Structure of PDB 8hkv Chain L23P Binding Site BS01

Receptor Information

>8hkv Chain L23P (length=81) Species: 330779 (Sulfolobus acidocaldarius DSM 639)

IIKEFLATEKAVKLIESQNTLTIIVNRNTTKKDIKNEVEKLFSVKVEKIN
TLITPKGEKKAYVKLKQEYKASDVAHKLGML

Ligand information

>8hkv Chain A23S (length=2989)

aagccucccgguggauggcucggcucgggcaccgaagaagggcgcggcaa
gcagcgaaaugcucgggugaggcgcaagcagccguugaccccgagguccc
cuaaugggauauccugccggguuuccggcucccgguuuauaacugggugg
gaaccccccgaacggaaacaucuuaguagggggaggaaaagaaaucaauu
gagauccccugaguaggggcgaccgaaagggggacagcccaaacuaaacc
ugccgaugauaagucgguggggaugugguguuacgaccucuagccugagg
uugaccucggcuuuccuaaccuaucuagccgaacuccccuggaacggggg
gccauagagggugaaagccccguaggcuaaagauagguggaaaguggcua
gagguagaguaccauccccugguuugggggugggaaguuaggggacacgu
gccucuaaggcuaaauaugucccgagaccgauagcaaacuaaguaccgug
agggaaagcugaaaagaaccccggaagggggagugcaaagagccugaaac
cgggugguuauacaggguguggcucgaaagaagugaacccuuccgaagga
aaggggcgcaagcccuuaguacgaggaagggcgaucggggucacgccuuu
cgucuugaaacacgggccggggaguucacaucaguggcgagcuuaaggag
auuaucuccgaaggcauagggaaaccaagugcccgcagccuaguuucuag
gcgaggggcagggucugucagggccugaagccacugaugugaggcuagaa
accgggcgaucuaguccggggcaggcugaaggugggggaaaccccacugg
agggccgaauagggguucugacgugcaauucguucccuugaccucggacu
aggggcaaaagaccaaucuagcccggugauagcuaguuccccccgaaaug
cguccuagcgcagccucccuaaaggcagcucgcgggguagagugacagau
cgggggcuccaggcgccuggggcuuccggucuaacuccgaacccacgagc
gccgaagaaggggggagugggucacucggcguaagguuggguggcaaaag
gggaacaacccagaccuggguuaaggccccaaagucccggcuaagugcca
acgaaaaggcgucuccagccuuagacagcgggaaggugggcccagcagca
gccauccucuaaggagugcguaacagcucacccgccgaggcuggaggccc
uaaagauuggucggggcucaagccgggcgccgagacccaggagggggucu
acugagauccucugguaggggggcgcugugaugggguagaaggugggucg
ugagauccacuggacccgucacaggugcagaucccggcgguaguaacagc
gaaggggggugagaaucccccucgccggaagggcaaggguuucccggcaa
cguucgucagccgggaguuagcguccuaagguagggccuaauagguaccu
accgaaagggaaagggguuaacauuccccugccucccggguaggugcggu
aacgcaagccagacuccugacggauugggguagggagaguaggaccaccg
uccuacccaagcacucaagcccuuggagagccguaacggugagaagaggg
cgaaggugugaugggccuuccguuaggaggguucuccugaucccuagucc
ccaugaaaagggagucuggaacgaucccgggagaccguaccuagaaccga
cacuggugcccgggugagaagcccaaggcgucugagggguaacccaggcu
agggaacucggcaaauuagccccguaacuucgggagaaggggugccuauc
gugguuuaacaaagccacgauaggucgcagugaccagagggaccugacug
uuuaauaaaaacauagguccccgcuagcccgaaaggguguguacgggggc
uaaauccugccacuggugguugguuaaauccggguucaaccgggcgaagc
cccaccgaaggccggggguacccucuuaagguagccaaaugccuugccgg
guaaguuccggcgcgcaugaauggaucaacgcggucccuacugucccagc
cuggggccucgugaacgcccugagccggugcacaguccggcaucucccua
caccgagagaagaccccguggagcuucaccgcagccuggcguugucccuc
gggcguuuaugcguagaguaggugggaggggucgaaccuguccuuucggg
ggcaggggacccgaaagugaaacaccacccauggacgcucgagggacuaa
ccucucgaaagagaggaacaacgucaggugggcgguucggcuggggcggc
acucccgcgaaaagauaacacgggagcccaaaggucggcucaggcgguac
agaacgccgccguagagcgcaagggcaaaagccggccugacgugacccuu
ccaaguacggggucacgacgcgaaagcggggccuagcgaacgcucgugcc
cccacacgugggggccgggcaugacagaaaaguuaccccggggauaacag
ggucgucgcgggcgagagcucacaucgaccccgcgguuugcuacaucgau
gucggcucuucccacccuggaggugcagcugccuccaaggguagggcugc
ccgcccguuaaaggggagcgugagcuggguuuagaccgucgcgagacagg
ucggacucuaaggguagggagugcggaccgcuugaggggaaggaaccccu
aguacgagaggaacaggguuccggggccuccaguuuaccgguuguccggu
agggcaaugccgggcagccgcgcccugagggguaaccgcugaaagcaucu
aagcgggaaccccuccccuaaaagaggcggucaggcgccggggccuuccc
cuagaagaggggguugaugggguggggauguaagcuccaagguuguaaga
ccgaggaguuuaguccgccacucccaaucaggcuccccc

..<<<.<<<<<<....<<<..<<<<<<<<<<<<<....<<<<<<<.((..
((.>>>.....<<<<<<...<<<))..)).>>>......>>>>>>...>>
>>...<<<....>>><<<<<....>>>>>..<<<<<.....>>>>>.>>>
...<<<<<<....<<<....>>>.....>>>>>.>.........<<....
>>...<<<<<.....<<.....>>....>>>>>...<<<...<<<...<<
<<<<<<<.....>>>>>>>>>.....>>>......<<<<<<<<<<<<<<<
<..>>>>>><<<<<<<..<<<<<<<<.<<<.....<<<<......>>>>.
((......<<<.....))>>>...>>>...>>>>>>>>>>>>>>>>>>>>
>>>>>......<<<<<<<<......>>>>>>>>......<<<<<.(((((
(>>>>>..>>>......>>.>>>>>>.>>................<<...
.>>....>>.........<<<<.....>>>>............>....>>
>>>>.>>>.......<<<<<<<<<<....<<......<<<<<<<...<..
..<<<<<....>>>>>.....>..>>>>>>>...>>.>>>>>>>>>>..<
<<<........>>>><<<<<<....<<<<<<<<<<<<<..<<<...<<<<
<...>>>>>...>>>...<<....>>...<<<<<...<<<<.......>>
>>...>>>>><<<<<<....>>>>>>...>>>>>>>>>>>>>..<<<<..
.<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>..
...>>>....<<<<<....<<........>>..>>>>>...>>>>>>>>>
...<<.......>>......>>>>>>......>>>>..<<<<<<....<<
<<<....>>>>><<<<<<<<...<<<.<<<<<.<<<...<<<....<<<<
<<<<<<<<<<<<<<>>>>>>>>>>>>>>>>>>..>>.>...>>>.>>>>>
>>>......>>>>>>.>>..<<<<<<<<<<.....>>>>>>>>>>....<
<<......>>>...<<<<<<<...<<<(((.....<<<<<<.....<<..
......<<<.<<<<<<<<<<.......<<<<<.<<<...<<.........
>>...>>>.........<<......>>...>>>>>...>>>>>>>>>>>>
>...........)))>>....>>>>>>.>>>...>>>>>>><<<<<<<.<
...>.>.>>>>>>....>>>>>><<<<<<<<<.<<<.....<<<<<<<<.
...>>>>>>>>...>>>>>>>>>>.>>.....>>>>>><......<<...
...<<<<<<.......>>>>>><<<<....<<<<<<<<...<<<<<<...
.<.....>..>>>>>>....(.<<<<...<<<<<<<<<.....>>>.>>>
>>>....>>....<<<<........>>>>..)<<<<<<<<.....<<<..
..>>>...<<<<<<<<<<...<<<<<<<<<..<<<<<<<<<<<<..[[[.
>>>>>><...<<<<<...<<<<<<.....<<<<...>>>>.....>>>>>
>...>>>>>....><<<<<<<....>>>>>>>>>>>>>...>>>>>>>>>
.........>>>>>>>>>>......>>>>>>>>>>....>>>....<<..
....>>>>>>><<<<.....>>>>..>>>>...<<<<.....<<<<<<<<
.<<...............<<<<<....<<..>>.....>>>>><<<<<<<
<<<<<<......>>>>>>>>>>>>>............<<<<<<<<<..<<
<.........>>>..<<<<<<<....<<<<....>>>>......>>>>>>
>..<<<<.<<..<<<<<<<..<<<....<<<<<......>>>>>....>>
>>>>>>..>>>>.<<<<<..>>>>>....................<<<<<
<.....>>>>>>..........>>>>...>>.>>>>>>>.....>>.>>>
>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<
<.<<..<<......<<<<.<<<<<<..<<<<<.<<<<<<<<<.<<<<<<<
<<<<<<<<<<<.........<<<<<..<<<......<.<<.<<<.....>
>>>.>>...>>>............>>>>>>>>>>>>>>>>>>>>>>>...
<<<<<<....>>>>>>....>>>>>>>>>..>>>>>...<<<.....>>>
..<<<<<.............>>>>>..<<...<<<<<<<...<<<<<<..
.....>>>>>>.....<<...(((>>....>>>>>>>...<<<<<<<<<.
........>>>>>>>>>.<<<....>>>.>>))).......<<<<<.<<<
<<..))))))>>>>>>>>>>..........>>>>..>>>>>>.......<
<<<.<.<<<<<<...<<.......>>...>>>>>>...>>>>.>......
.<.<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>..<<<<...
..>>>>......>>>>>..>...>>>..>....<<<<<<<....>>>.>>
>>....>>...>>>>>>>>>>....<<<<<<<..<<<<<..<<<<<<<..
...............>>>>>>><<<<<.....<<...<<<<<<<<<<]]]
.>>>>>..>>>>>...>>...>>>>>.<<<<<...<<<<<..........
.>>>>>...>>>>>>>>>>......>>>>>>>.<<..>><<<<<<<.<<<
<.......>>>>.....<<<<<<<<<<<<....<<<<<..<<<<....>>
>>..>>>>>...>>>>.>>>>>.>>>...>>>>>>>...

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8hkv Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome
• Resolution: 4.94 Å
• Binding residue (original residue number in PDB): T9 E10 Q19 T30 K32 N51 T52 L53 I54 K60 K61 Y63 K65
• Binding residue (residue number reindexed from 1): T8 E9 Q18 T29 K31 N50 T51 L52 I53 K59 K60 Y62 K64

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8hkv, PDBe:8hkv, PDBj:8hkv
• PDBsum: 8hkv
• PubMed: 37604686
• UniProt: Q4JB42|RL23_SULAC Large ribosomal subunit protein uL23 (Gene Name=rpl23)