Structure of PDB 7bhp Chain La Binding Site BS01

Receptor Information

>7bhp Chain La (length=147) Species: 9606 (Homo sapiens)

PSRLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPG
YFGKVGMKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPII
DVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGACVLVA

Ligand information

>7bhp Chain L5 (length=3644)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggacau
guggcguacggaagacccgcuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcccgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccggaggauucaacccggcggcggguccggccgugucggcgg
cccggcggaucuuucccgcgcgggggaccgucccccgaccggcgaccggc
cgccgccgggcgcauuuccaccgcggcggugcgccgcgaccggcuccggg
acggcugggaaggcccggcggggaagguggcucgggggcccccgaguguu
acagcccccccggcagcagcacucgccgaaucccggggccgagggagcga
gacccgucgccgcgcucuccccccucccggcggggggggccgggccaccc
cucccacggcgcgaccgcucggggcggacuguccccagugcgccccgggc
gggucgcgccgucgggcccgggggaggccacgcgcgcgucccccgagggg
gacggcggagcgagcgcacggggucggcggcgacgucggcuacccacccg
acccgucuugaaacacggaccaaggagucuaacacgugcgcgagucgggg
gcucgcacgaaagccgccguggcgcaaugaaggugaaggccggcgcgcuc
gccggccgaggugggaucccgaggccucuccaguccgccgagggcgcacc
accggcccgucucgcccgccgcgccggggagguggagcacgagcgcacgu
guuaggacccgaaagauggugaacuaugccugggcagggcgaagccagag
gaaacucugguggagguccguagcgguccugacgugcaaaucggucgucc
gaccuggguauaggggcgaaagacuaaucgaaccaucuaguagcugguuc
ccuccgaaguuucccucaggauagcuggcgcucucgcagacccgacccgc
cacgcaguuuuauccgguaaagcgaaugauuagaggucuuggggccgaaa
gaucucaaccuauucucaaacuuuaaauggguaagaagcccggcucgcug
gcguggagccgggcguggaaugcgagugccuagugggccacuuuugguaa
gcagaacuggcgcugcgggaugaaccgaacgccggguuaaggcgcccgau
gccgacgcucaucagaccccagaaaagguguugguugauauagacagcag
gacgguggcaagucgcuaaggagguguaacaacucaccugccgaaucaac
uagcccugaaaauggauggcgcuggagcgucgggcccauacccggccguc
gccggcagucgagaguggacgggagcgggcggcgcgccgcgcccggagcc
ccgcggacgcuacgccgcgacgaguaggagggccgcugcggugagccuug
aagccuagggcgcgggcccggguggagccgccgcaggugcagaucuuggu
gguaguagcaaauauucaaacgagaacuuugaaggccgaaguggagaagg
guuccaugugaacagcaguugaacaugggucagucgguccugagagaugg
gcgagcgccguuccgaagggacgggcgauggccuccguugcccucggccg
aucgaaagggagucggguucagauccccgaauccggaguggcggagaugg
gcgccgcgaggcguccagugcgguaacgcgaccgaucccggagaagccgg
cgggagccccggggagaguucucuuuucuuugugaagggcagggcgcccu
ggaauggguucgccccgagagaggggcccgugccuuggaaagcgucgcgg
uuccggcggcguccggugagcucucgcuggcccuugaaaauccgggggag
aggguguaaaucucgcgccgggccguacccauauccgcagcaggucucca
aggugaacagccucuggcauguuggaacaauguagguaagggaagucggc
aagccggauccguaacuucgggauaaggauuggcucuaagggcugggucg
gucgcggccggcgccuagcagccgacuuagaacuggugcggaccagggga
auccgacuguuuaauuaaaacaaagcaucgcgaaggcccgcggcgggugu
ugacgcgaugugauuucugcccagugcucugaaugucaaagugaagaaau
ucaaugaagcgcggguaaacggcgggaguaacuaugacucucuuaaggua
gccaaaugccucgucaucuaauuagugacgcgcaugaauggaugaacgag
auucccacugucccuaccuacuauccagcgaaaccacagccaagggaacg
ggcuuggcggaaucagcggggaaagaagacccuguugagcuugacucuag
ucuggcacggugaagagacaguuuuuucacugacccggugaggcgggggg
gcgagccccgaggggcucucgcuucuggcgccaagcgcccggccgcgcgc
cggccgggcgcgacccgcuccggggacagugccagguggggaguuugacu
ggggcgguacaccugucaaacgguaacgcagguguccuaaggcgagcuca
gggaggacagaaaccucccguggagcagaagggcaaaagcucgcuugauc
uugauuuucaguacgaauacagaccgugaaagcggggccucacgauccuu
cugaccuuuuggguuuuaagcaggaggugucagaaaaguuaccacaggga
uaacuggcuuguggcggccaagcguucauagcgacgucgcuuuuugaucc
uucgaugucggcucuuccuaucauugugaagcagaauucaccaagcguug
gauuguucacccacuaauagggaacgugagcuggguuuagaccgucguga
gacagguuaguuuuacccuacugaugauguguuguugccaugguaauccu
gcucaguacgagaggaaccgcagguucagacauuugguguaugugcuugg
cugaggagccaauggggcgaagcuaccaucugugggauuaugacugaacg
ccucuaagucagaaucccgcccaggcggaacgauacggcagcgccgcgga
gccucgguuggccucggauagccggucccccgccgggguccggugcggag
ugcccuucguccugggaaacggggcgcggccggagaggcggccgcccccu
cgcccgucacgcaccgcacguucguggggaaccuggcgcuaaaccauucg
uagacgaccugcuucugggucgggguuucguacguagcagagcagcuccc
ucgcugcgaucuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<.....<<....>>......
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>>>.>>.>>>>>>...>>>>>>>>>.......<<<<<<<........>>>
>>>>.....<<<<<..((((((>>>>>..>>>..................
................<<....>>...............<<<<....>>>
>..................................<<<<<<<<..<<<.<
...<.<<<<<<<.<<<.....<<<<<<<<<<<..<<..<<<..<......
....<<<<.<<<............>>>>>>>.....>..>>>.....>>>
>>>>>>>>>>......>>>.>>>.>>>>>...<<<<<<<<<<<<<<<<<<
.<<<<...<<..<<<.<<.<<<<......<<<<<<<....>>>>>>>...
....>>>>.>>>>>........>>>>>>...>>>>>>>>>>.........
...........<<<<<<.<<<<<...........>>>>>.<<<<<<.<<<
<.<<<<<<<<<<<.<<<<..<<<...<...........>....>>>.>.>
>>.>.>>>>>>>.>>>...>>>>..>>>>.>>..<<<<<<<<<<..>>>>
>>>>.>>....>>>>>>.....>>>.>>>>>....>>>>...>>>>>>>>
..<<<<........>>>><<<<<.....<<<<<<<<<<<<<..<<<...<
<<<........>>>>.....>>>...<<....>>...<<<<<<.......
.>>>>>>..<<<<<...<<<..<<<.......(((.>>>..>>>....>>
>>>.<<<<<<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>
>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<
....>>>>>>>.....>>>...<<<..<<.<<........>>>>.>>>..
..>>>>>>>>>...<<.......>>......>>>>>>......>.>>..<
<<<<<....<.<<<....>>>.><<.<<<<<<<.................
...<<<.<<<<<<<<<...<<<....<<<..<.<<<....<<<<......
..>>>>..>>>.>..>>>..>.>>...>>>>>>>...<<<<<<<<<....
.....>>>>>>>>>....>>>>>>>>>>>>..>>..<<<<.<<<<<....
.>>>>>.>>>>....<<<......>>>...<<<<<<<...<<<(((....
...<<<<<.....<<..........<<<.<<<<<<<<<<.......<<<<
.....................................>>>>...>>>>>>
>>>>>>>..........))).>>...>>>>>..>>>...>>>>>>><<<<
<<.<<<...........)))..............................
.............>>>>>>>>>....>>>>>>...<<<<<<<<.<<....
..<<<<.<<<<....>>>>>>>>...>>>>>>>>>>.........>>>>>
<<<....<<.....<<<<<<.......>>>>>>.<<<.....<<<<<.<<
...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<....<
<<....>>>..........<<.<<<<<<<<.....>>>>>>>>>>.....
.>>....>>...<<<<<........>>>>>))<<<<....<<<<<..<<<
<<<<<....>>>>>>>>..<<<....>>>......<<<<<<<...<<<<<
<<<<<<<.<<<<<<<<<<..[[[..>>>>>>....<<<<<.<<<<.<<<<
.....<<<.....>>>.....>>>>>>>>..>>>>>.....<<<<<<<..
.....>>>>>>>>>>>...>>>>>>>>>>>>.........>>>>>>>...
..........>>.>>>.>>>>>>>>..>>.....<<......>>>>>>>.
<<<<.....>>>>..>>>....<<<<.....<<<<<<<.<<.........
<.....<<<<<...............>>>>><<.<<<<<<<<<<<.....
..................>>>>>..>>>>>>.>>............<<<<
<<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>>>..
....>>>>>>>...<<..<<<..<<<<<<....<<<.....<<<<....>
>>>.....>>>.>>>>..>>>>>.<<<<.........>>>>.........
<...........<<<<<.......>>>>>.....>.....>>....>>.>
>>>>>>..>..>>.>>>>>>>..>>>>>>...>>>....<<<<<......
..>>>>>....<<<<<..<<<.<<......<<<<.<<<<<<..<<<<<.<
<<<<<<<<.<<<<<<<.......>>>>>>>...<<<<<.....<<<<...
.....<<......>>...................<<<<<<<<<<......
.>>>>>>>>>>..>>>>..>>>>>....>>>>>>>>>..>>>>>...<<<
.....>>>..<<<<<<...........>>>>>>..<<...<<<<<<<...
<<<<<<.......>>>>>>.....<<.[.(((>>....>>>>>>>...<<
<..<<<<.......>>>>..>>><<<<....>>>>>>)))].....<<<<
<<<..<<<..)))))).>>>>>>>>>>.........>>>>..>>>>>>..
.....<<.<...<<<<<<...<<.......>>...>>>>>>....>.>.>
.......<<<<<.<<<<<<..<.<.<<<<<......>>>>>...>.>.<<
<.........>>>.....>>>>>..>...>>>.>>....<<<<<<<....
>>>.>>>>....>>..>>>.>>>>>.....<<<<<<.<..<<<....<<<
<<.................>>>>>..<<<<....<<<<...<<<.<<<<<
<<]]].>>>>>..>>.>>>..>>>>...>>>><<<<....<<<<<.....
......>>>>>....>>>>.>>>...>.>>>>>>.....<<<<<<<.<<.
.<<<<....<<<........<<<<<.<<<<....>>>>.>>>>>......
.>>>...<<<...<<<....<<<<.<<<<<<<......>>>>>>>>>>>.
..>>>...>>>..............>>>>..>>>>>>>>><<<<<.<...
..........>....<<.<<<<<<<.<<.....<<<<....<<<<<....
..>>>>>...>>>>....>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7bhp Dynamic association of human Ebp1 with the ribosome.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): P2 S3 R4 R6 K7 T8 R9 K10 R12 G13 H14 V15 H17 G18 H19 G20 R21 I22 G23 K24 H25 R26 H28 G30 G31 R32 G33 N34 H39 H40 H41 R42 I43 N44 K47 F53 G54 K55 G57 M58 K59 H60 Y61 H62 L63 K64 R65 N66 N74 V82 S83 Q85 I101 D102 R105 K110 L112 G113 K114 K116 K119 S130 R131 R132 K136
• Binding residue (residue number reindexed from 1): P1 S2 R3 R5 K6 T7 R8 K9 R11 G12 H13 V14 H16 G17 H18 G19 R20 I21 G22 K23 H24 R25 H27 G29 G30 R31 G32 N33 H38 H39 H40 R41 I42 N43 K46 F52 G53 K54 G56 M57 K58 H59 Y60 H61 L62 K63 R64 N65 N73 V81 S82 Q84 I100 D101 R104 K109 L111 G112 K113 K115 K118 S129 R130 R131 K135

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005654 nucleoplasm
• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0016020 membrane
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7bhp, PDBe:7bhp, PDBj:7bhp
• PDBsum: 7bhp
• PubMed: 33479117
• UniProt: P46776|RL27A_HUMAN Large ribosomal subunit protein uL15 (Gene Name=RPL27A)