Structure of PDB 8fle Chain LO Binding Site BS01

Receptor Information

>8fle Chain LO (length=95) Species: 9606 (Homo sapiens)

SINSRLQLVMKSGKYVLGYKQTLKMIRQGKAKLVILANNCPALRKSEIEY
YAMLAKTGVHHYSGNNIELGTACGKYYRVCTLAIIDPGDSDIIRS

Ligand information

>8fle Chain L3 (length=3372)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccggggcgcgggacauguggc
guacggaagacccgcuccccggcgccgcgggggcccaaguccuucugauc
gaggcccagcccguggacggugugaggccgguagcggcccccggcgcgcc
gggcccgggucuucccggagucggguugcuugggaaugcagcccaaagcg
ggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauagucaa
caaguaccguaagggaaaguugaaaagaacuuugaagagagaguucaaga
gggcgugaaaccguuaagaggaacgggugggguccgcgcaguccgcccgg
aggauucaacccggcggcggccggccgugucggcggcccggcggaucugg
gaccgucccccgaccggcgaccggccgccgccgggcgcauuuccaccgcg
gcggugcgccgcgaccggcuccgggacggcugggaaggcccggcggggaa
gguggcucgggcccgaguguuacagcccccccggcagcagcacucgccga
aucccggggccgagggaggucgccgcgcucuccccccggggggccgggcc
accccucccacggcgcgaccgcucggggcggacuguccccagugcgcccc
gggcgggucgcgccgucgggcccggggggccacgcgcgcgucccccgaag
agggggacggcggagcgagcgcacggggucggcggcgacgucggcuaccc
acccgacccgucuugaaacacggaccaaggagucuaacacgugcgcgagu
cgggggcucgcacgaaagccgccguggcgcaaugaaggugaaggccgcgg
ccgaggugggaucccgaggccucuccaguccgccgagggcgcaccaccgg
cccgucucgcccgccgcgccggggagguggagcacgagcgcacguguuag
gacccgaaagauggugaacuaugccugggcagggcgaagccagaggaaac
ucugguggagguccguagcgguccugacgugcaaaucggucguccgaccu
ggguauaggggcgaaagacuaaucgaaccaucuaguagcugguucccucc
gaaguuucccucaggauagcuggcgcucuccgcaguuuuauccgguaaag
cgaaugauuagagguaccuauucucaaacuuuaaauggguaagaagcccg
gcucgcuggcguggagccgggcguggaaugcgagugccuagugggccacu
uuugcagaacuggcgcugcgggaugaaccgaacgccggguuaaggcgccc
gaugccgacgcucaucagaccccaaaagguguugguugauauagacagca
ggacgguccgcuaagaccugccgaaucaacuagcccugaaaauggauggc
gcuggagcgucgggcccauacccggccgucgccggcagucgagaguggac
gggagcggcgcgcggacgcuacgccgcgacgaguaggagggccgcugcgg
ugagccuugaagccuagggcgcgggcccggguggagccgccgcaggugca
gaucuuggugguaguagcaaauauucaaacgagaacuuugaaggccgaag
uggagaaggguuccaugugaacagcaguugaacaugggucagucgguccu
gagagaugggcgagcgccguuccgaagggacgggcgauggccuccggccc
ugaucgaaagggagucggguucagauccccgaauccggaguggcggagau
gggcgccgcgaggcguccagugcgguaacgcgaccgaucccggagaagcc
ggcgggagccccggggagaguucucuuuucuuugugaagggcagggcgcc
cuggaauggguucgccccgagagaggggcccgugccuuggaaagcgucgc
gguuccggcggcguccggugagcucucgcuggcccuugaaaauccggggg
agaggguguaaaucucgcgccgggccguacccauauccgcagcaggucuc
caaggugaacagccucuggcauguuggaacaauguagguaagggaagucg
gcaagccggauccguaacuucgggauaaggauuggcucuaagggcugggu
cggucgggcuggggcgcgaagcggggucccguggaucgccccagccggcc
ggcgccuagcagccgacuuagaacuggugcggaccaggggaauccgacug
uuuaauuaaaacaaagcaucgcgaaggcccgcggcggguguugacgcgau
gugauuucugcccagugcucugaaugucaaagugaagaaauucaaugaag
cgcggguaaacggcaagugaacgagauucccacugucccuaccuacuauc
cagcgaaaccacagccaaggcgggcuuggcggaaucagcggggaaagaag
acccuguugagcuugacucuagucuggcacggugaaguuuuuucacugac
ccggugaggcggggggcuucuggcgccaagcgcccggccgcgcgccggcc
gggcgcgacccgcuccggggacagugccagguggggaguuugacuguaca
ccugucaaacgguaacgcagguguccuaaggcgagcucagggaggacaga
aaccucccguggagcagaagggcaaaagcucgcuugaucuugauuuucag
uacgaauacagaccgugaaagcggggccucacgauccuucugaccuuuug
gguuuuaagcaggaggugucagaaaaguuaccacagggaugaugucggcu
cuuccuaucauugugaagcagaauucaccaagcguuggauuguucaccca
cuaauagggaacgugagcuggguuuagaccgucgugagacagguuaguuu
uacccuacugaugauguguuguugccaugguaauccugcucaguacgaga
ggaaccgcagguucagacauuugguguaugugcuuggcugaggagccaau
ggggcgaagcuaccaucugugggauuaugacugaacgccucuaagucaga
aucccgcccaggcggaacgauagcgccgcggagccucgguuggccucgga
uagccgguccccgggguccggugcggagugcccuucguccugggaaacgg
ggcgcggccggagaggcggccgcccccucgcccgucacgcaccgcacguu
cguggggaaccuggcgcuaaaccauucguagacgaccugcuucugggucg
ggguuucguacguagcagagcagcucccucgcugcgaucuauugaaaguc
agcccucgacacaaggguuugu

.........................<<<<<<.....<......>......
>>>>>.>.........<<....>>..<<<<<<.....<<.....>>....
>>>>>>..<<<..<<<<<...<<<<<<<<<..>>>>>.>>>>.....>>>
>>..<<<<<<<<<<...<<<<<<<<.<<<<<<<<<<....<<<<......
>>>>.....((......<<<.....))>>>...>.>>>>>>>.>>>>>>>
>>>..>>>>>>>>>>.......<<<<<<<........>>>>>>>.....<
<<<<..((((((>>>>>..>>>............................
......<<....>>...............<<<......>>>.........
.......................<<<<<<<<..<<<.<<...<<<<<<<<
.<<<.....<<<<<<<<<<<<<<<<<<.<<<<<.<<...<<<<<.<<...
>>>>>>>>>>>>>>>>>>...>>>>>>>>>>>>>>......>>>.>>>.>
>>>>...<<<<<<<<<<<<<<<<<<<<<<<...<<..<<<.<<.<<<<..
....<<<<<<..>>>>>>.......>>>>>>.>>>........>>>>>>.
.>>>>>>>>>>>............<<<<<<.<<<<<..>>>>>.<<<<<<
.<<<<.<<<<<<<<<<..<<<<<<<<<<<<<...........>.>>>>>>
>>>>>>...>>>>>>>.>>>...>>>>>>>>.>>..<<<<<<<<<.....
..>>>>>>>.>>....>>>>>>.....>>>.>>>>>...>>>>>...>>>
>>>>>..<<<<........>>>><<<<<<....<<<<<<<<<<<<<..<<
<...<<<<........>>>>.....>>>...<<....>>...<<<<..>>
>>..<<<<<...<<<..<<<..((((.((((>>>..>>>....>>>>>.<
<<<<<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>
..<<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>
>>>>>>.....>>>...<<<..<<.<<........>>>>.>>>....>>>
>>>>>>...<<.......>>......>>>>>>...>..>.>>..<<<<<<
....<.<<<....>>>.><<.<<<<<<<...<<<.<<<<<<<<<...<<<
....<<<....<<<..>>>....>>>..>>.>...>>>>>>>...<<<<<
<<<<.<......>>>>>>>>>>....>>>>>>>>>>>>..>>..<<<<.<
<<<..>>>>.>>>>....<<<......>>>...<<<<<<<...<<<(((.
......<<<<<.....<<........<<<.<<<<<<<<<<.......<<<
<<.<<<.>>>......>>>>>...>>>>>>>>>>>>>..........)))
.>>...>>>>>..>>>...>>>>>>><<<<<<.<<<..........))))
.)))).................>>>>>>>>>....>>>>>>.<.<<<<<<
<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>..
...>>>>>><<.....<<.....<<<<<<.......>>>>>>.<<<....
.<<<<<.<<...<<<<<....<<.....>>..>>>>>....((<<<<<<.
..<<....<<<....>>>.............<<<<...........>>>>
...>>....>>>..<<<<<........>>>>>))<<<<....<<<<<..<
<<<<<<<....>>>>>>>>..<<<....>>>...<..<<<<<<<...<<<
<<<<<<<<<.<<<<<<<<<<..[[[..>>>>>>....<<<<<.<<<<.<<
<<.....<<<.....>>>.....>>>>>>>>..>>>>>.....<<<<<<<
.......>>>>>>>>>>>...>>>>>>>>>>>>.........>>>>>>>.
.>..........>>.>>>.>>>>.>>>..>>.....<<......>>>>>>
>.<<<<.....>>>>..>>>....<<<<.....<<<<<<<.<<.......
........<<<<<....<<..>>.....>>>>><<.<<<<<<<<<<<.<<
<<<<<<.<<<<<<<<<....<<<<<..>>>>>....>>>>>>>>>>>>>>
>>>......>>>>>..>>>>>>.>>............<<<<<<<<<..<<
<.........>>>..<<<<<<<....<<<<.....>>>>......>>>>>
>>.......<<<..<<<<<<.<..<<<.....<<<<....>>>>.....>
>>>>>>>..>>>>>.......>>.>>>>>>>.....>>.>>>>>>>..>>
>>>>....>>...<<<<<<.....>>>>>>...<<<<<..<<<.......
.<<<<<.<<<<<<..<<<<<.<<<<<<<<<.<<<<<.....>>>>>...<
<<<<.....<<<<................<<<<<<<<<<.......>>>>
>>>>>>..>>>>..>>>>>....>>>>>>>>>..>>>>>...<<.>>..<
<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<.....
..>>>>>>.....<<.[.(((>>....>>>>>>>...<<<.<<<<<....
...>>>>.>>>>.<<<....>>>.>>)))].....<<<<<<<..<<<..)
))))).>>>>>>>>>>.........>>>>..>>>>>>>..((...<<<<.
<<<<<<..<.<.<<<<<......>>>>>...>.>.<<<.<...))>.>>>
.....>>>>>..>...>>>>......<<<<<<<....>>>.>>>>.....
...>>>.>>>>>.....<<<<<<.<..<<<..<.<<<<<...........
......>>>>>.><<<<....<<<<....<<.<<<<<<.]]]..>>>>..
>>.>>...>>>>...>>>><<<<<...<<<<<...........>>>>>..
.>>>>>.>>>...>.>>>>>>.<<<<<<.<<..<<<<....<<<......
..<<<<<.<<<<>>>>.>>>>>.......>>>...<<<..<<<<....<<
<<.<<<<<<<......>>>>>>>>>>>...>>>>..>>>...........
...>>>>..>>>>>>>>.<<<<<.<.............>....<<.<<<<
<<<.<<.....<<<<....<<<<<......>>>>>...>>>>....>>..
..>>>.>>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fle Principles of human pre-60S biogenesis
• Resolution: 2.48 Å
• Binding residue (original residue number in PDB): L29 G30 Y31 K32 Q33 A54 R56 S58 Y88 Y89 R90 C92
• Binding residue (residue number reindexed from 1): L17 G18 Y19 K20 Q21 A42 R44 S46 Y76 Y77 R78 C80

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0031640 killing of cells of another organism
• GO:0050829 defense response to Gram-negative bacterium
• GO:0061844 antimicrobial humoral immune response mediated by antimicrobial peptide

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0005925 focal adhesion
• GO:0014069 postsynaptic density
• GO:0016020 membrane
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0045202 synapse
• GO:0070062 extracellular exosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fle, PDBe:8fle, PDBj:8fle
• PDBsum: 8fle
• PubMed: 37410842
• UniProt: P62888|RL30_HUMAN Large ribosomal subunit protein eL30 (Gene Name=RPL30)