Structure of PDB 8fl7 Chain LU Binding Site BS01

Receptor Information

>8fl7 Chain LU (length=102) Species: 9606 (Homo sapiens)

ALRYPMAVGLNKGHKVTKNVSKPRHSRRRGRLTKHTKFVRDMIREVCGFA
PYERRAMELLKVSKDKRALKFIKKRVGTHIRAKRKREELSNVLAAMRKAA
AK

Ligand information

>8fl7 Chain L3 (length=3409)

gcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguca
gcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaaca
gggaagagcccagcgccgaauccccgccccggggcgcgggacauguggcg
uacggaagacccgcuccccggcgccgcgggggcccaaguccuucugaucg
aggcccagcccguggacggugugaggccgguagcggcccccggcgcgccg
ggcccgggucuucccggagucggguugcuugggaaugcagcccaaagcgg
gugguaaacuccaucuaaggcuaaauaccggcacgagaccgauagucaac
aaguaccguaagggaaaguugaaaagaacuuugaagagagaguucaagag
ggcgugaaaccguuaagaggaacgggugggguccgcgcaguccgcccgga
ggauucaacccggcggcggccggccgugucggcggcccggcggaucuggg
accgucccccgaccggcgaccggccgccgccgggcgcauuuccaccgcgg
cggugcgccgcgaccggcuccgggacggcugggaaggcccggcggggaag
guggcucgggcccgaguguuacagcccccccggcagcagcacucgccgaa
ucccggggccgagggaggucgccgcgcucuccccccggggggccgggcca
ccccucccacggcgcgaccgcucggggcggacuguccccagugcgccccg
ggcgggucgcgccgucgggcccggggggccacgcgcgcgucccccgaaga
gggggacggcggagcgagcgcacggggucggcggcgacgucggcuaccca
cccgacccgucuuacacggaccaaggagucuaacacgugcgcgagucggg
ggcucgcacgaaagccgccguggcgcaaugaaggugaaggccgcggccga
ggugggaucccgaggccucuccaguccgccgagggcgcaccaccggcccg
ucucgcccgccgcgccggggagguggagcacgagcgcacguguuaggacc
cgaaagauggugaacuaugccugggcagggcgaagccagaggaaacucug
guggagguccguagcgguccugacgugcaaaucggucguccgaccugggu
auaggggcgaaagacuaaucgaaccaucuaguagcugguucccuccgaag
uuucccucaggauagcuggcgcucuccgcaguuuuauccgguaaagcuuu
aaauggguaagaagcccggcucgcuggcguggagccgggcguggaaugcg
agugccuagugggccacuuuugcagaacuggcgcugcgggaugaaccgaa
cgccggguuaaggcgcccgaugccgacgcucaucagaccccaaaaggugu
ugguugauauagacagcaggacgguggccauggaagucggaauccgcuaa
ggaguguguaacaacucaccugccgaaucaacuagcccugaaaauggaug
gcgcuggagcgucgggcccauacccggccgucgccggcagucgagagugg
acgggagcggcgcgcggacgcuacgccgcgacgaguaggagggccgcugc
ggugagccuugaagccuagggcgcgggcccggguggagccgccgcaggug
cagaucuuggugguaguagcaaauauucaaacgagaacuuugaaggccga
aguggagaaggguuccaugugaacagcaguugaacaugggucagucgguc
cugagagaugggcgagcgccguuccgaagggacgggcgauggccuccggc
ccugaucgaaagggagucggguucagauccccgaauccggaguggcggag
augggcgccgcgaggcguccagugcgguaacgcgaccgaucccggagaag
ccggcgggagccccggggagaguucucuuuucuuugugaagggcagggcg
cccuggaauggguucgccccgagagaggggcccgugccuuggaaagcguc
gcgguuccggcggcguccggugagcucucgcuggcccuugaaaauccggg
ggagaggguguaaaucucgcgccgggccguacccauauccgcagcagguc
uccaaggugaacagccucuggcauguuggaacaauguagguaagggaagu
cggcaagccggauccguaacuucgggauaaggauuggcucuaagggcugg
gucggucgggcuggggcgcgaagcggggucccguggaucgccccagccgg
ccggcgccuagcagccgacuuagaacuggugcggaccaggggaauccgac
uguuuaauuaaaacaaagcaucgcgaaggcccgcggcggguguugacgcg
augugauuucugcccagugcucugaaugucaaagugaagaaauucaauga
agcgcggguaaacggcaagugaacgagauucccacugucccuaccuacua
uccagcgaaaccacagccaagggcgggcuuggcggaaucagcggggaaag
aagacccuguugagcuugacucuagucuggcacggugaaguuuuuucacu
gacccggugaggcggggggcuucuggcgccaagcgcccggccgcgcgccg
gccgggcgcgacccgcuccggggacagugccagguggggaguuugacugu
acaccugucaaacgguaacgcagguguccuaaggcgagcucagggaggac
agaaaccucccguggagcagaagggcaaaagcucgcuugaucuugauuuu
caguacgaauacagaccgugaaagcggggccucacgauccuucugaccuu
uuggguuuuaagcaggaggugucagaaaaguuaccacagggaucuugugg
cggccaagcgagcgacgucgcuuuuuggaugucggcucuuccuaucauug
ugaagcagaauucaccaagcguuggauuguucacccacuaauagggaacg
ugagcuggguuuagaccgucgugagacagguuaguuuuacccuacugaug
auguguuguugccaugguaauccugcucaguacgagaggaaccgcagguu
cagacauuugguguaugugcuuggcugaggagccaauggggcgaagcuac
caucugugggauuaugacugaacgccucuaagucagaaucccgcccaggc
ggaacgauagcgccgcggagccucgguuggccucggauagccgguccccg
ggguccggugcggagugcccuucguccugggaaacggggcgcggccggag
aggcggccgcccccucgcccgucacgcaccgcacguucguggggaaccug
gcgcuaaaccauucguagacgaccugcuucugggucgggguuucguacgu
agcagagcagcucccucgcugcgaucuauugaaagucagcccucgacaca
aggguuugu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<<.....<<.....>>....>
>>>>>..<<<..<<<<<...<<<<<<<<<..>>>>>.>>>>.....>>>>
>..<<<<<<<<<<...<<<<<<<<.<<<<<<<<<<....<<<<......>
>>>.....((......<<<.....))>>>...>.>>>>>>>.>>>>>>>>
>>..>>>>>>>>>>.......<<<<<<<........>>>>>>>.....<<
<<<..((((((>>>>>..>>>.............................
.....<<....>>...............<<<......>>>..........
......................<<<<<<<<..<<<.<<...<<<<<<<<.
<<<.....<<<<<<<<<<<<<<<<<<.<<<<<.<<...<<<<<.<<...>
>>>>>>>>>>>>>>>>>...>>>>>>>>>>>>>>......>>>.>>>.>>
>>>...<<<<<<<<<<<<<<<<<<<<<<<...<<..<<<.<<.<<<<...
...<<<<<<..>>>>>>.......>>>>>>.>>>........>>>>>>..
>>>>>>>>>>>............<<<<<<.<<<<<..>>>>>.<<<<<<.
<<<<.<<<<<<<<<<..<<<<<<<<<<<<<...........>.>>>>>>>
>>>>>...>>>>>>>.>>>...>>>>>>>>.>>..<<<<<<<<<......
.>>>>>>>.>>....>>>>>>.....>>>.>>>>>...>>>>>...>>>>
>>>>..<<<<.....>>>><<<<<<....<<<<<<<<<<<<<..<<<...
<<<<........>>>>.....>>>...<<....>>...<<<<..>>>>..
<<<<<...<<<..<<<..((((.((((>>>..>>>....>>>>>.<<<<<
<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<
.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>
>>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>
>>...<<.......>>......>>>>>>...>..>.>>..<<<<<<....
<.<<<....>>>.><<.<<<<<<<...<<<.<<<<<<<<<...<<<.>>>
...>>>>>>>...<<<<<<<<<.<......>>>>>>>>>>....>>>>>>
>>>>>>..>>..<<<<.<<<<..>>>>.>>>>....<<<......>>>..
.<<<<<<<...<<<(((.......<<<<<.....<<........<<<.<<
<<<<<<<<.......<<<<<.<<<...<<.........>>...>>>....
.....<<......>>...>>>>>...>>>>>>>>>>>>>..........)
)).>>...>>>>>..>>>...>>>>>>><<<<<<.<<<..........))
)).)))).................>>>>>>>>>....>>>>>>.<.<<<<
<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>
.....>>>>>><<.....<<.....<<<<<<.......>>>>>>.<<<..
...<<<<<.<<...<<<<<....<<.....>>..>>>>>....((<<<<<
<...<<....<<<....>>>.............<<<<...........>>
>>...>>....>>>..<<<<<........>>>>>))<<<<....<<<<<.
.<<<<<<<<....>>>>>>>>..<<<....>>>...<..<<<<<<<...<
<<<<<<<<<<<.<<<<<<<<<<..[[[..>>>>>>....<<<<<.<<<<.
<<<<.....<<<.....>>>.....>>>>>>>>..>>>>>.....<<<<<
<<.......>>>>>>>>>>>...>>>>>>>>>>>>.........>>>>>>
>..>..........>>.>>>.>>>>.>>>..>>.....<<......>>>>
>>>.<<<<.....>>>>..>>>....<<<<.....<<<<<<<.<<.....
..........<<<<<.....<..>......>>>>><<.<<<<<<<<<<<.
<<<<<<<<.<<<<<<<<<....<<<<<..>>>>>....>>>>>>>>>>>>
>>>>>......>>>>>..>>>>>>.>>............<<<<<<<<<..
<<<.........>>>..<<<<<<<....<<<<.....>>>>......>>>
>>>>.......<<<..<<<<<<.<..<<<.....<<<<....>>>>....
.>>>>>>>>..>>>>>.......>>.>>>>>>>.....>>.>>>>>>>..
>>>>>>....>>...<<<<<<......>>>>>>...<<<<<..<<<....
....<<<<<.<<<<<<..<<<<<.<<<<<<<<<.<<<<<.....>>>>>.
..<<<<<.....<<<<................<<<<<<<<<<.......>
>>>>>>>>>..>>>>..>>>>>....>>>>>>>>>..>>>>>...<<.>>
..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<..
.....>>>>>>.....<<.[.(((>>....>>>>>>>...<<<.<<<<<.
......>>>>.>>>>.<<<....>>>.>>)))].....<<<<<<<..<<<
..)))))).>>>>>>>>>>.........>>>>..>>>>>>>.......<<
<<<<...<<..>>...>>>>>>.....((...<<<<.<<<<<<..<.<.<
<<<<......>>>>>...>.>.<<<.....))..>>>.....>>>>>..>
...>>>>......<<<<<<<....>>>.>>>>........>>>.>>>>>.
....<<<<<<.<..<<<..<.<<<<<.................>>>>>.>
<<<<....<<<<....<<.<<<<<<.]]]..>>>>..>>.>>...>>>>.
..>>>><<<<<...<<<<<...........>>>>>...>>>>>.>>>...
>.>>>>>>.<<<<<<.<<..<<<<....<<<........<<<<<.<<<<>
>>>.>>>>>.......>>>...<<<..<<<<....<<<<.<<<<<<<...
...>>>>>>>>>>>...>>>>..>>>..............>>>>..>>>>
>>>>.<<<<<.<.............>....<<.<<<<<<<.<<.....<<
<<....<<<<<......>>>>>...>>>>....>>....>>>.>>>>.>>
..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fl7 Principles of human pre-60 S biogenesis.
• Resolution: 2.55 Å
• Binding residue (original residue number in PDB): R4 H15 R25 H26 S27 R28 R29 R30 G31 R32 L33 T34 K35 H36 R41 Y53 K67 R68 R76 G78 H80 R82 K84 R85 E89
• Binding residue (residue number reindexed from 1): R3 H14 R24 H25 S26 R27 R28 R29 G30 R31 L32 T33 K34 H35 R40 Y52 K66 R67 R75 G77 H79 R81 K83 R84 E88

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0016020 membrane
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0045202 synapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fl7, PDBe:8fl7, PDBj:8fl7
• PDBsum: 8fl7
• PubMed: 37410842
• UniProt: Q9Y3U8|RL36_HUMAN Large ribosomal subunit protein eL36 (Gene Name=RPL36)