Structure of PDB 8hl5 Chain L30E Binding Site BS01

Receptor Information

>8hl5 Chain L30E (length=94) Species: 330779 (Sulfolobus acidocaldarius DSM 639)

FEGELKTLLRSGKVILGTRKTLKLLKTGKVKGVVVSSTLRQDLKDDIMTF
SKFSDIPIYLYKGSGYELGTLCGKPFMVSVIGIVDEGESKILEF

Ligand information

>8hl5 Chain A23S (length=2991)

aagccucccgguggauggcucggcucgggcaccgaagaagggcgcggcaa
gcagcgaaaugcucgggugaggcgcaagcagccguugaccccgagguccc
cuaaugggauauccugccggguuccggcgcccgguuuauaacuggguggg
aaccccccgaacggaaacaucuuaguagggggaggaaaagaaaucaauug
agauccccugaguaggggcgaccgaaagggggacagcccaaacuaaaccu
gccgaugauaagucgguggggaugugguguuacgaccucuagccugaggu
ugaccucggcuuuccuaaccuaucuagccgaacuccccuggaacgggggg
ccauagagggugaaagccccguaggcuaaagauagguggaaaguggcuag
agguagaguaccauccccugguuugggggugggaaguuaggggacacgug
ccucuaaggcuaaauaugucccgagaccgauagcaaacuaaguaccguga
gggaaagcugaaaagaaccccggaagggggagugcaaagagccugaaacc
gggugguuauacaggguguggcucgaaagaagugaacccuuccgaaggaa
aggggcgcaagcccuuaguacgaggaagggcgaucggggucacgccuuuc
gucuugaaacacgggccggggaguucacaucaguggcgagcuuaaggaga
uuaucuccgaaggcauagggaaaccaagugcccgcagccuaguuucuagg
cgaggggcagggucugucagggccugaagccacugaugugaggcuagaaa
ccgggcgaucuaguccggggcaggcugaaggugggggaaaccccacugga
gggccgaauagggguucugacgugcaauucguucccuugaccucggacua
ggggcaaaagaccaaucuagcccggugauagcuaguuccccccgaaaugc
guccuagcgcagccucccuaaaggcagcucgcgggguagagugacagauc
gggggcuccaggcgccuggggcuuccggucuaacuccgaacccacgagcg
ccgaagaaggggggagugggucacucggcguaagguuggguggcaaaagg
ggaacaacccagaccuggguuaaggccccaaagucccggcuaagugccaa
cgaaaaggcgucuccagccuuagacagcgggaaggugggcccagcagcag
ccauccucuaaggagugcguaacagcucacccgccgaggcuggaggcccu
aaagauuggucggggcucaagccgggcgccgagacccaggagggggucuc
uacugagauccucugguaggggggcgcugugaugggguagaagguggguc
gugagauccacuggacccgucacaggugcagaucccggcgguaguaacag
cgaaggggggugagaaucccccucgccggaagggcaaggguuucccggca
acguucgucagccgggaguuagcguccuaagguagggccuaauagguacc
uaccgaaagggaaagggguuaacauuccccugccucccggguaggugcgg
uaacgcaagccagacuccugacggauugggguagggagaguaggaccacc
guccuacccaagcacucaagcccuuggagagccguaacggugagaagagg
gcgaaggugugaugggccuuccguuaggaggguucuccugaucccuaguc
cccaugaaaagggagucuggaacgaucccgggagaccguaccuagaaccg
acacuggugcccgggugagaagcccaaggcgucugagggguaacccaggc
uagggaacucggcaaauuagccccguaacuucgggagaaggggugccuau
cgugguuuaacaaagccacgauaggucgcagugaccagagggaccugacu
guuuaauaaaaacauagguccccgcuagcccgaaaggguguguacggggg
cuaaauccuggccacuggugguugguuaaauccggguucaaccgggcgaa
gccccaccgaaggccggggguacccucuuaagguagccaaaugccuugcc
ggguaaguuccggcgcgcaugaauggaucaacgcggucccuacuguccca
gccuggggccucgugaacgcccugagccggugcacaguccggcaucuccc
uacaccgagagaagaccccguggagcuucaccgcagccuggcguuguccc
ucgggcguuuaugcguagaguaggugggaggggucgaaccuguccuuucg
ggggcaggggacccgaaagugaaacaccacccauggacgcucgagggacu
aaccucucgaaagagaggaacaacgucaggugggcgguucggcuggggcg
gcacucccgcgaaaagauaacacgggagcccaaaggucggcucaggcggu
acagaacgccgccguagagcgcaagggcaaaagccggccugacgugaccc
uuccaaguacggggucacgacgcgaaagcggggccuagcgaacgcucgug
cccccacacgugggggccgggcaugacagaaaaguuaccccggggauaac
agggucgucgcgggcgagagcucacaucgaccccgcgguuugcuacaucg
augucggcucuucccacccuggaggugcagcugccuccaaggguagggcu
gcccgcccguuaaaggggagcgugagcuggguuuagaccgucgcgagaca
ggucggacucuaaggguagggagugcggaccgcuugaggggaaggaaccc
cuaguacgagaggaacaggguuccggggccuccaguuuaccgguuguccg
guagggcaaugccgggcagccgcgcccugagggguaaccgcugaaagcau
cuaagcgggaaccccuccccuaaaagaggcggucaggcgccggggccuuc
cccuagaagaggggguugaugggguggggauguaagcuccaagguuguaa
gaccgaggaguuuaguccgccacucccaaucaggcuccccc

<.<<<.<<<<<<.....<<..<<<<<<<<<<<<.....<<<<<<<.((..
((.>>>.....<<<<<<...<<<))..)).>>>......>>>>>>...>>
>>...<<<....>>><<<<<<.>>>>>><<<<<<<<...>>>>>>>>>>.
..<<<<<<....<<<....>>>.....>>>>>.>.........<<....>
>..<<<<<<.....<<.....>>....>>>>>>..<<<...<<<...<<<
<<<<<<.....>>>>>>>>>.....>>>......<<<<<<<<<<<<<<<<
..>>>>>><<<<<<<...<<<<<<<.<<<.....<<<<......>>>>.(
(......<<<.....))>>>...>>>...>>>>>>>.>>>>>>>>>>>>>
>>>>......<<<<<<<<......>>>>>>>>.......<<<<.((((((
>>>>...>>>......>>.>>>>>>.>>................<<....
>>....>>.........<<<<.....>>>>.................>>>
>>>.>>>>......<<<<<<<<<<....<<......<<<<<<<...<...
.<<<<<....>>>>>.....>..>>>>>>>...>>.>>>>>>>>>>..<<
<<........>>>><<<<<<...<<<<<<<<<<<<<<..<<<...<<<<<
...>>>>>...>>>...<<....>>...<<<<<...<<<<.......>>>
>...>>>>><<<<<<....>>>>>>...>>>>>>>>>>>>>>.<<<<..<
<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>...
..>>>....<<<<<....<<........>>..>>>>>...>>>>>>>>>.
..<<.......>>......>>>>>>...>..>>>>..<<<<<<....<<<
<<....>>>>><<<<<<<<...<<<.<<<<<.<<<...<<<....<<<<<
<<<<<<<<<<<<<>>>>>>>>>>>>>>>>>>..>>.>...>>>.>>>>>>
>>......>>>>>>.>>..<<<<<<.<<<.....>>>.>>>>>>....<<
<......>>>...<<<<<<<...<<<(((.....<<<<<<.....<<...
.....<<<.<<<<<<<<<<.......<<<<..<<....<<.........>
>....>>.........<<......>>....>>>>...>>>>>>>>>>>>>
...........)))>>....>>>>>>.>>>...>>>>>>><<<<<<<<<<
....>>>>>>>>>>....>>>>>><<<<<<<<<.<<<.....<<<<<<<<
....>>>>>>>>...>>>>>>>>>>.>>.....>>>>>><......<<..
....<<<<<<.......>>>>>><<<<....<<<<..<<...<<<<<<..
..<.....>..>>>>>>....<.{<<<...<<<<<<.<<.....>>..>>
>>>>....>>>..<<<<<........>>>>>.><<<<<<<<.....<<<.
...>>>...<<<<<<<<<<...<<<<<<<<<..<<<<<<<<<<<<<.[[[
>>>>>>>....<<<<.<..<<<<<<.....<<<<...>>>>.....>>>>
>>...>>>>>.....<<<<<<<....>>>>>>>>>>>>>...>>>>>>>>
>.........>>>>>>>>>>......>>>>>>>>.}....>>.....<<.
.....>>.>>>><<<<.....>>>>..>>>>...<<<<.....<<<<<<<
<.<<...............<<<<<.....<..>......>>>>><<<<<<
<<<<<<<......>>>>>>>>>>>>>............<<<<<<<<<..<
<<.........>>>..<<<<<<<....<<<<....>>>>......>>>>>
>>..<<<<.<<<..<<<<<<<..<<<<...<<<<<......>>>>>...>
>>>>>>>>..>>>>><<<<<..>>>>>....................<<<
<<<.....>>>>>>..........>>>>...>>.>>>>>>>.....>>.>
>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<
<<<.<<..<<......<<<<.<<<<<<..<<<<<.<<<<<<<<<.<<<<<
<<<<<<<<<.<<<.........<<<<<..<<<...........<.<....
.>.>.......>>>............>>>>>>>>.>>>>>>>>>>>>>>.
..<<<<<<....>>>>>>....>>>>>>>>>..>>>>>...<<<.....>
>>..<<<<<.............>>>>>.(<<...<<<<<<<...<<<<<<
.......>>>>>>.....<<)..(((>>....>>>>>>>...<<<<<<<<
<.........>>>>>>>>>.<<<....>>>.>>))).......<<<<<.<
<<<<..))))))>>>>>>>>>>..........>>>>..>>>>>>......
.<<<<...<<<<<<...<<.......>>...>>>>>>....>>>.>....
.....<<<.<.<<<<..<<<<<<<<<......>>>>>>>..>>..<<<<.
....>>>>......>>>>...>...>>>.......<<<<<<<....>>>.
>>>>....>>...>>>>>>>>>>....<<<<<<<..<<<<<..<<<<<<<
.................>>>>>>><<<<<.....<<...<<<<<<<<<<]
]].>>>>>..>>>>>...>>...>>>>>.<<<<<...<<<<<........
...>>>>>...>>>>>>>>>>......>>>>>>>.<<..>><<<<<<<<<
<<<.......>>>>>....<<<<<<<<<<<<....<<<<<..<<<<....
>>>>..>>>>>...>>>>.>>>>>.>>>...>>>>>>>...

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8hl5 Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome
• Resolution: 5.72 Å
• Binding residue (original residue number in PDB): L20 G21 T22 R23 K24 R44 K78 P79 F80 S83
• Binding residue (residue number reindexed from 1): L16 G17 T18 R19 K20 R40 K74 P75 F76 S79

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8hl5, PDBe:8hl5, PDBj:8hl5
• PDBsum: 8hl5
• PubMed: 37604686
• UniProt: P11522|RL30E_SULAC Large ribosomal subunit protein eL30 (Gene Name=rpl30e)