Structure of PDB 8fl2 Chain LQ Binding Site BS01

Receptor Information

>8fl2 Chain LQ (length=128) Species: 9606 (Homo sapiens)

AALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKG
QILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAH
NVSSKNRKAIVERAAQLAIRVTNPNARL

Ligand information

>8fl2 Chain L3 (length=3495)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccggggcgcgggacauguggc
guacggaagacccgcuccccggcgccgcgggggcccaaguccuucugauc
gaggcccagcccguggacggugugaggccgguagcggcccccggcgcgcc
gggcccgggucuucccggagucggguugcuugggaaugcagcccaaagcg
ggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauagucaa
caaguaccguaagggaaaguugaaaagaacuuugaagagagaguucaaga
gggcgugaaaccguuaagagguaaacgggugggguccgcgcaguccgccc
ggaggauucaacccggcggcggccggccgugucggcggcccggcggaucu
gggaccgucccccgaccggcgaccggccgccgccgggcgcauuuccaccg
cggcggugcgccgcgaccggcuccgggacggcugggaaggcccggcgggg
aagguggcucgggcccgaguguuacagcccccccggcagcagcacucgcc
gaaucccggggccgagggagucgccgcgcucuccccccggggggccgggc
caccccucccacggcgcgcgccgucgggcccggggggccacgcgcgcguc
ccccgaagagggggacggcggagcgagcgcacggggucggcggcgacguc
ggcuacccacccgacccgucuugaaacacggaccaaggagucuaacacgu
gcgcgagucgggggcucgcacgaaagccgccguggcgcaaugaaggugaa
ggccgcggccgaggugggaucccgaggcccgccgagggcgcaccaccggc
ccgucucgcccgccgcgccggggagguggagcacgagcgcacguguuagg
acccgaaagauggugaacuaugccugggcagggcgaagccagaggaaacu
cugguggagguccguagcgguccugacgugcaaaucggucguccgaccug
gguauaggggcgaaagacuaaucgaaccaucuaguagcugguucccuccg
aaguuucccucaggauagcuggcgcucuccgcaguuuuauccgguaaagc
gaaugauuagaggucuuggggccggaucucaaccuauucucggguaagaa
gcccggcucgcuggcguggagccgggcguggaaugcgagugccuaguggg
ccacuuuugguaagcagaacuggcgcugcgggaugaaccgaacgccgggu
uaaggcgcccgaugccgacgcucaucagaccccagaaaagguguugguug
auauagacagcaggacgguggccauggaagucggaauccgcuaaggagug
uguaacaacucaccugccgaaucaacuagcccugaaaauggauggcgcug
gagcgucgggcccauacccggccgucgccggcagugacgcuacgccgcga
cgaguaggagggccgcugcggugagccuugaagccuagggcgcgggcccg
gguggagccgccgcaggugcagaucuuggugguaguagcaaauauucaaa
cgagaacuuugaaggccgaaguggagaaggguuccaugugaacagcaguu
gaacaugggucagucgguccugagagaugggcgagcgccguuccgaaggg
acgggcgauggccuccggcccucgaucgaaagggagucggguucagaucc
ccgaauccggaguggcggagaugggcgccgcgaggcguccagugcgguaa
cgcgaccgaucccggagaagccggcgggagccccggggagaguucucuuu
ucuuugugaagggcagggcgcccuggaauggguucgccccgagagagggg
cccgugccuuggaaagcgucgcgguuccggcggcguccggugagcucucg
cuggcccuugaaaauccgggggagaggguguaaaucucgcgccgggccgu
acccauauccgcagcaggucuccaaggugaacagccucuggcauguugga
acaauguagguaagggaagucggcaagccggauccguaacuucgggauaa
ggauuggcucuaagggcugggucggucgggcuggggcgcgaagcgggguc
ccguggaucgccccagccggccggcgccuagcagccgacuuagaacuggu
gcggaccaggggaauccgacuguuuaauuaaaacaaagcaucgcgaaggc
ccgggguguugacgcgaugugauuucugcccagugcucugaaugucaaag
ugaagaaauucaaugaagcgcggguaaacggcgggaguaacuaugacucu
cuuaagguagccaaaugccucgucaugugacgcgcaugaauggaugaacg
agauucccacugucccuaccuacuauccagcgaaaccacagccaagggaa
cgggcuuggcggaaucagcggggaaagaagacccuguugagcuugacucu
agucuggcacggugaagagacaugagagguguagaauaagugggaggcgg
ccgccggugaaauaccacuacucugaucguuuuuucacugacccggugag
gcggggggcuucuggcgccaagcgcccggccgcgcgccggccgggcgcga
cccgcuccggggacagugccagguggggaguuugguacaccugucaaacg
guaacgcagguguccuaaggcgagcucagggaggacagaaaccucccgug
gagcagaagggcaaaagcucgcuugaucuugauuuuguacgaauacagac
cgugaaagcggggccucacccuucugaccuuuuggguuuuaagcaggagg
aaguuaccacagggauaacuggcuuguggcggccaagcgagcgacgucgc
uuuuugauccuucgaugucggcucuuccuaucauugugaagcagaauuca
ccaagcguuggauuguucacccacuaauagggaacgugagcuggguuuag
accgucgugagacagguuaguuuuacccuacugaugauguguuguugcca
ugguaauccugcucaguacgagaggaaccgcagguucagacauuuggugu
augugcuuggcugaggagccaauggggcgaagcuaccaucugugggauua
ugacugaacgccucuaagucagaaucccgcccaggcggaacgauagcgcc
gcggagccucgguuggccucggauagccgguccccgggguccggugcgga
gugcccuucguccugggaaacggggcgcggccggagaggcggccgccccc
ucgcccgucacgcaccgcacguucguggggaaccuggcgcuaaaccauuc
guagacgaccugcuucugggucgggguuucguacguagcagagcagcucc
cucgcugcgaucuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<.....<<....>>......
>>>>>.>.........<<....>>..<<<<<<.....<<.....>>....
>>>>>>..<<<..<<<<<...<<<<<<<<<..>>>>>.>>>>.....>>>
>>..<<<<<<<<<<...<<<<<<<<.<<<<<<<<<<....<<<<......
>>>>.....((......<<<.....))>>>...>.>>>>>>>.>>>>>>>
>>>..>>>>>>>>>>.......<<<<<<<........>>>>>>>.....<
<<<<..((((((>>>>>..>>>............................
......<<....>>...............<<<......>>>.........
.........................<<<<<<<<..<<<.<<...<<<<<<
<<.<<<.....<<<<<<<<<<<<<<<<<<.<<<<<.<<...<<<<<.<<<
.>>>>>>>>>>>>>>>>>>>...>>>>>>>>>>>>>>......>>>.>>>
.>>>>>...<<<<<<<<<<<<<<<<<<<<<<<...<<..<<<.<<.<<<<
......<<<<<<..>>>>>>.......>>>>>>.>>>........>>>>>
>..>>>>>>>>>>>...........<<<<<<.<<<<<..>>>>>.<<<<<
<.<<<<.<<<<<<<<<..>>>>>>.>>>...>>>>>>>>.>>..<<<<<<
<<<.......>>>>>>>.>>....>>>>>>.....>>>.>>>>>...>>>
>>...>>>>>>>>..<<<<........>>>><<<<<<....<<<<<<<<<
<<<<..<<<...<<<<........>>>>.....>>>...<<....>>...
<<<<..>>>>..<<<<<...<<<..<<<..>>>..>>>....>>>>>.<<
<<<<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>.
.<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>
>>>>>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>
>>>>>...<<.......>>......>>>>>>......>.>>..<<<<<<.
.....<<<....>>>..<<<<<<<<<<...<<<.<<<<<<<<........
....<<....<<<..<<<<<<.<.>.>>>>>>>>>..>>..>>>>>>...
<<<<<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>>.>>>..<
<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<
...<<<(((.......<<<<<.....<<..........<<<.<<<<<<<<
<<.......<<<<<.<<<...<<.........>>...>>>.........<
<......>>...>>>>>...>>>>>>>>>>>>>..........))).>>.
..>>>>>..>>>...>>>>>>><<<<<<.<<<...........>>>>>>>
>>....>>>>>>.<.<<<<<<<<.<<......<<<<.<<<<....>>>>>
>>>...>>>>>>>>>>..>.....>>>>>><<.....<<.....<<<<<<
.......>>>>>>.<<<.....<<<<<.<<...<<<<<....<<.....>
>..>>>>>....((<<<<<<...<<....<<<....>>>...........
..<<<<...........>>>>....>>....>>>..<<<<<........>
>>>>))<<<<....<<<<<..<<<<<<<<....>>>>>>>>..<<<....
>>>...<..<<<<<<<...<<<<<<<<<<<<.<<<<<<<<<<..[[[..>
>>>>>....<<<<<.<<<<.<<<<.....<<<.....>>>.....>>>>>
>>>..>>>>>.....<<<<<<<.......>>>>>>>>>>>...>>>>>>>
>>>>>.........>>>>>>>..>..........>>.>>>.>>>>.>>>.
.>>.....<<......>>>>>>>.<<<<.....>>>>..>>>....<<<<
.....<<<<<<<.<<...............<<<<<.....<..>......
>>>>><<.<<<<<<<<<<<.<<<<<<<<.<<<<<<<<<....<<<<<..>
>>>>....>>>>>>>>>>>>>>>>>......>>>>>..>>>>>>.>>...
.........<<<<<<<<<..<<<.........>>>..<<<<<<<....<<
<<.>>>>......>>>>>>>.......<<<..<<<<<<....<<<.....
<<<<....>>>>.....>>>.>>>>..>>>>><<<<<<.......>>>>>
>.............<<<<..<<<<<<>>>>>>.>>>>...........>>
.>>>>>>>.....>>.>>>>>>>..>>>>>>....>>....<<<<<....
....>>>>>....<<<<<..<<<........<<<<<.<<<<<<..<<<<<
.<<<<<<<<<.<<<<<<<<<<<<.<<<<..........<<<<<..<<<.>
>>............>>>>>.>>>>.>>.>>>>>>>>>>...<<<<<....
.<<<<................<<<<<<<<<<.......>>>>>>>>>>..
>>>>..>>>>>....>>>>>>>>>..>>>>>..<<.<<<<<<<.......
......>>>>>>>>>...<<<<<<<...<<<<<<.......>>>>>>...
..<<...(((>>....>>>>>>>...<<<..<<<<.[[..>>>>..>>>.
<<<....>>>...))).]]<<<<<<<..<<<..)))))).>>>>>>>>>>
.>>>>..>>>>>>>.............<<<<<<...<<..>>...>>>>>
>.................<<<<.<<<<<<..<.<.<<<<<......>>>>
>.>...>.<<<.........>>>.....>>>>>..>...>>>>......<
<<<<<<....>>>.>>>>........>>>.>>>>>.....<<<<<<.<..
<<<..<.<<<<<.................>>>>>.><<<<....<<<<..
..<<.<<<<<<<]]].>>>>>..>>.>>...>>>>...>>>><<<<<...
<<<<<...........>>>>>...>>>>>.>>>...>.>>>>>>.<<<<<
<.<<..<<<<....<<<........<<<<<.<<<<>>>>.>>>>>.....
..>>>...<<<..<<<<....<<<<.<<<<<<<......>>>>>>>>>>>
...>>>>..>>>..............>>>>..>>>>>>>>.<<<<<.<..
...........>....<<.<<<<<<<.<<.....<<<<.<..<<<<<...
...>>>>>..>>>>>....>>....>>>.>>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fl2 Principles of human pre-60 S biogenesis.
• Resolution: 2.67 Å
• Binding residue (original residue number in PDB): R5 K14 K15 R16 T17 K18 K19 F20 R22 Q24 D26 R27 Y28 V29 K30 I31 N34 W35 R36 K37 I41 D42 N43 R44 R46 R47 R48 F49 K50 N57 I58 G59 Y60 G61 S62 N63 K64 K65 K67 H68 N81 K83 E98 A100 H101 N102 S104 S105 N107 R108 N124 N126 A127 R128
• Binding residue (residue number reindexed from 1): R4 K13 K14 R15 T16 K17 K18 F19 R21 Q23 D25 R26 Y27 V28 K29 I30 N33 W34 R35 K36 I40 D41 N42 R43 R45 R46 R47 F48 K49 N56 I57 G58 Y59 G60 S61 N62 K63 K64 K66 H67 N80 K82 E97 A99 H100 N101 S103 S104 N106 R107 N123 N125 A126 R127

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0016020 membrane
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fl2, PDBe:8fl2, PDBj:8fl2
• PDBsum: 8fl2
• PubMed: 37410842
• UniProt: P62910|RL32_HUMAN Large ribosomal subunit protein eL32 (Gene Name=RPL32)