Structure of PDB 8fky Chain LQ Binding Site BS01

Receptor Information

>8fky Chain LQ (length=128) Species: 9606 (Homo sapiens)

AALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKG
QILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAH
NVSSKNRKAIVERAAQLAIRVTNPNARL

Ligand information

>8fky Chain L3 (length=2585)

gcgaccucagaucagacguggcgacccgcugaaagcauauuagucagcgg
aggagaagaaacuaaccaggauucccucaguaacggcgagugaacaggga
agagcccagcgccgaauccccgccccgggggcgcgggacauguggcguac
ggaagacccgcuccccggcgccgcucguggggggcccaaguccuucugau
cgaggcccagcccguggacggugugaggccgguagcggcccccggcgcgc
cgggcccgggucuucccggagucggguugcuugggaaugcagcccaaagc
gggugguccaucuaaggcuaaauaccggcacgagaccgauagucaacaag
uaccguaagggaaaguugaaaagaacuuugaagagagaguucaagagggc
gugaaaccguuaagagguaaacgggugggguccgcgcaguccgcccggag
gauucaacccggcggcggccgccgccgggcgcauuuccaccgcggcggug
cgccgcgaccggcuccgggacggcugggaaggcccggcggggaagguggc
ucgggcccgaguguuacagcccccccggcagcagcacucgccgaaucccg
gggccgagggagcgccgcgcucuccggccgggccaggccacggagcgcac
ggggucggcggcgacgucggcuacccacccgacccgucuugaaacacgga
ccaaggagucuaacacgugcgcgagucgggggcucgcacgaaagccgccg
uggcgcaaggugggaucccgaggcccgccgagggcgcaccaccggcccgu
cucgcccggggagguggagcacgagcgcacguguuaggacccgaaagaug
gugaacuaugcaagccagaggaaacucugguggguauaggggcgaaagac
uaaccaucuaguagcugguucccuccgaaguuucccggauagcuggcgcu
cucgcgcaguuuuauccgguggguaagaagcccggcucgagccgggcgug
gaaugcgagugccuagugggccacuuuugguaagcagaacuggcgcugcg
ggaugaaccgaacgccggguuaaggcgcccgaugccgacgcucaucagac
cccagaaaagguguugguugauauagacagcaggacgguggccauggaag
ucggaauccgcuaaggaguguguaacaacucaccugccgaaucaacuagc
ccugaaaauggauggcgcuggagcgucgggcccauacccggccgucgccg
gcagucacgcuacgccgcgacgaguaggagggccgcugcggugagccuug
aagccuagggcgcgggcccggguggagccgccgcaggugcagaucuuggu
gguaguagcuucaaacgagaacuuugaaggccgaaguggagaaggguucc
auguacaugggucagucgguccuagggacgggcgauggccuccggcccgc
cggggagucggguucagauccccgaauccggaguggcggagaugggcgcc
gcgaggcguccagugcgguaacgcgaccgaucccggagaagccggcggga
gccccggggagaguucucuuuucuuugugaagggcagggcgcccuggaau
ggguucgccccgagagaggggcccgugccuuggaaagcgucgcgguuccg
gcggcguccggugagcucucgcuggcaauccgggggagaggguguaaauc
ucgcgccgggccguacccuccaaggugaacagccucuggcaauguaggua
agggaagucgggaauccgauucccacugucccuaccuacuauccagcgaa
accacagccaagggaacgggcuuggcggaaucagcggggagaagacccug
uugagcuugacucuagucuggcacggugaagagacaugagagguguagaa
uaagugggaggcggccgccggugaaauaccacuacucugaucguuuuuuc
cagugccagguggggaguuauccuucugaccuuuuggguuuuaagcagga
ggugucagaaaaguuaccacagggauaacuggcuuguggcggccaagcgc
gacgucgcuuuuugaugaugucggcucuuccuaucauugugaagcagaau
ucaccaagcguuggauuguucacccacuaauagggaacgugagcugggga
ccgucgugagacagguuagucccuacugaugguuguugccaugguaaucc
ugcucaguacgagaggaaccgcagguucagacauuugguggugcuuggcu
gaggagccaauggggcgaagcuaccaucugugggauuaugacugaacgcc
ucuaagucagaaucccgcccaggcggaacgauacagcgccgcggagccuc
gguuggccucggauagccgguccccgggguccggugcggagugcccuucg
uccugggaaacggggcgcggccggagaggcggccgcccccucgcccguca
cgcaccgcacguucguggggaaccuggcgcuaaaccauucguagacgacc
ugcuucugggucgggguuucguacguagcagagcagcucccucgcugcga
ucuauugaaagucagcccucgacacaaggguuugu

........................<<<<<<...............>>>>>
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>..<<<..<<<<<...<<<<<<<<<<.>>>>>>.>>>>.....>>>>>..
<<<<<<<<<<...<<<<<<<<.<<.....<<<<<<<<....<<<<.....
.>>>>.....((......<<<.....))>>>...>.>>>>>>>.>>>>>>
>>>>..>>>>>>>>>>.......<<<<<<<........>>>>>>>.....
<<<<<<.>>>>>>..>>>................................
..<<....>>...............<<<<....>>>>.............
.....................<<<<<<<<..<<<.<<...<<<<<<<<.<
<<.....<<<<<<<<<<..>>>>>>>>>>......>>>.>>>.>>>>>..
.<<<<<<<.<<<<<<<<<<<<<<<...<<..<<<.<<.<<<<......<<
<<<<..>>>>>>.......>>>>>>.>>>........>>>>>>..>>>>>
>>>>>>..........<<<<<<.<..>.<<<<<<.>>>>.>>>>>>>>..
....>>.>>>>>...>>>>>...>>>>>>>>..<<<<........>>>><
<<<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>....
.>>>....<<<<<...<<<..<<<..>>>..>>>....>>>>>.<<<<<<
<<<.<<..>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.<...<<<<
<<...<<<<<...<<<<<<<....>>>>>>>...>>>>><<.<....>.>
>.>>>>>>......>.>>..<<<<<<......<<<.>>>..<<.<<<<<<
<....<<<.<<<<<<<<...>>>>>>...<<<<<<<<..>>>>>>>>...
.>>>>>>>>>>>>..>>..<<<<.<<<<<.....>>>>>.>>>>....<<
<......>>>...<<<<<<<...<<<(((.......<<<<<.....<<..
........<<<.<<<<<<<<<<.....<.<<<<<.<<<...<<.......
..>>...>>>.........<<......>>...>>>>>.>.>>>>>>>>>>
>>>..........))).>>...>>>>>..>>>...>>>>>>><<<<<<.<
<<...........>>>>>>>>>....>>>>>>.<.<<<<<<<<.<<....
..<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>>>>
<<.....<<<<<<<<.......>>>>>>.<<<.....<<<<..<<...<<
<<<..>>>>>....((<<<<<...<<...<<<<...........>>>>.>
>..>...<<<<<........>>>>>))<<<<....<<<<<...<<<<<<<
....>>>>>>>...<<<....>>>......<<<<<<<...<<<<<<<<<<
<..<<<<<<<<<<.......>>>>>>.....<<<<.<<<<.<<<<.....
<<<.....>>>.....>>>>>>>>..>>>>......<<<<<<<.......
>>>>>>>>>>>....>>>>>>>>>>>..>>>>>>>.............>>
.>>>.>>>>>>>>..>>>>>>.<<<<.....>>>>..>>>...<<<<<<.
<<<<<<<<..<<<<<<.>>>>>>.>>>.>>>>>>>>>>>......>>...
.>>...<<<<<<........>>>>>>....<<<<.<<<<......<<<<<
<.<<<<<<.<<<<<.<<<<<<<<<...<<<<<<<<<<.<<<<........
..<<<<<..<<<.>>>............>>>>>.>>>>.>>.>>>>>>>>
..>>>>>>>>>..>>>>>...<<<<<<<..<<<.........>>>>>>>>
>>........>>>>>.>.>>>>>>.............<<<<<<...<..>
...>>>>>>.......((...<<<<<<<<<<<..<.<.<<<<<......>
>>>>...>.>.<<<.....))..>>>.....>>>>>....>>>>>>..<<
<<<<<....>>>.>>>>...>>>>.>>>>..<<<<<<.<..<<<..<.<<
<<<.................>>>>>.><<<<....<<<<.<<.<<<<<<<
....>>>>>..>>.>>...>>>>...>>>><<<<<...<<<<<.......
....>>>>>...>>>>>.>>>...>.>>>>>>..<<<<<<<.<<..<<<<
....<<<........<<<<<.<<<<>>>>.>>>>>.......>>>...<<
<..<<<<....<<<<.<<<<<<<......>>>>>>>>>>>...>>>>..>
>>..............>>>>..>>>>>>>>><<<<<.<............
.>....<<.<<<<<<<.<<.....<<<<.<..<<<<<......>>>>>..
>>>>>....>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fky Principles of human pre-60 S biogenesis.
• Resolution: 2.67 Å
• Binding residue (original residue number in PDB): R5 K14 K15 R16 T17 K18 F20 R22 D26 R27 Y28 V29 K30 I31 N34 W35 R36 R39 I41 N43 R46 R47 R48 F49 K50 P56 N57 I58 G59 Y60 G61 S62 N63 K64 K65 K67 H68 E98 A100 H101 N102 S104 S105 N107 R108 N124 A127 R128
• Binding residue (residue number reindexed from 1): R4 K13 K14 R15 T16 K17 F19 R21 D25 R26 Y27 V28 K29 I30 N33 W34 R35 R38 I40 N42 R45 R46 R47 F48 K49 P55 N56 I57 G58 Y59 G60 S61 N62 K63 K64 K66 H67 E97 A99 H100 N101 S103 S104 N106 R107 N123 A126 R127

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0016020 membrane
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fky, PDBe:8fky, PDBj:8fky
• PDBsum: 8fky
• PubMed: 37410842
• UniProt: P62910|RL32_HUMAN Large ribosomal subunit protein eL32 (Gene Name=RPL32)