Structure of PDB 8oo0 Chain Le Binding Site BS01

Receptor Information

>8oo0 Chain Le (length=124) Species: 209285 (Thermochaetoides thermophila)

VAAKKHVPIVKKRTKRFMRHQSDRFKCLDSAWRKPKGIDNRVRRRFKGNL
AMPSIGYGSNKKTKHMMPSGHKAFLVHNVKDVELLLMHNRTYAAEIAHNV
SSRKRIDIITRAKQLGVKVTNAKA

Ligand information

>8oo0 Chain 1 (length=3191)

guugaccucggaucagguaggaggacccgcugaacuuaagcauaucaaua
agcggaggaaaagaaaccaacagggauugcccuaguaacggcgagugaag
cggcaacagcucaaauuugaaagcuggcuucggcccgcguuguaauuugg
agaggaugcuuugggcgaggcuccuucugaguucccuggaacgggacgcc
acagagggugagagccccguauaguuggaagccaagccuguguaaagcuc
cuucgacgagucgaguaguuugggaaugcugcucaaaaugggagguaaau
uucuucuaaagcuaaauaccggccagagaccgauagcgcacaaguagagu
gaucgaaagaugaaaagcacuuugaaaagaggguuaaauagcacgugaaa
uuguugaaagggaagcgcuugugaccagacuugcgcccggcggaucaucc
gguguucucaccggugcacuccgccgggcucaggccagcaucgguucugg
cggggggauaaaggcccagggaauguggcuccuccgggaguguuauagcc
cuggguguaauacccucgccgggaccgaggaccgcgcucugcaaggaugc
uggcguaauggucaccagcgacccgucuugaaacacggaccaaggaguca
agguuuugcgcgaguguuuggguguaaaacccgcacgcguaaugaaagug
aacguaggugagagcuucggcgcaucaucgaccgauccugauguauucgg
auggauuugaguaggagcguuaagccuuggacccgaaagauggugaacua
ugcuuggauagggugaagccagaggaaacucugguggaggcucgcagcgg
uucugacgugcaaaucgaucgucaaaucugagcaugggggcgaaagacua
aucgaaccaucuaguagcugguuaccgccgaaguuucccucaggauagca
gugucgaccuucaguuuuaugagguaaagcgaaugauuagggacucgggg
gcgauuuuuagccuucauccauucucaaacuuuaaauauguaagaagccc
uuguuacuuaacugaacgugggcauucgaauguaucgacacuagugggcc
auuuuugguaagcagaacuggcgaugcgggaugaaccgaacgcgggguua
aggugccggaguggacgcucaucagacaccacaaaaggcguuaguacauc
uugacagcaggacgguggccauggaagucggaauccgcuaaggacugugu
aacaacucaccugccgaauguacuagcccugaaaauggauggcgcucaag
cgucccacccauaccccgcccucaggguagaaacgaugcccugaggagua
ggcggccguggaggucagugacgaagccuagggcgugagcccgggucgaa
cggccucuagugcagaucuuggugguaguagcaaauacuucaaugagaac
uugaaggaccgaaguggggaaagguuccaugugaacagcgguuggacaug
gguuagucgauccuaagccauagggaaguuccguuucaaaggggcacucg
ugccccguguggcgaaagggaagccgguuaauauuccggcaccuggaugu
ggguuuugcgcggcaacgcaacugaacgcggagacgacggcgggggcccc
gggcagaguucucuuuucuucuuaacggucuaucacccuggaaacaguuu
gucuggagauaggguuuaauggccggaagagcccgacacuucugucgggu
ccggugcgcucucgacgucccuugaaaauccgcgggagggaauaauucuc
acgccaggucguacucauaaccgcagcagguccccaaggugaacagccuc
ugguugauagaacaauguagauaagggaagucggcaaaauagauccguaa
cuucgggaaaaggauuggcucuaaggguugggcacguugcggcgugcggu
uaacaaccaacuuagaacugguacggacagggggaaucugacugucuaau
uaaaacauagcauugcgauggccagaaagugguguugacgcaaugugauu
ucugcccagugcucugaaugucaaagugaagaaauucaaccaagcgcggg
uaaacggcgggaguaacuaugacucucuuaagguagccaaaugccucguc
aucuaauuagugacgcgcaugaauggauuaacgagauucccacugucccu
aucuacuaucuagcgaaaccacagccaagggaacgggcuuggcaaaauca
gcggggaaagaagacccuguugagcuugacucuaguuugacauugugaaa
agacauaggagcuccuauuguuuuuuuacuuauucaaugaagcggggcug
gacuugcguccaacuucuggaguuaagguccuucgcgggccgacccgggu
ugaagacauugucagguggggaguuuggcuggggcggcacaucuguuaaa
ccauaacgcagguguccuaaggggggcucauggagaacagaaaucuccag
uagaacaaaaggguaaaaguccccuugauuuugauuuucagugugaauac
aaaccaugaaaguguggccuaucgauccuuuagucccucgaaauuugagg
cuagaggugccagaaaaguuaccacagggauaacuggcuuguggcggcca
agcguucauagcgacgucgcuuuuugauccuucgaugucggcucuuccua
ucauaccgaagcagaauucgguaagcguuggauuguucacccacuaauag
ggaacgugagcuggguuuagaccgucgugagacagguuaguuuuacccua
cugaugaacucgucgcaaugguaauucagcuuaguacgagaggaaccgcu
gauucagauaauugguuuuugcgguuguccgaccgggcagugccgcgaag
cuaccaucugcuggauaauggcugaacgccucuaagucagaauccaugcc
agaacgcgacgauacuacccgcacguuguagacguauaagaauaggcucc
ggccucguauccuagcaggcgauuccuccgccggccucgaaguggccguc
gguaauucgcguauugcaauuuagacacgcgcgggaucaaauccuuugca
gacgacuuagaugugcgaaaggguccuguaagcaguagaguagccuuguu
guuacgaucugcugaggguaagcccuccuucgccuagauuu

.........................<<<<<<.....<......>......
>>>>>.>.........<<....>>...<<<<......<<.....>>....
.>>>>...[[[..<<.......<<.<<<....>>>.>>.......>>...
.<<<<<.<<<<<<<<<..<<<<<<..<<....<<<<......>>>>.((.
.....<<<.....))>>>....>>..>>>.>>>..>>>>....>>>>>>>
>.>>........<<<<<<<........>>>>>>>.....(((((.<<<<<
<<)))))..]]]..................................<<..
..>>...............<<<<....>>>>...................
...............<<<<.<<<<<<.....<<.<<<<<<<<<.....<<
<<<<....>>>>>>......>>>>>>>>>.>>.<<<<<<<.<<<<<<<<<
<<<<<<.....<.<<<<<<<<......<<<<<....>>>>>.......>>
>>>>>>....>.>>>>>>>>>>>>>>>........<<...>>.....>>>
>>>>.....>>>>>>.>>>>..<<<<........>>>><<<<<<....<<
<<<<<<.<<<<..<<<...<<<<.....>>>>...>>>...<<....>>.
......<<<<<..<<<..>>>...>>>>>.<<<<<<<<...<<....>>.
..>>>.>>>.>>....>>>>.>>>>>>>>..<<.<...<<<<<<.....<
<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<.
.<<.<<........>>>>.>>>....>>>>>>>>>...<<.......>>.
.....>>>>>>......>.>>...<<<<<....<.<<<....>>>.><<<
<<<<<<.....<<.<<<<<<<.....<<<....<<<....<<<...<<<<
<<........>>>>>>.>>>....>>>..>>.>.....>>>>>...<<<<
.<<<<.<......>>>>>.>>>>.....>>>>...>>>>>>>.>>..<<<
<.<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<..
.<<<(((.....<<<<<<.....<<..........<<<.<<<<<<<<<<.
......<<<<<.<<<...<<.........>>...>>>.........<<..
....>>...>>>>>...>>>>>>>>>>>>>..........))).>>...>
>>>>>.>>>...>>>>>>><<<<<<<<<.........>>>>>>>>>....
>>>>>.<.<<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>
>>>>>>>>>..>.....>>>>>><<.....<<.....<<<<<<.......
>>>>>><<<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>>>
>....((<<<<<<...<<<...<<<....>>>........<<<<<.....
.>>>>>....>>>....>>...<<<<<........>>>>>))<<<<.<<<
<.<<<....<<<....>>>.......<<<<<<...<<<<.<<<<<<<.<<
<<<.<<<<.......>>>>.><...<<<<<....<<<<<<....<<<...
..>>>....>>>>>>.....>>>>>....><<<<<<<......>>>>>>>
>>>>...>>>>>>>.>>>>.........>>>>>>.........>>>...>
>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>.
.>>>>..<<<<.....<<<<<<<.<<.........<.....<<<<<....
<<..>>.....>>>>><<.<<<<<<<<<<<.<<<<<<....>>>>>>...
...>>>>>..>>>>>>.>>............<<<<<<<<<..<<<.....
....>>>..<<<<<<<....<<<<.....>>>>......>>>>>>>.<.<
<..<<<..<<<<<<....<<<....<<<<<....>>>>.>...>>>.>>>
>..>>>>>.<<<<<.......>>>>>.........<...........<<<
<<.......>>>>>.....>.....>>.>..>>.>>>>>>>..>..>>.>
>>>>>>..>>>>>>....>>....<<<<<........>>>>>....[[[[
[..{{{.{{......[[[[.{{[[[[..<<<<<.<<<<<<<<..<<<<<<
<<<<<<<<<<..>>>>>.>>>>>>>>>>>...<<<<<.....<<<<..<<
<<<....>>>>>..............<<<<<.....>>>>>..>>>>..>
>>>>.....>>>>>>>>..>>>>>...<<<.....>>>..<<<<<<....
.......>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.
....<<...(((>>....>>>>>>>...<<.<<<<<<.......>>>>.>
>>>.<<<....>>>.>>))).......((((((..(((..>>>>>>>)))
))))))..........]]]]..}}]]]].......<<.<.<.<<<<<<..
.<<.......>>...>>>>>>...>>.>.>.......<<<<<..<<<<<.
.<.<<<<<<<......>>>>>>..>.>.<<<.........>>>.....>>
>>>......>>>.>>....<<<<<<<....>>>.>>>>....}}..}}}.
]]]]]....<<<<<<<..<<<<.<.<<<<<.................>>>
>>.><<<<<...<<<<....<<<<<<<<<<....>>>>>..>>>>>...>
>>>..>>>>>.<<<<...<<<<<...........>>>>>...>>>>.>>>
>....>>>>>>>.....<<<<<.<..<<<......<<.......<<<<..
>>>>....>>........<<<<.....<<<.<<<<<.......>>>>>.>
>>....>>>>..............>>>.>.>>>>>...<<<<<<<.....
........>>....<<<<<<<<<.<<<....<<<<.<..<<<<<......
>>>>>..>>>>>...>>>....>>>>..>>>>>...>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8oo0 Methionine aminopeptidase 2 and its autoproteolysis product have different binding sites on the ribosome.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): K13 K16 R17 Q22 D24 C28 R34 K35 K37 G38 I39 R45 R46 K48 S55 I56 G57 Y58 S60 K62 H66 A98 H99 N100 S102 R104 K105 K119
• Binding residue (residue number reindexed from 1): K12 K15 R16 Q21 D23 C27 R33 K34 K36 G37 I38 R44 R45 K47 S54 I55 G56 Y57 S59 K61 H65 A97 H98 N99 S101 R103 K104 K118

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8oo0, PDBe:8oo0, PDBj:8oo0
• PDBsum: 8oo0
• PubMed: 38267453
• UniProt: G0S6V4