Structure of PDB 8fl0 Chain LT Binding Site BS01

Receptor Information

>8fl0 Chain LT (length=109) Species: 9606 (Homo sapiens)

SGRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVY
KAKNNTVTPGGKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRI
RVMLYPSRI

Ligand information

>8fl0 Chain L3 (length=1558)

aguccgcccggaggauucaacccggcggcggccggccgugucggccgacc
ggcgaccggccgccgccgggcgcauuuccaccgcggcggugcgccgcgac
cggcuccgggacggcugggaaggcccggcggggaagguggcucgggcccg
aguguuacagcccccccggcagcagcacucgccgaaucccggggccgagg
gagucgccgcgcucuccccccggggggccgggccaccccucccacggcgc
gcgccgucgggcccggggggccacgcgcgcgucccccgaagagggggacg
gcggagcgcacggggucggcggcgcuaacacgugcgcgagucgggggcgc
cgccguggcgcggugggaucccgaggccgccgagggcgcaccaccggccc
gucucggagguggagcacgagcgcacguguuagcccuccgaaguuucccu
caggauagcuggcgcucuccgcaguuuuauccgggguaagaagcccggcu
cgcuggcguggagccgggcguggaaugcgagugccuaguggccacuuuug
guaagcagaacuggcgcgggaugaaccgaacgccggguuaaggcgcccga
ugccgacgcucaucagaccccagaaaagguguugguugauauagacagca
ggacgguggccauggaagucggaauccgcuaaggaguguguaacaacuca
ccugccgaaucaacuagcccugaaaaauggcgcuggagcgucgggcccau
acccggccgucgccggcaguacgcuacgccgcgacgaguaggagggccgc
ugcggugagccuugaagccuagggcgcgggcccggguggagccgccgcag
gugcccuguugagcuugacucuagucgguggggaguuugggcgguacacc
uagguguccuaaggcgagcucagggaggacagaaaccucccguggagcag
aagggcaaaagcucgcuugaucuugauuuguacgaauacagaccgugaaa
gcggggccuaaguuaccacagggaggcuuguggcggccaagcgagcgacg
ucgcuuuuugauccuucgaugucggcucuuccuaucauugugaagcagaa
uucaccaagcguuggauuguucacccacuaauagggagcuggguuuagac
cgucgugagacagguuaguguuguugccaugguaauccugcucaguacga
gaggaaccgcagguucagacauuugguguaugugcuggggcgaagcuacc
aucugugggauuaugacugaacgccucuaagucagaaucccgcccaggcg
gaacgauagcgccgcggagccucgguuggccucggauagccgguccccgg
gguccggugcggagugcccuucguccugggaaacggggcgcggccggaga
ggcggccgcccccucgcccgucacgcaccgcacguucguggggaaccugg
cgcuaaaccauucguagacgaccugcuucugggucgggguuucguacgua
gcagagcagcucccucgcugcgaucuauugaaagucagcccucgacacaa
ggguuugu

...<<<<<<<<.<<<.....<<<<<<<<<<<<<<<<<<.<<<<..>>>>>
>>>...>>>>>>>>>>>>>>......>>>.>>>.>>>>>...<<<<<<<<
<<<<<<<<<<<<<<<...<<..<<<.<<.<<<<......<<<<<<..>>>
>>>.......>>>>>>.>>>........>>>>>>..>>>>>>>>>>>...
........<<<<<<.<<<<<..>>>>>.<<<<<<.<<<<.<<<<<<<<<.
.>>>>>>.>>>...>>>>>>>>.>>..<<<<<<<<<.......>>>>>>>
.>>>>>>>>.....>>>.>>>>>.<<<<<<<<<<<<<..<<<...<<..>
>.....>>>..<<<<<...<<<..<<<.>>>..>>>....>>>>>.<<<<
<<<<<.>>>>>>>.>>....>>>>>>>>>>>>><<<<<<......<<<..
..>>>..<<.<<<<<<<...<<<.<<<<<<<<.>>>>>>...<<<<<<<<
<.<......>>>>>>>>>>....>>>>>>>>>>>>..>>.<<<<.<<<<<
.....>>>>>.>>>>.<<<......>>>...<<<<<<<...<<<(((...
....<<<<<.....<<..........<<<.<<<<<<<<<<.......<<<
<<.<<<...<<.........>>...>>>.........<<......>>...
>>>>>...>>>>>>>>>>>>>.......))).>>...>>>>>..>>>...
>>>>>>><<<<<<.<<<..........>>>>>>>>>....>>>>>>...<
<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>
...<<<<<.<<<<<<..<<<<<.<<..>>..>>>>>..<<<<<<.<<<<<
..>>>>>.>>..<<<<<<<...<<<<<<.......>>>>>>.....<<..
....>>....>>>>>>>...<<<.<<<<<....>>>>.>>>><<<<....
>>>>.>>>>.>>>>..>>>>>>>........<<<<<<...<<..>>...>
>>>>>.................<<<<..<<<<<..<.<.<<<<<......
>>>>>...>.>.<<<.........>>>.....>>>>>>>>>......<<<
<<<<....>>>.>>>>...<<<<<<.<..<<<..<.<<<<<.........
........>>>>>.><<<<....<<<<....<<.<..>.>>...>>>>..
.>>>><<<<<...<<<<<...........>>>>>...>>>>>.>>>...>
.>>>>>>.<<<<<<.<<..<<<<....<<<........<<<<<.<<<<>>
>>.>>>>>.......>>>...<<<..<<<<....<<<<.<<<<<<<....
..>>>>>>>>>>>...>>>>..>>>..............>>>>..>>>>>
>>>.<<<<<.<.............>....<<.<<<<<<<.<<.....<<<
<.<..<<<<<......>>>>>..>>>>>....>>....>>>.>>>>.>>.
.>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fl0 Principles of human pre-60 S biogenesis.
• Resolution: 2.91 Å
• Binding residue (original residue number in PDB): S2 G3 R4 W6 K8 K15 R16 L18 R19 R22 H24 Y34 R36 G44 K54 T59 P60 V69 W71 K73 R76 H78 G79 N80 S81 R85 K87 R89 K95 H99 R100 R102
• Binding residue (residue number reindexed from 1): S1 G2 R3 W5 K7 K14 R15 L17 R18 R21 H23 Y33 R35 G43 K53 T58 P59 V68 W70 K72 R75 H77 G78 N79 S80 R84 K86 R88 K94 H98 R99 R101

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0016020 membrane
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0070062 extracellular exosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fl0, PDBe:8fl0, PDBj:8fl0
• PDBsum: 8fl0
• PubMed: 37410842
• UniProt: P18077|RL35A_HUMAN Large ribosomal subunit protein eL33 (Gene Name=RPL35A)