Structure of PDB 8p5d Chain LN0 Binding Site BS01

Receptor Information

>8p5d Chain LN0 (length=203) Species: 1358809 (Spraguea lophii 42_110)

GYLQYLKEIRRDKQSSVMRYLLRVRCWEYRQNTAIHRASGPTYLDKARML
GYRNIPGMCIFRARVRRGARPKKVLKGNTHGKPSKSGVYQRKPAKRLQAF
AEIRVGRKCSNMRVFNSYWVGQDRVYKYFEVIMVDPYCDRIKNDKKINWL
CKPVMKHREARGLTNGTKTSRGLGKGIRYNQTKGGSKRAAWRNRNTQSLR
RYR

Ligand information

>8p5d Chain L50 (length=2499)

auaccacacacaagggaucguuuggcuccugggacgaggaagggcgcagc
agagagcgauaugugugggagcagcagcaaugaccacacguccccgaaua
cagcauagugcuggaguacuccuuggaauuaagcauaugaguaaaggaag
gaaaagaaacuaacgaggauucccguaguagcggcgagugaacagggaac
aguccaacaguguaauucucaaaugagaaguugucaaaaggaagaagaug
ugaaucagaaagaaaagcugaacgauacagggugauagucccguaguguc
uucuuacauacggugaguagcugugcucgguaaugcacagugaagaggug
gcagugugcaucuaagacuaaauacaacaggagaccgauagcaaagaagu
agguugaccgaaaacagugaaauuguguguggacccgaauggaucgggcc
cgucuugaaacacggaccaaggagugcacauacacugcaaguugagcaga
ggaagucugugaggcguagugaagacggaaagcagugugagugcgacccg
auagacuugugaacuauaccuuguugcugugaaggcuggcgaaagccagc
uggaggcggcaagccguauugaucugcaaaucauuggcguaagcagggua
uaggggcgaaagaccaaucgaacagucuaguugcugguucccuccgaaau
gucuaucaggacagcgcgcacgcagagagaagcaagguagagcaugggcc
gguauccuuaaggagacggacgaacugcgaaugugcuuuucuucuaugca
agcguagugggcgaguucugguaagcaggacuggcgaagaggaaugaacc
uggcacugugcuaaggaacccaaugucuggacagagaccgaaagagguag
gugaauaaaauucaucugcucugaaaauugauggcgcuuaccagacaucc
gaugcacaguuagcagguaggagggcgugugucuacuggcgaagguaccg
agugaucggugguggagguagaugcagagcagaucuuggugguaguagcg
auaauuugucuucagcggcaaagacugaggaggagaaggguuucuucuca
uaagaagagugagccgggucuaagcgaguguguagaagcaugagcgaagg
agaaacagguugauauuccugugccugugcugucguacggcaacguguau
uagcuugucgacacgaacaucuagacaaagcagaaacaccuuuucuguuc
aaggggaaaaagaccacugagacggaucaucuggcgacgugguuuuauau
cugugaccaggccugagccugguuuguugaggauggucguccaugaaaag
acaacuauauggcagcacaguccguaccaaccguaucaggacuccaaggu
gaagagccucuaguggauguuuuacacauguaagggaauucggcaaaaug
gaugcguaacuucgggagaaguauugguucaauugugaacucauugugac
aaggggaaucugacuguuuaguaaaaacauagcuucaugaagagagauga
agugaguucugcccggugcucggucgugacacggaggaaaugccagaaag
cacggguuaacggcgggaguaacuaugacucucuuaagguagccaaacgc
cucgucauuuaaauggugacgcgcaugaauggaaugacgagauucccacu
gucccuacauguagacuagcgaacccauuuccaggggaacgggccuggac
ggccagcggggaaagaagacccuguugagcuugacucuaguguggccaag
uugugacgauugaggcgagcgccaugaucaucacgacacacuuguuuaag
gacaaaggccagauggggaguuuggcuggggcgguacgaccacagaauca
gaacguggucgaccgaagguacucacagcgagacgagaacacucguguag
agcauaaggacuaaagagugccugagugugagcacuacugcuugcaugag
gggaaacccgggccuagcgauccuacgcauacgagacacuuguggcgugg
gugucagaaaaguuaccacagggauaacuggcuuguggccgccgagcgua
gaaagcgacgcggcuuuuugauucuucgaugucggcucuuccuagcaugg
ccaugcagccguggcgaaguguuggauuguucacccacuaacagggaacg
ugagcuggguuuagaccgucgugagacagguuaguuuuauccuacugucc
aaacaguugaagggaguguggauuaguacgaaaggaaacccacacgugac
cucuggugcagcgggugugcggaagugcaacugccaugcuacgucuccuc
aagcauagcuggaagccucuaagcuagaaaugaguccugacucuggaaga
cggccgggaagacgacccguaguuaacagcgguguuguacgauaugagcu
uucuauuguaguagcgaguauuugagacacugguuguuaccaccgucgu

....<<<<<<<<<......((..((<<<<<<...<......<<<<<<.((
.....>>>....<<<<<<..<..))..>.....>>>>>>...>>>.....
<<<<<...>>>>>>....<<<<<<.....<......>......>>>>>.>
.........<<....>>..<<<<<......<<.....>>.....>>>>>.
.<<<...........<<<<<<..>>>>>>............<<<<<<<<<
<...<<<<.........>>>>((......<<<.....))>>>...>>>>>
>>>>>..............<<<<<<<........>>>>>>>....<<<<<
..<<<<<.>>>>>..>>>.........>>>>>>.))..............
.<<....>>....)).......>>>>>>>>>..<<<<<.....>>>>>.<
<<<........>>>><<<<<<....<<<<.<<<<<<<<..<<<..<<<<<
.....>>>>>..>>>...<<....>>.....>>>>>>>>.>>>>..<<.<
..<<<<<.<<.....<<<<<<<<<<<<<<....<<<<<<<....>>>>>>
>.....>>>>>.<<<..<<.<<........>>>>.>>>....>>>>>>>>
>...<<.......>>......>>>>>>...>..>.>>..<<<<<<....<
<<<<....>>>>><<<<<<..<<<<<.<<<<<<<.....<<<......<<
<...<<<<..>>>>..>>>.....>>.>.....>>>>>..>>>>>..>>.
.>>>>.>>..<<.<<<<<<<.....>>>>>>>.>>....<<<......>>
>...<<<<<<<...<<<.((...<<<<<<<.....<......<<.<<<<<
<<<<<<...>>>>>>>>>>>>>..........))..>...>>>>>>>>>>
...>>>>>>>.........>>>>>><.<<<<<<<<<<......<.<<<<<
<....>>>>>>.>...>>>>>>>>>>..>.....>>>>>><<.....<<.
....<<<<<<......>>>>>>.<<<.....<<<<..<<...<<<<<<..
..>>>>>>....((<<<<<<...<<..<<<<......>>>>..>>....>
>...<<<<<........>>>>>))<<<<<<<<<<..<<<....>>>....
<<<.<<<<<...<<<<.<<<<..<<<<<<<<<<<<...[[..>>>>>><<
..<<<..<.<<<<<<<<.....<<<<...>>>>.....>>>>>>>>.>.>
>>..>><<<<<<....>>>>>>>>>>>>...>>>>.>>>>.........>
>>>>>>>...>>>>>>>>>>>>>>..>>..<<......>>.>>>>.<<<<
.....>>>>..>>>....<<.....<<<<<<<.<<.........<.....
<<<<<.....<..>......>>>>><<<<<<....>>>>>>.........
...<<<<<<<<<..<<<.........>>>..<<<<<<<........>>>>
>>>..<<<..<<<..<<<<<<<.<.<<.....<<........>>.....>
>>>>>>>..>>>>><<<<<<.......>>>>>>........<........
...<<<<<<.....>>>>>>.....>.....>>.>..>>.>>>>>>>..>
..>>.>>>>>>>..>>..>>....>>....<<<<<........>>>>>..
[[.<<<..<<<.[[......<<<<.[[<<<<..<<<<<.<<.<<<<<..<
<<<<<<.<<<<..<<<<..>>>>..>>>>.>>>>>>>...<<<....>>>
......>>>>>.>>..>>>>>...<.<.....>.>..<<<<<<.......
....>>>>>>..<<...<<<<<<<...<<<<<.........>>>>>....
<<..[.(((.>>...>>>>>>>...<<<<<<<<......>>>>>>>>..<
<<....>>>.>>)))]......<<<<<<.<<<...>>>>>.>>>>>>>>>
..........>>>>..]]>>>>.......<<.<.<.<<<<<<...<<...
....>>...>>>>>>...>>..>>.......<<<<<.<<<<<<..<<<.<
<<<<......>>>>>...>>>.<<<.........>>>.....>>>>>..>
...>>>.>>....<<<<<<<....>>>.>>>>....]]..>>>.>>>.]]
...<<<....<<<<.<<<<<<..................>>>>>><.<<<
...<<<<...<<<<.<<<<.]]...>>>>.>>>>...>>>>.>>>.><<<
<....<<<<<...........>>>>>....>>>>>>>>....>>>...<<
<<<.<<<<.......>>>>....<<<<<<<<<<<<<....<<<<<..<<<
..<<<...>>>.>>>..>>>>>...>>>>>>.>>>>>>>...>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8p5d Spraguea lophii ribosome
• Resolution: 10.8 Å
• Binding residue (original residue number in PDB): L4 Q5 K8 R12 R20 R24 W28 T34 A35 R38 K47 R49 M50 R54 R68 G69 A70 R71 K74 K77 N79 K83 P84 S85 K86 Y90 Q91 K93 P94 A95 K96 R97 R105 R108 K109 S111 N112 W120 D124 R125 Y138 D140 W150 K153 P154 R162 K169 T170 S171 R172 K176 G177 I178 R179 N181 Q182 K184 G185 S187 R189 W192 R193 R195 R201 R202 Y203 R204
• Binding residue (residue number reindexed from 1): L3 Q4 K7 R11 R19 R23 W27 T33 A34 R37 K46 R48 M49 R53 R67 G68 A69 R70 K73 K76 N78 K82 P83 S84 K85 Y89 Q90 K92 P93 A94 K95 R96 R104 R107 K108 S110 N111 W119 D123 R124 Y137 D139 W149 K152 P153 R161 K168 T169 S170 R171 K175 G176 I177 R178 N180 Q181 K183 G184 S186 R188 W191 R192 R194 R200 R201 Y202 R203

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8p5d, PDBe:8p5d, PDBj:8p5d
• PDBsum: 8p5d
• PubMed: 37709902
• UniProt: S7W7A2