Structure of PDB 7n30 Chain LP Binding Site BS01

Receptor Information
>7n30 Chain LP (length=136) Species: 83333 (Escherichia coli K-12) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTARQIEAAR
RAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGK
VLYEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM
Ligand information
>7n30 Chain 23 (length=2901) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuaggc
gugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguag
caagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggc
guuaaguugcaggguauagacccgaaacccggugaucuagccaugggcag
guugaagguuggguaacacuaacuggaggaccgaaccgacuaauguugaa
aaauuagcggaugacuuguggcugggggugaaaggccaaucaaaccggga
gauagcugguucuccccgaaagcuauuuagguagcgccucgugaauucau
cuccggggguagagcacuguuucggcaagggggucaucccgacuuaccaa
cccgaugcaaacugcgaauaccggagaauguuaucacgggagacacacgg
cgggugcuaacguccgucgugaagagggaaacaacccagaccgccagcua
aggucccaaagucaugguuaagugggaaacgaugugggaaggcccagaca
gccaggauguuggcuuagaagcagccaucauuuaagaaagcguaauagcu
cacuggucgagucggccugcgcggaagauguaacggggcuaaaccaugca
ccgaagcugcggcagcgacgcuuaugcguuguuggguaggggagcguucu
guaagccugcgaaggugugcugugaggcaugcuggagguaucagaagugc
gaaugcugacauaaguaacgauaaagcgggugaaaagcccgcucgccgga
agaccaaggguuccuguccaacguuaaucggggcagggugagucgacccc
uaaggcgaggccgaaaggcguagucgaugggaaacagguuaauauuccug
uacuugguguuacugcgaaggggggacggagaaggcuauguuggccgggc
gacgguugucccgguuuaagcguguaggcugguuuuccaggcaaauccgg
aaaaucaaggcugaggcgugaugacgaggcacuacggugcugaagcaaca
aaugcccugcuuccaggaaaagccucuaagcaucagguaacaucaaaucg
uaccccaaaccgacacagguggucagguagagaauaccaaggcgcuugag
agaacucgggugaaggaacuaggcaaaauggugccguaacuucgggagaa
ggcacgcugauauguaggugaggucccucgcggauggagcugaaaucagu
cgaagauaccagcuggcugcaacuguuuauuaaaaacacagcacugugca
aacacgaaaguggacguauacggugugacgccugcccggugccggaaggu
uaauugaugggguuagcgcaagcgaagcucuugaucgaagccccgguaaa
cggcggccguaacuauaacgguccuaagguagcgaaauuccuugucgggu
aaguuccgaccugcacgaauggcguaaugauggccaggcugucuccaccc
gagacucagugaaauugaacucgcugugaagaugcaguguacccgcggca
agacggaaagaccccgugaaccuuuacuauagcuugacacugaacauuga
gccuugauguguaggauaggugggaggcuuugaaguguggacgccagucu
gcauggagccgaccuugaaauaccacccuuuaauguuugauguucuaacg
uugacccguaauccggguugcggacagugucugguggguaguuugacugg
ggcggucuccuccuaaagaguaacggaggagcacgaagguuggcuaaucc
uggucggacaucaggagguuagugcaauggcauaagccagcuugacugcg
agcgugacggcgcgagcaggugcgaaagcaggucauagugauccgguggu
ucugaauggaagggccaucgcucaacggauaaaagguacuccggggauaa
caggcugauaccgcccaagaguucauaucgacggcgguguuuggcaccuc
gaugucggcucaucacauccuggggcugaaguaggucccaaggguauggc
uguucgccauuuaaagugguacgcgagcuggguuuagaacgucgugagac
aguucggucccuaucugccgugggcgcuggagaacugaggggggcugcuc
cuaguacgagaggaccggaguggacgcaucacugguguucggguugucau
gccaauggcacugcccgguagcuaaaugcggaagagauaagugcugaaag
caucuaagcacgaaacuugccccgagaugaguucucccugacccuuuaag
gguccugaaggaacguugaagacgacgacguugauaggccggguguguaa
gcgcagcgaugcguugagcuaaccgguacuaaugaaccgugaggcuuaac
c
<<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<....(((.>>>.....<<<<<<<..<<<)))....>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..(((..<<<<......<<<<<<<<<<<<<.<<<...
<<<<<......>>>>>((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>...>>>>........<<<<<<<<.........>>>>>>>>.
.....<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].....
..........<<....>>....]].........<<<<.....>>>>....
..............>>>>>>>>>>>.......<<<<..<<<<<<....>>
>>>>..>>>>...<.<........>.>.<<<<<<....<<<<<<<<<<<<
<..<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>
.....>>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<
<<....<<<<<<......>>>>>>.....>>>...<<<.<<<<<......
..>>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>.
.....>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<
<<<<<<.....<<<...<<<.<<<<...<<<<<<<.....>>>>>.>>..
.>>>>.>>>..>>.>.....>>>>>>.>>>...>>>>>>>.>...<<<<<
<<<<<......>>>>>>>>>>....<<<......>>>...<<<<.<<<..
.<<<(((.....<<<<<<...........<<..<<<<<..<<........
<<<<<......<<<.........>>>.............<<......>>.
..>>>>>....>>..>>>>>.>>...........))).....>>>>>>.>
>>...>>>>>>><<<<<<<<<....>>>>>>>>>....>>>>>>..<<<<
<...<<<......<<<<<<<<....>>>>>>>>...>>>..>>>>>....
....>>>>>><......<<.....<<<<<<.......>>>>>><<<<...
.<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<.<<<
...<<<...<<<....>>>...>>>...>>>..<<<<<........>>>>
>))<<<<<<<<<<<<<...<<<<....<<<<..<<<..<<<<<<<<<<<.
...(((...>>>>>><<..<<<<...<<<<<<<<<<<<.<<.....>>>>
>>>>>>..>>>>...>>>>...>><..<<<<<....>>>>>...>>>>>>
...>>>...>>>>.........>>>>...>>....>>>>>>>>>>>.>>>
..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<
....<<<<<<<<.<<...............<<<<<...............
>>>>><<<<<<.....<<<...<<<<.....>>>>...>>>...>>>>>>
............<<<<<<<<<..<<<.........>>>..<<<<<<<...
..<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<<<
....<...<<<<<<.<<....>>..>>>>>>..>.....>>>>>>>>..>
>>>><<<<<<.......>>>>>>........<...........<<<<<<.
....>>>>>>.....>....>>>>>..>>.>>>>>>>.....>>.>>>>>
>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<<.
(((.[[......<<<<.[[<<<<..<<<<<.<<<.<<<<<.<<<<<<<<<
<<.<<<............<<<<<..<<<......<<<<<<<<....>>>>
>>>>...>>>............>>>>>...>>>.>>>>>>>>>>>...<<
<.<<<<<<.....>>>>>>>>>....>>>>>.>>>..>>>>>..<<<<..
...>>>><<<<<<...........>>>>>>..<<...<<<<<<<...<<<
<<<.......>>>>>>.....<<...(((>>....>>>>>>>...<<<<.
.<<<<....>>>>..>>>>.<<<....>>>.>>))).......<<<<<<<
.((..>>>>..))>>>>>>>.............>>>>..]]>>>>.....
..<<<<...<<<<<<...<<.......>>...>>>>>>....>>>.>...
....<<<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<<<<
.....>>>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>
.>>>>....]]..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<
<...............>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<
))).>>>>>>..>>>>>...>>>>.>>>>>...<<<....<<<<<.....
......>>>>>....>>>.>>>>.......>>>>>>>..<<<<<.....>
>>>><....<<..<<<<<.......>>>>>....<<<<<<<<..<<....
<<<<<....>>>>>...>>...>>>>>.>>>.....>>..>..>>>>>>>
>
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB7n30 Structural basis of early translocation events on the ribosome.
Resolution2.66 Å
Binding residue
(original residue number in PDB)		P4 K5 R6 K8 F9 R10 K11 M12 H13 K14 G15 R16 R18 G23 R40 R44 Q45 A48 R55 K62 W64 R66 F68 D70 K76 L78 A79 X81 M82 G83 K84 G85 K86 K100 A122 K123 P125
Binding residue
(residue number reindexed from 1)		P4 K5 R6 K8 F9 R10 K11 M12 H13 K14 G15 R16 R18 G23 R40 R44 Q45 A48 R55 K62 W64 R66 F68 D70 K76 L78 A79 X81 M82 G83 K84 G85 K86 K100 A122 K123 P125
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0003735 structural constituent of ribosome
GO:0019843 rRNA binding
Biological Process
GO:0000027 ribosomal large subunit assembly
GO:0002181 cytoplasmic translation
GO:0006412 translation
Cellular Component
GO:0005737 cytoplasm
GO:0005840 ribosome
GO:0022625 cytosolic large ribosomal subunit
GO:1990904 ribonucleoprotein complex

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:7n30, PDBe:7n30, PDBj:7n30
PDBsum7n30
PubMed34234344
UniProtP0ADY7|RL16_ECOLI Large ribosomal subunit protein uL16 (Gene Name=rplP)

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