Structure of PDB 8bsj Chain LF Binding Site BS01

Receptor Information
>8bsj Chain LF (length=289) Species: 5741 (Giardia intestinalis) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
WVKVQKTRSYFKRFQVQFRRRREGKTDYQARKALIIQDKNKYATPRYRFV
VRRTNKDIICQVTAAELTKDRVVCAAYSHELPRYGIPVGLTNYAAAYATG
LLCGRRLLEKLSVGIKENALAQLYPGLDKATGEEYHPDENENEEVHAFKA
FLDIGLARASTGARVFAAMKGAVDAGLNIPHSMKRFPGYSKDGFDSSALR
DRIMGKHVGDYMAELKAEDEETYRKRFSKYIAAKVEPLRLEAMYTKAHAA
IRANPQAVKAKFEIKKPVRQARLTLKERKERIDAKKLEA
Ligand information
>8bsj Chain LE (length=116) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
guucggccauccuacggcggaaacuccuuaacucguuccgaauuaggaag
agaagcgccgucaggccccgccaguacugcgaucggagacgucgugggaa
cacggggugccgaacu
<<<<<<<....<<<<<<<<.....<<<<<...............>>>..>
>....>>>>>>.>><<<<<<......<<<<<<.<<....>>>>>>>>...
..>>>>>>>>>>>>>.
Receptor-Ligand Complex Structure
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PDB8bsj Insights into translocation mechanism and ribosome evolution from cryo-EM structures of translocation intermediates of Giardia intestinalis.
Resolution6.49 Å
Binding residue
(original residue number in PDB)
R10 F13 K14 F16 V18 F20 R21 R22 R24 K27 T28 Y30 R33 R50 R54 T56 N57 K58 Q63 L69 T70 K71 D72 R73 V74 Y79 N94 Y95 S162 T163 A165 R166 H209 V210 Y213 R228 F229 S230 K231 Y232 K261 F264 K267 K268 R271 Q272 A273 R274 L275 L277 R280 R283 I284
Binding residue
(residue number reindexed from 1)
R8 F11 K12 F14 V16 F18 R19 R20 R22 K25 T26 Y28 R31 R48 R52 T54 N55 K56 Q61 L67 T68 K69 D70 R71 V72 Y77 N92 Y93 S160 T161 A163 R164 H207 V208 Y211 R226 F227 S228 K229 Y230 K259 F262 K265 K266 R269 Q270 A271 R272 L273 L275 R278 R281 I282
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003735 structural constituent of ribosome
GO:0008097 5S rRNA binding
Biological Process
GO:0000027 ribosomal large subunit assembly
GO:0006412 translation
Cellular Component
GO:0005737 cytoplasm
GO:0005840 ribosome
GO:0022625 cytosolic large ribosomal subunit
GO:1990904 ribonucleoprotein complex

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Biological Process

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Cellular Component
External links
PDB RCSB:8bsj, PDBe:8bsj, PDBj:8bsj
PDBsum8bsj
PubMed36912103
UniProtE2RU47

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