Structure of PDB 8fl2 Chain LC Binding Site BS01

Receptor Information

>8fl2 Chain LC (length=176) Species: 9606 (Homo sapiens)

MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQ
LKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDL
TTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKI
KFPLPHRVLRRQHKPRFTTKRPNTFF

Ligand information

>8fl2 Chain L3 (length=3495)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccggggcgcgggacauguggc
guacggaagacccgcuccccggcgccgcgggggcccaaguccuucugauc
gaggcccagcccguggacggugugaggccgguagcggcccccggcgcgcc
gggcccgggucuucccggagucggguugcuugggaaugcagcccaaagcg
ggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauagucaa
caaguaccguaagggaaaguugaaaagaacuuugaagagagaguucaaga
gggcgugaaaccguuaagagguaaacgggugggguccgcgcaguccgccc
ggaggauucaacccggcggcggccggccgugucggcggcccggcggaucu
gggaccgucccccgaccggcgaccggccgccgccgggcgcauuuccaccg
cggcggugcgccgcgaccggcuccgggacggcugggaaggcccggcgggg
aagguggcucgggcccgaguguuacagcccccccggcagcagcacucgcc
gaaucccggggccgagggagucgccgcgcucuccccccggggggccgggc
caccccucccacggcgcgcgccgucgggcccggggggccacgcgcgcguc
ccccgaagagggggacggcggagcgagcgcacggggucggcggcgacguc
ggcuacccacccgacccgucuugaaacacggaccaaggagucuaacacgu
gcgcgagucgggggcucgcacgaaagccgccguggcgcaaugaaggugaa
ggccgcggccgaggugggaucccgaggcccgccgagggcgcaccaccggc
ccgucucgcccgccgcgccggggagguggagcacgagcgcacguguuagg
acccgaaagauggugaacuaugccugggcagggcgaagccagaggaaacu
cugguggagguccguagcgguccugacgugcaaaucggucguccgaccug
gguauaggggcgaaagacuaaucgaaccaucuaguagcugguucccuccg
aaguuucccucaggauagcuggcgcucuccgcaguuuuauccgguaaagc
gaaugauuagaggucuuggggccggaucucaaccuauucucggguaagaa
gcccggcucgcuggcguggagccgggcguggaaugcgagugccuaguggg
ccacuuuugguaagcagaacuggcgcugcgggaugaaccgaacgccgggu
uaaggcgcccgaugccgacgcucaucagaccccagaaaagguguugguug
auauagacagcaggacgguggccauggaagucggaauccgcuaaggagug
uguaacaacucaccugccgaaucaacuagcccugaaaauggauggcgcug
gagcgucgggcccauacccggccgucgccggcagugacgcuacgccgcga
cgaguaggagggccgcugcggugagccuugaagccuagggcgcgggcccg
gguggagccgccgcaggugcagaucuuggugguaguagcaaauauucaaa
cgagaacuuugaaggccgaaguggagaaggguuccaugugaacagcaguu
gaacaugggucagucgguccugagagaugggcgagcgccguuccgaaggg
acgggcgauggccuccggcccucgaucgaaagggagucggguucagaucc
ccgaauccggaguggcggagaugggcgccgcgaggcguccagugcgguaa
cgcgaccgaucccggagaagccggcgggagccccggggagaguucucuuu
ucuuugugaagggcagggcgcccuggaauggguucgccccgagagagggg
cccgugccuuggaaagcgucgcgguuccggcggcguccggugagcucucg
cuggcccuugaaaauccgggggagaggguguaaaucucgcgccgggccgu
acccauauccgcagcaggucuccaaggugaacagccucuggcauguugga
acaauguagguaagggaagucggcaagccggauccguaacuucgggauaa
ggauuggcucuaagggcugggucggucgggcuggggcgcgaagcgggguc
ccguggaucgccccagccggccggcgccuagcagccgacuuagaacuggu
gcggaccaggggaauccgacuguuuaauuaaaacaaagcaucgcgaaggc
ccgggguguugacgcgaugugauuucugcccagugcucugaaugucaaag
ugaagaaauucaaugaagcgcggguaaacggcgggaguaacuaugacucu
cuuaagguagccaaaugccucgucaugugacgcgcaugaauggaugaacg
agauucccacugucccuaccuacuauccagcgaaaccacagccaagggaa
cgggcuuggcggaaucagcggggaaagaagacccuguugagcuugacucu
agucuggcacggugaagagacaugagagguguagaauaagugggaggcgg
ccgccggugaaauaccacuacucugaucguuuuuucacugacccggugag
gcggggggcuucuggcgccaagcgcccggccgcgcgccggccgggcgcga
cccgcuccggggacagugccagguggggaguuugguacaccugucaaacg
guaacgcagguguccuaaggcgagcucagggaggacagaaaccucccgug
gagcagaagggcaaaagcucgcuugaucuugauuuuguacgaauacagac
cgugaaagcggggccucacccuucugaccuuuuggguuuuaagcaggagg
aaguuaccacagggauaacuggcuuguggcggccaagcgagcgacgucgc
uuuuugauccuucgaugucggcucuuccuaucauugugaagcagaauuca
ccaagcguuggauuguucacccacuaauagggaacgugagcuggguuuag
accgucgugagacagguuaguuuuacccuacugaugauguguuguugcca
ugguaauccugcucaguacgagaggaaccgcagguucagacauuuggugu
augugcuuggcugaggagccaauggggcgaagcuaccaucugugggauua
ugacugaacgccucuaagucagaaucccgcccaggcggaacgauagcgcc
gcggagccucgguuggccucggauagccgguccccgggguccggugcgga
gugcccuucguccugggaaacggggcgcggccggagaggcggccgccccc
ucgcccgucacgcaccgcacguucguggggaaccuggcgcuaaaccauuc
guagacgaccugcuucugggucgggguuucguacguagcagagcagcucc
cucgcugcgaucuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<.....<<....>>......
>>>>>.>.........<<....>>..<<<<<<.....<<.....>>....
>>>>>>..<<<..<<<<<...<<<<<<<<<..>>>>>.>>>>.....>>>
>>..<<<<<<<<<<...<<<<<<<<.<<<<<<<<<<....<<<<......
>>>>.....((......<<<.....))>>>...>.>>>>>>>.>>>>>>>
>>>..>>>>>>>>>>.......<<<<<<<........>>>>>>>.....<
<<<<..((((((>>>>>..>>>............................
......<<....>>...............<<<......>>>.........
.........................<<<<<<<<..<<<.<<...<<<<<<
<<.<<<.....<<<<<<<<<<<<<<<<<<.<<<<<.<<...<<<<<.<<<
.>>>>>>>>>>>>>>>>>>>...>>>>>>>>>>>>>>......>>>.>>>
.>>>>>...<<<<<<<<<<<<<<<<<<<<<<<...<<..<<<.<<.<<<<
......<<<<<<..>>>>>>.......>>>>>>.>>>........>>>>>
>..>>>>>>>>>>>...........<<<<<<.<<<<<..>>>>>.<<<<<
<.<<<<.<<<<<<<<<..>>>>>>.>>>...>>>>>>>>.>>..<<<<<<
<<<.......>>>>>>>.>>....>>>>>>.....>>>.>>>>>...>>>
>>...>>>>>>>>..<<<<........>>>><<<<<<....<<<<<<<<<
<<<<..<<<...<<<<........>>>>.....>>>...<<....>>...
<<<<..>>>>..<<<<<...<<<..<<<..>>>..>>>....>>>>>.<<
<<<<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>.
.<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>
>>>>>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>
>>>>>...<<.......>>......>>>>>>......>.>>..<<<<<<.
.....<<<....>>>..<<<<<<<<<<...<<<.<<<<<<<<........
....<<....<<<..<<<<<<.<.>.>>>>>>>>>..>>..>>>>>>...
<<<<<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>>.>>>..<
<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<
...<<<(((.......<<<<<.....<<..........<<<.<<<<<<<<
<<.......<<<<<.<<<...<<.........>>...>>>.........<
<......>>...>>>>>...>>>>>>>>>>>>>..........))).>>.
..>>>>>..>>>...>>>>>>><<<<<<.<<<...........>>>>>>>
>>....>>>>>>.<.<<<<<<<<.<<......<<<<.<<<<....>>>>>
>>>...>>>>>>>>>>..>.....>>>>>><<.....<<.....<<<<<<
.......>>>>>>.<<<.....<<<<<.<<...<<<<<....<<.....>
>..>>>>>....((<<<<<<...<<....<<<....>>>...........
..<<<<...........>>>>....>>....>>>..<<<<<........>
>>>>))<<<<....<<<<<..<<<<<<<<....>>>>>>>>..<<<....
>>>...<..<<<<<<<...<<<<<<<<<<<<.<<<<<<<<<<..[[[..>
>>>>>....<<<<<.<<<<.<<<<.....<<<.....>>>.....>>>>>
>>>..>>>>>.....<<<<<<<.......>>>>>>>>>>>...>>>>>>>
>>>>>.........>>>>>>>..>..........>>.>>>.>>>>.>>>.
.>>.....<<......>>>>>>>.<<<<.....>>>>..>>>....<<<<
.....<<<<<<<.<<...............<<<<<.....<..>......
>>>>><<.<<<<<<<<<<<.<<<<<<<<.<<<<<<<<<....<<<<<..>
>>>>....>>>>>>>>>>>>>>>>>......>>>>>..>>>>>>.>>...
.........<<<<<<<<<..<<<.........>>>..<<<<<<<....<<
<<.>>>>......>>>>>>>.......<<<..<<<<<<....<<<.....
<<<<....>>>>.....>>>.>>>>..>>>>><<<<<<.......>>>>>
>.............<<<<..<<<<<<>>>>>>.>>>>...........>>
.>>>>>>>.....>>.>>>>>>>..>>>>>>....>>....<<<<<....
....>>>>>....<<<<<..<<<........<<<<<.<<<<<<..<<<<<
.<<<<<<<<<.<<<<<<<<<<<<.<<<<..........<<<<<..<<<.>
>>............>>>>>.>>>>.>>.>>>>>>>>>>...<<<<<....
.<<<<................<<<<<<<<<<.......>>>>>>>>>>..
>>>>..>>>>>....>>>>>>>>>..>>>>>..<<.<<<<<<<.......
......>>>>>>>>>...<<<<<<<...<<<<<<.......>>>>>>...
..<<...(((>>....>>>>>>>...<<<..<<<<.[[..>>>>..>>>.
<<<....>>>...))).]]<<<<<<<..<<<..)))))).>>>>>>>>>>
.>>>>..>>>>>>>.............<<<<<<...<<..>>...>>>>>
>.................<<<<.<<<<<<..<.<.<<<<<......>>>>
>.>...>.<<<.........>>>.....>>>>>..>...>>>>......<
<<<<<<....>>>.>>>>........>>>.>>>>>.....<<<<<<.<..
<<<..<.<<<<<.................>>>>>.><<<<....<<<<..
..<<.<<<<<<<]]].>>>>>..>>.>>...>>>>...>>>><<<<<...
<<<<<...........>>>>>...>>>>>.>>>...>.>>>>>>.<<<<<
<.<<..<<<<....<<<........<<<<<.<<<<>>>>.>>>>>.....
..>>>...<<<..<<<<....<<<<.<<<<<<<......>>>>>>>>>>>
...>>>>..>>>..............>>>>..>>>>>>>>.<<<<<.<..
...........>....<<.<<<<<<<.<<.....<<<<.<..<<<<<...
...>>>>>..>>>>>....>>....>>>.>>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fl2 Principles of human pre-60 S biogenesis.
• Resolution: 2.67 Å
• Binding residue (original residue number in PDB): S4 G5 Y63 Q66 F68 K70 P72 L73 S86 R87 S88 H91 N92 M93 Y94 R95 R98 R111 G114 A115 R116 H117 R118 R120 R139 H146 D147 S148 H156 R157 V158 R160 R161 H163 P165 R166 T169 K170 P172 N173 T174 F175 F176
• Binding residue (residue number reindexed from 1): S4 G5 Y63 Q66 F68 K70 P72 L73 S86 R87 S88 H91 N92 M93 Y94 R95 R98 R111 G114 A115 R116 H117 R118 R120 R139 H146 D147 S148 H156 R157 V158 R160 R161 H163 P165 R166 T169 K170 P172 N173 T174 F175 F176

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0016020 membrane
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fl2, PDBe:8fl2, PDBj:8fl2
• PDBsum: 8fl2
• PubMed: 37410842
• UniProt: Q02543|RL18A_HUMAN Large ribosomal subunit protein eL20 (Gene Name=RPL18A)