Structure of PDB 8fkt Chain LC Binding Site BS01

Receptor Information

>8fkt Chain LC (length=176) Species: 9606 (Homo sapiens)

MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQ
LKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDL
TTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKI
KFPLPHRVLRRQHKPRFTTKRPNTFF

Ligand information

>8fkt Chain L3 (length=2125)

gcgaccucagaucagacguggcgacccgcugaaagcauauuagucagcgg
aggagaagaaacuaaccaggauucccucaguaacggcgagugaacaggga
agagcccagcgccgaauccccgccccgggggcgcgggacauguggcguac
ggaagacccgcuccccggcgccgcucguggggggcccaaguccuucugau
cgaggcccagcccguggacggugugaggccgguagcggcccccggcgcgc
cgggcccgggucuucccggagucggguugcuugggaaugcagcccaaagc
gggugguccaucuaaggcuaaauaccggcacgagaccgauagucaacaag
uaccguaagggaaaguugaaaagaacuuugaagagagaguucaagagggc
gugaaaccguuaagaggaacgggugggguccgcgcaguccgcccggagga
uucaacccggcggcgccgggcgcauuuccaccgcggcggugcgccgcgac
cggcuccgggacggcugggaaggcccggcggggaagguggcucgggcccg
aguguuacagcccccccggcagcagcacucgccgaaucccggggccgagg
ggucggcggcgacgucggcuacccacccgacccgucuugaaacacggacc
aaggagucuaacacgugcgcgagucgggggcucgcacgaaagccgccgug
gcgcaaggugggaucccgaggcccaguccgccgagggcgcaccaccggcc
gcacgagcgcacguguuaggacccgaaagauggugaaaccaucuaguagc
ugguucccuccgaaguuucccggauagcuggcgcucuccgcaguuuuauc
cgguggguaagaagccggcguggaaugcgagugccuagugggccacuuuu
gguaagcagaacuggcgcugcgggaugaaccgaacgccggguuaaggcgc
ccgaugccgacgcucaucagaccccagaaaagguguugguugauauagac
agcaggacgguggccauggaagucggaauccgcuaaggaguguguaacaa
cucaccugccgaaucaacuagcccugaaaauggauggcgcuggagcgucg
ggcccauacccggccgucgccggcagucgagaguggacggccgcggacgc
uacgccgcgacgaguaggagggccgcugcggugagccuugaagccuaggg
cgcgggcccggguggagccgccgcaggugcagaucuuggugguaguagca
ggggcgauggccuccggcccucgcggagaugggcgccgcgaggcguccag
ugaaucucgcauguagguaagggaaguacugucccuaccuacuauccagc
gaaaccacagccaagggaacgggcuuggcggaaucagcggggagaagacc
cuguugagcuugacucuagucuggcacggugaagagacaugagaggugua
gaauaagugggaggcggccgccggugaaauaccacuacucugaucguuuu
uuccagugccagguggggaguuauccuucugaccuuuuggguuuuaagca
ggaggugucagaaaaguuaccacagggauaacuggcuuguggcggccaag
cgcgacgucgcuuuuugaugaugucggcucuuccuaucauugugaagcag
aauucaccaagcguuggauuguucacccacuaauagggaacgugagcugg
ggucgugagaccccuacugaugguuguugccaugguaauccugcucagua
cgagaggaaccgcagguucagacauuugguggugcuuggcugaggagcca
auggggcgaagcuaccaucugugggauuaugacugaacgccucuaaguca
gaaucccgcccaggcggaacgauaagcgccgcggagccucgguuggccuc
ggauagccgguccccgggguccggugcggagugcccuucguccugggaaa
cggggcgcggccggagaggcggccgcccccucgcccgucacgcaccgcac
guucguggggaaccuggcgcuaaaccauucguagacgaccugcuucuggg
ucgggguuucguacguagcagagcagcucccucgcugcgaucuauugaaa
gucagcccucgacacaaggguuugu

........................<<<<<<...............>>>>>
.>.........<<....>>..<<<<<<.....<<.....>>....>>>>>
>..<<<..<.<<<...<<<<<<<<<<.>>>>>>.>>>>.....>>>.>..
<<<<<<<<<<...<<<<<<<<.<<.....<<<<<<<<....<<<<.....
.>>>>.....((......<<<.....))>>>...>.>>>>>>>.>>>>>>
>>>>..>>>>>>>>>>.......<<<<<<<........>>>>>>>.....
<<<<<<.>>>>>>..>>>................................
..<<....>>...............<<<<....>>>>.............
...................<<<<<<<<..<<<......<<<<<<<<.<<<
.....<<<<<<<.>>>>>>>......>>>.>>>.>>>>>..<<<<<<<<.
<<<<<<<<<<<<<<<...<<..<<<.<<.<<<<......<<<<<<..>>>
>>>.......>>>>>>.>>>........>>>>>>..>>>>>>>>>>>...
..>>.>>>>>>....>>>...>>>>>>>>..<<<<.<....>.>>>><<<
<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>.....>
>>....<<<<<...<<<..<<.........>>..>>>....>>>>>.<<.
>>....>>>>>>>>>>>>>..<<.<...<<<<<<...>>>>>>......>
.>>..<<<<<<....<.<<<.>>>.><<.<<<<<<<...<<<.<<<<<<<
<...>>>>>>...<<..>>....>>>>>>>>>>>>..>>..<<<<.<<<<
<.....>>>>>.>>>>....<<<......>>>...<<<<<<<...<<<((
(.......<<<<<.....<<..........<<<.<<<<<<<<<<.....<
.<<<<<.<<....<<.........>>....>>.........<<......>
>...>>>>>.>.>>>>>>>>>>>>>..........))).>>...>>>>>.
.>>>...>>>>>>><<<<<<.<<<.............<<..>>.......
...>>>>>>>>>....>>>>>>...<<<<<<<<.<<......<<<<.<<<
<....>>>>>>>>...>>>>>>>>>>........>>>>>><<.....<..
.<<<<...........>>>>..<<<<<...<<<<<<<....>>>>>>>..
....>>.>>>..<<<<<<.<<<<<<<..>>.>>>>>>>>>>>.......>
....>>...<<<<<<........>>>>>>....<<<<.<<<<......<<
<<<<.<<<<<<.<<<<<.<<<<<<<<<...<<<<<<<<<<.<<<<.....
.....<<<<<..<<<.>>>............>>>>>.>>>>.>>.>>>>>
>>>..>>>>>>>>>..>>>>>...<<<<<<<..<<<.........>>>>>
>>>>>........>>>>>.>.>>>>>>.............<<<<<<...<
..>...>>>>>>.......((...<<<<<<<<<<<..<.<.<<<<<....
..>>>>>...>.>.<<<.....))..>>>.....>>>>>....>>>>>>.
.<<<....>>>>>>>.>>>>..<<<<<<.<..<<<..<.<<<<<......
...........>>>>>.><<<<....<<<<.<<.<<<<<<<....>>>>>
..>>.>>...>>>>...>>>><<<<<...<<<<<...........>>>>>
...>>>>>.>>>...>.>>>>>>.<<<<<<<.<<..<<<<....<<<...
.....<<<<<.<<<<>>>>.>>>>>.......>>>...<<<..<<<<...
.<<<<.<<<<<<<......>>>>>>>>>>>...>>>>..>>>........
......>>>>..>>>>>>>>><<<<<.<.............>....<<.<
<<<<<<.<<.....<<<<....<<<<<......>>>>>...>>>>....>
>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fkt Principles of human pre-60 S biogenesis.
• Resolution: 2.81 Å
• Binding residue (original residue number in PDB): K2 A3 S4 G5 Q66 F68 E69 K70 P72 L73 R87 H91 M93 Y94 R95 R98 R111 G114 A115 R116 H117 R118 R120 R139 H146 D147 S148 H156 R157 R160 R161 H163 P165 R166 T169 K170 P172 N173 T174 F175 F176
• Binding residue (residue number reindexed from 1): K2 A3 S4 G5 Q66 F68 E69 K70 P72 L73 R87 H91 M93 Y94 R95 R98 R111 G114 A115 R116 H117 R118 R120 R139 H146 D147 S148 H156 R157 R160 R161 H163 P165 R166 T169 K170 P172 N173 T174 F175 F176

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0016020 membrane
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fkt, PDBe:8fkt, PDBj:8fkt
• PDBsum: 8fkt
• PubMed: 37410842
• UniProt: Q02543|RL18A_HUMAN Large ribosomal subunit protein eL20 (Gene Name=RPL18A)