Structure of PDB 7azs Chain L23B Binding Site BS01

Receptor Information

>7azs Chain L23B (length=94) Species: 300852 (Thermus thermophilus HB8)

MKTAYDVILAPVLSEKAYAGFAEGKYTFWVHPKATKTEIKNAVETAFKVK
VVKVNTLHVRGKKKRLGRYLGKRPDRKKAIVQVAPGQKIEALEG

Ligand information

>7azs Chain 23SB (length=2875)

gucaagaugguaagggcccacgguggaugccucggcacccgagccgauga
aggacguggcuaccugcgauaagccagggggagccgguagcgggcgugga
ucccuggauguccgaaugggggaacccggccggcgggaacgccggucacc
gcgcuugcgcggggggaaccuggggaacugaaacaucucaguacccagag
gagaggaaagagaaaucgacucccugaguagcggcgagcgaaaggggacc
agccuaaaccguccggcuuguccgggcggggucguggggcccucggacac
cgaauccccagccuagccgaagcuguugggaagcagcgccagagagggug
aaagccccguaggcgaaagguggggggauaggugaggguacccgaguacc
ccgugguucguggagccaugggggaaucugggcggaccaccgccuaaggc
uaaguacuccgggugaccgauagcgcaccaguaccgugagggaaagguga
aaagaaccccgggaggggagugaaauagagccugaaaccgugggcuuaca
agcagucacggccccgcaagggguuguggcgugccuauugaagcaugagc
cggcgacucacggucgugggcgagcuuaagccguugaggcggaggcguag
ggaaaccgaguccgaacagggcgcaaacggcccgaaaaguccgcggccgu
ggacccgaaaccgggcgagcuagcccuggccagggugaagcuggggugag
acccaguggaggcccgaaccggugggggaugcaaaccccucggaugagcu
ggggcuaggagugaaaagcuaaccgagcccggagauagcugguucucccc
gaaaugacuuuagggucagccucaggcgcugacuggggccuguagagcac
ugauagggcuagggggcccaccagccuaccaaacccugucaaacuccgaa
gggucccagguggagccugggagugagggcgcgagcgauaacguccgcgu
ccgagcgcgggaacaaccgagaccgccagcuaaggcccccaagucugggc
uaagugguaaaggauguggcgccgcgaagacagccaggauguugaucauu
uaaagagugcucacuggucgaguggcgccgcgccgaaaaugaucggggcu
uaagcccagcgccgaagcugcgggucugggggaugaccccaggcgguagg
ggagcguucccgaugccgaugaaggccgacccgcgagggcggcuggaggu
aagggaagugcgaaugccggcaugaguaacgauaaagagggugagaaucc
cucucgccguaagcccaaggguuccuacgcaauggucgucagcguagggu
uaggcgggaccuaaggugaagccgaaaggcguagccgaagggcagccggu
uaauauuccggcccuucccgcaggugcgauggggggacgcucuaggcuag
ggggaccggagccauggacgagcccggccagaagcgcagggugggaggua
ggcaaauccgccucccaacaagcucugcgugguggggaagcccguacggg
ugacaaccccccgaagccagggagccaagaaaagccucuaagcacaaccu
gcgggaacccguaccgcaaaccgacacaggugggcgggugcaagagcacu
caggcgcgcgggagaacccucgccaaggaacucugcaaguuggccccgua
acuucgggagaaggggugcucccuggggugaugagccccggggagccgca
gugaacaggcucuggcgacuguuuaccaaaaacacagcucucugcgaacu
cguaagaggagguauagggagcgacgcuugcccggugccggaaggucaag
gggaggggugcaagccccgaaccgaagccccggugaacggcggccguaac
uauaacgguccuaagguagcgaaauuccuugucggguaaguuccgaccug
cacgaaaagcguaacgaccggagcgcugucucggcgagggacccggugaa
auugaacuggccgugaagaugcggccuacccguggcaggacgaaaagacc
ccguggagcuuuacugcagccugguguuggcucuuggucgcgccugcgua
ggauaggugggagccugugaacccccgccuccgggugggggggaggcgcc
ggugaaauaccacccuggcgcggcugggggccuaacccucggaugggggg
acagcgcuuggcgggcaguuugacuggggcggucgccuccuaaaagguaa
cggaggcgcccaaagguccccucaggcgggacggaaauccgccggagagc
gcaaggguagaagggggccugacugcgaggccugcaagccgagcaggggc
gaaagccgggccuagugaaccgguggucccguguggaagggccaucgauc
aacggauaaaaguuaccccggggauaacaggcugaucucccccgagcguc
cacagcggcggggagguuuggcaccucgaugucggcucgucgcauccugg
ggcugaagaaggucccaaggguugggcuguucgcccauuaaagcggcacg
cgagcuggguucagaacgucgugagacaguucggucucuauccgccacgg
gcgcaggaggcuugaggggggcucuuccuaguacgagaggaccggaaggg
acgcaccucugguuucccagcugucccuccaggggcauaagcuggguagc
caugugcggaagggauaaccgcugaaagcaucuaagcgggaagcccgccc
caagaugaggccucccacggcgucaagccgguaaggacccgggaagacca
cccgguggaugggccggggguguaagcgccgcgaggcguugagccgaccg
gucccaaucguccgaggucuugacc

<<<<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<<....>>>>>>>>.<<
<<<<..>>>>>>.>>....<<<<<.....<<....>>......>>>>>..
.........<<....>>...<<<<<.....<<.....>>....>>>>>..
.<<<....<<<<<<<<.....>>>>>>>>......<<<<<<<<<....<<
<...<<<<<.<<<<.<<<...<<<<<......>>>>>((......<<<..
...))>>>...>>>...>>>>..>>>>>..>>>>>>>>>.>>>.....<<
<<<<<<<.......>>>>>>>>>......<<<<<..((((>>>>>..>>>
......>>.>>>>>>.>>...............<<....>>....>>...
......<<<<....>>>>..................>>>>>>>>>>>...
....<<<<<<<<<<<....>>>>>>>>>>>...<............>.<<
<<<<....<<<<<<<<<<<<<..<<<...<<<.....>>>...>>>...<
<....>>..<<<<.....>>>>.....<<...>>....>>>>>>>>>>>>
>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<.....
.>>>>>>.....>>>...<<<..<<<<........>>>>.>>>....>>>
>>>>>>...<<<.....>>>......>>>>>>......>.>>..<<<<<<
....<<<<<....>>>>><<<<<<<<<.....<<<<<<<<<...<<<...
<<<<<<<<...<<<.<........>.>.>>...>>>>>>>>..>>.>...
>>>>>>>>>.....>>>>>>>.>>..<<<<<<<.<<......>>.>>>>>
>>.....<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<
...........<<..<<<<<<<<<......<.<<<<<.<<.......>>.
.............>>>>>.>..>>>>>>>>>.>>..........)))...
...>>>>>>.>>>...>>>>>>><<<<<<<<.....>>>>>>>>....>>
>>>>..<<<<<...<<<......<<<<<.<<<....>>>>>>>>...>>>
..>>>>>........>>>>>><......<<.....<<<<<<.......>>
>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>..
..((<<<<.<<...<<<...<<<....>>>...>>>...>>...<<<<<.
.......>>>>>))<<<<<<<<<<<<<...<<<<....<<<<<.<<<..<
<<<<.<<<<.<<..[[[[...>>>>>><<<...<<<<<<<<<<<<<<<<.
<<.....>>>>>>>>>.....>>>>>>>>>..>>><...<<<<....>>>
>.....>>>>>>...>>>..>>>>>.........>>>>...>>...>>>>
>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<<<....>>>>>
..>>>>..<<<<....<<<<<<<<.<<.........<.....<<<<<...
............>>>>><<<<<<<<<<<<.....>>>>>>>>>>>>....
........<<<<<<<<<..<<<.........>>>..<<<<<<<.....<<
<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<<<...<
<...<<<<<....>>>>>...>>...>>>>>>>>..>>>>><<<<<<...
....>>>>>>........<...........<<<<<<.....>>>>>>...
..>....>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>...
..>.....<<<<<........>>>>>..<<<<<<<<.<<<.........<
<<<.<<<<<<..<<<<<.<<<.<<<<<.<<<<<<<<<<<<<<<<......
.....<<.....<<.......<<<<.<<<....>>>.>>>>....>>...
............>>..>>>>>>>>>>>>>>>>...<<<<<....>>>>>.
...>>>>>.>>>..>>>>>..<<<<.....>>>><<<<<<..........
.>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<
...(((>>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<<
....>>>.>>))).......<<<<<<<.<<..))))..>>>>>>>>>...
..........>>>>..>>>>>>.......<<<<.<.<<<<<<...<<...
....>>...>>>>>>...>>>>.>.......<<<<<.<<<<<<..<<<<<
<<<<......>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..>
...>>>.>>....<<<<<<<....>>>.>>>>........>>>>>>>>>>
>....<<<<<<<...<<<<..<<<<<<<...............>>>>>>>
.<<<<<...<<<<...<<<<<<<<<<<]]]]>>>>>>...>>>>>...>>
>>.>>>>>...<<<....<<<<.............>>>>....>>>.>>>
>.......>>>>>>>..<<<<.....>>>>....<<<<<<<<<.......
>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>...>>>..>>>
>>.>>>...>>>>....>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7azs Discovery of natural-product-derived sequanamycins as potent oral anti-tuberculosis agents.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): K2 S14 K16 K25 H31 T35 K36 T37 E38 K40 N41 E44 K50 K53 N55 T56 L57 H58 R60 K62 K63 R65 L66 Y69 L70 G71 K72 R73 P74 D75 K78
• Binding residue (residue number reindexed from 1): K2 S14 K16 K25 H31 T35 K36 T37 E38 K40 N41 E44 K50 K53 N55 T56 L57 H58 R60 K62 K63 R65 L66 Y69 L70 G71 K72 R73 P74 D75 K78

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7azs, PDBe:7azs, PDBj:7azs
• PDBsum: 7azs
• PubMed: 36827973
• UniProt: Q5SHP0|RL23_THET8 Large ribosomal subunit protein uL23 (Gene Name=rplW)