....>>>>>>>>..>>>>>...<<<.....>>
>..<<<<<<...........>>>>>>..<<...<<<<<<<...<.<<<<.
......>>>>.>.....<<...(((>>....>>>>>>>...<<<<<<<<.
........>>>>>>>>.<<<....>>>.>>)))......<<<<<<<.<<<
<.))))))>>>>>>>>>>>.........>>>>..>>.>>>.......<<.
<.<.<<<<<<...<<.......>>...>>>>>>...>>.>.>.......<
<<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<<.<.....
>.>>>....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>.
...>>..>>>.>>>>>>..<<<<<..<<<..<<<<<<<<...........
....>>>>>>>><<<<<...<.<<...<<<<<<<<<<]]].>>>>>..>>
>>>...>>.>.>>>>>.<<<...<<<<<<...........>>>>>>...>
>>..>>>..>>>>>....<<<<<<..........................
..>>>>>>...<<.<<<<.......>>>>..<<<<<<<<<<<....<<<<
....<<...>>...>>>>...>>>>.>>>>>.>>.....>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8bsj Insights into translocation mechanism and ribosome evolution from cryo-EM structures of translocation intermediates of Giardia intestinalis.
• Resolution: 6.49 Å
• Binding residue (original residue number in PDB): K5 K7 T8 F9 Q18 R19 S20 R21 Q24 E26 R39 H54 R67 H69 N71 S72 M74 R76 N82 P84 G85 Q86 M88 K91 R93
• Binding residue (residue number reindexed from 1): K4 K6 T7 F8 Q17 R18 S19 R20 Q23 E25 R38 H53 R66 H68 N70 S71 M73 R75 N81 P83 G84 Q85 M87 K90 R92

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8bsj, PDBe:8bsj, PDBj:8bsj
• PDBsum: 8bsj
• PubMed: 36912103
• UniProt: A8BKK5