Structure of PDB 8g5y Chain LU Binding Site BS01

Receptor Information

>8g5y Chain LU (length=99) Species: 9606 (Homo sapiens)

VLKFTLDCTHPVEDGIMDAANFEQFLQERIKVNGKAGNLGGGVVTIERSK
SKITVTSEVPFSKRYLKYLTKKYLKKNNLRDWLRVVANSKESYELRYFQ

Ligand information

>8g5y Chain L5 (length=3721)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggacau
guggcguacggaagacccgcuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcccgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccggaggauucaacccggcggcggguccggccgugucggcgc
ggcggaucuuucccggcgggggaccgucccccgaccggcgaccggccgcc
gccgggcgcauuuccaccgcggcggugcgccgcgaccggcuccgggacgg
cugggaaggcccggcggggaagguggcucgggggcccccgaguguuacag
cccccccggcagcagcacucgccgaaucccggggccgagggagcgagacc
cgucgccgcgcucuccccccugggggggccgggccaccccucccacggcg
cgaccgcucggggcggacuguccccagugcgccccgggcgggucgcgccg
ucgggcccggggggccacgcgcgcgucccccgaagagggggacggcggag
cgagcgcacggggucggcggcgacgucggcuacccacccgacccgucuug
aaacacggaccaaggagucuaacacgugcgcgagucgggggcucgcacga
aagccgccguggcgcaaugaaggugaaggccggcgcgcucgccggccgag
gugggaucccgaggccucuccaguccgccgagggcgcaccaccggcccgu
cucgcccgccgcgccggggagguggagcacgagcgcacguguuaggaccc
gaaagauggugaacuaugccugggcagggcgaagccagaggaaacucugg
uggagguccguagcgguccugacgugcaaaucggucguccgaccugggua
uaggggcgaaagacuaaucgaaccaucuaguagcugguucccuccgaagu
uucccucaggauagcuggcgcucucgcagacccgacgccacgcaguuuua
uccgguaaagcgaaugauuagaggucuuggggccgaaacgaucucaaccu
auucucaaacuuuaaauggguaagaagcccggcucgcuggcguggagccg
ggcguggaaugcgagugccuagugggccacuuuugguaagcagaacuggc
gcugcgggaugaaccgaacgccggguuaaggcgcccgaugccgacgcuca
ucagaccccagaaaagguguugguugauauagacagcaggacgguggcca
uggaagucggaauccgcuaaggaguguguaacaacucaccugccgaauca
acuagcccugaaaauggauggcgcuggagcgucgggcccauacccggccg
ucgccggcagucgagaguggacgggagcggcgggcccgcggacgcuacgc
cgcgacgaguaggagggccgcugcggugagccuugaagccuagggcgcgg
gcccggguggagccgccgcaggugcagaucuuggugguaguagcaaauau
ucaaacgagaacuuugaaggccgaaguggagaaggguuccaugugaacag
caguugaacaugggucagucgguccugagagaugggcgagcgccguuccg
aagggacgggcgauggccuccguugcccucggccgaucgaaagggagucg
gguucagauccccgaauccggagcggagaugggcgccgcgaggcguccag
ugcgguaacgcgaccgaucccggagaagccggcgggagccccggggagag
uucucuuuucuuugugaagggcagggcgcccuggaauggguucgccccga
gagaggggcccgugccuuggaaagcgucgcgguuccggcggcguccggug
agcucucgcuggcccuugaaaauccgggggagaggguguaaaucucgcgc
cgggccguacccauauccgcagcaggucuccaaggugaacagccucuggc
auguuggaacaauguagguaagggaagucggcaagccggauccguaacuu
cgggauaaggauuggcucuaagggcugguagcagccgacuuagaacuggu
gcggaccaggggaauccgacuguuuaauuaaaacaaagcaucgcgaaggc
ccgcggcggguguugacgcgaugugauuucugcccagugcucugaauguc
aaagugaagaaauucaaugaagcgcggguaaacggcgggaguaacuauga
cucucuuaagguagccaaaugccucgucaucuaauuagugacgcgcauga
auggaugaacgagauucccacugucccuaccuacuauccagcgaaaccac
agccaagggaacgggcuuggcggaaucagcggggaaagaagacccuguug
agcuugacucuagucuggcacggugaagagacaugagagguguagaauaa
gugggaggcccccggcgcccccccgguguccccgcgaggggcccggggcg
ggguccgccggcccugcgggccgccggugaaauaccacuacucugaucgu
uuuuucacugacccggugaggcgggggggcgagccccgaggggcucucgc
uucuggcgccaagcgcccggccgcgcgccggccgggcgcgacccgcuccg
gggacagugccagguggggaguuugacuggggcgguacaccugucaaacg
guaacgcagguguccuaaggcgagcucagggaggacagaaaccucccgug
gagcagaagggcaaaagcucgcuugaucuugauuuucaguacgaauacag
accgugaaagcggggccucacgauccuucugaccuuuuggguuuuaagca
ggaggugucagaaaaguuaccacagggauaacuggcuuguggcggccaag
cguucauagcgacgucgcuuuuugauccuucgaugucggcucuuccuauc
auugugaagcagaauucaccaagcguuggauuguucacccacuaauaggg
aacgugagcuggguuuagaccgucgugagacagguuaguuuuacccuacu
gaugauguguuguugccaugguaauccugcucaguacgagaggaaccgca
gguucagacauuugguguaugugcuuggcugaggagccaauggggcgaag
cuaccaucugugggauuaugacugaacgccucuaagucagaaucccgccc
aggcggaacgauacggcagcgccgcggagccucgguuggccucggauagc
cggucccccgcgggguccggugcggagugcccuucguccugggaaacggg
gcgcggccggagaggcggccgcccccucgcccgucacgcaccgcacguuc
guggggaaccuggcgcuaaaccauucguagacgaccugcuucugggucgg
gguuucguacguagcagagcagcucccucgcugcgaucuauugaaaguca
gcccucgacacaaggguuugu

.........................<<<<<<.....<......>......
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..>>>>...<<<<<<<<<<...<<<<<<<<.<<.....<<<<<<<<....
<<<<......>>>>.....((......<<<.....))>>>...>.>>>>>
>>.>>>>>>>>>>..>>>>>>>>>>.......<<<<<<<........>>>
>>>>.....<<<<<..((((((>>>>>..>>>..................
................<<....>>...............<<<<....>>>
>..................................<<<<<<<<..<<<.<
<...<<<<<<<<.<<<.....<<<<<<<<<<<..<<<<<<<...<<<...
<<<<<.<<<..<<<...>>>>>>>>>>>..>>>..>>>>...>>>>>>>>
>>>>>>......>>>.>>>.>>>>>...<<<<<<<.<<<<<<<<<<<<<<
<...<<..<<<..<.<<<<......<<<<<<<<..>>>>>>>>.......
>>>>.>.>>>........>>>>>>..>>>>>>>>>>>.............
.......<<<<<<.<<<<<<..>>>>>>.<<<<<<.<<<<.<<<<<<<<<
<<<<<<<.<<<<<<<.<<<<....>>>>.>>>>>>>.>>>.>>>>>>>>>
>.>>>...>>>>>>>>.>>..<<<<<<<<<<.....>>>>>>>>.>>...
.>>>>>>......>>.>>>>>...>>>>>...>>>>>>>>..<<<<....
....>>>><<<<<<....<<<<<<<<<<<<<..<<<...<<<<.......
.>>>>.....>>>...<<....>>...<<<<<<<<....>>>>>>>>..<
<<<<...<<<..<<<..((((.(((.>>>..>>>....>>>>>.<<<<<<
<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.
<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>
>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>>
>...<<.......>>......>>>>>>...>..>.>>..<<<<<<....<
.<<<....>>>.><<.<<<<<<<..................<<<.<<<<<
<<<<...<<<....<<<..<.<<<..<<<<<<.<<...>>.>>>>>>>>>
.>..>>>..>.>>...>>>>>>>...<<<<<<<<<.........>>>>>>
>>>....>>>>>>>>>>>>..>>..<<<<.<<<<<.....>>>>>.>>>>
....<<<......>>>...<<<<<<<...<<<(((.......<<<<<...
..<<..........<<<.<<<<<<<<<<........<<<<.<<....<<.
........>>....>>.........<<......>>...>>>>....>>>>
>>>>>>>>>..........))).>>...>>>>>..>>>...>>>>>>><<
<<<<.<<<...........))).)))).....................>>
>>>>>>>....>>>>>>...<<<<<<<<.<<......<<<<.<<<<....
>>>>>>>>...>>>>>>>>>>........>>>>>><<.....<<.....<
<<<<<.......>>>>>>.<<<.....<<<<<.<<...<<<<<<...<<.
....>>.>>>>>>....((<<<<<<...<<....<<<....>>>......
....<<.<<<<<<<<<...>>>>>>>>>>>......>>....>>>..<<<
<<........>>>>>))<<<<.<<<<<..<<<<<<<<....>>>>>>>>.
.<<<....>>>...<..<<<<<<<...<<<<<<<<<<<<.<<<<<<<<<<
..[[[..>>>>>>....<<<<<.<<<<.<<<<.....<<<.....>>>..
...>>>>>>>>..>>>>>.....<<<<<<<.......>>>>>>>>>>>..
.>>>>>>>>>>>>.........>>>>>>>..>..........>>.>>>.>
>>>.>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>
....<<<<.....<<<<<<<.<<...............<<<<<.......
........>>>>><<.<<<<<<<<<<<....>>>>>..>>>>>>.>>...
.........<<<<<<<<<..<<<.........>>>..<<<<<<<....<<
<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<....<<<.
....<<<<....>>>>.....>>>.>>>>..>>>>><<<<<<.......>
>>>>>........<...........<<<<<.......>>>>>.....>..
...>>....>>.>>>>>>>.....>>.>>>>>>>..>>>>>>....>>..
..<<<<<........>>>>>....<<<<<..<<<.<<......<<<<.<<
<<<<..<<<<<.<<<<<<<<<.<<<<<<<<<<<<.<<<<...........
<..<<.............................................
..................................>>..>.>>>>.>>.>>
>>>>>>>>...<<<<<.....<<<<..<<.<<............>>>>..
............<<<<<<<<<<<.....>>>>>>>>>>>..>>>>..>>>
>>....>>>>>>>>>..>>>>>...<<<.....>>>..<<<<<<......
.....>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>...
..<<.[.(((>>....>>>>>>>...<<<..<<<<.......>>>>..>>
><<<<....>>>>>>)))].....<<<<<<<..<<<..)))))).>>>>>
>>>>>.........>>>>..>>>>>>.......<<.<...<<<<<<...<
<.......>>...>>>>>>....>.>.>.......<<<<<.<<<<<<..<
.<.<<<<<......>>>>>...>.>.<<<.........>>>.....>>>>
>..>...>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>.>>
>>>.....<<<<<<.<..<<<....<<<<<.................>>>
>>..<<<<....<<<<....<<.<<<<<<<]]].>>>>>..>>.>>...>
>>>...>>>><<<<<...<<<<<...........>>>>>...>>>>>.>>
>...>.>>>>>>.....<<<<<<<.<<..<<<<....<<<........<<
<<<.<<<<...>>>>.>>>>>.......>>>...<<<..<<<<....<<<
<.<<<<<<<......>>>>>>>>>>>...>>>>..>>>............
..>>>>..>>>>>>>>><<<<<....................<<.<<<<<
<<.<<.....<<<<.<..<<<<<......>>>>>..>>>>>....>>...
.>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8g5y mRNA decoding in human is kinetically and structurally distinct from bacteria.
• Resolution: 2.29 Å
• Binding residue (original residue number in PDB): K48 N50 G51 S79 K80 R81 Y82 K84 Y85 K89 K92 R97 R101 V103 A104 K107 Y110 R113
• Binding residue (residue number reindexed from 1): K31 N33 G34 S62 K63 R64 Y65 K67 Y68 K72 K75 R80 R84 V86 A87 K90 Y93 R96

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0008201 heparin binding
• GO:0042802 identical protein binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0046632 alpha-beta T cell differentiation
• GO:0140236 translation at presynapse

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0005925 focal adhesion
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0045202 synapse
• GO:0070062 extracellular exosome
• GO:0098793 presynapse
• GO:0098978 glutamatergic synapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8g5y, PDBe:8g5y, PDBj:8g5y
• PDBsum: 8g5y
• PubMed: 37020024
• UniProt: P35268|RL22_HUMAN Large ribosomal subunit protein eL22 (Gene Name=RPL22)