Structure of PDB 5wlc Chain L9 Binding Site BS01

Receptor Information

>5wlc Chain L9 (length=175) Species: 1247190 (Saccharomyces cerevisiae BY4741)

KTYSTPKRPYESSRLDAELKLAGEFGLKNKKEIYRISFQLSKIRRAARDL
LTRDEKDPKRLFEGNALIRRLVRVGVLSEDKKKLDYVLALKVEDFLERRL
QTQVYKLGLAKSVHHARVLITQRHIAVGKQIVNIPSFMVRLDSEKHIDFA
PTSPFGGARPGRVARRNAARKAEAS

Ligand information

>5wlc Chain L1 (length=1025)

agauaguuaucugguugauccugccaguagucauaugcuugucucagcca
ugcagcucauuaaaucaguuaucguuuauuugauagucaugguauaacug
ugguaauucuagagcuaaaaucucgacccuuuggaagagauguauuuauu
agaucaaugucuucggacucuuugaugaauaacuuuucgaaucgcauggc
cuugugcuggcgaugguucauucaaauuucugcccuaucaacuuucgaug
guaggauaguggccuaccaugguuucaacggguaacggggaauaaggguu
cgauuccggagagggagccugagaaacggcuaccacauccaaggaagggc
aggcuuacccaauccuaauucagggagguagugacaauaaauaacgauac
agggcccauucgggucuuguaauuggaaugaguacaauguaaauaccuua
acgaggaacaauuggagggcaagucuggugccagcagccgcgguaauucc
agcuccaauagcguauauuagcucguaguugaacuauuaauagggacggu
cgggggcaucaguauucaauugucagaggugaaauucuuggauuuauuga
agacuaacuacugcgaaagcauuugccaaggacguuuucauuaaucaaga
acgaaaguuaggggaucgaagaugaucagauaccgucguagucuuaagga
guauggucgcaaggcugaaacuuaaaggaauugacggaagggcaccacca
ggaguggagccugcggcucaacacggggaaacucaccagguccagacaca
auauuugugggugguggugcaugugaugcccuuguucuggcgcgcgcuac
acugacggagccagcgagucuaaccuuggccgagaggucuugguaaaaac
uccgucggggaacgaggaauuccuaguaagcgcaagucaucagcuugcgu
ugauuacgucccugcccuuuguacacaccgcccgucgcuaguaccggaac
uaauuccguaggugaaccugcggaa

.............................<<.<<<<<<........<<<.
<<<..<<<.......<<<..<<..<<....<<<..............<<.
<<<.....>>>.>>.....<<<<<..<<....>>..>>>>>....<<<<.
...<<<<.<<<....>>>...>>>>...>>>>.........<<<<..<<<
.....>>>.>>>>....>>>...>>>>..>>>.<<<....<<.....<<<
<<<<<.......>>>>>>>>.>>......>>>....<<<...<.......
.>..>>>.>>>.<<.<<<..........>>>.>>.<.<<....>>.>.>>
>>>>..<<<....<<<.....>>>..>>>...............<<<<<<
<.<<<<<....>>>>>.>>>.>>>>......<<..<..<....>...>..
>>.........<<<<<<.......<<<....>>>................
..>>>>>>..>>>>>>>>..<.<<...<<<.<<...>>....<<<<<<.<
<...<<<<..<<<.<<<<<<.<...<<<......>>>......>.>>>>>
>.>>>......<<<....>>>..>>>>..>>.>>>.>>>...>>>...>>
.>.....<<<<<<...<...<<<<.........>>>>...>>>>>>>...
..............<<.........>>.....<<<<<<<<<<<<..<<.<
<<<<<..<<<.<<<<....................<<<<<......<<<<
<...>>>>>......<<<<<<..>>.>>>>.....>>>>>>>>>.>>>..
...<<<<<<<............<<<..<<<<....>>>>..>>>.....>
>>>>>>............>>>>>>....<<<<<<<.........>>>>>>
>......>>...>>>>>>>............>>>>>..<<<<.<<>>.>>
>>.<<<<<<<<<....>>>>>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5wlc The complete structure of the small-subunit processome.
• Resolution: 3.8 Å
• Binding residue (original residue number in PDB): K37 N38 K39 E41 R44 E72 H124 R126 V127 Q131 R132 H133 P163 P169 G170 R171 V172 A173 R175 N176
• Binding residue (residue number reindexed from 1): K28 N29 K30 E32 R35 E63 H115 R117 V118 Q122 R123 H124 P154 P160 G161 R162 V163 A164 R166 N167

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0042254 ribosome biogenesis
• GO:0042274 ribosomal small subunit biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:0030686 90S preribosome
• GO:0032040 small-subunit processome
• GO:0043226 organelle
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5wlc, PDBe:5wlc, PDBj:5wlc
• PDBsum: 5wlc
• PubMed: 28945246
• UniProt: O13516|RS9A_YEAST Small ribosomal subunit protein uS4A (Gene Name=RPS9A)