Structure of PDB 8y0u Chain LR Binding Site BS01

Receptor Information

>8y0u Chain LR (length=188) Species: 4932 (Saccharomyces cerevisiae)

ANLRTQKRLAASVVGVGKRKVWLDPNETSEIAQANSRNAIRKLVKNGTIV
KKAVTVHSKSRTRAHAQSKREGRHSGYGKRKGTREARLPSQVVWIRRLRV
LRRLLAKYRDAGKIDKHLYHVLYKESKGNAFKHKRALVEHIIQAKADAQR
EKALNEEAEARRLKNRAARDRRAQRVAEKRDALLKEDA

Ligand information

>8y0u Chain C1 (length=3221)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugguguuuugua
uuucacgccagcaucaguuuugguggcaggauaaauccauaggaauguag
cuugccucgguaaguauuauagccugugggaauacugccagcugggacug
aggacugcgacguaagucaaggaugcuggcauaaugguuauaugccgccc
gucuugaaacacggaccaaggagucuaacgucuaugcgaguguuugggug
uaaaacccauacgcguaaugaaagugaacguagguuggggccucgcaaga
ggugcacaaucgaccgauccugaugucuucggauggauuugaguaagagc
auagcuguugggacccgaaagauggugaacuaugccugaauagggugaag
ccagaggaaacucugguggaggcucguagcgguucugacgugcaaaucga
ucgucgaauuuggguauaggggcgaaagacuaaucgaaccaucuaguagc
ugguuccugccgaaguuucccucaggauagcagaagcucguaucaguuuu
augagguaaagcgaaugauuagagguuccggggucgaaaugaccuugacc
uauucucaaacuuuaaauauguaagaaguccuuguuacuuaauugaacgu
ggacauuugaaugaagagcuuuuagugggccauuuuugguaagcagaacu
ggcgaugcgggaugaaccgaacguagaguuaaggugccggaauacacgcu
caucagacaccacaaaagguguuaguucaucuagacagccggacgguggc
cauggaagucggaauccgcuaaggaguguguaacaacucaccggccgaau
gaacuagcccugaaaauggauggcgcucaagcguguuaccuauacucuac
cgucaggguugauaugaugcccugacgaguaggcaggcguggaggucagu
gacgaagccuagaccguaaggucgggucgaacggccucuagugcagaucu
uggugguaguagcaaauauucaaaugagaacuuugaagacugaagugggg
aaagguuccacgucaacagcaguuggacguggguuagucgauccuaagag
auggggaagcuccguuucaaaggccugauuuuaugcaggccaccaucgaa
agggaauccgguuaagauuccggaaccuggauauggauucuucacgguaa
cguaacugaauguggagacgucggcgcgagcccugggaggaguuaucuuu
ucuucuuaacagcuuaucaccccggaauugguuuauccggagaugggguc
uuauggcuggaagaggccagcaccuuugcuggcuccggugcgcuugugac
ggcccgugaaaauccacaggaaggaauaguuuucaugccaggucguacug
auaaccgcagcaggucuccaaggugaacagccucuaguugauagaauaau
guagauaagggaagucggcaaaauagauccguaacuucgggauaaggauu
ggcucuaagggucggguacgaucaacuuagaacugguacggacaagggga
aucugacugucuaauuaaaacauagcauugcgauggucagaaagugaugu
ugacgcaaugugauuucugcccagugcucugaaugucaaagugaagaaau
ucaaccaagcgcggguaaacggcgggaguaacuaugacucucuuaaggua
gccaaaugccucgucaucuaauuagugacgcgcaugaauggauuaacgag
auucccacugucccuaucuacuaucuagcgaaaccacagccaagggaacg
ggcuuggcagaaucagcggggaaagaagacccuguugagcuugacucuag
uuugacauugugaagagacauagaggguguagaauaagugggagcuucgg
cgccagugaaauaccacuaccuuuauaguuucuuuacuuauucaaugaag
cggagcuggaauucauuuuccacguucuagcauucaaggucccauucggg
gcugauccggguugaagacauugucagguggggaguuuggcuggggcggc
acaucuguuaaacgauaacgcagauguccuaaggggggcucauggagaac
agaaaucuccaguagaacaaaaggguaaaagcccccuugauuuugauuuu
cagugugaauacaaaccaugaaaguguggccuaucgauccuuuagucccu
cggaauuugaggcuagaggugccagaaaaguuaccacagggauaacuggc
uuguggcagucaagcguucauagcgacauugcuuuuugauucuucgaugu
cggcucuuccuaucauaccgaagcagaauucgguaagcguuggauuguuc
acccacuaauagggaacgugagcuggguuuagaccgucgugagacagguu
aguuuuacccuacugaugaauguuaccgcaauaguaauugaacuuaguac
gagaggaacaguucauucggauaauugguuuuugcggcugucugaucagg
cauugccgcgaagcuaccauccgcuggauuauggcugaacgccucuaagu
cagaauccaugcuagaacgcggugauuucuuugcuccacacaauauagau
ggauacgaauaaggcguccuuguggcgucgcugaaccauagcaggcuagc
aacggugcacuuggcggaaaggccuugggugcuugcuggcgaauugcaau
gucauuuugcguggggauaaaucauuuguauacgacuuagauguacaacg
ggguauuguaagcaguagaguagccuuguuguuacgaucugcugagauua
agccuuuguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>...{.....<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[..<<.......<<.<<<<<<...>>>>>>.>>.......>
>....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]....}..........................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<<................
......<<<<<<<.<<<<<<<<<<<<<<<.....<<<<<<<<<......<
<<<<<....>>>>>>.......>>>>>>>.>.>.>>>>>.>>>>>>>>>>
........<<<....>>>.....>>>>>>>....>>>>>>.>>>>>..<<
<<........>>>><<<<<<....<<<<<<.<<<<<<..<<<...<<<<.
....>>>>...>>>...<<....>>.......<<<<<..<<<<<....>>
>>>...>>>>>.<<<<<<<<...<<....>>...>>>.>>>.>>....>>
>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<....<
<<<<<<....>>>>>>>.....>>>...<<<..<<.<<........>>>>
.>>>....>>>>>>>>>...<<.......>>......>>>>>>...>..>
.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<....<<.<<<<
<<<.....<<<....<<<..<.<<<<<..<<<<<<.....>>>>>>>>>>
>.>..>>>..>.>>.....>>>>>...<<<<.<<<<.<......>>>>>.
>>>>.....>>>>..>>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>
>>>....<<<......>>>...<<<<<<<..<<<<(((.....<<<<<<.
....<<..........<<<.<<<<<<<<<<.......<<<<<.<<<...<
<.........>>...>>>.........<<......>>...>>>>>...>>
>>>>>>>>>>>..........))).>>...>>>>>>.>>>>..>>>>>>>
<<<<<<<<<.........>>>>>>>>>....>>>>>><<<<<<<<<<.<<
......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>.>>.....>>
>>>><<<....<<.....<<<<<<.......>>>>>><<<<.....<<<<
<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<.
...<<<....>>>........<<<<<<<.....>.>>>>>>....>>...
.>>...<<<<<........>>>>>))<<<<.<<<<<<<.....<<<....
>>>.......<<<<<<...<<<<.<<<<<<<.<<<<<<<<<<..{{{..>
>>>>><...<<<<<....<<<<<<....<<<.....>>>....>>>>>>.
....>>>>>....><<<<<<<......>>>>>>>>>>>...>>>>>>>.>
>>>.........>>>>>>..........>>>..>>>>>>>>>>>>..>>.
....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<.....
<<<<<<<.<<.........<.....<<<<<....<....>.....>>>>>
<<.<<<<<<<<<<<....>>>>>..>>>>>>.>>............<<<<
<<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>>>..
....>>>>>>>...<<..<<<..<<<<<<.....<<....<<<<<....>
>>>.>...>>..>>>>..>>>>><<<<<<.......>>>>>>........
<...........<<<<<.......>>>>>.....>.....>>....>>.>
>>>>>>..>..>>.>>>>>>>..>>>>>>...>>>....<<<<<......
..>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[..<<<<<.<
<<<<<<<..<<<<<<<<<<<<<<<<<..........<<.<..........
..............>.>>.>>>>>>>.>>>>>>>>>>...<<<<<.....
<<<<..<<<<<......>>>>>...............<<<<<<....>>>
>>>..>>>>..>>>>>.....>>>>>>>>..>>>>>...<<<.....>>>
..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<..
.....>>>>>>.....<<...(((>>....>>>>>>>...<<<.<<<<<.
......>>>>.>>>>.<<<....>>>.>>))).......((((((..(((
(.>>>>>>))))))))))..........]]]]..}}]]]].......<<.
<.<.<<<<<<...<<.......>>...>>>>>>...>>..>>.......<
<<<<.<<<<<<..<.<<<<<<<......>>>>>>>...>.<<<.......
..>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>
....}}..}}}.]]]]]....<<<<<<<<..<<<<.<.<<<<<<......
.........>>>>>>.><<<<<...<<<<...<<<<<<<<<<<}}}.>>>
>>..>>>>>>..>>>>..>>>>>.<<<<...<<<<<...........>>>
>>...>>>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<....
<<..........<<<<<<.....>>>>>>......>>.......<<<<<<
...<<<<<.<..<<<......>>>..>.>>>>>.>>>>>>..........
...>>.>>>.>>>>>>..<<<<<.<.............>.....<<<<<<
<<<.<<<....<<<<....<<<<<......>>>>>...>>>>...>>>..
..>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8y0u dormant ribosome with STM1
• Resolution: 3.59 Å
• Binding residue (original residue number in PDB): L4 R5 K8 R9 G18 K19 R20 K21 W23 P26 S37 R38 R42 K43 K46 T56 H58 S59 K60 S61 R62 R64 R74 H75 G77 Y78 G79 K80 R81 K82 G83 T84 R85 E86 A87 R88 L89 Q92 W95 L99 R100 R103 R104 R110 K114 H118 Y120 H121 Y124 K125 K128 N130 K135 I143
• Binding residue (residue number reindexed from 1): L3 R4 K7 R8 G17 K18 R19 K20 W22 P25 S36 R37 R41 K42 K45 T55 H57 S58 K59 S60 R61 R63 R73 H74 G76 Y77 G78 K79 R80 K81 G82 T83 R84 E85 A86 R87 L88 Q91 W94 L98 R99 R102 R103 R109 K113 H117 Y119 H120 Y123 K124 K127 N129 K134 I142

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8y0u, PDBe:8y0u, PDBj:8y0u
• PDBsum: 8y0u
• PubMed: 38698775
• UniProt: P0CX82|RL19A_YEAST Large ribosomal subunit protein eL19A (Gene Name=RPL19A)