Structure of PDB 8fl9 Chain LP Binding Site BS01

Receptor Information

>8fl9 Chain LP (length=106) Species: 9606 (Homo sapiens)

NEVVTREYTINIHKRIHGVGFKKRAPRALKEIRKFAMKEMGTPDVRIDTR
LNKAVWAKGIRNVPYRIRVRLSRKRNEDEDSPNKLYTLVTYVPVTTFKNL
QTVNVD

Ligand information

>8fl9 Chain L3 (length=3361)

gcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguca
gcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaaca
gggaagagcccagcgccgaauccccgccccggggcgcgggacauguggcg
uacggaagacccgcuccccggcgccgcgggggcccaaguccuucugaucg
aggcccagcccguggacggugugaggccgguagcggcccccggcgcgccg
ggcccgggucuucccggagucggguugcuugggaaugcagcccaaagcgg
gugguaaacuccaucuaaggcuaaauaccggcacgagaccgauagucaac
aaguaccguaagggaaaguugaaaagaacuuugaagagagaguucaagag
ggcgugaaaccguuaagaggaacgggugggguccgcgcaguccgcccgga
ggauucaacccggcggcggccggccgugucggcggcccggcggaucuggg
accgucccccgaccggcgaccggccgccgccgggcgcauuuccaccgcgg
cggugcgccgcgaccggcuccgggacggcugggaaggcccggcggggaag
guggcucgggcccgaguguuacagcccccccggcagcagcacucgccgaa
ucccggggccgagggaggucgccgcgcucuccccccggggggccgggcca
ccccucccacggcgcgaccgcucggggcggacuguccccagugcgccccg
ggcgggucgcgccgucgggcccggggggccacgcgcgcgucccccgaaga
gggggacggcggagcgagcgcacggggucggcggcgacgucggcuaccca
cccgacccgucuuacacggaccaaggagucuaacacgugcgcgagucggg
ggcucgcacgaaagccgccguggcgcaaugaaggugaaggccgcggccga
ggugggaucccgaggccucuccaguccgccgagggcgcaccaccggcccg
ucucgcccgccgcgccggggagguggagcacgagcgcacguguuaggacc
cgaaagauggugaacuaugccugggcagggcgaagccagaggaaacucug
guggagguccguagcgguccugacgugcaaaucggucguccgaccugggu
auaggggcgaaagacuaaucgaaccaucuaguagcugguucccuccgaag
uuucccucaggauagcuggcgcucuccgcaguuuuauccgguaaagcuuu
aaauggguaagaagcccggcucgcuggcguggagccgggcguggaaugcg
agugccuagugggccacuuuugcagaacuggcgcugcgggaugaaccgaa
cgccggguuaaggcgcccgaugccgacgcucaucagaccccaaaaggugu
ugguugauauagacagcaggacgguggccauggaagucggaauccgcuaa
ggaguguguaacaacucaccugccgaaucaacuagcccugaaaauggaug
gcgcuggagcgucgggcccauacccggccgucgccggcagucgagagugg
acgggagcggcgcgcggacgcuacgccgcgacgaguaggagggccgcugc
ggugagccuugaagccuagggcgcgggcccggguggagccgccgcaggug
cagaucuuggugguaguagcaaauauucaaacgagaacuuugaaggccga
aguggagaaggguuccaugugaacagcaguugaacaugggucagucgguc
cugagagaugggcgagcgccguuccgaagggacgggcgagcccugaucga
aagggagucggguucagauccccgaauccggaguggcggagaugggcgcc
gcgaggcguccagugcgguaacgcgaccgaucccggagaagccggcggga
gccccggggagaguucucuuuucuuugugaagggcagggcgcccuggaau
ggguucgccccgagagaggggcccgugccuuggaaagcgucgcgguuccg
gcggcguccggugagcucucgcuggcccuugaaaauccgggggagagggu
guaaaucucgcgccgggccguacccauauccgcagcaggucuccaaggug
aacagccucuggcauguuggaacaauguagguaagggaagucggcaagcc
ggauccguaacuucgggauaaggauuggcucuaagggcugggucggucgc
ggccggcgccuagcagccgacuuagaacuggugcggaccaggggaauccg
acuguuuaauuaaaacaaagcaucgcgaaggcccgcggcggguguugacg
cgaugugauuucugcccagugcucugaaugucaaagugaagaaauucaau
gaagcgcggguaaacggcaagugaacgagauucccacugucccuaccuac
uauccagcgaaaccacagccaagggcgggcuuggcggaaucagcggggaa
agaagacccuguugagcuugacucuagucuggcacggugaaguuuuuuca
cugacccggugaggcggggggcuucuggcgccaagcgcccggccgcgcgc
cggccgggcgcgacccgcuccggggacagugccagguggggaguuugacu
guacaccugucaaacgguaacgcagguguccuaaggcgagcucagggagg
acagaaaccucccguggagcagaagggcaaaagcucgcuugaucuugauu
uucaguacgaauacagaccgugaaagcggggccucacgauccuucugacc
uuuuggguuuuaagcaggaggugucagaaaaguuaccacagggaucuugu
ggcggccaagcgagcgacgucgcuuuuuggaugucggcucuuccuaucau
ugugaagcagaauucaccaagcguuggauuguucacccacuaauagggaa
cgugagcuggguuuagaccgucgugagacagguuaguuuuacccuacuga
ugauguguuguugccaugguaauccugcucaguacgagaggaaccgcagg
uucagacauuugguguaugugcuuggcugaggagccaauggggcgaagcu
accaucugugggauuaugacugaacgccucuaagucagaaucccgcccag
gcggaacgauagcgccgcggagccucgguuggccucggauagccgguccc
cgggguccggugcggagugcccuucguccugggaaacggggcgcggccgg
agaggcggccgcccccucgcccgucacgcaccgcacguucguggggaacc
uggcgcuaaaccauucguagacgaccugcuucugggucgggguuucguac
guagcagagcagcucccucgcugcgaucuauugaaagucagcccucgaca
caaggguuugu

........................<<<<<<.....<<....>>......>
>>>>.>.........<<....>>..<<<<<<.....<<.....>>....>
>>>>>..<<<..<<<<<...<<<<<<<<<..>>>>>.>>>>.....>>>>
>..<<<<<<<<<<...<<<<<<<<.<<<<<<<<<<....<<<<......>
>>>.....((......<<<.....))>>>...>.>>>>>>>.>>>>>>>>
>>..>>>>>>>>>>.......<<<<<<<........>>>>>>>.....<<
<<<..((((((>>>>>..>>>.............................
.....<<....>>...............<<<......>>>..........
......................<<<<<<<<..<<<.<<...<<<<<<<<.
<<<.....<<<<<<<<<<<<<<<<<<.<<<<<.<<...<<<<<.<<...>
>>>>>>>>>>>>>>>>>...>>>>>>>>>>>>>>......>>>.>>>.>>
>>>...<<<<<<<<<<<<<<<<<<<<<<<...<<..<<<.<<.<<<<...
...<<<<<<..>>>>>>.......>>>>>>.>>>........>>>>>>..
>>>>>>>>>>>............<<<<<<.<<<<<..>>>>>.<<<<<<.
<<<<.<<<<<<<<<<..<<<<<<<<<<<<<...........>.>>>>>>>
>>>>>...>>>>>>>.>>>...>>>>>>>>.>>..<<<<<<<<<......
.>>>>>>>.>>....>>>>>>.....>>>.>>>>>...>>>>>...>>>>
>>>>..<<<<.....>>>><<<<<<....<<<<<<<<<<<<<..<<<...
<<<<........>>>>.....>>>...<<....>>...<<<<..>>>>..
<<<<<...<<<..<<<..((((.((((>>>..>>>....>>>>>.<<<<<
<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<
.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>
>>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>
>>...<<.......>>......>>>>>>...>..>.>>..<<<<<<....
<.<<<....>>>.><<.<<<<<<<...<<<.<<<<<<<<<...<<<.>>>
...>>>>>>>...<<<<<<<<<.<......>>>>>>>>>>....>>>>>>
>>>>>>..>>..<<<<.<<<<..>>>>.>>>>....<<<......>>>..
.<<<<<<<...<<<(((.......<<<<<.....<<........<<<.<<
<<<<<<<<.......<<<<<.<<<...<<.........>>...>>>....
.....<<......>>...>>>>>...>>>>>>>>>>>>>..........)
)).>>...>>>>>..>>>...>>>>>>><<<<<<.<<<..........))
)).)))).................>>>>>>>>>....>>>>>>.<.<<<<
<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>
.....>>>>>><<.....<<.....<<<<<<.......>>>>>>.<<<..
...<<<<<.<<...<<<<<....<<.....>>..>>>>>....((<<<<<
<...<<....<<<....>>>.............<<<<..>>>>...>>..
..>>>..<<<<<........>>>>>))<<<<....<<<<<..<<<<<<<<
....>>>>>>>>..<<<....>>>...<..<<<<<<<...<<<<<<<<<<
<<.<<<<<<<<<<..[[[..>>>>>>....<<<<<.<<<<.<<<<.....
<<<.....>>>.....>>>>>>>>..>>>>>.....<<<<<<<.......
>>>>>>>>>>>...>>>>>>>>>>>>.........>>>>>>>..>.....
.....>>.>>>.>>>>.>>>..>>.....<<......>>>>>>>.<<<<.
....>>>>..>>>....<<<<.....<<<<<<<.<<..............
.<<<<<....<<..>>.....>>>>><<.<<<<<<<<<<<.<<<<<<<..
>>>>>>>......>>>>>..>>>>>>.>>............<<<<<<<<<
..<<<.........>>>..<<<<<<<....<<<<.....>>>>......>
>>>>>>..<....<<<..<<<<<<.<..<<<.....<<<<....>>>>..
...>>>>>>>>..>>>>>....>..>>.>>>>>>>.....>>.>>>>>>>
..>>>>>>....>>...<<<<<<......>>>>>>...<<<<<..<<<..
......<<<<<.<<<<<<..<<<<<.<<<<<<<<<.<<<<<.....>>>>
>...<<<<<.....<<<<................<<<<<<<<<<......
.>>>>>>>>>>..>>>>..>>>>>....>>>>>>>>>..>>>>>...<<.
>>..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<
.......>>>>>>.....<<.[.(((>>....>>>>>>>...<<<.<<<<
<.......>>>>.>>>>.<<<....>>>.>>)))].....<<<<<<<..<
<<..)))))).>>>>>>>>>>.........>>>>..>>>>>>>.......
<<<<<<...<<..>>...>>>>>>.....((...<<<<.<<<<<<..<.<
.<<<<<......>>>>>...>.>.<<<.....))..>>>.....>>>>>.
.>...>>>>......<<<<<<<....>>>.>>>>........>>>.>>>>
>.....<<<<<<.<..<<<..<.<<<<<.................>>>>>
.><<<<....<<<<....<<.<<<<<<<]]].>>>>>..>>.>>...>>>
>...>>>><<<<<...<<<<<...........>>>>>...>>>>>.>>>.
..>.>>>>>>.<<<<<<.<<..<<<<....<<<........<<<<<.<<<
<>>>>.>>>>>.......>>>...<<<..<<<<....<<<<.<<<<<<<.
.....>>>>>>>>>>>...>>>>..>>>..............>>>>..>>
>>>>>>.<<<<<....................<<.<<<<<<<.<<.....
<<<<....<<<<<......>>>>>...>>>>....>>....>>>.>>>>.
>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fl9 Principles of human pre-60 S biogenesis.
• Resolution: 2.75 Å
• Binding residue (original residue number in PDB): T26 N28 H30 K31 R32 H34 G35 F38 K39 R41 K47 R50 I64 T66 N69 W73 K75 I77 R78 N79 Y82 R83 N116 L117 Q118
• Binding residue (residue number reindexed from 1): T9 N11 H13 K14 R15 H17 G18 F21 K22 R24 K30 R33 I47 T49 N52 W56 K58 I60 R61 N62 Y65 R66 N99 L100 Q101

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0005925 focal adhesion
• GO:0016020 membrane
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0070062 extracellular exosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fl9, PDBe:8fl9, PDBj:8fl9
• PDBsum: 8fl9
• PubMed: 37410842
• UniProt: P62899|RL31_HUMAN Large ribosomal subunit protein eL31 (Gene Name=RPL31)